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The map label for this gene is opuD [H]

Identifier: 218709895

GI number: 218709895

Start: 2049711

End: 2051282

Strand: Reverse

Name: opuD [H]

Synonym: VS_1908

Alternate gene names: 218709895

Gene position: 2051282-2049711 (Counterclockwise)

Preceding gene: 218709896

Following gene: 218709894

Centisome position: 62.17

GC content: 44.85

Gene sequence:

>1572_bases
ATGACTAAAGGTATAGATAAATACAGTATCGACAGTACGGATTACACTGTTGGTCAAGATAACGTCCAAAAATGGGGTTT
TGACGTTCATAACCCAGTGTTTGGTATCAGTGCGGGACTCATCACTTTGTTCTTGATCGGTGTGCTGCTTACAGATACCG
CTTCAGCGAAGTCAGCATTGGATGGTGTTAAATGGCAAATTATCAATTCATTTGATTGGTTGTTCATTTGGTCCGGTAAT
ATTTTCGTTATTTTTTGCTTAGGCTTGATTGTTTCTCCGTTTGGTAAAATCCGCCTTGGTGGTGTTGATGCAACGGCAGA
TTACTCATTCATGTCTTGGTTATCGATGCTGTTTGCTGCAGGTATGGGTATTGGCTTGATGTTCTGGAGTGTTGCAGAAC
CTGCGGCTTACTACACAGGTTGGTATGAAACGCCACTTGGCGTAGAGGCAAATACACCAGAAGCTGCAAAACTAGCACTC
GGTGCAACCATGTATCACTGGGGGCTTCACCCTTGGGCTATTTATGGTGTTGTGGCGCTTTCACTGGCTTTCTTCTCTTA
CAACAAGGGATTACCGCTTTCAATTCGTTCTATCTTCTACCCAATTTTAGGTGATAGAGCGTGGGGCTGGGCTGGTCACA
TCGTTGATATTCTTGCTGTACTTGCAACCCTGTTTGGTTTGGCGACATCGCTTGGCCTTGGTGCGCAACAAGCGGCAAGT
GGTATTCAGCATGTATTCGGCATTGAGGCAGGCTTAGGGTTACAAGTCATTGTTATTACCGTAGTAACTTTGCTAGCGGT
CGTATCCGTACTTCGTGGTATTGATGGTGGCGTTAAAGTTATCAGTAACATCAACATGTTGGTTGCTTTCCTACTGCTTA
TCCTAGTAGCTCTAATCGGCTACGCAGTGACTCTAGGTTCAATCCCTACAACATTGATGGCATACATCGAGAACATCATT
CCGTTGAGCAACCCTCACGGTCGTGAAGATGAAGCTTGGTTACATGGTTGGACTGTATTCTACTGGGCCTGGTGGATTTC
ATGGTCTCCATTTGTCGGTATGTTTATCGCGCGTGTTTCTAAAGGTCGTACTGTTCGTGAGTTCATTACAGCGGTACTGA
TTGTTCCAACGACAGTGACTATCATTTGGATGTCAGTGTTCGGTGGTATGGCAATTGATCAAATCGTGAATAAGGTTGGT
ACCCTAGGTCAAAATGGCTTAACTGATGTATCACTGGCAATGTTCCAAATGTTTGATGTATTGCCGTTTGGTACTCTGCT
TTCAATCATTGCGGTTGTATTGGTTCTCGTATTCTTTATTACATCGTCTGACTCGGGCTCTTTAGTTATCGACAGCATTA
CTGCTGGTGGTAAAGTTGATACGCCTGTGCCTCAACGTGTGTTCTGGGCGTTCCTTGAAGGTGCGATTGCAGTAGCTCTA
TTGTGGGTTGGCGGTACTGAAGCCGTTCAAGCTCTGCAAGCGGGTGCAATCTCAACCGCATTGCCATTCACCATTATCCT
ATTATTGATGTGTGTGAGCCTACTAATGGGTATGCGAACAGAGAAAAGATAG

Upstream 100 bases:

>100_bases
TTGTACTACTTGGATCATATGTTCGAGTTTGTGATTCAGTCACAGGAAAGTAAGGGATATGAGGTCAACAGACCTTATAA
GTATTCCGTAGAGAGGAATA

Downstream 100 bases:

>100_bases
ACTAGATACTATTCGATTTTTCATTTTTTGATGTTGAATACAAAGTAAAACAAAAGGCAGAAGAGGAAACTCTCCTGCCT
TTTTTGTATCCGTTTTACAG

Product: BCCT family transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MTKGIDKYSIDSTDYTVGQDNVQKWGFDVHNPVFGISAGLITLFLIGVLLTDTASAKSALDGVKWQIINSFDWLFIWSGN
IFVIFCLGLIVSPFGKIRLGGVDATADYSFMSWLSMLFAAGMGIGLMFWSVAEPAAYYTGWYETPLGVEANTPEAAKLAL
GATMYHWGLHPWAIYGVVALSLAFFSYNKGLPLSIRSIFYPILGDRAWGWAGHIVDILAVLATLFGLATSLGLGAQQAAS
GIQHVFGIEAGLGLQVIVITVVTLLAVVSVLRGIDGGVKVISNINMLVAFLLLILVALIGYAVTLGSIPTTLMAYIENII
PLSNPHGREDEAWLHGWTVFYWAWWISWSPFVGMFIARVSKGRTVREFITAVLIVPTTVTIIWMSVFGGMAIDQIVNKVG
TLGQNGLTDVSLAMFQMFDVLPFGTLLSIIAVVLVLVFFITSSDSGSLVIDSITAGGKVDTPVPQRVFWAFLEGAIAVAL
LWVGGTEAVQALQAGAISTALPFTIILLLMCVSLLMGMRTEKR

Sequences:

>Translated_523_residues
MTKGIDKYSIDSTDYTVGQDNVQKWGFDVHNPVFGISAGLITLFLIGVLLTDTASAKSALDGVKWQIINSFDWLFIWSGN
IFVIFCLGLIVSPFGKIRLGGVDATADYSFMSWLSMLFAAGMGIGLMFWSVAEPAAYYTGWYETPLGVEANTPEAAKLAL
GATMYHWGLHPWAIYGVVALSLAFFSYNKGLPLSIRSIFYPILGDRAWGWAGHIVDILAVLATLFGLATSLGLGAQQAAS
GIQHVFGIEAGLGLQVIVITVVTLLAVVSVLRGIDGGVKVISNINMLVAFLLLILVALIGYAVTLGSIPTTLMAYIENII
PLSNPHGREDEAWLHGWTVFYWAWWISWSPFVGMFIARVSKGRTVREFITAVLIVPTTVTIIWMSVFGGMAIDQIVNKVG
TLGQNGLTDVSLAMFQMFDVLPFGTLLSIIAVVLVLVFFITSSDSGSLVIDSITAGGKVDTPVPQRVFWAFLEGAIAVAL
LWVGGTEAVQALQAGAISTALPFTIILLLMCVSLLMGMRTEKR
>Mature_522_residues
TKGIDKYSIDSTDYTVGQDNVQKWGFDVHNPVFGISAGLITLFLIGVLLTDTASAKSALDGVKWQIINSFDWLFIWSGNI
FVIFCLGLIVSPFGKIRLGGVDATADYSFMSWLSMLFAAGMGIGLMFWSVAEPAAYYTGWYETPLGVEANTPEAAKLALG
ATMYHWGLHPWAIYGVVALSLAFFSYNKGLPLSIRSIFYPILGDRAWGWAGHIVDILAVLATLFGLATSLGLGAQQAASG
IQHVFGIEAGLGLQVIVITVVTLLAVVSVLRGIDGGVKVISNINMLVAFLLLILVALIGYAVTLGSIPTTLMAYIENIIP
LSNPHGREDEAWLHGWTVFYWAWWISWSPFVGMFIARVSKGRTVREFITAVLIVPTTVTIIWMSVFGGMAIDQIVNKVGT
LGQNGLTDVSLAMFQMFDVLPFGTLLSIIAVVLVLVFFITSSDSGSLVIDSITAGGKVDTPVPQRVFWAFLEGAIAVALL
WVGGTEAVQALQAGAISTALPFTIILLLMCVSLLMGMRTEKR

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=507, Percent_Identity=34.7140039447732, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1786224, Length=465, Percent_Identity=28.8172043010753, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1788102, Length=445, Percent_Identity=29.8876404494382, Blast_Score=167, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 56415; Mature: 56284

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS50267 NA_NEUROTRAN_SYMP_3 ; PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKGIDKYSIDSTDYTVGQDNVQKWGFDVHNPVFGISAGLITLFLIGVLLTDTASAKSAL
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
DGVKWQIINSFDWLFIWSGNIFVIFCLGLIVSPFGKIRLGGVDATADYSFMSWLSMLFAA
CCCCEEEECCCCEEEEECCCHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHC
GMGIGLMFWSVAEPAAYYTGWYETPLGVEANTPEAAKLALGATMYHWGLHPWAIYGVVAL
CCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
SLAFFSYNKGLPLSIRSIFYPILGDRAWGWAGHIVDILAVLATLFGLATSLGLGAQQAAS
HHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GIQHVFGIEAGLGLQVIVITVVTLLAVVSVLRGIDGGVKVISNINMLVAFLLLILVALIG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YAVTLGSIPTTLMAYIENIIPLSNPHGREDEAWLHGWTVFYWAWWISWSPFVGMFIARVS
HHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGRTVREFITAVLIVPTTVTIIWMSVFGGMAIDQIVNKVGTLGQNGLTDVSLAMFQMFDV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LPFGTLLSIIAVVLVLVFFITSSDSGSLVIDSITAGGKVDTPVPQRVFWAFLEGAIAVAL
CCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
LWVGGTEAVQALQAGAISTALPFTIILLLMCVSLLMGMRTEKR
HHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TKGIDKYSIDSTDYTVGQDNVQKWGFDVHNPVFGISAGLITLFLIGVLLTDTASAKSAL
CCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
DGVKWQIINSFDWLFIWSGNIFVIFCLGLIVSPFGKIRLGGVDATADYSFMSWLSMLFAA
CCCCEEEECCCCEEEEECCCHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHC
GMGIGLMFWSVAEPAAYYTGWYETPLGVEANTPEAAKLALGATMYHWGLHPWAIYGVVAL
CCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
SLAFFSYNKGLPLSIRSIFYPILGDRAWGWAGHIVDILAVLATLFGLATSLGLGAQQAAS
HHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GIQHVFGIEAGLGLQVIVITVVTLLAVVSVLRGIDGGVKVISNINMLVAFLLLILVALIG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YAVTLGSIPTTLMAYIENIIPLSNPHGREDEAWLHGWTVFYWAWWISWSPFVGMFIARVS
HHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGRTVREFITAVLIVPTTVTIIWMSVFGGMAIDQIVNKVGTLGQNGLTDVSLAMFQMFDV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LPFGTLLSIIAVVLVLVFFITSSDSGSLVIDSITAGGKVDTPVPQRVFWAFLEGAIAVAL
CCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
LWVGGTEAVQALQAGAISTALPFTIILLLMCVSLLMGMRTEKR
HHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]