Click here to switch to the map view.

The map label for this gene is intA [H]

Identifier: 218709659

GI number: 218709659

Start: 1795776

End: 1797017

Strand: Reverse

Name: intA [H]

Synonym: VS_1669

Alternate gene names: 218709659

Gene position: 1797017-1795776 (Counterclockwise)

Preceding gene: 218709660

Following gene: 218709658

Centisome position: 54.47

GC content: 42.91

Gene sequence:

>1242_bases
ATGGCGAAAAAAGTACAGCCTTTAACGGCAACTCAAATCAAACAAGCGAAGCCTAAAGAGAAAGAATATAATTTAGGTGA
CGGTGATGGATTATCATTGAAGATTAAGCCTATAGGAACAAAACTCTGGCGTTTCTCTTATTATCATCCCATTTCACGAA
AGCGCTTGAACCTAGCCTTAGGTAAGTACCCTGACGTCTCACTAGCCAAAGCTCGAACCAAAGCTATTGAAGCTAGAGAA
CTACTTGCTGATGGTATTGACCCTAAAGAGCACCGTGATGCGACACTCAGAGAAAAACAGTTAGAATTGGCGAATACGTT
ACAGTCGGTATTTGATGACTGGATTGAAGTGAAGAGAACAAGCGTCAAAGAGCAAACGGCTAAGAAGCTAAAGCAACGAA
TTGAGAAGTACCTCTTACCTGATCTTGGTAAGTTCCCCCTTAGTGACATTACGGCACCACAAGCGATAAAAACAATTAAA
CCCATTGCGAATCAAGGTAAATTAGAAACAGTAGGGCGTTTGTGTCGTAACCTCAATGAGATAATGACCTTTGCAGTAAA
CACGGGTGTGATTGAACACAACAAGCTTGCTGGCATTGGTAAAGCATTTGAGACTGCTAAGGTAACCAACCAAGCTTCGT
TAGCCCCAGATGAGCTTCCAGAGCTTCTCAAAACCATCAACTACGCATCGATTAAACCCATCACTCGCTGCTTGCTAGAA
TGGCAACTTCACACAATGACAAGGCCAGCTGAAACGGCTGGGGCACGTTGGGATGAAATTGACAGAGAAAAGATGCTTTG
GGTAATTCCTGCTGAACGAATGAAAAAGGGAAGAGTGCATACTGTTCCTTTAACAAAACAAACTCTAGATCTATTGGAAA
CCATTGATTTCATCACGGGTGATAGTGAATACCTTTTCCCAGCAGACAAAAGTAACAAAAATCACATCAACAAAGAGACA
GCAAATAAAGCATTGGTACGAATGGGCATGAAGGGTAGGCAAACTTCTCACGGTTTGCGCGCATTAGCCAGTACCACCTT
AAATGAGCAAAACTTCGATCCAGATATTATAGAAGCCGCACTTTCTCATGTGGATAGAGACAAGGTAAGAAGCGCATACA
ACCGCACTGATTACCTTGAACGCCGTAAAGTTCTAATGGCGTGGTGGAGTGATCATATCGAAGAAGCTGCTTCGGGGAGC
TTGTCGATGAGTGGTAAAAAGAGCCTGAAGGTTGTGGGTTAA

Upstream 100 bases:

>100_bases
AAGAGATTCCATGATGTGTTGTAAAATCCACCGTTTAAAGTAATCTCTGAAGTAGTCTTTTATGATCTAAGCAATTTCGA
GGTTACTTCAGGGGGTTACG

Downstream 100 bases:

>100_bases
TAGGTACATGTAATTTGATTTCAGTAAACTAATGTTACACTGTAACTCGCGTTCTTCAATGAATTTAGAAGTCGGGCAGG
GATTGGCATAGGGTACGGTT

Product: phage integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MAKKVQPLTATQIKQAKPKEKEYNLGDGDGLSLKIKPIGTKLWRFSYYHPISRKRLNLALGKYPDVSLAKARTKAIEARE
LLADGIDPKEHRDATLREKQLELANTLQSVFDDWIEVKRTSVKEQTAKKLKQRIEKYLLPDLGKFPLSDITAPQAIKTIK
PIANQGKLETVGRLCRNLNEIMTFAVNTGVIEHNKLAGIGKAFETAKVTNQASLAPDELPELLKTINYASIKPITRCLLE
WQLHTMTRPAETAGARWDEIDREKMLWVIPAERMKKGRVHTVPLTKQTLDLLETIDFITGDSEYLFPADKSNKNHINKET
ANKALVRMGMKGRQTSHGLRALASTTLNEQNFDPDIIEAALSHVDRDKVRSAYNRTDYLERRKVLMAWWSDHIEEAASGS
LSMSGKKSLKVVG

Sequences:

>Translated_413_residues
MAKKVQPLTATQIKQAKPKEKEYNLGDGDGLSLKIKPIGTKLWRFSYYHPISRKRLNLALGKYPDVSLAKARTKAIEARE
LLADGIDPKEHRDATLREKQLELANTLQSVFDDWIEVKRTSVKEQTAKKLKQRIEKYLLPDLGKFPLSDITAPQAIKTIK
PIANQGKLETVGRLCRNLNEIMTFAVNTGVIEHNKLAGIGKAFETAKVTNQASLAPDELPELLKTINYASIKPITRCLLE
WQLHTMTRPAETAGARWDEIDREKMLWVIPAERMKKGRVHTVPLTKQTLDLLETIDFITGDSEYLFPADKSNKNHINKET
ANKALVRMGMKGRQTSHGLRALASTTLNEQNFDPDIIEAALSHVDRDKVRSAYNRTDYLERRKVLMAWWSDHIEEAASGS
LSMSGKKSLKVVG
>Mature_412_residues
AKKVQPLTATQIKQAKPKEKEYNLGDGDGLSLKIKPIGTKLWRFSYYHPISRKRLNLALGKYPDVSLAKARTKAIEAREL
LADGIDPKEHRDATLREKQLELANTLQSVFDDWIEVKRTSVKEQTAKKLKQRIEKYLLPDLGKFPLSDITAPQAIKTIKP
IANQGKLETVGRLCRNLNEIMTFAVNTGVIEHNKLAGIGKAFETAKVTNQASLAPDELPELLKTINYASIKPITRCLLEW
QLHTMTRPAETAGARWDEIDREKMLWVIPAERMKKGRVHTVPLTKQTLDLLETIDFITGDSEYLFPADKSNKNHINKETA
NKALVRMGMKGRQTSHGLRALASTTLNEQNFDPDIIEAALSHVDRDKVRSAYNRTDYLERRKVLMAWWSDHIEEAASGSL
SMSGKKSLKVVG

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=413, Percent_Identity=46.9733656174334, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI145693166, Length=400, Percent_Identity=33.25, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1788690, Length=389, Percent_Identity=30.3341902313625, Blast_Score=196, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 46628; Mature: 46497

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKVQPLTATQIKQAKPKEKEYNLGDGDGLSLKIKPIGTKLWRFSYYHPISRKRLNLAL
CCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCHHHHHHHH
GKYPDVSLAKARTKAIEARELLADGIDPKEHRDATLREKQLELANTLQSVFDDWIEVKRT
CCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVKEQTAKKLKQRIEKYLLPDLGKFPLSDITAPQAIKTIKPIANQGKLETVGRLCRNLNE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IMTFAVNTGVIEHNKLAGIGKAFETAKVTNQASLAPDELPELLKTINYASIKPITRCLLE
HHHHHHHCCCEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
WQLHTMTRPAETAGARWDEIDREKMLWVIPAERMKKGRVHTVPLTKQTLDLLETIDFITG
HHHHHCCCCHHHHCCCHHHCCCCCEEEEECHHHHHCCCEEECCCCHHHHHHHHHHHHHCC
DSEYLFPADKSNKNHINKETANKALVRMGMKGRQTSHGLRALASTTLNEQNFDPDIIEAA
CCCEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHH
LSHVDRDKVRSAYNRTDYLERRKVLMAWWSDHIEEAASGSLSMSGKKSLKVVG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECC
>Mature Secondary Structure 
AKKVQPLTATQIKQAKPKEKEYNLGDGDGLSLKIKPIGTKLWRFSYYHPISRKRLNLAL
CCCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCHHHHHHHH
GKYPDVSLAKARTKAIEARELLADGIDPKEHRDATLREKQLELANTLQSVFDDWIEVKRT
CCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVKEQTAKKLKQRIEKYLLPDLGKFPLSDITAPQAIKTIKPIANQGKLETVGRLCRNLNE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IMTFAVNTGVIEHNKLAGIGKAFETAKVTNQASLAPDELPELLKTINYASIKPITRCLLE
HHHHHHHCCCEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
WQLHTMTRPAETAGARWDEIDREKMLWVIPAERMKKGRVHTVPLTKQTLDLLETIDFITG
HHHHHCCCCHHHHCCCHHHCCCCCEEEEECHHHHHCCCEEECCCCHHHHHHHHHHHHHCC
DSEYLFPADKSNKNHINKETANKALVRMGMKGRQTSHGLRALASTTLNEQNFDPDIIEAA
CCCEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHH
LSHVDRDKVRSAYNRTDYLERRKVLMAWWSDHIEEAASGSLSMSGKKSLKVVG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511583; 9278503; 9205837; 7524073 [H]