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The map label for this gene is fixL [H]

Identifier: 218709814

GI number: 218709814

Start: 1966205

End: 1968148

Strand: Direct

Name: fixL [H]

Synonym: VS_1826

Alternate gene names: 218709814

Gene position: 1966205-1968148 (Clockwise)

Preceding gene: 218709810

Following gene: 218709815

Centisome position: 59.59

GC content: 44.7

Gene sequence:

>1944_bases
GTGAATCAATACGCTGTTATCTGTTTGGACAATAATCCGGTTAGCATTGAAAGAATACGTTCGGAGTTGGCTCCGTTGGC
TTCTGTATTCGATATTTATACTGCCGAGAACATAGAAGACGCGCATCACGCATTAGAAGATATTCATGATCACAACCAAA
CCGTAGCCTTGGTGATCACTCATCATCATTCTGAATTTAATGGCGTGCAATTTCTGATTGAACTCGAACAGCTGCCCCAT
AGTAATACCGCAAGAACGATATTAGTCAGCGCTTCGTCAGACATTCAATCCATTCTAACCGCTGTAAATGAAGGCAGGCT
CAACCACTGTCTAACCAAGCCAGTTCAAGATCAGGTTCTATTCAAATCAGCGCAAAAAGAGCTAACGTCTTTTGTCATCC
AATACGACTCAGAAAACCTGCTCTCTTACAGCGATGCATTGGATCAACAGCGCTTACTCAGAGCGCACATTGAACAAAAG
ATTCACTCTTTCCAATCGGGTTTCATTCACGATTACCACCAGCTTTCTGACAATGCCCTTGCAGAACGCGTCGTCAGTGC
TCTGCAGGATGTCTTTTCAAAAGATGATAAAACCAAGGCCATTCGTGATTATTCACCAGAGCATCTGCTGACGGTTGAGG
GCGAAGATAATCGCTTTCTTTGGTTAATTATTGAAGGTGAAGCGGCGCTCTATAAAAAAGATGAACTGGGGCAGCAACGC
GAAGTTGTGCGTCACTCTAAGGGCAATATTGTCGGAGGGATGTCATTCGTGACGGGTGAGCCTTCGTTTTCTACTGCGAT
TACTCTGACTCAGACCCAAGTCATCAAACTGGATAAAGATAGCTTTGCTCAGGTGATGCATTCAAACAACACTCTGCTAC
CGCTGTTTACCAATCTACTACTCCGTCACTTTAACCGACGATTACAAAGAAGCATCAACAATAAGATCCAGCTTCAACAA
ACGCTAGAATCGTTGGAATCTGCACATCAACAATTGATAGAGAAAGAGAAGATGGCGATGCTCGGCCAACTCGTGGCGGG
TGTTGCACATGAGTTGAACAACCCGATTGCAGCGATCTTGCGAAGCATTGAAACCTTGTCTGAACATCTCGATCAAATAC
TCGAGAACTCGGCACTCCCAGAATCCAATAAAGGGACGGATGTATTAGCACAGTCAAAAATGGCGAAGCCACTATCAACG
GCTCAAGAAAGACAACTCGTAAAGCACCTCACCTCTACTATCGATGATCGTGCGCTAGCCAAAAAAGCCGTAAGACTGAA
CCTGAGCCAAGACTCTGCGGTTTTAGATACCTTAAAAGACTCCCCTGTCGCAGGCAAAGAGCTGCTCAATGACTTAGAGC
ATTATCACTATGTTGGAAACTCTATCCGCTCAATTCAAGTTTGCAGCAAACGTATTGCCGATATGGTCAAAAGCCTAAAA
AGCTATGCCCGAGAAGATGAAGAGGTCCGCCACTACACAGATATCCACGAAGGGCTTGAAGATACGTTAGTGATCTTTGA
AAACAGGCTCAAACATCACCAACTTGAAAAACACTACGACACCGACTTGCCGCTTCTATTGTGCCAGTCGCTCTCGCTGC
AACAAGTGTGGACCAACCTTCTCTCTAATGCACTTGATGCTCTTTCAGAGCGAGGCAAAGTCTCTATCACCACGTCTCAA
GAAACTAAAGGTGACGACACGTTTCTTGTAGTGCAAATATCGGACACTGGACACGGTATCGCCAAAGAAGACATCAACAC
TATTTTCAACCCAAACTTCACGACCAAAAAAGAAGGCAACTTCGGTTTAGGGATTGGGTTATCCATTTCTCAACAAATCG
TTTCGGCTCATCAAGGTTTTATTTTGGTGGAGTCTGAGGTAGGCAGCCATACACACATGCAAGTGTGGCTTCCATTCAAA
CAAGAAGGAGCACCTCATGAATAA

Upstream 100 bases:

>100_bases
GCTCGATACTTCGATTGCCTCAATAAATTAACGGCTAGCTCTCATCCTTATTCGGCGGTTCTTGCTAGGCTAGAGACGTC
GCTACTATTAAGGTTGAGCT

Downstream 100 bases:

>100_bases
ATACCTAATTTTATGTGTCGATGATGAACCTGAAGTTCTCAATAGTGTCCTTCAAGATTTAGCACCATTTGAAGACAACT
TTATTGTTGAAGGTGCCGAA

Product: putative signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MNQYAVICLDNNPVSIERIRSELAPLASVFDIYTAENIEDAHHALEDIHDHNQTVALVITHHHSEFNGVQFLIELEQLPH
SNTARTILVSASSDIQSILTAVNEGRLNHCLTKPVQDQVLFKSAQKELTSFVIQYDSENLLSYSDALDQQRLLRAHIEQK
IHSFQSGFIHDYHQLSDNALAERVVSALQDVFSKDDKTKAIRDYSPEHLLTVEGEDNRFLWLIIEGEAALYKKDELGQQR
EVVRHSKGNIVGGMSFVTGEPSFSTAITLTQTQVIKLDKDSFAQVMHSNNTLLPLFTNLLLRHFNRRLQRSINNKIQLQQ
TLESLESAHQQLIEKEKMAMLGQLVAGVAHELNNPIAAILRSIETLSEHLDQILENSALPESNKGTDVLAQSKMAKPLST
AQERQLVKHLTSTIDDRALAKKAVRLNLSQDSAVLDTLKDSPVAGKELLNDLEHYHYVGNSIRSIQVCSKRIADMVKSLK
SYAREDEEVRHYTDIHEGLEDTLVIFENRLKHHQLEKHYDTDLPLLLCQSLSLQQVWTNLLSNALDALSERGKVSITTSQ
ETKGDDTFLVVQISDTGHGIAKEDINTIFNPNFTTKKEGNFGLGIGLSISQQIVSAHQGFILVESEVGSHTHMQVWLPFK
QEGAPHE

Sequences:

>Translated_647_residues
MNQYAVICLDNNPVSIERIRSELAPLASVFDIYTAENIEDAHHALEDIHDHNQTVALVITHHHSEFNGVQFLIELEQLPH
SNTARTILVSASSDIQSILTAVNEGRLNHCLTKPVQDQVLFKSAQKELTSFVIQYDSENLLSYSDALDQQRLLRAHIEQK
IHSFQSGFIHDYHQLSDNALAERVVSALQDVFSKDDKTKAIRDYSPEHLLTVEGEDNRFLWLIIEGEAALYKKDELGQQR
EVVRHSKGNIVGGMSFVTGEPSFSTAITLTQTQVIKLDKDSFAQVMHSNNTLLPLFTNLLLRHFNRRLQRSINNKIQLQQ
TLESLESAHQQLIEKEKMAMLGQLVAGVAHELNNPIAAILRSIETLSEHLDQILENSALPESNKGTDVLAQSKMAKPLST
AQERQLVKHLTSTIDDRALAKKAVRLNLSQDSAVLDTLKDSPVAGKELLNDLEHYHYVGNSIRSIQVCSKRIADMVKSLK
SYAREDEEVRHYTDIHEGLEDTLVIFENRLKHHQLEKHYDTDLPLLLCQSLSLQQVWTNLLSNALDALSERGKVSITTSQ
ETKGDDTFLVVQISDTGHGIAKEDINTIFNPNFTTKKEGNFGLGIGLSISQQIVSAHQGFILVESEVGSHTHMQVWLPFK
QEGAPHE
>Mature_647_residues
MNQYAVICLDNNPVSIERIRSELAPLASVFDIYTAENIEDAHHALEDIHDHNQTVALVITHHHSEFNGVQFLIELEQLPH
SNTARTILVSASSDIQSILTAVNEGRLNHCLTKPVQDQVLFKSAQKELTSFVIQYDSENLLSYSDALDQQRLLRAHIEQK
IHSFQSGFIHDYHQLSDNALAERVVSALQDVFSKDDKTKAIRDYSPEHLLTVEGEDNRFLWLIIEGEAALYKKDELGQQR
EVVRHSKGNIVGGMSFVTGEPSFSTAITLTQTQVIKLDKDSFAQVMHSNNTLLPLFTNLLLRHFNRRLQRSINNKIQLQQ
TLESLESAHQQLIEKEKMAMLGQLVAGVAHELNNPIAAILRSIETLSEHLDQILENSALPESNKGTDVLAQSKMAKPLST
AQERQLVKHLTSTIDDRALAKKAVRLNLSQDSAVLDTLKDSPVAGKELLNDLEHYHYVGNSIRSIQVCSKRIADMVKSLK
SYAREDEEVRHYTDIHEGLEDTLVIFENRLKHHQLEKHYDTDLPLLLCQSLSLQQVWTNLLSNALDALSERGKVSITTSQ
ETKGDDTFLVVQISDTGHGIAKEDINTIFNPNFTTKKEGNFGLGIGLSISQQIVSAHQGFILVESEVGSHTHMQVWLPFK
QEGAPHE

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=125, Percent_Identity=36, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788549, Length=196, Percent_Identity=30.1020408163265, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 72733; Mature: 72733

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS50042 CNMP_BINDING_3 ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQYAVICLDNNPVSIERIRSELAPLASVFDIYTAENIEDAHHALEDIHDHNQTVALVIT
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEE
HHHSEFNGVQFLIELEQLPHSNTARTILVSASSDIQSILTAVNEGRLNHCLTKPVQDQVL
ECCCCCCHHHHEEEHHHCCCCCCCCEEEEECCHHHHHHHHHHHCCHHHHHHCCCHHHHHH
FKSAQKELTSFVIQYDSENLLSYSDALDQQRLLRAHIEQKIHSFQSGFIHDYHQLSDNAL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
AERVVSALQDVFSKDDKTKAIRDYSPEHLLTVEGEDNRFLWLIIEGEAALYKKDELGQQR
HHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEECCCCEEEEEEEECCHHHHCCHHCCHHH
EVVRHSKGNIVGGMSFVTGEPSFSTAITLTQTQVIKLDKDSFAQVMHSNNTLLPLFTNLL
HHHHHCCCCEEECCEEECCCCCCCEEEEEEEEEEEEECHHHHHHHHHCCCCCHHHHHHHH
LRHFNRRLQRSINNKIQLQQTLESLESAHQQLIEKEKMAMLGQLVAGVAHELNNPIAAIL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
RSIETLSEHLDQILENSALPESNKGTDVLAQSKMAKPLSTAQERQLVKHLTSTIDDRALA
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
KKAVRLNLSQDSAVLDTLKDSPVAGKELLNDLEHYHYVGNSIRSIQVCSKRIADMVKSLK
HHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SYAREDEEVRHYTDIHEGLEDTLVIFENRLKHHQLEKHYDTDLPLLLCQSLSLQQVWTNL
HHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHH
LSNALDALSERGKVSITTSQETKGDDTFLVVQISDTGHGIAKEDINTIFNPNFTTKKEGN
HHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCC
FGLGIGLSISQQIVSAHQGFILVESEVGSHTHMQVWLPFKQEGAPHE
CCEEECCHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEECCCCCCCC
>Mature Secondary Structure
MNQYAVICLDNNPVSIERIRSELAPLASVFDIYTAENIEDAHHALEDIHDHNQTVALVIT
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEE
HHHSEFNGVQFLIELEQLPHSNTARTILVSASSDIQSILTAVNEGRLNHCLTKPVQDQVL
ECCCCCCHHHHEEEHHHCCCCCCCCEEEEECCHHHHHHHHHHHCCHHHHHHCCCHHHHHH
FKSAQKELTSFVIQYDSENLLSYSDALDQQRLLRAHIEQKIHSFQSGFIHDYHQLSDNAL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
AERVVSALQDVFSKDDKTKAIRDYSPEHLLTVEGEDNRFLWLIIEGEAALYKKDELGQQR
HHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEECCCCEEEEEEEECCHHHHCCHHCCHHH
EVVRHSKGNIVGGMSFVTGEPSFSTAITLTQTQVIKLDKDSFAQVMHSNNTLLPLFTNLL
HHHHHCCCCEEECCEEECCCCCCCEEEEEEEEEEEEECHHHHHHHHHCCCCCHHHHHHHH
LRHFNRRLQRSINNKIQLQQTLESLESAHQQLIEKEKMAMLGQLVAGVAHELNNPIAAIL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
RSIETLSEHLDQILENSALPESNKGTDVLAQSKMAKPLSTAQERQLVKHLTSTIDDRALA
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
KKAVRLNLSQDSAVLDTLKDSPVAGKELLNDLEHYHYVGNSIRSIQVCSKRIADMVKSLK
HHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SYAREDEEVRHYTDIHEGLEDTLVIFENRLKHHQLEKHYDTDLPLLLCQSLSLQQVWTNL
HHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHH
LSNALDALSERGKVSITTSQETKGDDTFLVVQISDTGHGIAKEDINTIFNPNFTTKKEGN
HHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCC
FGLGIGLSISQQIVSAHQGFILVESEVGSHTHMQVWLPFKQEGAPHE
CCEEECCHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2046550; 1766374 [H]