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The map label for this gene is nrdB [H]

Identifier: 218709927

GI number: 218709927

Start: 2085365

End: 2086498

Strand: Direct

Name: nrdB [H]

Synonym: VS_1940

Alternate gene names: 218709927

Gene position: 2085365-2086498 (Clockwise)

Preceding gene: 218709926

Following gene: 218709928

Centisome position: 63.21

GC content: 43.74

Gene sequence:

>1134_bases
ATGGCTTACAGTACTTTTTCTCAACAAAAAAATGACCAACTAAAAGAACCAATGTTCTTGGGTCAGTCGGTAAACGTTGC
ACGTTACGACCAACAAAAATTTGAAATCTTCGAAAAGCTTATCGAGAAGCAGCTTTCTTTCTTCTGGCGCCCAGAAGAGG
TCGACGTGTCTAGTGACCGTATCGACTACAACAAGCTTCCTGAGCATGAAAAGCACATCTTCATCTCTAACTTGAAGTAC
CAAACGCTTCTAGATTCTATCCAAGGCCGCAGCCCTAACGTTGCCCTACTTCCATTGGTATCACTACCAGAAGTTGAAAC
TTGGATTGAGACATGGTCATTCTCTGAAACGATTCACTCTCGTTCTTACACGCACATCATCCGTAACATCGTAAATGATC
CAGGCGTAGTATTCGACGACATCGTTGAAAACGAAGAGATCCTGAAGCGTGCTAAAGACATCGCATTTTACTACGACGAT
TTGATCAAGCTGACGGCTGACTACCACCGCTACGGTGAAGGCAACCACAACATCAACGGCGAAGACGTTAAAATTTCACT
TCACGACCTGAAGAAGAAGCTTTACGTATGTCTAATGTCTGTAAACGCACTGGAAGCGATTCGTTTCTACGTGAGTTTTG
CATGTTCATTCGCATTCGCTGAACGTGAGCTAATGGAAGGTAACGCGAAGATCATCAAGCTAATCGCTCGTGATGAAGCA
CTTCACCTTACTGGTACACAGCACATGATCAACTTGCTGCGTAATGGCCAAGATGACTTCGAATTCATGCAGATCGCTGA
AGAGTGTAAGCAAGAATGTTTCGACCTATTCAAAGAAGCAGCAGAGCAAGAGAAAGAGTGGGCAGAATACCTATTCAAAG
ATGGCTCTATGATTGGTCTAAACAAAGACATTCTTTGCCAATACGTTGAGTACATTACTAACATCCGTATGCAGGCTGTT
GGTCTAGGTACCGCTTACCCAGAAGCAACGTCGAACCCAATTCCATGGATCAACGCTTGGTTGTCTTCAGATAACGTTCA
AGTTGCTCCACAAGAAGCTGAAATCAGTTCTTACCTAGTTGGCCAAATCGACAACGAAGTAAAAGCTGACGACTTTGAAG
GGTTTGAGCTGTAA

Upstream 100 bases:

>100_bases
GCAATAAACATTAAGACCCCAGAGAAGAGTTATCTTCTCTGGGTTTAAAGCATTAACAGGGTCCGTTTTATTGCGGGTCT
TTAAAGGATTTGAGGCATTT

Downstream 100 bases:

>100_bases
TGCCAACAATCAAAATCAACAAGCTGACTTCTATTGAATCTAACCCTTCAAATACTCTATTGGAAACAATGGAACAAGCA
GGGTTAGAGCCAGAATACAA

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Protein B2; Protein R2; Ribonucleotide reductase 1 [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MAYSTFSQQKNDQLKEPMFLGQSVNVARYDQQKFEIFEKLIEKQLSFFWRPEEVDVSSDRIDYNKLPEHEKHIFISNLKY
QTLLDSIQGRSPNVALLPLVSLPEVETWIETWSFSETIHSRSYTHIIRNIVNDPGVVFDDIVENEEILKRAKDIAFYYDD
LIKLTADYHRYGEGNHNINGEDVKISLHDLKKKLYVCLMSVNALEAIRFYVSFACSFAFAERELMEGNAKIIKLIARDEA
LHLTGTQHMINLLRNGQDDFEFMQIAEECKQECFDLFKEAAEQEKEWAEYLFKDGSMIGLNKDILCQYVEYITNIRMQAV
GLGTAYPEATSNPIPWINAWLSSDNVQVAPQEAEISSYLVGQIDNEVKADDFEGFEL

Sequences:

>Translated_377_residues
MAYSTFSQQKNDQLKEPMFLGQSVNVARYDQQKFEIFEKLIEKQLSFFWRPEEVDVSSDRIDYNKLPEHEKHIFISNLKY
QTLLDSIQGRSPNVALLPLVSLPEVETWIETWSFSETIHSRSYTHIIRNIVNDPGVVFDDIVENEEILKRAKDIAFYYDD
LIKLTADYHRYGEGNHNINGEDVKISLHDLKKKLYVCLMSVNALEAIRFYVSFACSFAFAERELMEGNAKIIKLIARDEA
LHLTGTQHMINLLRNGQDDFEFMQIAEECKQECFDLFKEAAEQEKEWAEYLFKDGSMIGLNKDILCQYVEYITNIRMQAV
GLGTAYPEATSNPIPWINAWLSSDNVQVAPQEAEISSYLVGQIDNEVKADDFEGFEL
>Mature_376_residues
AYSTFSQQKNDQLKEPMFLGQSVNVARYDQQKFEIFEKLIEKQLSFFWRPEEVDVSSDRIDYNKLPEHEKHIFISNLKYQ
TLLDSIQGRSPNVALLPLVSLPEVETWIETWSFSETIHSRSYTHIIRNIVNDPGVVFDDIVENEEILKRAKDIAFYYDDL
IKLTADYHRYGEGNHNINGEDVKISLHDLKKKLYVCLMSVNALEAIRFYVSFACSFAFAERELMEGNAKIIKLIARDEAL
HLTGTQHMINLLRNGQDDFEFMQIAEECKQECFDLFKEAAEQEKEWAEYLFKDGSMIGLNKDILCQYVEYITNIRMQAVG
LGTAYPEATSNPIPWINAWLSSDNVQVAPQEAEISSYLVGQIDNEVKADDFEGFEL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2 contains the tyrosyl radical required for catalysis [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=313, Percent_Identity=25.2396166134185, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI289177074, Length=313, Percent_Identity=25.2396166134185, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788567, Length=377, Percent_Identity=80.3713527851459, Blast_Score=646, Evalue=0.0,
Organism=Escherichia coli, GI1789031, Length=321, Percent_Identity=25.8566978193146, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 43671; Mature: 43540

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00368 RIBORED_SMALL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYSTFSQQKNDQLKEPMFLGQSVNVARYDQQKFEIFEKLIEKQLSFFWRPEEVDVSSDR
CCCCCCCHHHHHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
IDYNKLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSLPEVETWIETWSFSETIHS
CCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
RSYTHIIRNIVNDPGVVFDDIVENEEILKRAKDIAFYYDDLIKLTADYHRYGEGNHNING
HHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EDVKISLHDLKKKLYVCLMSVNALEAIRFYVSFACSFAFAERELMEGNAKIIKLIARDEA
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCE
LHLTGTQHMINLLRNGQDDFEFMQIAEECKQECFDLFKEAAEQEKEWAEYLFKDGSMIGL
EEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECC
NKDILCQYVEYITNIRMQAVGLGTAYPEATSNPIPWINAWLSSDNVQVAPQEAEISSYLV
CHHHHHHHHHHHHCCHHHEEECCCCCCCCCCCCCCHHHHEECCCCCEECCCHHHHHHHHH
GQIDNEVKADDFEGFEL
HHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AYSTFSQQKNDQLKEPMFLGQSVNVARYDQQKFEIFEKLIEKQLSFFWRPEEVDVSSDR
CCCCCCHHHHHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
IDYNKLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSLPEVETWIETWSFSETIHS
CCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
RSYTHIIRNIVNDPGVVFDDIVENEEILKRAKDIAFYYDDLIKLTADYHRYGEGNHNING
HHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EDVKISLHDLKKKLYVCLMSVNALEAIRFYVSFACSFAFAERELMEGNAKIIKLIARDEA
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCE
LHLTGTQHMINLLRNGQDDFEFMQIAEECKQECFDLFKEAAEQEKEWAEYLFKDGSMIGL
EEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECC
NKDILCQYVEYITNIRMQAVGLGTAYPEATSNPIPWINAWLSSDNVQVAPQEAEISSYLV
CHHHHHHHHHHHHCCHHHEEECCCCCCCCCCCCCCHHHHEECCCCCEECCCHHHHHHHHH
GQIDNEVKADDFEGFEL
HHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]