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The map label for this gene is fdnI [H]

Identifier: 218709474

GI number: 218709474

Start: 1615060

End: 1616133

Strand: Reverse

Name: fdnI [H]

Synonym: VS_1483

Alternate gene names: 218709474

Gene position: 1616133-1615060 (Counterclockwise)

Preceding gene: 218709475

Following gene: 218709473

Centisome position: 48.98

GC content: 45.81

Gene sequence:

>1074_bases
ATGCTTACAATGTTTAAGCGTCTCTTCCTTGTTGTGCTGCCGATGTTGGCAGCACTAACAATGCTTTCTCCTATGAGTCA
TGCATCTGAGACGAACTCATCACAAACTCAATCGAGCTCTGCTCAACGAGAAATCACACAGCTTGCTGGTGCCGATTTTT
GGCGACAAGTAAGAAATGGTGAGGAAGGTTACACCACATCTCAATCAGCTGAGCACGGTGTGTTAATCAGTACTCCAGGT
CAAACTTGGTACATATTGAAAGAGAAGTGGATGTCACCAGCCGGTGCGGTCGCTATCTTTGGCAGTATTGCTTTTGTGAC
TTTGATGTACGTTGTGATTGGCCCGCTAATGCTAAGTGCACCGAGAACTGGCCGTAAGATCAAACGTTGGTCTCGACTTG
ATCGTGCATTGCACTGGAGCATGGCGTTTACCTTCTTAACACTGGCGTTCAGTGGTTTGATGTTGGTTTACGGTAAACAC
TTCTTGAAGCCTTATATCCCAACGGATCTGTGGGGTTTCATCGTTCTACTGGCTAAGCAATACCATAACTACATCGGCCC
AATTTTCTATGTGCTGTTGATGGCTGTTCTTATCAAGTGGTGGCGCAAGTCTATCTTCAAGATGGTCGATATCCAGTGGT
TCATGAAACTGGGTGGTATGGTAGGGAAACATAAAGGTTCTCATCCATCTGCAGAGTTTTCGAACGCGGGTGAAAAAGCA
CTATTTTGGCTGCTTATCGTTATGGGCAGTGTCGCAGCGATCAGTGGCTTGGTGTTAGATTTCCCAATCTTTGGCCAAAC
ACGACGTGATATGGAGCTTTCAAACCTAGTCCACATGTTGGCTGCTTTGATCCTTATCTGTGGTTTTGTGTTCCACATCT
ACATCGGCTTGTTCGGTATGGAAGGCGCACTAGAAGGCATGGTAACGGGCGAAGTCGATGAAACTTGGGCTAAAGAACAT
CATGACCTTTGGTACAAAGAAGTGATGGAACAAGAGAAAAACGGCGTTGAACAAAGCGCTAACGCAGCAACGGAGAAAAC
GGAAGGGGTGAATAAGAATGAACAAACCTCATAA

Upstream 100 bases:

>100_bases
TTCCGTCAGCGTGTAGTTGAACGTGGTGCGAAAGGTGCAGGTTGGACAGACGGCAACGACCTTTCTTACGATGCGACTAA
GAGCTAAGTAGGAGAGACAT

Downstream 100 bases:

>100_bases
GGGTATTTGGGTTGCTTACATTCTAAGCTGCTTTACACCATTGACTTGTCTTCTCTCTGGTGTGATTGCCATTATCTACG
CGGGCTATCGTTTAGATAAA

Product: formate dehydrogenase

Products: CO2; NADH

Alternate protein names: Anaerobic formate dehydrogenase cytochrome b556 subunit; Formate dehydrogenase-N subunit gamma; FDH-N subunit gamma [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MLTMFKRLFLVVLPMLAALTMLSPMSHASETNSSQTQSSSAQREITQLAGADFWRQVRNGEEGYTTSQSAEHGVLISTPG
QTWYILKEKWMSPAGAVAIFGSIAFVTLMYVVIGPLMLSAPRTGRKIKRWSRLDRALHWSMAFTFLTLAFSGLMLVYGKH
FLKPYIPTDLWGFIVLLAKQYHNYIGPIFYVLLMAVLIKWWRKSIFKMVDIQWFMKLGGMVGKHKGSHPSAEFSNAGEKA
LFWLLIVMGSVAAISGLVLDFPIFGQTRRDMELSNLVHMLAALILICGFVFHIYIGLFGMEGALEGMVTGEVDETWAKEH
HDLWYKEVMEQEKNGVEQSANAATEKTEGVNKNEQTS

Sequences:

>Translated_357_residues
MLTMFKRLFLVVLPMLAALTMLSPMSHASETNSSQTQSSSAQREITQLAGADFWRQVRNGEEGYTTSQSAEHGVLISTPG
QTWYILKEKWMSPAGAVAIFGSIAFVTLMYVVIGPLMLSAPRTGRKIKRWSRLDRALHWSMAFTFLTLAFSGLMLVYGKH
FLKPYIPTDLWGFIVLLAKQYHNYIGPIFYVLLMAVLIKWWRKSIFKMVDIQWFMKLGGMVGKHKGSHPSAEFSNAGEKA
LFWLLIVMGSVAAISGLVLDFPIFGQTRRDMELSNLVHMLAALILICGFVFHIYIGLFGMEGALEGMVTGEVDETWAKEH
HDLWYKEVMEQEKNGVEQSANAATEKTEGVNKNEQTS
>Mature_357_residues
MLTMFKRLFLVVLPMLAALTMLSPMSHASETNSSQTQSSSAQREITQLAGADFWRQVRNGEEGYTTSQSAEHGVLISTPG
QTWYILKEKWMSPAGAVAIFGSIAFVTLMYVVIGPLMLSAPRTGRKIKRWSRLDRALHWSMAFTFLTLAFSGLMLVYGKH
FLKPYIPTDLWGFIVLLAKQYHNYIGPIFYVLLMAVLIKWWRKSIFKMVDIQWFMKLGGMVGKHKGSHPSAEFSNAGEKA
LFWLLIVMGSVAAISGLVLDFPIFGQTRRDMELSNLVHMLAALILICGFVFHIYIGLFGMEGALEGMVTGEVDETWAKEH
HDLWYKEVMEQEKNGVEQSANAATEKTEGVNKNEQTS

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. Subunit gamma is the cytochrome b556(FDN) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787750, Length=232, Percent_Identity=27.1551724137931, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1790325, Length=217, Percent_Identity=26.2672811059908, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 40179; Mature: 40179

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTMFKRLFLVVLPMLAALTMLSPMSHASETNSSQTQSSSAQREITQLAGADFWRQVRNG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
EEGYTTSQSAEHGVLISTPGQTWYILKEKWMSPAGAVAIFGSIAFVTLMYVVIGPLMLSA
CCCCCCCCCCCCCEEEECCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PRTGRKIKRWSRLDRALHWSMAFTFLTLAFSGLMLVYGKHFLKPYIPTDLWGFIVLLAKQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
YHNYIGPIFYVLLMAVLIKWWRKSIFKMVDIQWFMKLGGMVGKHKGSHPSAEFSNAGEKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCHHCCCHHHH
LFWLLIVMGSVAAISGLVLDFPIFGQTRRDMELSNLVHMLAALILICGFVFHIYIGLFGM
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGALEGMVTGEVDETWAKEHHDLWYKEVMEQEKNGVEQSANAATEKTEGVNKNEQTS
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLTMFKRLFLVVLPMLAALTMLSPMSHASETNSSQTQSSSAQREITQLAGADFWRQVRNG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
EEGYTTSQSAEHGVLISTPGQTWYILKEKWMSPAGAVAIFGSIAFVTLMYVVIGPLMLSA
CCCCCCCCCCCCCEEEECCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PRTGRKIKRWSRLDRALHWSMAFTFLTLAFSGLMLVYGKHFLKPYIPTDLWGFIVLLAKQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
YHNYIGPIFYVLLMAVLIKWWRKSIFKMVDIQWFMKLGGMVGKHKGSHPSAEFSNAGEKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCHHCCCHHHH
LFWLLIVMGSVAAISGLVLDFPIFGQTRRDMELSNLVHMLAALILICGFVFHIYIGLFGM
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGALEGMVTGEVDETWAKEHHDLWYKEVMEQEKNGVEQSANAATEKTEGVNKNEQTS
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]