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The map label for this gene is rluE [H]

Identifier: 218709063

GI number: 218709063

Start: 1125411

End: 1126139

Strand: Reverse

Name: rluE [H]

Synonym: VS_1069

Alternate gene names: 218709063

Gene position: 1126139-1125411 (Counterclockwise)

Preceding gene: 218709066

Following gene: 218709061

Centisome position: 34.13

GC content: 45.54

Gene sequence:

>729_bases
ATGTCTACTCGCTCACGCAGCGCATCTCGTTCAGACAAACCGGCTCGTTCTGGTACCACATTAAAACAAAGCGGTAATAG
ACAGAATCAACACAGTGGCAAAAATGACACTGGCAGTTCACACAAAAAACCGCACTCCAGCAAACACCGATATAAAGGCA
AAACTACCAACGCAAAGCCTAAGGTAGCACCCGAAGATCGCAAAGTGATTCTATTCAACAAACCTTTTGATACTCTCAGC
CAGTTCACTGATGGCGAAGGTAGAAAAACGCTAGCGGATTTTATTCCCGTAAAAGATGTTTATGCTGCTGGTCGCTTAGA
TCGAGATAGTGAAGGTTTATTGGTCTTGACCAATGATGGCATCTTTCAAGCTAAGTTGACTCAGCCGAACTCAAAATCGA
CCAAGACTTACTGGGTGCAGGTTGAAGGTGCGCCTTCTGAGAAAGATTTAGATAAATTGAGAAAAGGTGTTGAGCTAAAA
GATGGTATGACATTGCCTGCAGAAGTAGAAGTGATGCAAGAACCTCAGGTTTGGGAAAGAACTCCTCCTGTGCGCTTCAG
AGCGGCGATACCAACAACATGGTTAGCGATTACCATCATCGAAGGACGTAACCGTCAGGTAAGACGAATGACGGCAAACA
TAGGCTTCCCTACCCTGCGTCTAATCCGCTACTCAATGGGCAATATGAACGTGGGTCAGCTTCAGCCAGGTGAATGGAAA
GAAGTGTGA

Upstream 100 bases:

>100_bases
GTTCTATTTTGTGTCTTTTAGGCGCGCAAATGATAGCTCAATTCCGTTTCTATGAAAACTTTTCGGCACACTTCGTTTAC
ATTTTTGCAAGGTAGTTAAC

Downstream 100 bases:

>100_bases
AAACAACGACGAAATAACCAAATAAAATGGCCGCTGATTCAGTGGCCATTTTTATATGCAACGGGCAAATTCCAGATACA
AAAAAGCCAGTCATAAGACT

Product: ribosomal large subunit pseudouridine synthase E

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MSTRSRSASRSDKPARSGTTLKQSGNRQNQHSGKNDTGSSHKKPHSSKHRYKGKTTNAKPKVAPEDRKVILFNKPFDTLS
QFTDGEGRKTLADFIPVKDVYAAGRLDRDSEGLLVLTNDGIFQAKLTQPNSKSTKTYWVQVEGAPSEKDLDKLRKGVELK
DGMTLPAEVEVMQEPQVWERTPPVRFRAAIPTTWLAITIIEGRNRQVRRMTANIGFPTLRLIRYSMGNMNVGQLQPGEWK
EV

Sequences:

>Translated_242_residues
MSTRSRSASRSDKPARSGTTLKQSGNRQNQHSGKNDTGSSHKKPHSSKHRYKGKTTNAKPKVAPEDRKVILFNKPFDTLS
QFTDGEGRKTLADFIPVKDVYAAGRLDRDSEGLLVLTNDGIFQAKLTQPNSKSTKTYWVQVEGAPSEKDLDKLRKGVELK
DGMTLPAEVEVMQEPQVWERTPPVRFRAAIPTTWLAITIIEGRNRQVRRMTANIGFPTLRLIRYSMGNMNVGQLQPGEWK
EV
>Mature_241_residues
STRSRSASRSDKPARSGTTLKQSGNRQNQHSGKNDTGSSHKKPHSSKHRYKGKTTNAKPKVAPEDRKVILFNKPFDTLSQ
FTDGEGRKTLADFIPVKDVYAAGRLDRDSEGLLVLTNDGIFQAKLTQPNSKSTKTYWVQVEGAPSEKDLDKLRKGVELKD
GMTLPAEVEVMQEPQVWERTPPVRFRAAIPTTWLAITIIEGRNRQVRRMTANIGFPTLRLIRYSMGNMNVGQLQPGEWKE
V

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=199, Percent_Identity=60.3015075376884, Blast_Score=244, Evalue=3e-66,
Organism=Escherichia coli, GI1787524, Length=180, Percent_Identity=32.2222222222222, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI1790453, Length=179, Percent_Identity=30.1675977653631, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI1788510, Length=179, Percent_Identity=31.8435754189944, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 27190; Mature: 27059

Theoretical pI: Translated: 10.82; Mature: 10.82

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTRSRSASRSDKPARSGTTLKQSGNRQNQHSGKNDTGSSHKKPHSSKHRYKGKTTNAKP
CCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCC
KVAPEDRKVILFNKPFDTLSQFTDGEGRKTLADFIPVKDVYAAGRLDRDSEGLLVLTNDG
CCCCCCCEEEEEECCHHHHHHHCCCCCCHHHHHHCCHHHHHHHCCCCCCCCCEEEEECCC
IFQAKLTQPNSKSTKTYWVQVEGAPSEKDLDKLRKGVELKDGMTLPAEVEVMQEPQVWER
EEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCHHHEEECCCCHHCC
TPPVRFRAAIPTTWLAITIIEGRNRQVRRMTANIGFPTLRLIRYSMGNMNVGQLQPGEWK
CCCEEEEEECCCEEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCC
EV
CC
>Mature Secondary Structure 
STRSRSASRSDKPARSGTTLKQSGNRQNQHSGKNDTGSSHKKPHSSKHRYKGKTTNAKP
CCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCC
KVAPEDRKVILFNKPFDTLSQFTDGEGRKTLADFIPVKDVYAAGRLDRDSEGLLVLTNDG
CCCCCCCEEEEEECCHHHHHHHCCCCCCHHHHHHCCHHHHHHHCCCCCCCCCEEEEECCC
IFQAKLTQPNSKSTKTYWVQVEGAPSEKDLDKLRKGVELKDGMTLPAEVEVMQEPQVWER
EEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCHHHEEECCCCHHCC
TPPVRFRAAIPTTWLAITIIEGRNRQVRRMTANIGFPTLRLIRYSMGNMNVGQLQPGEWK
CCCEEEEEECCCEEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCC
EV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA