Click here to switch to the map view.

The map label for this gene is potD [H]

Identifier: 218709468

GI number: 218709468

Start: 1608663

End: 1609697

Strand: Direct

Name: potD [H]

Synonym: VS_1477

Alternate gene names: 218709468

Gene position: 1608663-1609697 (Clockwise)

Preceding gene: 218709467

Following gene: 218709469

Centisome position: 48.76

GC content: 44.64

Gene sequence:

>1035_bases
ATGAAAAAATGGGCTACTTTATTAGCTGGTAGTGCATGTGCGCTTTCAATGTTATCTGCACCATCATTTGCAAAAGATAA
CAAAGAATTGGTATTCATGAACTGGGGACCTTACATCAACAGTGAGATTCTAGAACAGTTCACTGATGAAACTGGTATCA
AGGTTATCTACTCGACTTACGAGTCGAATGAAACCTTGTACGCAAAGCTAAAAACACACAACAAAGGCTATGACCTAGTT
GTGCCGTCGACTTACTTCGTATCTAAGATGCGTGACGAAGGTATGCTACAAAAGATCGACAATACTAAGCTGAACAACTT
CAAGAATCTAGATACTAACTACCTAGATAAGCCGTACGATCCAAGCAACGACTACTCTATTCCACACGTCGTTGCAATCA
CAGGTCTTGCTGTTAACACTGACATGTACGACCCGGAAGATTTCCAAAGCTGGGCTGATCTATGGAAGCCAGAGCTTGAA
GGTCAACTTATGATGATGGACGACACTCGCGAAGTGTTCCACATCGCACTACGTAAGTTAGGTTATTCTGGTAATACAAC
AAACGAAAAAGAGATCGACGAGGCTTACGCTGAGCTGCGTAAGCTAATGCCGAACGTTCTAGTATTTAACTCAGACAACC
CAGGCGCGCCTTACATGTCTGGTGAAGTGGGTCTTGGTATGCTGTGGAACGGTTCTGCTGCTGCTGCGCAAAACGAAGGT
CTACCAATCAAACTGGTTTTCCCTAAAGAAGGCGGTATCGGTTGGGTTGATAACTTCGCGATTAGCTCTGGTGCGGTAAA
CGTAGAAGCAGCTCATAAGATGATCGACTTCCTACTTCGTCCTGAAATTGCTGAGCAAATCTCTCGTGACACCGGCTACC
TAACGGCTGTTAAAGCGTCTAACGAGAAGTTCAAAGACAGCCCTGCGCTGTTCCCATCTCAAGAAGACCTTGATCGTGTG
GAATGGCAAGCTGCGGTTGGCGATAAGACAGTGAAATACGAAGATTACTTCATGAAGCTTAAAGCCGGTCATTAA

Upstream 100 bases:

>100_bases
TCAAGTTCAAAAAAGTGTTAATCATAAACAGAATGGTAAATTGCCATTCTGTTTTTCTATCTACTGCCTTCGGGCAACGT
TTAGTTTGGAGCTAACGTCA

Downstream 100 bases:

>100_bases
GCGAGTTTGTTATCTAATTACTCCGGTCAACAGAGGCTAATAAGTAACGGGTTTACTTAACAAGCCTAGTTAGGTAATCA
ACAAGTTAAAAATAAGAATA

Product: spermidine/putrescine-binding periplasmic protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MKKWATLLAGSACALSMLSAPSFAKDNKELVFMNWGPYINSEILEQFTDETGIKVIYSTYESNETLYAKLKTHNKGYDLV
VPSTYFVSKMRDEGMLQKIDNTKLNNFKNLDTNYLDKPYDPSNDYSIPHVVAITGLAVNTDMYDPEDFQSWADLWKPELE
GQLMMMDDTREVFHIALRKLGYSGNTTNEKEIDEAYAELRKLMPNVLVFNSDNPGAPYMSGEVGLGMLWNGSAAAAQNEG
LPIKLVFPKEGGIGWVDNFAISSGAVNVEAAHKMIDFLLRPEIAEQISRDTGYLTAVKASNEKFKDSPALFPSQEDLDRV
EWQAAVGDKTVKYEDYFMKLKAGH

Sequences:

>Translated_344_residues
MKKWATLLAGSACALSMLSAPSFAKDNKELVFMNWGPYINSEILEQFTDETGIKVIYSTYESNETLYAKLKTHNKGYDLV
VPSTYFVSKMRDEGMLQKIDNTKLNNFKNLDTNYLDKPYDPSNDYSIPHVVAITGLAVNTDMYDPEDFQSWADLWKPELE
GQLMMMDDTREVFHIALRKLGYSGNTTNEKEIDEAYAELRKLMPNVLVFNSDNPGAPYMSGEVGLGMLWNGSAAAAQNEG
LPIKLVFPKEGGIGWVDNFAISSGAVNVEAAHKMIDFLLRPEIAEQISRDTGYLTAVKASNEKFKDSPALFPSQEDLDRV
EWQAAVGDKTVKYEDYFMKLKAGH
>Mature_344_residues
MKKWATLLAGSACALSMLSAPSFAKDNKELVFMNWGPYINSEILEQFTDETGIKVIYSTYESNETLYAKLKTHNKGYDLV
VPSTYFVSKMRDEGMLQKIDNTKLNNFKNLDTNYLDKPYDPSNDYSIPHVVAITGLAVNTDMYDPEDFQSWADLWKPELE
GQLMMMDDTREVFHIALRKLGYSGNTTNEKEIDEAYAELRKLMPNVLVFNSDNPGAPYMSGEVGLGMLWNGSAAAAQNEG
LPIKLVFPKEGGIGWVDNFAISSGAVNVEAAHKMIDFLLRPEIAEQISRDTGYLTAVKASNEKFKDSPALFPSQEDLDRV
EWQAAVGDKTVKYEDYFMKLKAGH

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=349, Percent_Identity=56.7335243553009, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1787078, Length=334, Percent_Identity=32.3353293413174, Blast_Score=159, Evalue=3e-40,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38619; Mature: 38619

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWATLLAGSACALSMLSAPSFAKDNKELVFMNWGPYINSEILEQFTDETGIKVIYSTY
CCHHHHHHCCCHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCEEEEEEE
ESNETLYAKLKTHNKGYDLVVPSTYFVSKMRDEGMLQKIDNTKLNNFKNLDTNYLDKPYD
CCCCEEEEEEEECCCCCEEEECCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCC
PSNDYSIPHVVAITGLAVNTDMYDPEDFQSWADLWKPELEGQLMMMDDTREVFHIALRKL
CCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHH
GYSGNTTNEKEIDEAYAELRKLMPNVLVFNSDNPGAPYMSGEVGLGMLWNGSAAAAQNEG
CCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEECCCCCEEEEECCCCCCCCCCC
LPIKLVFPKEGGIGWVDNFAISSGAVNVEAAHKMIDFLLRPEIAEQISRDTGYLTAVKAS
CEEEEEEECCCCCCCHHCEEECCCCEEHHHHHHHHHHHHCHHHHHHHCCCCCEEEEEECC
NEKFKDSPALFPSQEDLDRVEWQAAVGDKTVKYEDYFMKLKAGH
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEECCC
>Mature Secondary Structure
MKKWATLLAGSACALSMLSAPSFAKDNKELVFMNWGPYINSEILEQFTDETGIKVIYSTY
CCHHHHHHCCCHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCEEEEEEE
ESNETLYAKLKTHNKGYDLVVPSTYFVSKMRDEGMLQKIDNTKLNNFKNLDTNYLDKPYD
CCCCEEEEEEEECCCCCEEEECCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCC
PSNDYSIPHVVAITGLAVNTDMYDPEDFQSWADLWKPELEGQLMMMDDTREVFHIALRKL
CCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHH
GYSGNTTNEKEIDEAYAELRKLMPNVLVFNSDNPGAPYMSGEVGLGMLWNGSAAAAQNEG
CCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEECCCCCEEEEECCCCCCCCCCC
LPIKLVFPKEGGIGWVDNFAISSGAVNVEAAHKMIDFLLRPEIAEQISRDTGYLTAVKAS
CEEEEEEECCCCCCCHHCEEECCCCEEHHHHHHHHHHHHCHHHHHHHCCCCCEEEEEECC
NEKFKDSPALFPSQEDLDRVEWQAAVGDKTVKYEDYFMKLKAGH
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]