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The map label for this gene is folE [H]

Identifier: 218709738

GI number: 218709738

Start: 1871946

End: 1872584

Strand: Direct

Name: folE [H]

Synonym: VS_1750

Alternate gene names: 218709738

Gene position: 1871946-1872584 (Clockwise)

Preceding gene: 218709737

Following gene: 218709739

Centisome position: 56.74

GC content: 42.25

Gene sequence:

>639_bases
ATGCTGAACACAGAAGCCGAAAAAGTAAGAGAAGCTTTGCTCGCAAGAGGACTTGAAACTCCAATGACACCGAGCGAAAT
GAATCCCAACCAGAAGTACAACCGCATCAAGGGACTCTTAACAGAAGTTGTCAGTACGCTTGGACTGGATTTAAACGATG
ACAGCCTTGCTGAAACACCTCATCGCATTGCAAAGATGTACGTCCATGAGATTTTTTCAGGGCTTGATTACGATAATTTC
CCAAAAATCAGTGTTATAGAAAATAAAATGTCGGTTGATGAAATGGTTAAGGTATCGGATATCGACTTAACATCTACGTG
CGAACATCACTTCATTACTATTGATGGTTTAGCGGAAGTGGCTTATATACCTGAAAACAAAATTCTTGGGCTGTCTAAAA
TCAACCGTATCGTTCGATTCTTTGCTCAGCGTCCTCAGGTACAAGAACGCCTTACACAACAAATCTTGGTTGCGATACAG
ACTCTCGTTGAAACAGAAAACGTGGCAGTGACGATTAAAGCGTCGCACTATTGCGTTAAATCCAGAGGCGTCATGGATGC
AAACTCTGAAACCTCAACGACTGCACTCGGTGGCATTTTCAAAACTAACCCTCAAACCAGAGCTGAGTTTTTACGATGA

Upstream 100 bases:

>100_bases
GTGAGAATCGACAAGCCTCATGCGCTACGTTTTGCTGACTCTGTTTCGCTCACTCTGAGCTATGAAGCAGACAACGACAA
TATTTCATAAGGAGTGCGTC

Downstream 100 bases:

>100_bases
GTGAAACAATCCTGATAACCGGCGTGGGAAAGCGACTCGGATTCGCACTGGCGCAGCAACTTTTAGCGGATGGATACAAT
GTGGTTGGCACTTACCGCAA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MLNTEAEKVREALLARGLETPMTPSEMNPNQKYNRIKGLLTEVVSTLGLDLNDDSLAETPHRIAKMYVHEIFSGLDYDNF
PKISVIENKMSVDEMVKVSDIDLTSTCEHHFITIDGLAEVAYIPENKILGLSKINRIVRFFAQRPQVQERLTQQILVAIQ
TLVETENVAVTIKASHYCVKSRGVMDANSETSTTALGGIFKTNPQTRAEFLR

Sequences:

>Translated_212_residues
MLNTEAEKVREALLARGLETPMTPSEMNPNQKYNRIKGLLTEVVSTLGLDLNDDSLAETPHRIAKMYVHEIFSGLDYDNF
PKISVIENKMSVDEMVKVSDIDLTSTCEHHFITIDGLAEVAYIPENKILGLSKINRIVRFFAQRPQVQERLTQQILVAIQ
TLVETENVAVTIKASHYCVKSRGVMDANSETSTTALGGIFKTNPQTRAEFLR
>Mature_212_residues
MLNTEAEKVREALLARGLETPMTPSEMNPNQKYNRIKGLLTEVVSTLGLDLNDDSLAETPHRIAKMYVHEIFSGLDYDNF
PKISVIENKMSVDEMVKVSDIDLTSTCEHHFITIDGLAEVAYIPENKILGLSKINRIVRFFAQRPQVQERLTQQILVAIQ
TLVETENVAVTIKASHYCVKSRGVMDANSETSTTALGGIFKTNPQTRAEFLR

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=178, Percent_Identity=39.3258426966292, Blast_Score=125, Evalue=2e-29,
Organism=Homo sapiens, GI4503949, Length=178, Percent_Identity=39.3258426966292, Blast_Score=125, Evalue=2e-29,
Organism=Homo sapiens, GI66932970, Length=151, Percent_Identity=35.7615894039735, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI66932972, Length=142, Percent_Identity=37.3239436619718, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1788476, Length=213, Percent_Identity=68.5446009389671, Blast_Score=290, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI17560486, Length=171, Percent_Identity=42.6900584795322, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6321706, Length=182, Percent_Identity=39.5604395604396, Blast_Score=133, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24656782, Length=179, Percent_Identity=39.1061452513966, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24656791, Length=179, Percent_Identity=39.1061452513966, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24656796, Length=179, Percent_Identity=39.1061452513966, Blast_Score=121, Evalue=3e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 23797; Mature: 23797

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNTEAEKVREALLARGLETPMTPSEMNPNQKYNRIKGLLTEVVSTLGLDLNDDSLAETP
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
HRIAKMYVHEIFSGLDYDNFPKISVIENKMSVDEMVKVSDIDLTSTCEHHFITIDGLAEV
HHHHHHHHHHHHHCCCCCCCCCEEEEHHHHCHHHHHHHCCCCCCCCCCCCEEEECCHHHH
AYIPENKILGLSKINRIVRFFAQRPQVQERLTQQILVAIQTLVETENVAVTIKASHYCVK
EECCCCCEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEC
SRGVMDANSETSTTALGGIFKTNPQTRAEFLR
CCCCCCCCCCCCHHHHCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MLNTEAEKVREALLARGLETPMTPSEMNPNQKYNRIKGLLTEVVSTLGLDLNDDSLAETP
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
HRIAKMYVHEIFSGLDYDNFPKISVIENKMSVDEMVKVSDIDLTSTCEHHFITIDGLAEV
HHHHHHHHHHHHHCCCCCCCCCEEEEHHHHCHHHHHHHCCCCCCCCCCCCEEEECCHHHH
AYIPENKILGLSKINRIVRFFAQRPQVQERLTQQILVAIQTLVETENVAVTIKASHYCVK
EECCCCCEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEC
SRGVMDANSETSTTALGGIFKTNPQTRAEFLR
CCCCCCCCCCCCHHHHCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]