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The map label for this gene is hupR1 [H]

Identifier: 218709815

GI number: 218709815

Start: 1968141

End: 1968638

Strand: Direct

Name: hupR1 [H]

Synonym: VS_1827

Alternate gene names: 218709815

Gene position: 1968141-1968638 (Clockwise)

Preceding gene: 218709814

Following gene: 218709816

Centisome position: 59.65

GC content: 41.77

Gene sequence:

>498_bases
ATGAATAAATACCTAATTTTATGTGTCGATGATGAACCTGAAGTTCTCAATAGTGTCCTTCAAGATTTAGCACCATTTGA
AGACAACTTTATTGTTGAAGGTGCCGAATCGGTCGATGAAGCTAAGCAAGTCGTTCAAGAAATGGGACAGGAAGGTATTA
AGCTCGCCTTAATCTTGTGTGACCACATCATGCCTGACAAAACGGGTATCGATTTTCTTATCGAACTTAACCAGCACGAC
TCTACAAAGCCAACACGCAAGCTGCTGCTCACCGGGCAAGCTGGGCTAGAAGATACCGTCACAGCAATTAACAACGCGGC
GCTTGATTTTTATATTTCAAAACCTTGGCAAGGCGATCAACTTAGAGAGACGATTACTCAACAACTGACAGACTATGTCA
TTGCAAACGACAAGCAGTTGTTAAACTGGACTTCCATTTTAGATACTGAGCGAATACTGACATCAATGTCAGACAAGCGA
ACTAGTTTCAGTGAATAG

Upstream 100 bases:

>100_bases
ATCGTTTCGGCTCATCAAGGTTTTATTTTGGTGGAGTCTGAGGTAGGCAGCCATACACACATGCAAGTGTGGCTTCCATT
CAAACAAGAAGGAGCACCTC

Downstream 100 bases:

>100_bases
CGATAGGTTGGTAAATAGAGGTTGTTTGCTGAATAGTGATTAGATGATGGATAGCGATTAGTTAGTCGCGATGCATCTTT
GACACTAAGTGTATTTTGTA

Product: putative response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MNKYLILCVDDEPEVLNSVLQDLAPFEDNFIVEGAESVDEAKQVVQEMGQEGIKLALILCDHIMPDKTGIDFLIELNQHD
STKPTRKLLLTGQAGLEDTVTAINNAALDFYISKPWQGDQLRETITQQLTDYVIANDKQLLNWTSILDTERILTSMSDKR
TSFSE

Sequences:

>Translated_165_residues
MNKYLILCVDDEPEVLNSVLQDLAPFEDNFIVEGAESVDEAKQVVQEMGQEGIKLALILCDHIMPDKTGIDFLIELNQHD
STKPTRKLLLTGQAGLEDTVTAINNAALDFYISKPWQGDQLRETITQQLTDYVIANDKQLLNWTSILDTERILTSMSDKR
TSFSE
>Mature_165_residues
MNKYLILCVDDEPEVLNSVLQDLAPFEDNFIVEGAESVDEAKQVVQEMGQEGIKLALILCDHIMPDKTGIDFLIELNQHD
STKPTRKLLLTGQAGLEDTVTAINNAALDFYISKPWQGDQLRETITQQLTDYVIANDKQLLNWTSILDTERILTSMSDKR
TSFSE

Specific function: Probable member of the two-component regulatory system involved in the regulation of the [NiFe] hydrogenase activity. HupR1 is probably phosphorylated by the sensory component and then acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG0784

COG function: function code T; FOG: CheY-like receiver

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 18604; Mature: 18604

Theoretical pI: Translated: 4.04; Mature: 4.04

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKYLILCVDDEPEVLNSVLQDLAPFEDNFIVEGAESVDEAKQVVQEMGQEGIKLALILC
CCCEEEEEECCCHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHCCCCCEEHHHHH
DHIMPDKTGIDFLIELNQHDSTKPTRKLLLTGQAGLEDTVTAINNAALDFYISKPWQGDQ
HHCCCCCCCCEEEEEECCCCCCCHHHHEEECCCCCCHHHHHHHHHHHEEEEECCCCCCHH
LRETITQQLTDYVIANDKQLLNWTSILDTERILTSMSDKRTSFSE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNKYLILCVDDEPEVLNSVLQDLAPFEDNFIVEGAESVDEAKQVVQEMGQEGIKLALILC
CCCEEEEEECCCHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHCCCCCEEHHHHH
DHIMPDKTGIDFLIELNQHDSTKPTRKLLLTGQAGLEDTVTAINNAALDFYISKPWQGDQ
HHCCCCCCCCEEEEEECCCCCCCHHHHEEECCCCCCHHHHHHHHHHHEEEEECCCCCCHH
LRETITQQLTDYVIANDKQLLNWTSILDTERILTSMSDKRTSFSE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1885559; 8497190; 2007559 [H]