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The map label for this gene is tag [H]

Identifier: 218709966

GI number: 218709966

Start: 2131404

End: 2131970

Strand: Reverse

Name: tag [H]

Synonym: VS_1985

Alternate gene names: 218709966

Gene position: 2131970-2131404 (Counterclockwise)

Preceding gene: 218709967

Following gene: 218709965

Centisome position: 64.62

GC content: 43.92

Gene sequence:

>567_bases
ATGGAAACAAACACTGAAGGTAGAACGTGTGCATGGGCACTGAAGCACGAACTGGAAAGGGAGTACCACGACGCAGAGTG
GGGCGTACCGGTTTATGATGACCAAGTTTTGTTTGAGTTCATTACCTTAGAAGGGGCACAAGCAGGCCTTAGCTGGATTA
CGATCCTTAAAAAGCGCGAAGGCTATCGTGCTGCCTTCGATAATTACGATCTAAACAAGTTAGCCGAATTGAATGAAGAC
AATGTGCCACACGTCATAGAGAACTTTGATGTGGTTAAGCATAAAGGCAAGATTGCCTCTGTGTATAACAACGCTCGAGC
AACGCTTGAACTGCAGAAAGAGTTTGGTTCTCTTTCAAACGCCTTATGGCAATTTGTCGATAACAAGGTGATCGAAAACC
ACTGGATTGAGATGACTCAAGTTCCCGCTTCTACCGAGCAATCCAAAGCGATGAGTAAGTTTTTGAAGAAGAGAGGCTTT
AAGTTTGTTGGGGAAACAATCTGTTACGCGTTAATGCAAGCGACAGGTATGGTTAATGACCATTTAGAAGGTTGCCCACA
TAAATAA

Upstream 100 bases:

>100_bases
CCATAAAATTGAAGTCTCGTAAGACGGGTGAGTCTTTGAATTAATGCCCCTGTTTAGGGGCATTATCATTTCTGAGTGAT
TTAAAATAGGTAGCAATAAC

Downstream 100 bases:

>100_bases
AGGTGTTATTTCTCGACGCGAAATAATTGTGTGTAAATTGAAAAATTCACTGGTCATTTATAAACTTTTCAATATTATAT
GCGACCAAATTCAAATGTAG

Product: DNA-3-methyladenine glycosylase

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase I, constitutive; TAG I; DNA-3-methyladenine glycosidase I; DNA-3-methyladenine glycosylase I [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
METNTEGRTCAWALKHELEREYHDAEWGVPVYDDQVLFEFITLEGAQAGLSWITILKKREGYRAAFDNYDLNKLAELNED
NVPHVIENFDVVKHKGKIASVYNNARATLELQKEFGSLSNALWQFVDNKVIENHWIEMTQVPASTEQSKAMSKFLKKRGF
KFVGETICYALMQATGMVNDHLEGCPHK

Sequences:

>Translated_188_residues
METNTEGRTCAWALKHELEREYHDAEWGVPVYDDQVLFEFITLEGAQAGLSWITILKKREGYRAAFDNYDLNKLAELNED
NVPHVIENFDVVKHKGKIASVYNNARATLELQKEFGSLSNALWQFVDNKVIENHWIEMTQVPASTEQSKAMSKFLKKRGF
KFVGETICYALMQATGMVNDHLEGCPHK
>Mature_188_residues
METNTEGRTCAWALKHELEREYHDAEWGVPVYDDQVLFEFITLEGAQAGLSWITILKKREGYRAAFDNYDLNKLAELNED
NVPHVIENFDVVKHKGKIASVYNNARATLELQKEFGSLSNALWQFVDNKVIENHWIEMTQVPASTEQSKAMSKFLKKRGF
KFVGETICYALMQATGMVNDHLEGCPHK

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=176, Percent_Identity=47.7272727272727, Blast_Score=187, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 21572; Mature: 21572

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METNTEGRTCAWALKHELEREYHDAEWGVPVYDDQVLFEFITLEGAQAGLSWITILKKRE
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCC
GYRAAFDNYDLNKLAELNEDNVPHVIENFDVVKHKGKIASVYNNARATLELQKEFGSLSN
CCHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHH
ALWQFVDNKVIENHWIEMTQVPASTEQSKAMSKFLKKRGFKFVGETICYALMQATGMVND
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHH
HLEGCPHK
HCCCCCCC
>Mature Secondary Structure
METNTEGRTCAWALKHELEREYHDAEWGVPVYDDQVLFEFITLEGAQAGLSWITILKKRE
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCC
GYRAAFDNYDLNKLAELNEDNVPHVIENFDVVKHKGKIASVYNNARATLELQKEFGSLSN
CCHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHH
ALWQFVDNKVIENHWIEMTQVPASTEQSKAMSKFLKKRGFKFVGETICYALMQATGMVND
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHH
HLEGCPHK
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536912; 3520491; 8041620; 9278503 [H]