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The map label for this gene is citR [H]

Identifier: 218709631

GI number: 218709631

Start: 1762534

End: 1763445

Strand: Reverse

Name: citR [H]

Synonym: VS_1641

Alternate gene names: 218709631

Gene position: 1763445-1762534 (Counterclockwise)

Preceding gene: 218709633

Following gene: 218709630

Centisome position: 53.45

GC content: 43.64

Gene sequence:

>912_bases
ATGATGCTCGATATTAATTTGTTGAAGACGTTTGTGACGCTAGCGGAATATAAGCATTTCGGAAAAGCAGCCAACGCACT
GCATATGACACAACCTAATGTGAGTTTGCACCTGAAACAACTAGAACAACAAACACGCATCAAGTTGATAGAGCGAAGTC
CGTTTCAACTGACTCAAGCAGGTGAAAGGTTATTAGAGACAAGCCAAAGAACGTTACTCGAACTGCAGATTTGTCAGGCC
GATCTCAACGCTATTAACGATCTCAAAATTGGTACTTTGACTATTGCTGTCAGCGACATCATTTCCCGGTTCTTGTTGAT
TCGTCCTTTCCAAAAGTTCAAAGCGCAGTATCCGGGTATCGATCTCACGTTGCTGAATACCACGTCATCTCAAGCATCGA
GCTTAGTTAAGAACGCTCAGGCGGATATTGGATTTGTTATCGCCAAAGAACAGCACAACGAGTCGCTCTATTTTACTAAG
CTTCAAGAGCTTTCATGGTGTGCGTTAGGCGATGGCCTAGACATGCAATACTCTGATAGCAAGACAGGAAACGAGGTTAA
AGGTGACGATATAGAGACAGAACTCACCTTGATTCTACTTGGCCACGATACGAGAACCCGTGACTTCATTGATGAAGGTT
TACCAAGCCTTAATCTACCCAATCATAGAGTCATGGAAGTAGGGAGTGTCGATGCTCAAATCGACTGGGCAGAAGCTGGG
TTTGGTGTCGCCATCATTCCTGAGTTTGCGATATCCACCAAGCAGCATTTGAATTCAAAGGTTACGCCATTGACCAATTT
CTCTAGCACCAGCCTTGGCTATATCGTGAGGCAGAACCAAGTCTTATCTAAAGCGACTAAGCAGTTATTAGGCTGGGTGA
ATGATGAGATTACTCTGTTACAAAACAAATAA

Upstream 100 bases:

>100_bases
TGAGAAATCATGTTAGGCCTACAAAGTGGATAAGAGAAATTAATTATCTTTATTATCTTGATAAGGATTTCATATATCAT
GTAAAAAACCTGGGAGGGAT

Downstream 100 bases:

>100_bases
ACGCCATCACTGACTTTGAGTTACCTGAGCCTGCCAAGTGTGGGCTTTTTGTGTTCTGTTAATCATCGTTTTCTAGCATG
ATAATTCGTAGGTGACTTCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Citrate synthase I repressor [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MMLDINLLKTFVTLAEYKHFGKAANALHMTQPNVSLHLKQLEQQTRIKLIERSPFQLTQAGERLLETSQRTLLELQICQA
DLNAINDLKIGTLTIAVSDIISRFLLIRPFQKFKAQYPGIDLTLLNTTSSQASSLVKNAQADIGFVIAKEQHNESLYFTK
LQELSWCALGDGLDMQYSDSKTGNEVKGDDIETELTLILLGHDTRTRDFIDEGLPSLNLPNHRVMEVGSVDAQIDWAEAG
FGVAIIPEFAISTKQHLNSKVTPLTNFSSTSLGYIVRQNQVLSKATKQLLGWVNDEITLLQNK

Sequences:

>Translated_303_residues
MMLDINLLKTFVTLAEYKHFGKAANALHMTQPNVSLHLKQLEQQTRIKLIERSPFQLTQAGERLLETSQRTLLELQICQA
DLNAINDLKIGTLTIAVSDIISRFLLIRPFQKFKAQYPGIDLTLLNTTSSQASSLVKNAQADIGFVIAKEQHNESLYFTK
LQELSWCALGDGLDMQYSDSKTGNEVKGDDIETELTLILLGHDTRTRDFIDEGLPSLNLPNHRVMEVGSVDAQIDWAEAG
FGVAIIPEFAISTKQHLNSKVTPLTNFSSTSLGYIVRQNQVLSKATKQLLGWVNDEITLLQNK
>Mature_303_residues
MMLDINLLKTFVTLAEYKHFGKAANALHMTQPNVSLHLKQLEQQTRIKLIERSPFQLTQAGERLLETSQRTLLELQICQA
DLNAINDLKIGTLTIAVSDIISRFLLIRPFQKFKAQYPGIDLTLLNTTSSQASSLVKNAQADIGFVIAKEQHNESLYFTK
LQELSWCALGDGLDMQYSDSKTGNEVKGDDIETELTLILLGHDTRTRDFIDEGLPSLNLPNHRVMEVGSVDAQIDWAEAG
FGVAIIPEFAISTKQHLNSKVTPLTNFSSTSLGYIVRQNQVLSKATKQLLGWVNDEITLLQNK

Specific function: Negative regulatory protein for the citA gene for citrate synthase I [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=230, Percent_Identity=27.8260869565217, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI145693105, Length=311, Percent_Identity=24.1157556270096, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788748, Length=298, Percent_Identity=24.496644295302, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI2367136, Length=144, Percent_Identity=30.5555555555556, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789204, Length=295, Percent_Identity=23.0508474576271, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790208, Length=252, Percent_Identity=24.6031746031746, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789639, Length=251, Percent_Identity=25.4980079681275, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33869; Mature: 33869

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLDINLLKTFVTLAEYKHFGKAANALHMTQPNVSLHLKQLEQQTRIKLIERSPFQLTQA
CEEEHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
GERLLETSQRTLLELQICQADLNAINDLKIGTLTIAVSDIISRFLLIRPFQKFKAQYPGI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCHHHHHHHCCCC
DLTLLNTTSSQASSLVKNAQADIGFVIAKEQHNESLYFTKLQELSWCALGDGLDMQYSDS
EEEEEECCCHHHHHHHHHCCCCEEEEEEECCCCCCEEEEHHHHCCEEECCCCCCCCCCCC
KTGNEVKGDDIETELTLILLGHDTRTRDFIDEGLPSLNLPNHRVMEVGSVDAQIDWAEAG
CCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHCCCCCCCCCCEEEEECCCCCEEEHHHCC
FGVAIIPEFAISTKQHLNSKVTPLTNFSSTSLGYIVRQNQVLSKATKQLLGWVNDEITLL
CCEEEECHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEE
QNK
ECC
>Mature Secondary Structure
MMLDINLLKTFVTLAEYKHFGKAANALHMTQPNVSLHLKQLEQQTRIKLIERSPFQLTQA
CEEEHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
GERLLETSQRTLLELQICQADLNAINDLKIGTLTIAVSDIISRFLLIRPFQKFKAQYPGI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCHHHHHHHCCCC
DLTLLNTTSSQASSLVKNAQADIGFVIAKEQHNESLYFTKLQELSWCALGDGLDMQYSDS
EEEEEECCCHHHHHHHHHCCCCEEEEEEECCCCCCEEEEHHHHCCEEECCCCCCCCCCCC
KTGNEVKGDDIETELTLILLGHDTRTRDFIDEGLPSLNLPNHRVMEVGSVDAQIDWAEAG
CCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHCCCCCCCCCCEEEEECCCCCEEEHHHCC
FGVAIIPEFAISTKQHLNSKVTPLTNFSSTSLGYIVRQNQVLSKATKQLLGWVNDEITLL
CCEEEECHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEE
QNK
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8045898; 9579061; 9384377; 8045899 [H]