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The map label for this gene is nrdA [H]

Identifier: 218709926

GI number: 218709926

Start: 2082979

End: 2085261

Strand: Direct

Name: nrdA [H]

Synonym: VS_1939

Alternate gene names: 218709926

Gene position: 2082979-2085261 (Clockwise)

Preceding gene: 218709924

Following gene: 218709927

Centisome position: 63.13

GC content: 45.12

Gene sequence:

>2283_bases
ATGAACCAACAACTTACTGTTACCAAACGTAACGGGCGCAAAGAAACAATCGATTTAGAGAAAATCCATCGAGTGATCAC
ATGGGCAGCTGAAGGCTTGCACAATGTTTCTGTATCACAAGTAGAATTGAAAGCTCACATTCAGTTTTACGATGGCATCA
CCACCACTGACATCCATGAGACAATCATCAAGTCAGCAGCGGATTTAATCTCTGAAGAGACTCCTGATTACCAATATCTA
GCAGCACGTCTGTCAGTATTCCACCTACGTAAAAAAGCGTACGGCGAGTACGAGCCACCGGCTCTATATGACCACGTTAC
AAAACTGGTTGATATGGGTAAATACGATAGCCACCTAATGGAAGACTACACGAAAGCAGAGTTTGACGAGCTTGACGCGT
ACATCGACCACAAGCGTGACTTGGACTTCTCTTATGCAGCGGTCAAGCAGCTTGAAGGTAAATACTTCGTGCAAAACCGT
GTAACAAAAGAAATCTACGAGAGTGCTCAGTTCCTTTACATCCTAGTTGCTGCTTGTTTATTCGGTAAATACCCGAAATC
AACTCGTCTGGATTACATCAAACGTTTTTACGACGCCTCGTCTACGTTTAAGATTTCTCTACCTACACCAATTATGTCAG
GTGTACGTACGCCTACTCGTCAATTCAGTTCTTGTGTACTGATCGAGTGTGGTGACAGCCTTGATTCTATCAACGCAACA
GCAAGCTCTATTGTTCGTTACGTATCTCAACGTGCTGGTATTGGTATCAACGCAGGTCGTATCCGTGCGCTGGGTTCTGA
AATTCGTGGTGGTGAAGCGTTCCACACTGGCTGTATCCCTTTCTACAAATACTTCCAAACAGCAGTAAAATGTTGTTCTC
AAGGTGGTGTTCGTGGCGGTGCAGCAACCGTGTTCTACCCACTATGGCACGGTGAAGTTCAGTCTCTATTAGTACTGAAA
AACAACCGTGGTGTTGAAGAGAACCGTGTTCGTCACATGGACTACGGCGTACAGCTTAATAAGCTTATGTACTCTCGTCT
TGTTCAAGGTGGCAACATCAGCCTGTTCTCCCCTTCAGATGTACCTGGACTTTACGATGCGTTCTTCGAAAACCAAGAGC
GTTTTGAAGAGCTGTACGTTAAGTACGAAAACGATCCTTCAGTGAAGCGTGAAACTGTGAAAGCGGTTGAACTGTTCTCT
CTACTAATGCAAGAGCGTGCTTCTACTGGTCGTATCTACATTCAGAACGTTGACCACTGTAATACACACAGCCCGTTTGA
TTCTGAAGTAGCACCTGTTCGTCAATCGAACCTATGTCTAGAAATCGCGCTACCAACTAAGCCACTTTCTAACGTGGAAG
ATGACGAAGGTGAGATTGCACTGTGTACGCTTTCAGCGTTCAACCTTGGCGCAATCAATGAACTCGACGACTTAGCAGAG
CTTTCTGAGTTGGTTGTTCGTGCACTTGATGCACTGCTTGATTACCAAGACTACCCGCTTCCAGCAGCATACAAATCGAC
AATGAATCGTCGTACTTTAGGTGTGGGTGTCATCAACTATGCATACTACCTGGCGAAGAATGGTGTTAAGTACTCTGACG
GCAGCGCAAATGGCCTGACTCACCGTACTTTTGAAGCAATTCAATACCACTTACTAAAAGCGTCTGTTGAACTAGCGAAA
GAACAAGGCCGTTGCCCATCTTTCCACGAGACCAACTACGCGAAAGGCCTACTGCCAATCGATACTTATAAAAAAGATAT
CGACTTAGTCTGTGAAGAGCCTCTGCACTACGATTGGGACACGCTACGTGAAGAGATCATGGAGCACGGTCTGCGTAACT
CTACGCTAACAGCGCTTATGCCTTCTGAGACTTCGTCTCAAATCTCGAACGCGACTAACGGTATCGAGCCACCACGTGGT
TATGTATCAGTGAAAGCGTCGAAAGACGGTATCCTGAAGCAAGTTGTTCCTGATTTCTTAAATCTTAAAAACAACTACGA
GCTACTTTGGAACATTGGCAATAACGACGGTTACCTACACTTAGTGGGTATCATGCAAAAATTCGTTGACCAAGCTATCT
CTGCAAACACTAACTATGATCCAAGTGTTTACGACAGCGGTAAGGTTCCAATGAAGCAGCTGCTTAAAGACCTACTAACT
GCATACAAGTATGGTGTTAAAACGCTTTACTACCATAACACGCGTGATGGTGCTAAAGACGACCAAGGCGATGCAGTTCA
AGTGCCGGAAGAAGATTGTGAAGGCGGCGGTTGTAAGATCTAA

Upstream 100 bases:

>100_bases
GGTAGACCAAGATCACAAAGCCGAAACGTAATTTACAAAAATTACACAGAAATACACAAAAACACGGCTTTGTTCAGTTT
TGTTAGGGAAATTAAGCAGA

Downstream 100 bases:

>100_bases
ACCGCAATAAACATTAAGACCCCAGAGAAGAGTTATCTTCTCTGGGTTTAAAGCATTAACAGGGTCCGTTTTATTGCGGG
TCTTTAAAGGATTTGAGGCA

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Protein B1; Ribonucleoside-diphosphate reductase 1 R1 subunit; Ribonucleotide reductase 1 [H]

Number of amino acids: Translated: 760; Mature: 760

Protein sequence:

>760_residues
MNQQLTVTKRNGRKETIDLEKIHRVITWAAEGLHNVSVSQVELKAHIQFYDGITTTDIHETIIKSAADLISEETPDYQYL
AARLSVFHLRKKAYGEYEPPALYDHVTKLVDMGKYDSHLMEDYTKAEFDELDAYIDHKRDLDFSYAAVKQLEGKYFVQNR
VTKEIYESAQFLYILVAACLFGKYPKSTRLDYIKRFYDASSTFKISLPTPIMSGVRTPTRQFSSCVLIECGDSLDSINAT
ASSIVRYVSQRAGIGINAGRIRALGSEIRGGEAFHTGCIPFYKYFQTAVKCCSQGGVRGGAATVFYPLWHGEVQSLLVLK
NNRGVEENRVRHMDYGVQLNKLMYSRLVQGGNISLFSPSDVPGLYDAFFENQERFEELYVKYENDPSVKRETVKAVELFS
LLMQERASTGRIYIQNVDHCNTHSPFDSEVAPVRQSNLCLEIALPTKPLSNVEDDEGEIALCTLSAFNLGAINELDDLAE
LSELVVRALDALLDYQDYPLPAAYKSTMNRRTLGVGVINYAYYLAKNGVKYSDGSANGLTHRTFEAIQYHLLKASVELAK
EQGRCPSFHETNYAKGLLPIDTYKKDIDLVCEEPLHYDWDTLREEIMEHGLRNSTLTALMPSETSSQISNATNGIEPPRG
YVSVKASKDGILKQVVPDFLNLKNNYELLWNIGNNDGYLHLVGIMQKFVDQAISANTNYDPSVYDSGKVPMKQLLKDLLT
AYKYGVKTLYYHNTRDGAKDDQGDAVQVPEEDCEGGGCKI

Sequences:

>Translated_760_residues
MNQQLTVTKRNGRKETIDLEKIHRVITWAAEGLHNVSVSQVELKAHIQFYDGITTTDIHETIIKSAADLISEETPDYQYL
AARLSVFHLRKKAYGEYEPPALYDHVTKLVDMGKYDSHLMEDYTKAEFDELDAYIDHKRDLDFSYAAVKQLEGKYFVQNR
VTKEIYESAQFLYILVAACLFGKYPKSTRLDYIKRFYDASSTFKISLPTPIMSGVRTPTRQFSSCVLIECGDSLDSINAT
ASSIVRYVSQRAGIGINAGRIRALGSEIRGGEAFHTGCIPFYKYFQTAVKCCSQGGVRGGAATVFYPLWHGEVQSLLVLK
NNRGVEENRVRHMDYGVQLNKLMYSRLVQGGNISLFSPSDVPGLYDAFFENQERFEELYVKYENDPSVKRETVKAVELFS
LLMQERASTGRIYIQNVDHCNTHSPFDSEVAPVRQSNLCLEIALPTKPLSNVEDDEGEIALCTLSAFNLGAINELDDLAE
LSELVVRALDALLDYQDYPLPAAYKSTMNRRTLGVGVINYAYYLAKNGVKYSDGSANGLTHRTFEAIQYHLLKASVELAK
EQGRCPSFHETNYAKGLLPIDTYKKDIDLVCEEPLHYDWDTLREEIMEHGLRNSTLTALMPSETSSQISNATNGIEPPRG
YVSVKASKDGILKQVVPDFLNLKNNYELLWNIGNNDGYLHLVGIMQKFVDQAISANTNYDPSVYDSGKVPMKQLLKDLLT
AYKYGVKTLYYHNTRDGAKDDQGDAVQVPEEDCEGGGCKI
>Mature_760_residues
MNQQLTVTKRNGRKETIDLEKIHRVITWAAEGLHNVSVSQVELKAHIQFYDGITTTDIHETIIKSAADLISEETPDYQYL
AARLSVFHLRKKAYGEYEPPALYDHVTKLVDMGKYDSHLMEDYTKAEFDELDAYIDHKRDLDFSYAAVKQLEGKYFVQNR
VTKEIYESAQFLYILVAACLFGKYPKSTRLDYIKRFYDASSTFKISLPTPIMSGVRTPTRQFSSCVLIECGDSLDSINAT
ASSIVRYVSQRAGIGINAGRIRALGSEIRGGEAFHTGCIPFYKYFQTAVKCCSQGGVRGGAATVFYPLWHGEVQSLLVLK
NNRGVEENRVRHMDYGVQLNKLMYSRLVQGGNISLFSPSDVPGLYDAFFENQERFEELYVKYENDPSVKRETVKAVELFS
LLMQERASTGRIYIQNVDHCNTHSPFDSEVAPVRQSNLCLEIALPTKPLSNVEDDEGEIALCTLSAFNLGAINELDDLAE
LSELVVRALDALLDYQDYPLPAAYKSTMNRRTLGVGVINYAYYLAKNGVKYSDGSANGLTHRTFEAIQYHLLKASVELAK
EQGRCPSFHETNYAKGLLPIDTYKKDIDLVCEEPLHYDWDTLREEIMEHGLRNSTLTALMPSETSSQISNATNGIEPPRG
YVSVKASKDGILKQVVPDFLNLKNNYELLWNIGNNDGYLHLVGIMQKFVDQAISANTNYDPSVYDSGKVPMKQLLKDLLT
AYKYGVKTLYYHNTRDGAKDDQGDAVQVPEEDCEGGGCKI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1 contains the binding sites for both substrates and allosteric effectors and carries out the actual reduction

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-cone domain [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=787, Percent_Identity=27.8271918678526, Blast_Score=249, Evalue=8e-66,
Organism=Escherichia coli, GI2367133, Length=761, Percent_Identity=79.5006570302234, Blast_Score=1292, Evalue=0.0,
Organism=Escherichia coli, GI1789030, Length=564, Percent_Identity=25.177304964539, Blast_Score=153, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17555242, Length=779, Percent_Identity=28.7548138639281, Blast_Score=251, Evalue=7e-67,
Organism=Saccharomyces cerevisiae, GI6322123, Length=657, Percent_Identity=28.9193302891933, Blast_Score=257, Evalue=4e-69,
Organism=Saccharomyces cerevisiae, GI6320914, Length=654, Percent_Identity=28.7461773700306, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI17352481, Length=650, Percent_Identity=31.0769230769231, Blast_Score=264, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005144
- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926 [H]

Pfam domain/function: PF03477 ATP-cone; PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 85538; Mature: 85538

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS51161 ATP_CONE ; PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQQLTVTKRNGRKETIDLEKIHRVITWAAEGLHNVSVSQVELKAHIQFYDGITTTDIHE
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHEEEEEEEEEEECCCCCHHHHH
TIIKSAADLISEETPDYQYLAARLSVFHLRKKAYGEYEPPALYDHVTKLVDMGKYDSHLM
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHH
EDYTKAEFDELDAYIDHKRDLDFSYAAVKQLEGKYFVQNRVTKEIYESAQFLYILVAACL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FGKYPKSTRLDYIKRFYDASSTFKISLPTPIMSGVRTPTRQFSSCVLIECGDSLDSINAT
HCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCHHHHCCEEEEEECCCHHHHHHH
ASSIVRYVSQRAGIGINAGRIRALGSEIRGGEAFHTGCIPFYKYFQTAVKCCSQGGVRGG
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHCCCCCCC
AATVFYPLWHGEVQSLLVLKNNRGVEENRVRHMDYGVQLNKLMYSRLVQGGNISLFSPSD
CEEEEEEECCCCHHEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCC
VPGLYDAFFENQERFEELYVKYENDPSVKRETVKAVELFSLLMQERASTGRIYIQNVDHC
CCHHHHHHHCCHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
NTHSPFDSEVAPVRQSNLCLEIALPTKPLSNVEDDEGEIALCTLSAFNLGAINELDDLAE
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHH
LSELVVRALDALLDYQDYPLPAAYKSTMNRRTLGVGVINYAYYLAKNGVKYSDGSANGLT
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCH
HRTFEAIQYHLLKASVELAKEQGRCPSFHETNYAKGLLPIDTYKKDIDLVCEEPLHYDWD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCHHHHCCCCCCCCHH
TLREEIMEHGLRNSTLTALMPSETSSQISNATNGIEPPRGYVSVKASKDGILKQVVPDFL
HHHHHHHHCCCCCCCEEEECCCCHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHH
NLKNNYELLWNIGNNDGYLHLVGIMQKFVDQAISANTNYDPSVYDSGKVPMKQLLKDLLT
CCCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
AYKYGVKTLYYHNTRDGAKDDQGDAVQVPEEDCEGGGCKI
HHHHCCCEEEEECCCCCCCCCCCCEEECCHHHCCCCCCCC
>Mature Secondary Structure
MNQQLTVTKRNGRKETIDLEKIHRVITWAAEGLHNVSVSQVELKAHIQFYDGITTTDIHE
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHEEEEEEEEEEECCCCCHHHHH
TIIKSAADLISEETPDYQYLAARLSVFHLRKKAYGEYEPPALYDHVTKLVDMGKYDSHLM
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHH
EDYTKAEFDELDAYIDHKRDLDFSYAAVKQLEGKYFVQNRVTKEIYESAQFLYILVAACL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FGKYPKSTRLDYIKRFYDASSTFKISLPTPIMSGVRTPTRQFSSCVLIECGDSLDSINAT
HCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCHHHHCCEEEEEECCCHHHHHHH
ASSIVRYVSQRAGIGINAGRIRALGSEIRGGEAFHTGCIPFYKYFQTAVKCCSQGGVRGG
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHCCCCCCC
AATVFYPLWHGEVQSLLVLKNNRGVEENRVRHMDYGVQLNKLMYSRLVQGGNISLFSPSD
CEEEEEEECCCCHHEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCC
VPGLYDAFFENQERFEELYVKYENDPSVKRETVKAVELFSLLMQERASTGRIYIQNVDHC
CCHHHHHHHCCHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
NTHSPFDSEVAPVRQSNLCLEIALPTKPLSNVEDDEGEIALCTLSAFNLGAINELDDLAE
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHH
LSELVVRALDALLDYQDYPLPAAYKSTMNRRTLGVGVINYAYYLAKNGVKYSDGSANGLT
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCH
HRTFEAIQYHLLKASVELAKEQGRCPSFHETNYAKGLLPIDTYKKDIDLVCEEPLHYDWD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCHHHHCCCCCCCCHH
TLREEIMEHGLRNSTLTALMPSETSSQISNATNGIEPPRGYVSVKASKDGILKQVVPDFL
HHHHHHHHCCCCCCCEEEECCCCHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHH
NLKNNYELLWNIGNNDGYLHLVGIMQKFVDQAISANTNYDPSVYDSGKVPMKQLLKDLLT
CCCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
AYKYGVKTLYYHNTRDGAKDDQGDAVQVPEEDCEGGGCKI
HHHHCCCEEEEECCCCCCCCCCCCEEECCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6087316; 3287341; 9205837; 9278503; 3894026; 2663852; 8702814; 8052308; 9309223; 10493864 [H]