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The map label for this gene is emrD [H]

Identifier: 218709055

GI number: 218709055

Start: 1114861

End: 1116306

Strand: Reverse

Name: emrD [H]

Synonym: VS_1061

Alternate gene names: 218709055

Gene position: 1116306-1114861 (Counterclockwise)

Preceding gene: 218709057

Following gene: 218709054

Centisome position: 33.83

GC content: 45.64

Gene sequence:

>1446_bases
TTGCAACATTTCAAGGAAGCCTATGACTCCTTTTTATTTTATAACCTCAATAAATTCGGATTAGTTTTTATAATCCAAGC
TATAGAATTTTATCTTGATCAACTTCACACTTTTTGGTTGCTAAGTAGACGCTCAATCTCTAATATCTCGCGCCTAGATT
CCCAGGTTACTTTACTTATTTGGCTCTCCTTAATGGAACGCCAAGGCTTTTTACGTCCTAATTTTTGGAGAGATACGCAA
ATGTCCGCCACGTTTCCATTAGCGAAACTTACCTTTTTAATCGCTATCCTGACTGCCGTGGGTCAAATGACTCAAACAAT
GTACGTGCCTTCTATCGGTCATATGGCGGGTGAGTTCTTGGTGTCAGCATCTTCGCTTCAAGCTGTGATGGCGTGCTACC
TGATCCCTTATGGTCTGTCGCAGTTTGCATACGGTCCACTTTCTGATCGCCTAGGTCGCAAACCAATCATCGTTGTCGGT
TTGATCATCTACATCATCGGTACTTTGGTAGCATTGTTTGCTCACGAATATGAATGGTTCTTAGCAGGTAGCTTTATACA
AGGTTTAGGCATCGGTTGTGGTGGTGCGATGTCTCGTACATTGACTCGCGACTGTTTTGAAGGCGCAGAACTGCACCGCG
CAAACAGCTTAATTAGCATGTGTGTGATTTTCTCACCATTGATGGCTCCTGTATTAGGTGGGTACTTAACAGAAGCTTTC
GGTTGGCGTTCTAGCTACTTGTTCCTGGCGCTGTTTGGTATCGCAGTGGTGATCACCATGATGACGAGCATGATGGAAAC
TCTGCCAAAAGAACGACGCAAACATGAATCGGTTGCCAACAGCTACAAGTTCGTTCTATCTGACAAACGTTTCCAAGGTT
TCTTATTGGTATTGGTCGCAACATTTGCAGGTGTAGCGGTATTTGAAGCTGCGGCAGGTGTATTACTTGGCGGAGTACTC
GGCTTGCCAGCAACCACAGTAAGCTTGTTGTTTGTATTACCCATTCCAGGTTACTTAGTCGGTGCTGGCCTATCCAGTTA
CATCGCACAACGTAGCAGTGAGCGCCGCGCTCTGAATGTTGGACTAGTTGCTATCTTGGTAGGGTCTACCGTGGTGTTGA
TACCAGGTCTATTTAGTCAAACAACAGCATTAACTTTGATCGGTGGTGCTACGATTTACTTTTTGGGCGCTGGTATCTTA
TTCCCGGCGGCAACAACAGGGGCGCTTTCGCCATTCCCGTACCACGCAGGTACTGGAGGTGCAATTTTGGGTGGTATGCA
AAATCTAGGTGCTGGCATCGCAACACTACTGGCCTCGTTCTTCCCAGCTCAAGACCAACTTCCGCTCGGTTGCTTGATGA
TTGCAATGTCATTCATCGCCATGCTTGGTTTACGTTGGGTTAATCGTAAACCTGACCATTCAAACGAAATGCCATTGGCT
ATCTAA

Upstream 100 bases:

>100_bases
TCTGTTATTGCTTTGATTTCAAGAGCTTTCAACTTGTCAGACTTAGGCATCTTTGTGCCTACGCTTCCGGTCATGAATAA
CGCAAATTGATTCAGAGTAA

Downstream 100 bases:

>100_bases
AGTAAACTCTACCTCAGCTCAAACAACGAAGAGGGACAGACTGAAAAGTGTGTCCCTCTTCGTTGCACGTTTCCCCCAGC
TCACATCTACCATCGATTCC

Product: multidrug resistance protein D

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MQHFKEAYDSFLFYNLNKFGLVFIIQAIEFYLDQLHTFWLLSRRSISNISRLDSQVTLLIWLSLMERQGFLRPNFWRDTQ
MSATFPLAKLTFLIAILTAVGQMTQTMYVPSIGHMAGEFLVSASSLQAVMACYLIPYGLSQFAYGPLSDRLGRKPIIVVG
LIIYIIGTLVALFAHEYEWFLAGSFIQGLGIGCGGAMSRTLTRDCFEGAELHRANSLISMCVIFSPLMAPVLGGYLTEAF
GWRSSYLFLALFGIAVVITMMTSMMETLPKERRKHESVANSYKFVLSDKRFQGFLLVLVATFAGVAVFEAAAGVLLGGVL
GLPATTVSLLFVLPIPGYLVGAGLSSYIAQRSSERRALNVGLVAILVGSTVVLIPGLFSQTTALTLIGGATIYFLGAGIL
FPAATTGALSPFPYHAGTGGAILGGMQNLGAGIATLLASFFPAQDQLPLGCLMIAMSFIAMLGLRWVNRKPDHSNEMPLA
I

Sequences:

>Translated_481_residues
MQHFKEAYDSFLFYNLNKFGLVFIIQAIEFYLDQLHTFWLLSRRSISNISRLDSQVTLLIWLSLMERQGFLRPNFWRDTQ
MSATFPLAKLTFLIAILTAVGQMTQTMYVPSIGHMAGEFLVSASSLQAVMACYLIPYGLSQFAYGPLSDRLGRKPIIVVG
LIIYIIGTLVALFAHEYEWFLAGSFIQGLGIGCGGAMSRTLTRDCFEGAELHRANSLISMCVIFSPLMAPVLGGYLTEAF
GWRSSYLFLALFGIAVVITMMTSMMETLPKERRKHESVANSYKFVLSDKRFQGFLLVLVATFAGVAVFEAAAGVLLGGVL
GLPATTVSLLFVLPIPGYLVGAGLSSYIAQRSSERRALNVGLVAILVGSTVVLIPGLFSQTTALTLIGGATIYFLGAGIL
FPAATTGALSPFPYHAGTGGAILGGMQNLGAGIATLLASFFPAQDQLPLGCLMIAMSFIAMLGLRWVNRKPDHSNEMPLA
I
>Mature_481_residues
MQHFKEAYDSFLFYNLNKFGLVFIIQAIEFYLDQLHTFWLLSRRSISNISRLDSQVTLLIWLSLMERQGFLRPNFWRDTQ
MSATFPLAKLTFLIAILTAVGQMTQTMYVPSIGHMAGEFLVSASSLQAVMACYLIPYGLSQFAYGPLSDRLGRKPIIVVG
LIIYIIGTLVALFAHEYEWFLAGSFIQGLGIGCGGAMSRTLTRDCFEGAELHRANSLISMCVIFSPLMAPVLGGYLTEAF
GWRSSYLFLALFGIAVVITMMTSMMETLPKERRKHESVANSYKFVLSDKRFQGFLLVLVATFAGVAVFEAAAGVLLGGVL
GLPATTVSLLFVLPIPGYLVGAGLSSYIAQRSSERRALNVGLVAILVGSTVVLIPGLFSQTTALTLIGGATIYFLGAGIL
FPAATTGALSPFPYHAGTGGAILGGMQNLGAGIATLLASFFPAQDQLPLGCLMIAMSFIAMLGLRWVNRKPDHSNEMPLA
I

Specific function: Multidrug resistance pump that participates in a low energy shock adaptative response [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI87082312, Length=348, Percent_Identity=42.5287356321839, Blast_Score=265, Evalue=7e-72,
Organism=Escherichia coli, GI48994889, Length=342, Percent_Identity=24.2690058479532, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788509, Length=354, Percent_Identity=24.0112994350282, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1790146, Length=378, Percent_Identity=26.1904761904762, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787065, Length=303, Percent_Identity=25.7425742574257, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1790794, Length=337, Percent_Identity=23.1454005934718, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52181; Mature: 52181

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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C
>Mature Secondary Structure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C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8240355; 7686882; 9278503 [H]