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The map label for this gene is rlmF [H]

Identifier: 218709668

GI number: 218709668

Start: 1805483

End: 1806835

Strand: Reverse

Name: rlmF [H]

Synonym: VS_1679

Alternate gene names: 218709668

Gene position: 1806835-1805483 (Counterclockwise)

Preceding gene: 218709669

Following gene: 218709665

Centisome position: 54.76

GC content: 44.05

Gene sequence:

>1353_bases
ATGAGCCAATCACAAAATAGAACGACGCTAAGCCTGTCGAAAAACAGTACCTCCGCAGCAAAAACAAAAGATAACCAAGG
TAACGAAGGTAACAGTACGCAAGGTAATCGCAAGCAAGGTGATGTTAAGCCAAGTGCAGGTAAACCAAGCAGCGACAAGC
GTATTTCTTCAAAGAACACATCGGATAAAAACCGAAAGAGTACAGTTTCAAAAGGCGCTAGTAACAACAAAGCAAAGCGA
CCTTTTGCAAAAGATAGCAAAGGTAAGCCGGGCGCTAATAAAGGGCCATCGAATAAGGCGTCTTTCAATAAAACTGTATT
TAACAAGAATGCAGCACAGAAAAGACGTTCGGCAGAGAAGCCTCAAGGTGGATTACACCCAAGAAACCAACACACAGGTC
GCTACGACTTTGAGTTATTGGTTGCAGCGTTACCTGAGCTAAAAGAACATCTGATCAAGAACCCGGTGGGCGAAGACACC
GTTAATTTCTCTGATCCATTGGCTGTTAAGCTACTTAACAAGGCTTTACTGGCGCATCACTATGGCGTTAAGCATTGGGA
TATTCCTGCTGGTTACTTGTGCCCACCAATTCCTGGCCGTGCAGATTATATCCACAGAGTGGCAGACATCTTAAATAGCG
ATGGACAAGGTGAACCTTATAATCATGCCTCTGTGAAAGCATTAGATATCGGTGTTGGTGCAAACTGTATTTACCCAATC
ATTGGCGCGACAGAGTATAAGTGGCGCTGTACGGGTACGGACGTCGATTCAGTATCCATCAAAACAGCGAACTTCATTGC
TGAAAGTAACCCTAATCTAAAAGGTAAGATCCGAGCTCGCCTGCAAGCGGACTCTGAATCTATCTTTAAGGGCGTGATTA
AGGATAATGAACGTTACGATGTCACTATTTGTAACCCTCCATTCCATAGTTCTCTAGAAGAGGCGGAAAAGGGATCACAA
CGTAAATTGGATAACTTAGCGGCAAACCGAGCTAAGAAAGCGGGCAAGTCATTCAAGCCCGAGATGAATAAGAAGCCAGC
CAAATCGATTAAGCAAGAGAAACCAACCTTAAACTTTGGTGGCCAAAAATCTGAACTTTGGTGTCCGGGTGGCGAAGCTA
CTTTCATTATGAAAATGGCGAGAGAGAGCCAACTTTTTGCTACTCAAGTATTGTGGTTCACGACATTGATTTCTAAGAAA
GATAACGTCGATATGGTTCGTTCAGAGCTGGGTAAGCTAAGAGCTAAACAAGTAAAAGTGGTTGAGATGTCGCAAGGCCA
GAAGGTGAGTCGCTTTATCGCATGGACCTTTATGGACGACGAGCAAAGACAAGAGTGGATTGCGCTAAAATAG

Upstream 100 bases:

>100_bases
GCCTTTTACCAACTATGCTGTCACATGGAACCGGAATATCATCAACTTGAGTTAGATGCCCGTTTATTCATGATGTATTT
AGATTTAAAGAAGTAGCCCA

Downstream 100 bases:

>100_bases
CGGCAGCACTGTGTTAAGACATTAAGTTGAAATTACGTACGGGCTTGTTGTTAACGCAACAAGCCCGTTTTTTGTGTTTT
TCATATGCGCTAAGCAGAAA

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MSQSQNRTTLSLSKNSTSAAKTKDNQGNEGNSTQGNRKQGDVKPSAGKPSSDKRISSKNTSDKNRKSTVSKGASNNKAKR
PFAKDSKGKPGANKGPSNKASFNKTVFNKNAAQKRRSAEKPQGGLHPRNQHTGRYDFELLVAALPELKEHLIKNPVGEDT
VNFSDPLAVKLLNKALLAHHYGVKHWDIPAGYLCPPIPGRADYIHRVADILNSDGQGEPYNHASVKALDIGVGANCIYPI
IGATEYKWRCTGTDVDSVSIKTANFIAESNPNLKGKIRARLQADSESIFKGVIKDNERYDVTICNPPFHSSLEEAEKGSQ
RKLDNLAANRAKKAGKSFKPEMNKKPAKSIKQEKPTLNFGGQKSELWCPGGEATFIMKMARESQLFATQVLWFTTLISKK
DNVDMVRSELGKLRAKQVKVVEMSQGQKVSRFIAWTFMDDEQRQEWIALK

Sequences:

>Translated_450_residues
MSQSQNRTTLSLSKNSTSAAKTKDNQGNEGNSTQGNRKQGDVKPSAGKPSSDKRISSKNTSDKNRKSTVSKGASNNKAKR
PFAKDSKGKPGANKGPSNKASFNKTVFNKNAAQKRRSAEKPQGGLHPRNQHTGRYDFELLVAALPELKEHLIKNPVGEDT
VNFSDPLAVKLLNKALLAHHYGVKHWDIPAGYLCPPIPGRADYIHRVADILNSDGQGEPYNHASVKALDIGVGANCIYPI
IGATEYKWRCTGTDVDSVSIKTANFIAESNPNLKGKIRARLQADSESIFKGVIKDNERYDVTICNPPFHSSLEEAEKGSQ
RKLDNLAANRAKKAGKSFKPEMNKKPAKSIKQEKPTLNFGGQKSELWCPGGEATFIMKMARESQLFATQVLWFTTLISKK
DNVDMVRSELGKLRAKQVKVVEMSQGQKVSRFIAWTFMDDEQRQEWIALK
>Mature_449_residues
SQSQNRTTLSLSKNSTSAAKTKDNQGNEGNSTQGNRKQGDVKPSAGKPSSDKRISSKNTSDKNRKSTVSKGASNNKAKRP
FAKDSKGKPGANKGPSNKASFNKTVFNKNAAQKRRSAEKPQGGLHPRNQHTGRYDFELLVAALPELKEHLIKNPVGEDTV
NFSDPLAVKLLNKALLAHHYGVKHWDIPAGYLCPPIPGRADYIHRVADILNSDGQGEPYNHASVKALDIGVGANCIYPII
GATEYKWRCTGTDVDSVSIKTANFIAESNPNLKGKIRARLQADSESIFKGVIKDNERYDVTICNPPFHSSLEEAEKGSQR
KLDNLAANRAKKAGKSFKPEMNKKPAKSIKQEKPTLNFGGQKSELWCPGGEATFIMKMARESQLFATQVLWFTTLISKKD
NVDMVRSELGKLRAKQVKVVEMSQGQKVSRFIAWTFMDDEQRQEWIALK

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA [H]

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family [H]

Homologues:

Organism=Homo sapiens, GI122114654, Length=319, Percent_Identity=28.8401253918495, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI87081786, Length=331, Percent_Identity=51.6616314199396, Blast_Score=342, Evalue=3e-95,
Organism=Caenorhabditis elegans, GI32564931, Length=318, Percent_Identity=23.8993710691824, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32564933, Length=318, Percent_Identity=21.6981132075472, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI281363415, Length=324, Percent_Identity=26.5432098765432, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922302, Length=324, Percent_Identity=26.5432098765432, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016909
- InterPro:   IPR010286 [H]

Pfam domain/function: PF05971 Methyltransf_10 [H]

EC number: =2.1.1.181 [H]

Molecular weight: Translated: 49695; Mature: 49564

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSQNRTTLSLSKNSTSAAKTKDNQGNEGNSTQGNRKQGDVKPSAGKPSSDKRISSKNT
CCCCCCCEEEEEECCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SDKNRKSTVSKGASNNKAKRPFAKDSKGKPGANKGPSNKASFNKTVFNKNAAQKRRSAEK
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHCCC
PQGGLHPRNQHTGRYDFELLVAALPELKEHLIKNPVGEDTVNFSDPLAVKLLNKALLAHH
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
YGVKHWDIPAGYLCPPIPGRADYIHRVADILNSDGQGEPYNHASVKALDIGVGANCIYPI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHC
IGATEYKWRCTGTDVDSVSIKTANFIAESNPNLKGKIRARLQADSESIFKGVIKDNERYD
CCCCCEEEEECCCCCCCEEEEHHHHHCCCCCCCCEEEEEEEECCHHHHHHHHHCCCCEEE
VTICNPPFHSSLEEAEKGSQRKLDNLAANRAKKAGKSFKPEMNKKPAKSIKQEKPTLNFG
EEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCC
GQKSELWCPGGEATFIMKMARESQLFATQVLWFTTLISKKDNVDMVRSELGKLRAKQVKV
CCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEE
VEMSQGQKVSRFIAWTFMDDEQRQEWIALK
EECCCCCCEEEEEEEEEECCHHHHHHCCCC
>Mature Secondary Structure 
SQSQNRTTLSLSKNSTSAAKTKDNQGNEGNSTQGNRKQGDVKPSAGKPSSDKRISSKNT
CCCCCCEEEEEECCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SDKNRKSTVSKGASNNKAKRPFAKDSKGKPGANKGPSNKASFNKTVFNKNAAQKRRSAEK
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHCCC
PQGGLHPRNQHTGRYDFELLVAALPELKEHLIKNPVGEDTVNFSDPLAVKLLNKALLAHH
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
YGVKHWDIPAGYLCPPIPGRADYIHRVADILNSDGQGEPYNHASVKALDIGVGANCIYPI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHC
IGATEYKWRCTGTDVDSVSIKTANFIAESNPNLKGKIRARLQADSESIFKGVIKDNERYD
CCCCCEEEEECCCCCCCEEEEHHHHHCCCCCCCCEEEEEEEECCHHHHHHHHHCCCCEEE
VTICNPPFHSSLEEAEKGSQRKLDNLAANRAKKAGKSFKPEMNKKPAKSIKQEKPTLNFG
EEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCC
GQKSELWCPGGEATFIMKMARESQLFATQVLWFTTLISKKDNVDMVRSELGKLRAKQVKV
CCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEE
VEMSQGQKVSRFIAWTFMDDEQRQEWIALK
EECCCCCCEEEEEEEEEECCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA