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The map label for this gene is cspD [H]

Identifier: 218709066

GI number: 218709066

Start: 1129058

End: 1129288

Strand: Reverse

Name: cspD [H]

Synonym: VS_1072

Alternate gene names: 218709066

Gene position: 1129288-1129058 (Counterclockwise)

Preceding gene: 218709069

Following gene: 218709063

Centisome position: 34.23

GC content: 43.72

Gene sequence:

>231_bases
ATGTATAGCATGGCTACAGGTACAGTAAAATGGTTTAACAATGCCAAAGGGTTTGGCTTTATTTGTCCAGAAGGTGAAGA
TGGCGATATTTTCGCGCACTACTCCACAATACAAATGGAAGGTTATCGAACCTTAAAGGCAGGTCAACAGGTCGATTATG
AAGTAGAAAGTGGACCTAAAGGCTCCCATGCTAGCTCCGTTGTTCCTGTAGAAGGCAGCGCCGTAAAATAG

Upstream 100 bases:

>100_bases
GATTCAAGCTATCAGCTGAGATTGTTTAGTTGATAGGAATAATATCAATCGAACTTACATTGATTTGATATAACAACGTT
CGTAACAAAATGCATGAGGG

Downstream 100 bases:

>100_bases
GCGATTGCCAAACATGTAGACCTCTATCAGTCTTAATAAACGACTGTCAGGTAAGCAGAACATTGAAACCGCTCATTTCA
TACCTGTAATACAGGCTTAA

Product: Cold shock-like protein cspD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 76; Mature: 76

Protein sequence:

>76_residues
MYSMATGTVKWFNNAKGFGFICPEGEDGDIFAHYSTIQMEGYRTLKAGQQVDYEVESGPKGSHASSVVPVEGSAVK

Sequences:

>Translated_76_residues
MYSMATGTVKWFNNAKGFGFICPEGEDGDIFAHYSTIQMEGYRTLKAGQQVDYEVESGPKGSHASSVVPVEGSAVK
>Mature_76_residues
MYSMATGTVKWFNNAKGFGFICPEGEDGDIFAHYSTIQMEGYRTLKAGQQVDYEVESGPKGSHASSVVPVEGSAVK

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=68, Percent_Identity=77.9411764705882, Blast_Score=116, Evalue=3e-28,
Organism=Escherichia coli, GI1786841, Length=68, Percent_Identity=48.5294117647059, Blast_Score=78, Evalue=9e-17,
Organism=Escherichia coli, GI1787839, Length=68, Percent_Identity=51.4705882352941, Blast_Score=76, Evalue=4e-16,
Organism=Escherichia coli, GI1789979, Length=70, Percent_Identity=48.5714285714286, Blast_Score=75, Evalue=6e-16,
Organism=Escherichia coli, GI1787834, Length=68, Percent_Identity=48.5294117647059, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI2367114, Length=70, Percent_Identity=44.2857142857143, Blast_Score=73, Evalue=4e-15,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=48.4375, Blast_Score=72, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012751
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 8195; Mature: 8195

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSMATGTVKWFNNAKGFGFICPEGEDGDIFAHYSTIQMEGYRTLKAGQQVDYEVESGPK
CCCCCCCEEEECCCCCCCEEECCCCCCCCEEEEEEEEEECCEEEECCCCEEEEEECCCCC
GSHASSVVPVEGSAVK
CCCCCEEEECCCCCCC
>Mature Secondary Structure
MYSMATGTVKWFNNAKGFGFICPEGEDGDIFAHYSTIQMEGYRTLKAGQQVDYEVESGPK
CCCCCCCEEEECCCCCCCEEECCCCCCCCEEEEEEEEEECCEEEECCCCEEEEEECCCCC
GSHASSVVPVEGSAVK
CCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]