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The map label for this gene is opuD [H]

Identifier: 218709896

GI number: 218709896

Start: 2051555

End: 2053153

Strand: Reverse

Name: opuD [H]

Synonym: VS_1909

Alternate gene names: 218709896

Gene position: 2053153-2051555 (Counterclockwise)

Preceding gene: 218709897

Following gene: 218709895

Centisome position: 62.23

GC content: 45.09

Gene sequence:

>1599_bases
ATGGAGCCTGTAAAGGATAGGTATAGTATTGATAATACTGATTATACCGTAGGACAAGATAATGTTCAGAAATGGGGTTT
TGATGTTCATAACCCTGTATTTGGAATCAGTGCTGGGGCGATAATTGCTTTCCTGATTGCGCTTTTGGTTGCAGAACCTG
AAACAGCTAAAGCGGCACTTGATGGCGTTAAGTGGAAAGTCATCGGTAACTTCGATGGTTTCTTTATGTGGGCTGCGAAC
CTTTTCGTATTTTTCTGTTTTGTCCTCATTATCTCCCCGTACGGAAAGATACGTATAGGTGGCGATGATGCTAAAGCAGA
GCACTCTAACCTATCTTGGATGTCGATGTTATTCGCCGCGGGAATGGGTATTGGTTTGATGTTCTGGGGTGTGGCAGAGC
CAGTAGCTTACTTTACCGGTTGGTATGAAACACCGCTCGGTGTTGAAGCGTATTCTCCTGAAGCGGCTAAGTTGGCACTC
GGTGCAACCATCTACAACTGGGGCTTGCACGGTTGGGCAATCTACGCCGTTGTTGCTTTGTCTCTTGCCTTCTTTACTTT
CAATAAAGGCTTACCGCTTTCTATTCGCTCAATCTTTTACCCGCTACTCGGTGACAGAACTTGGGGGTGGTTTGGTCATA
TCGTTGACATTGTCGCAGTACTTGCAACATTGTTTGGCCTTGCAACATCACTTGGCCTAGGTGCACAGCAAGCCGCAAGC
GGTATTAACCATGTATTTGGTACCGATGGCGGTATTGGTATGCAGCTTATTGTTATTGCAGTGGTAACACTGTTGGCAAC
AATGTCGGTGATTCGCGGTATTAACGGTGGTGTTAAGGTTCTCAGCAACGTCAACATGGTTGTTGCATTAGGCTTGCTGA
TCTTCGTAACTATCGCGGGTGGCATGGCGGGTATTAAGGCGATACCAACCGCGCTAATGGGCTACATTGAGAACTTTATT
CCTCTTAGCAACCCTCATGGTCGTGATGATGAAACATGGATGCAAGGTTGGACGGTATTTTACTGGGCTTGGTGGATTTC
ATGGTCACCATTCGTAGGCATGTTCATCGCTCGTATCTCTAAAGGTCGTACAATTCGTGAATTCATCGTTGCAGTCTTAT
TTATCCCGACGTCTGTCATTATCATTTGGATGGCTATTTTCGGTGGTATTGCGATTGACCAGGTGGCGAATAAAGTGGGT
GAGATTGGTATAAATGGTCTGCAAGATATTACACTTTCTCTATTCCATACTTACGATGCGTTGCCAATGAGCTCAGTCCT
TTCCGTGGTATCGATTGGTTTGATTATGGTGTTTTTCATCACTTCATCAGACTCAGGTTCATTGGTTATTGATAGCATTA
CTTCTGGCGGAAAAGTCGATGCACCTGTTCCACAGCGTGTATTTTGGGCATTGATGGGCGGCGCGATTGCGGCGGTCCTA
CTGTGGGTTGGTGGTACTGAATCTATTCAGGCATTACAAGCTGGAACAGTCTCAATGGCATTACCATTTGCCTTCATATT
ATTGCTTATGTGCCTAAGTTTGTTTCTTGGTTTACGTACAGAGAATCAATTGGTTAAGCAGCAAAGTGCTTTGAGCTAA

Upstream 100 bases:

>100_bases
GTTTTTTTAGTATTTTTGCGACGAGTTGTTATTCTTCCTTTCGAAATTTAAGTGCCAAATGCAATGTTGCAAATTGTGCG
CTACTAATTAGAGAGGATGC

Downstream 100 bases:

>100_bases
AAGTATTTTTTCAAATAGAGGCCGACTTAGTCGGCCTTTTTTTCGCCGCAAACATAAGTGATTACCTATATTTTGTTTGA
AAATCCATCGAACTGATTCT

Product: BCCT family transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MEPVKDRYSIDNTDYTVGQDNVQKWGFDVHNPVFGISAGAIIAFLIALLVAEPETAKAALDGVKWKVIGNFDGFFMWAAN
LFVFFCFVLIISPYGKIRIGGDDAKAEHSNLSWMSMLFAAGMGIGLMFWGVAEPVAYFTGWYETPLGVEAYSPEAAKLAL
GATIYNWGLHGWAIYAVVALSLAFFTFNKGLPLSIRSIFYPLLGDRTWGWFGHIVDIVAVLATLFGLATSLGLGAQQAAS
GINHVFGTDGGIGMQLIVIAVVTLLATMSVIRGINGGVKVLSNVNMVVALGLLIFVTIAGGMAGIKAIPTALMGYIENFI
PLSNPHGRDDETWMQGWTVFYWAWWISWSPFVGMFIARISKGRTIREFIVAVLFIPTSVIIIWMAIFGGIAIDQVANKVG
EIGINGLQDITLSLFHTYDALPMSSVLSVVSIGLIMVFFITSSDSGSLVIDSITSGGKVDAPVPQRVFWALMGGAIAAVL
LWVGGTESIQALQAGTVSMALPFAFILLLMCLSLFLGLRTENQLVKQQSALS

Sequences:

>Translated_532_residues
MEPVKDRYSIDNTDYTVGQDNVQKWGFDVHNPVFGISAGAIIAFLIALLVAEPETAKAALDGVKWKVIGNFDGFFMWAAN
LFVFFCFVLIISPYGKIRIGGDDAKAEHSNLSWMSMLFAAGMGIGLMFWGVAEPVAYFTGWYETPLGVEAYSPEAAKLAL
GATIYNWGLHGWAIYAVVALSLAFFTFNKGLPLSIRSIFYPLLGDRTWGWFGHIVDIVAVLATLFGLATSLGLGAQQAAS
GINHVFGTDGGIGMQLIVIAVVTLLATMSVIRGINGGVKVLSNVNMVVALGLLIFVTIAGGMAGIKAIPTALMGYIENFI
PLSNPHGRDDETWMQGWTVFYWAWWISWSPFVGMFIARISKGRTIREFIVAVLFIPTSVIIIWMAIFGGIAIDQVANKVG
EIGINGLQDITLSLFHTYDALPMSSVLSVVSIGLIMVFFITSSDSGSLVIDSITSGGKVDAPVPQRVFWALMGGAIAAVL
LWVGGTESIQALQAGTVSMALPFAFILLLMCLSLFLGLRTENQLVKQQSALS
>Mature_532_residues
MEPVKDRYSIDNTDYTVGQDNVQKWGFDVHNPVFGISAGAIIAFLIALLVAEPETAKAALDGVKWKVIGNFDGFFMWAAN
LFVFFCFVLIISPYGKIRIGGDDAKAEHSNLSWMSMLFAAGMGIGLMFWGVAEPVAYFTGWYETPLGVEAYSPEAAKLAL
GATIYNWGLHGWAIYAVVALSLAFFTFNKGLPLSIRSIFYPLLGDRTWGWFGHIVDIVAVLATLFGLATSLGLGAQQAAS
GINHVFGTDGGIGMQLIVIAVVTLLATMSVIRGINGGVKVLSNVNMVVALGLLIFVTIAGGMAGIKAIPTALMGYIENFI
PLSNPHGRDDETWMQGWTVFYWAWWISWSPFVGMFIARISKGRTIREFIVAVLFIPTSVIIIWMAIFGGIAIDQVANKVG
EIGINGLQDITLSLFHTYDALPMSSVLSVVSIGLIMVFFITSSDSGSLVIDSITSGGKVDAPVPQRVFWALMGGAIAAVL
LWVGGTESIQALQAGTVSMALPFAFILLLMCLSLFLGLRTENQLVKQQSALS

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=513, Percent_Identity=37.037037037037, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1786224, Length=485, Percent_Identity=27.8350515463918, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1788102, Length=438, Percent_Identity=29.6803652968037, Blast_Score=162, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 57318; Mature: 57318

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure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HHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]