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The map label for this gene is ycdT [H]

Identifier: 218709662

GI number: 218709662

Start: 1798904

End: 1800862

Strand: Reverse

Name: ycdT [H]

Synonym: VS_1673

Alternate gene names: 218709662

Gene position: 1800862-1798904 (Counterclockwise)

Preceding gene: 218709664

Following gene: 218709660

Centisome position: 54.58

GC content: 40.74

Gene sequence:

>1959_bases
ATGAATAACAACAGAGTGTTAGGCTCATTTTTTCTCGTCGTACTGATCAGTGTTGTACTAGGATTGTATTTGCTCTATCC
AACTTATGACGACGATTTACAGACTATTCGACCAGAAACCCCAGGCGCGTACAGTCCCGAAAAAGAGTTATCAGCGACTC
AATATGGCGCTGAACTCATTGAAATCCTTAATGTGTCTCGCGGTGATCCAAAGCTTGCCTCCCAAAAGCTTGAAACCTTA
AAAGCAACGAGCCCTACGCAACATAAAGCCATCTATCGTGGTTATGAACTAATGATTTTATCGAATCTCGCACAGCATGA
TCTCGATAAACAGGCTGTGTCTGAATACACACGACAGATCAAGGCATTAGCACAGCGTGAAGATATGGGGTGGTTAAAGG
CGCAATCTTTAGTTGAACTAGCGATTGAGCAGATGAGTCAAGGTGAATTAGTAGAGGCTGAATCTCAAGTTAAAGCAGCA
ATAAGTATCGCTGAATCAATCCAGTATGAAGAACTGTTGATAAAAGCCTACAACACTGTGGGGTCGATCAAAAACATCCA
AAATGATTTTTCCGAAGCTCAGTATTTTTTTCATAAGGGCTTAAATCTTGGCAAGAAATACCCACGGCATATCTATAACA
GTAAGTTGATGTCGAATATGGCATTGCTTTATATCTATCTGGAAGATTGGCCGAAAGCACTCAAACTTATCGAACAGGCC
AAAAAACTCTATATCAAAAGTGGACTACGCGATGGTTCAACCATCGGTGTGTTATATGTTAATGAATCATTTGCTTATCT
ACGCAGTGGCGACTTAGTAAAAGGTCGAGAGGCTTATAAAAGTGCCACCAAATTTAATGATGATAGCGTAAGTGAAAGGT
ACAAAATCCTTCTTCTAAAAGCTCAGAGTGAAGTACTGCTAGCAGAAAAGAACTTTGAGTTAGCAAGGAAAGTAACAAAC
CAATGTATTGGTTACCCGGGTGTTGCAAAGTACACACTCCAACTGGGTCAGTGTTACTTAAACAGAGCTTTAGCAAATAT
CGGTTTGAACCAAGATAACTTTGTTTTCTTTGATCTAAAACGTTCGTCTACGATGTTTGATATTGTTGGCAGCCGTAGTT
GGAAGGTGCTTGGTTTCAAAGCGCTTGCAGAATACTATGAATCTAAAGGCAACAGTGAAATTGGACTTCGGTATTTCAAG
CTTTATTACAAGGGCAACAAAGCGCTGTTATTTGAAAAGCGTCAGAGTGATATCTTTTTATTAGAACAAGACTTTGCTAC
GGCTTCATTAGCGAAAGAGAATGAACTGCTTAATGCAGAAAAAGAGCTCGCAGAGATGACGCTAGATAAACAGAGCTTAA
AAGACCGCATTGTTGTCGTACTCGGTGTTATGGTCATCATAAGCTTTGCTGTACTTCTGGTAAGGTTATGCACAATGCAT
AAGACCAACAAAGAGTTGGTTAAGCAATCGACGAGATGTGAGTTGACAGGTTTATTCAATAGACGCTACCTGGAACAATT
GTTAACTCAGCCAATGCCATACCGTTTGTCGCCGTTCGGGTATACGCTTGCTATTTTGGACTTAGATCAATTTAAACGAG
TCAATGATACATACGGACATGATATTGGGGACCAAGTTTTGGTAGAGGTTGCTAAGCGTATAGAGCGAGCGCTATCAACA
AACGATGTGTTGGCTCGATGGGGTGGGGAAGAATTCGTTTTACTGCTTGCTGGCACTCAAGACCCAGACGAACAGTTGGA
AATGATAAGGCAAACAATCGCTGATCAAACGATAGAAACGTATTCCGGTCCATTAGCGTTAACTACATCAATTGGTGCGA
CCATTCATTTAACGTATCAAGAATTAAATAGCAGTACCTACAAACAGCATTTGAAATCAGCAGATGACGCTTTATATCAA
GCAAAACAATCAGGGCGCAACCAGGTGGTCATTGCTTAG

Upstream 100 bases:

>100_bases
ATGTGCAGGATTTAAATCTCCATATGTGTCGGGTACAATGTGAAACCGCTTAAGGTAACCGTCGATTAAATTGGTATGCA
ATCAGTAATGGCACTTATAA

Downstream 100 bases:

>100_bases
CGAAAAATACAGCTTAGTGCGAAAATACAGCTTAAATGAACCTGTAATTTAAATACTTGGCAGCCTAATCAGAATCATTC
CCCTTAGGCAGAAGTGTTTA

Product: GGDEF family protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 652; Mature: 652

Protein sequence:

>652_residues
MNNNRVLGSFFLVVLISVVLGLYLLYPTYDDDLQTIRPETPGAYSPEKELSATQYGAELIEILNVSRGDPKLASQKLETL
KATSPTQHKAIYRGYELMILSNLAQHDLDKQAVSEYTRQIKALAQREDMGWLKAQSLVELAIEQMSQGELVEAESQVKAA
ISIAESIQYEELLIKAYNTVGSIKNIQNDFSEAQYFFHKGLNLGKKYPRHIYNSKLMSNMALLYIYLEDWPKALKLIEQA
KKLYIKSGLRDGSTIGVLYVNESFAYLRSGDLVKGREAYKSATKFNDDSVSERYKILLLKAQSEVLLAEKNFELARKVTN
QCIGYPGVAKYTLQLGQCYLNRALANIGLNQDNFVFFDLKRSSTMFDIVGSRSWKVLGFKALAEYYESKGNSEIGLRYFK
LYYKGNKALLFEKRQSDIFLLEQDFATASLAKENELLNAEKELAEMTLDKQSLKDRIVVVLGVMVIISFAVLLVRLCTMH
KTNKELVKQSTRCELTGLFNRRYLEQLLTQPMPYRLSPFGYTLAILDLDQFKRVNDTYGHDIGDQVLVEVAKRIERALST
NDVLARWGGEEFVLLLAGTQDPDEQLEMIRQTIADQTIETYSGPLALTTSIGATIHLTYQELNSSTYKQHLKSADDALYQ
AKQSGRNQVVIA

Sequences:

>Translated_652_residues
MNNNRVLGSFFLVVLISVVLGLYLLYPTYDDDLQTIRPETPGAYSPEKELSATQYGAELIEILNVSRGDPKLASQKLETL
KATSPTQHKAIYRGYELMILSNLAQHDLDKQAVSEYTRQIKALAQREDMGWLKAQSLVELAIEQMSQGELVEAESQVKAA
ISIAESIQYEELLIKAYNTVGSIKNIQNDFSEAQYFFHKGLNLGKKYPRHIYNSKLMSNMALLYIYLEDWPKALKLIEQA
KKLYIKSGLRDGSTIGVLYVNESFAYLRSGDLVKGREAYKSATKFNDDSVSERYKILLLKAQSEVLLAEKNFELARKVTN
QCIGYPGVAKYTLQLGQCYLNRALANIGLNQDNFVFFDLKRSSTMFDIVGSRSWKVLGFKALAEYYESKGNSEIGLRYFK
LYYKGNKALLFEKRQSDIFLLEQDFATASLAKENELLNAEKELAEMTLDKQSLKDRIVVVLGVMVIISFAVLLVRLCTMH
KTNKELVKQSTRCELTGLFNRRYLEQLLTQPMPYRLSPFGYTLAILDLDQFKRVNDTYGHDIGDQVLVEVAKRIERALST
NDVLARWGGEEFVLLLAGTQDPDEQLEMIRQTIADQTIETYSGPLALTTSIGATIHLTYQELNSSTYKQHLKSADDALYQ
AKQSGRNQVVIA
>Mature_652_residues
MNNNRVLGSFFLVVLISVVLGLYLLYPTYDDDLQTIRPETPGAYSPEKELSATQYGAELIEILNVSRGDPKLASQKLETL
KATSPTQHKAIYRGYELMILSNLAQHDLDKQAVSEYTRQIKALAQREDMGWLKAQSLVELAIEQMSQGELVEAESQVKAA
ISIAESIQYEELLIKAYNTVGSIKNIQNDFSEAQYFFHKGLNLGKKYPRHIYNSKLMSNMALLYIYLEDWPKALKLIEQA
KKLYIKSGLRDGSTIGVLYVNESFAYLRSGDLVKGREAYKSATKFNDDSVSERYKILLLKAQSEVLLAEKNFELARKVTN
QCIGYPGVAKYTLQLGQCYLNRALANIGLNQDNFVFFDLKRSSTMFDIVGSRSWKVLGFKALAEYYESKGNSEIGLRYFK
LYYKGNKALLFEKRQSDIFLLEQDFATASLAKENELLNAEKELAEMTLDKQSLKDRIVVVLGVMVIISFAVLLVRLCTMH
KTNKELVKQSTRCELTGLFNRRYLEQLLTQPMPYRLSPFGYTLAILDLDQFKRVNDTYGHDIGDQVLVEVAKRIERALST
NDVLARWGGEEFVLLLAGTQDPDEQLEMIRQTIADQTIETYSGPLALTTSIGATIHLTYQELNSSTYKQHLKSADDALYQ
AKQSGRNQVVIA

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=192, Percent_Identity=35.9375, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI1786584, Length=177, Percent_Identity=36.7231638418079, Blast_Score=109, Evalue=6e-25,
Organism=Escherichia coli, GI87081881, Length=187, Percent_Identity=39.0374331550802, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI145693134, Length=176, Percent_Identity=32.3863636363636, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI87082007, Length=192, Percent_Identity=31.25, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788085, Length=165, Percent_Identity=33.9393939393939, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=31.6455696202532, Blast_Score=81, Evalue=3e-16,
Organism=Escherichia coli, GI1787802, Length=130, Percent_Identity=35.3846153846154, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1787541, Length=158, Percent_Identity=34.1772151898734, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI87081977, Length=176, Percent_Identity=30.1136363636364, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1788381, Length=138, Percent_Identity=35.5072463768116, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787056, Length=166, Percent_Identity=30.1204819277108, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788956, Length=171, Percent_Identity=28.6549707602339, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87081974, Length=192, Percent_Identity=28.6458333333333, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 73902; Mature: 73902

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS50293 TPR_REGION ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNNRVLGSFFLVVLISVVLGLYLLYPTYDDDLQTIRPETPGAYSPEKELSATQYGAELI
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHH
EILNVSRGDPKLASQKLETLKATSPTQHKAIYRGYELMILSNLAQHDLDKQAVSEYTRQI
HHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHH
KALAQREDMGWLKAQSLVELAIEQMSQGELVEAESQVKAAISIAESIQYEELLIKAYNTV
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSIKNIQNDFSEAQYFFHKGLNLGKKYPRHIYNSKLMSNMALLYIYLEDWPKALKLIEQA
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHH
KKLYIKSGLRDGSTIGVLYVNESFAYLRSGDLVKGREAYKSATKFNDDSVSERYKILLLK
HHHHHHCCCCCCCEEEEEEEECCCHHHCCCCCCCCHHHHHHHHCCCCCCHHCCEEEEEEE
AQSEVLLAEKNFELARKVTNQCIGYPGVAKYTLQLGQCYLNRALANIGLNQDNFVFFDLK
CCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
RSSTMFDIVGSRSWKVLGFKALAEYYESKGNSEIGLRYFKLYYKGNKALLFEKRQSDIFL
CCCCEEEECCCCCEEEHHHHHHHHHHHCCCCCCCCEEEEEEEEECCEEEEEEECCCCEEE
LEQDFATASLAKENELLNAEKELAEMTLDKQSLKDRIVVVLGVMVIISFAVLLVRLCTMH
EECCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KTNKELVKQSTRCELTGLFNRRYLEQLLTQPMPYRLSPFGYTLAILDLDQFKRVNDTYGH
CCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHHHHHHCCCCCC
DIGDQVLVEVAKRIERALSTNDVLARWGGEEFVLLLAGTQDPDEQLEMIRQTIADQTIET
CCHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
YSGPLALTTSIGATIHLTYQELNSSTYKQHLKSADDALYQAKQSGRNQVVIA
CCCCEEEEEECCCEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MNNNRVLGSFFLVVLISVVLGLYLLYPTYDDDLQTIRPETPGAYSPEKELSATQYGAELI
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHH
EILNVSRGDPKLASQKLETLKATSPTQHKAIYRGYELMILSNLAQHDLDKQAVSEYTRQI
HHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHH
KALAQREDMGWLKAQSLVELAIEQMSQGELVEAESQVKAAISIAESIQYEELLIKAYNTV
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSIKNIQNDFSEAQYFFHKGLNLGKKYPRHIYNSKLMSNMALLYIYLEDWPKALKLIEQA
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHH
KKLYIKSGLRDGSTIGVLYVNESFAYLRSGDLVKGREAYKSATKFNDDSVSERYKILLLK
HHHHHHCCCCCCCEEEEEEEECCCHHHCCCCCCCCHHHHHHHHCCCCCCHHCCEEEEEEE
AQSEVLLAEKNFELARKVTNQCIGYPGVAKYTLQLGQCYLNRALANIGLNQDNFVFFDLK
CCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
RSSTMFDIVGSRSWKVLGFKALAEYYESKGNSEIGLRYFKLYYKGNKALLFEKRQSDIFL
CCCCEEEECCCCCEEEHHHHHHHHHHHCCCCCCCCEEEEEEEEECCEEEEEEECCCCEEE
LEQDFATASLAKENELLNAEKELAEMTLDKQSLKDRIVVVLGVMVIISFAVLLVRLCTMH
EECCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KTNKELVKQSTRCELTGLFNRRYLEQLLTQPMPYRLSPFGYTLAILDLDQFKRVNDTYGH
CCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHHHHHHCCCCCC
DIGDQVLVEVAKRIERALSTNDVLARWGGEEFVLLLAGTQDPDEQLEMIRQTIADQTIET
CCHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
YSGPLALTTSIGATIHLTYQELNSSTYKQHLKSADDALYQAKQSGRNQVVIA
CCCCEEEEEECCCEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]