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The map label for this gene is potB [H]

Identifier: 218709466

GI number: 218709466

Start: 1606886

End: 1607743

Strand: Direct

Name: potB [H]

Synonym: VS_1475

Alternate gene names: 218709466

Gene position: 1606886-1607743 (Clockwise)

Preceding gene: 218709465

Following gene: 218709467

Centisome position: 48.7

GC content: 41.84

Gene sequence:

>858_bases
ATGAGCAAGAAGTTTAATTTACAAAACGCAATTGTTGCTTTAATCACAGGTTGGTTAGTGCTGTTCGTGATGATTCCAAA
CATCATGATCATCGGTACTAGTTTCTTAACTCGTGATGAAGCTAACTTGATTGAGATGACTTTTACTCTCGATAACTATG
TGCGTTTGGCTGATCCGCTGTATTTTAAAGTGCTGATGCACTCTTTCTATATGGCAATCGTAGCTACCCTGCTTTGTTTA
ATTATTGGTTACCCGTTCGCCTATATCGTGGCAAAAATGCCTGCGAAATGGCGTCCAATCATGTTGTTTTTAGTGATTGT
GCCATTTTGGACTAACTCACTGATTCGTACTTACGGATTAAAAGTGGTTCTGGGTACTCAAGGTGTGTTGAACAAAGGCT
TGTTGGCGTTAGACATTATCGATAAGCCACTTCGTATTATGTATTCAGAAACGGCAGTAATGATCGGTTTAGTGTATATC
CTACTCCCGTTCATGATTTTGCCGTTGTACTCAGCAATCGAAAAGCTAGACGACACTTATTTAGAAGCGGCTAAAGATTT
AGGTGCGAACAAACTGCAAACGCTATTGAAAGTCGTGTTACCACTGACTATGCCAGGTATTGTCGGCGGTTGTTTATTGG
TGTTATTACCTGCATTAGGTATGTTCTACATCTCTGACCTATTAGGCGGAGCAAAGAACCTGCTGATTGGTAACGTGATT
AAGAGCCAAGTACTCAATGCTCGGGACTGGCCGTTTGGCGCTGCGACGAGTATTGCACTGACCGTGGCAATGGCTGTGAT
GCTTTATGCCTACTACCGTGCAGGTAAGTTATTGAATAAGAAAGTGGAGCTAGACTAA

Upstream 100 bases:

>100_bases
GTTAGTGAATTCTTCAACGAAGATGACCCTGATGTTGATCACTCACTGGGCCAAAAAGTTGCAGTAACTTGGGTTGAGAG
CTGGGAAGTGGTATTAGAAG

Downstream 100 bases:

>100_bases
TGGGTCGCACAGTTAAGTTCAGCTTTATGGCGTTGGTATACGCTTTTCTATACCTACCTATTATCGTATTGATTGCAAAC
TCATTTAATGCCAATAAATT

Product: spermidine/putrescine ABC transporter membrane protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSKKFNLQNAIVALITGWLVLFVMIPNIMIIGTSFLTRDEANLIEMTFTLDNYVRLADPLYFKVLMHSFYMAIVATLLCL
IIGYPFAYIVAKMPAKWRPIMLFLVIVPFWTNSLIRTYGLKVVLGTQGVLNKGLLALDIIDKPLRIMYSETAVMIGLVYI
LLPFMILPLYSAIEKLDDTYLEAAKDLGANKLQTLLKVVLPLTMPGIVGGCLLVLLPALGMFYISDLLGGAKNLLIGNVI
KSQVLNARDWPFGAATSIALTVAMAVMLYAYYRAGKLLNKKVELD

Sequences:

>Translated_285_residues
MSKKFNLQNAIVALITGWLVLFVMIPNIMIIGTSFLTRDEANLIEMTFTLDNYVRLADPLYFKVLMHSFYMAIVATLLCL
IIGYPFAYIVAKMPAKWRPIMLFLVIVPFWTNSLIRTYGLKVVLGTQGVLNKGLLALDIIDKPLRIMYSETAVMIGLVYI
LLPFMILPLYSAIEKLDDTYLEAAKDLGANKLQTLLKVVLPLTMPGIVGGCLLVLLPALGMFYISDLLGGAKNLLIGNVI
KSQVLNARDWPFGAATSIALTVAMAVMLYAYYRAGKLLNKKVELD
>Mature_284_residues
SKKFNLQNAIVALITGWLVLFVMIPNIMIIGTSFLTRDEANLIEMTFTLDNYVRLADPLYFKVLMHSFYMAIVATLLCLI
IGYPFAYIVAKMPAKWRPIMLFLVIVPFWTNSLIRTYGLKVVLGTQGVLNKGLLALDIIDKPLRIMYSETAVMIGLVYIL
LPFMILPLYSAIEKLDDTYLEAAKDLGANKLQTLLKVVLPLTMPGIVGGCLLVLLPALGMFYISDLLGGAKNLLIGNVIK
SQVLNARDWPFGAATSIALTVAMAVMLYAYYRAGKLLNKKVELD

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=275, Percent_Identity=68.3636363636364, Blast_Score=402, Evalue=1e-114,
Organism=Escherichia coli, GI1787080, Length=234, Percent_Identity=42.3076923076923, Blast_Score=194, Evalue=6e-51,
Organism=Escherichia coli, GI1787713, Length=259, Percent_Identity=29.3436293436293, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1788764, Length=197, Percent_Identity=26.9035532994924, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31680; Mature: 31549

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKFNLQNAIVALITGWLVLFVMIPNIMIIGTSFLTRDEANLIEMTFTLDNYVRLADPL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEEEHHHHHHHHHHH
YFKVLMHSFYMAIVATLLCLIIGYPFAYIVAKMPAKWRPIMLFLVIVPFWTNSLIRTYGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
KVVLGTQGVLNKGLLALDIIDKPLRIMYSETAVMIGLVYILLPFMILPLYSAIEKLDDTY
EEEECCCHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEAAKDLGANKLQTLLKVVLPLTMPGIVGGCLLVLLPALGMFYISDLLGGAKNLLIGNVI
HHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KSQVLNARDWPFGAATSIALTVAMAVMLYAYYRAGKLLNKKVELD
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SKKFNLQNAIVALITGWLVLFVMIPNIMIIGTSFLTRDEANLIEMTFTLDNYVRLADPL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEEEHHHHHHHHHHH
YFKVLMHSFYMAIVATLLCLIIGYPFAYIVAKMPAKWRPIMLFLVIVPFWTNSLIRTYGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
KVVLGTQGVLNKGLLALDIIDKPLRIMYSETAVMIGLVYILLPFMILPLYSAIEKLDDTY
EEEECCCHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEAAKDLGANKLQTLLKVVLPLTMPGIVGGCLLVLLPALGMFYISDLLGGAKNLLIGNVI
HHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KSQVLNARDWPFGAATSIALTVAMAVMLYAYYRAGKLLNKKVELD
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]