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The map label for this gene is fdhB1 [H]

Identifier: 218709475

GI number: 218709475

Start: 1616147

End: 1616755

Strand: Reverse

Name: fdhB1 [H]

Synonym: VS_1484

Alternate gene names: 218709475

Gene position: 1616755-1616147 (Counterclockwise)

Preceding gene: 218709476

Following gene: 218709474

Centisome position: 49.0

GC content: 47.95

Gene sequence:

>609_bases
ATGGCTAGAATGAAATTTCTTTGTGACACCAAACGTTGTATCGAATGTAACGGTTGTGTCACTGCATGTAAGAACGAAAA
TGATGATGCTCTGGAGTGGGGTATTCAACGCCGCCGCGTTGTGACACTGAACGATGGTGAACCGGGTGAAAACTCTATCT
CAGTCGCATGTATGCACTGTACTGATGCCCCTTGTATGGCAGTTTGCCCAGCAGACTGTTTTGAACATACAGAAGACGGC
ATCGTACTTCACAATAAAGATCTATGTATCGGTTGTGGTTACTGCTTGTTTGCTTGTCCGTTTGGCGCACCTCAATTCCC
TAAACAGGAAGCCTTTGGTGAGCGCGGTAAAATGGACAAATGTACCTTCTGTGCTGGCGGCCCTGAAACAGAACCTGGTT
CTGTGGAAGAGCGTCAGAAGTACGGTGCGAACCGTATTGCTGAAGGTAAGCTACCAATGTGTGCTTCGCTTTGTTCAACA
AAAGCGCTGCTTGCAGGTGATGCTGAGCAAGTCTCTGATGTTTTCCGTCAGCGTGTAGTTGAACGTGGTGCGAAAGGTGC
AGGTTGGACAGACGGCAACGACCTTTCTTACGATGCGACTAAGAGCTAA

Upstream 100 bases:

>100_bases
CTGCCAATACAGCAACCACATATGGTTACGACCCTGTCACGTTGATGCAGGAAACGAAAGTAACCCTTTGTAATATTCGT
AAAGCGTAAGGAGTCTTAAA

Downstream 100 bases:

>100_bases
GTAGGAGAGACATATGCTTACAATGTTTAAGCGTCTCTTCCTTGTTGTGCTGCCGATGTTGGCAGCACTAACAATGCTTT
CTCCTATGAGTCATGCATCT

Product: formate dehydrogenase iron-sulfur subunit

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MARMKFLCDTKRCIECNGCVTACKNENDDALEWGIQRRRVVTLNDGEPGENSISVACMHCTDAPCMAVCPADCFEHTEDG
IVLHNKDLCIGCGYCLFACPFGAPQFPKQEAFGERGKMDKCTFCAGGPETEPGSVEERQKYGANRIAEGKLPMCASLCST
KALLAGDAEQVSDVFRQRVVERGAKGAGWTDGNDLSYDATKS

Sequences:

>Translated_202_residues
MARMKFLCDTKRCIECNGCVTACKNENDDALEWGIQRRRVVTLNDGEPGENSISVACMHCTDAPCMAVCPADCFEHTEDG
IVLHNKDLCIGCGYCLFACPFGAPQFPKQEAFGERGKMDKCTFCAGGPETEPGSVEERQKYGANRIAEGKLPMCASLCST
KALLAGDAEQVSDVFRQRVVERGAKGAGWTDGNDLSYDATKS
>Mature_201_residues
ARMKFLCDTKRCIECNGCVTACKNENDDALEWGIQRRRVVTLNDGEPGENSISVACMHCTDAPCMAVCPADCFEHTEDGI
VLHNKDLCIGCGYCLFACPFGAPQFPKQEAFGERGKMDKCTFCAGGPETEPGSVEERQKYGANRIAEGKLPMCASLCSTK
ALLAGDAEQVSDVFRQRVVERGAKGAGWTDGNDLSYDATKS

Specific function: This chain is an electron transfer unit containing 18 cysteine residues, 16 of which occur in four clusters [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787872, Length=177, Percent_Identity=33.8983050847458, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI1787122, Length=177, Percent_Identity=32.7683615819209, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1789370, Length=198, Percent_Identity=27.7777777777778, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI226510944, Length=194, Percent_Identity=31.4432989690722, Blast_Score=80, Evalue=7e-17,
Organism=Escherichia coli, GI1790326, Length=208, Percent_Identity=30.2884615384615, Blast_Score=77, Evalue=6e-16,
Organism=Escherichia coli, GI1787749, Length=204, Percent_Identity=28.4313725490196, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI2367345, Length=199, Percent_Identity=30.1507537688442, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1788811, Length=133, Percent_Identity=31.5789473684211, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1789079, Length=200, Percent_Identity=29.5, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87082114, Length=91, Percent_Identity=37.3626373626374, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI2367245, Length=173, Percent_Identity=31.7919075144509, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR000813 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.1.2

Molecular weight: Translated: 21886; Mature: 21755

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
11.9 %Cys+Met (Translated Protein)
9.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
11.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARMKFLCDTKRCIECNGCVTACKNENDDALEWGIQRRRVVTLNDGEPGENSISVACMHC
CCCCCHHHCCCCEEECCCCEEECCCCCCHHHHHHHCCCEEEEECCCCCCCCCEEEEEEEC
TDAPCMAVCPADCFEHTEDGIVLHNKDLCIGCGYCLFACPFGAPQFPKQEAFGERGKMDK
CCCCCEEECCHHHHHCCCCCEEEECCCEEEECCHHHHCCCCCCCCCCCHHHHCCCCCCCC
CTFCAGGPETEPGSVEERQKYGANRIAEGKLPMCASLCSTKALLAGDAEQVSDVFRQRVV
EEEECCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
ERGAKGAGWTDGNDLSYDATKS
HCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ARMKFLCDTKRCIECNGCVTACKNENDDALEWGIQRRRVVTLNDGEPGENSISVACMHC
CCCCHHHCCCCEEECCCCEEECCCCCCHHHHHHHCCCEEEEECCCCCCCCCEEEEEEEC
TDAPCMAVCPADCFEHTEDGIVLHNKDLCIGCGYCLFACPFGAPQFPKQEAFGERGKMDK
CCCCCEEECCHHHHHCCCCCEEEECCCEEEECCHHHHCCCCCCCCCCCHHHHCCCCCCCC
CTFCAGGPETEPGSVEERQKYGANRIAEGKLPMCASLCSTKALLAGDAEQVSDVFRQRVV
EEEECCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
ERGAKGAGWTDGNDLSYDATKS
HCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1781728; 14500908 [H]