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The map label for this gene is pflB [H]

Identifier: 218709046

GI number: 218709046

Start: 1100345

End: 1102621

Strand: Reverse

Name: pflB [H]

Synonym: VS_1052

Alternate gene names: 218709046

Gene position: 1102621-1100345 (Counterclockwise)

Preceding gene: 218709047

Following gene: 218709045

Centisome position: 33.42

GC content: 45.5

Gene sequence:

>2277_bases
ATGGCAGAGCAATTTGCTAAAGCTTGGGAAGATTTTGCTGCAGGTGAGTGGCAAAGCGAAGTAAACGTTCGTGATTTCAT
TCAAAAGAACTACACGCCATATGAAGGCGACGAGTCTTTCCTAGTTTCTGAGGGTACTGAAGCAACTAACAAGCTTTGGT
CTTCGGTAATGGAAGGTATCAAACAGGAAAACGCAACTAAAGCACCTGTAGATTTCGATACTTCTGTTATCTCTACCATT
ACTGCTCACGATGCAGGTTACATTGAGAAAGATCTTGAGACCATCGTTGGTCTACAAACTGAGAAGCCACTAAAACGTGC
AATCATCCCTAACGGTGGTGTACGTATGGTTGAAGGTTCTTGTAAAGCATACGGTGAAACTCTTGACCCAATGGTTTCAA
AAATCTACTCAGAATACCGCAAAACACACAATGCTGGCGTTTTCGATATCTACACTCCTGATATACTAAAATGTCGTAAG
TCTGGTGTTCTGACTGGTCTTCCTGATGCTTACGGCCGTGGTCGTATCATTGGTGACTACCGTCGTGTTGCACTTTACGG
TATCAACTTCCTAATGAAAGACAAAGCTGCTCAATTCGCTTCTCTACAAGAGCGTTTCGAGAACGGCGAAGATCTTGCTG
CTACAATGCAATTGCGTGAAGAGATCTCTGAGCAACATCGTGCTCTAGGTCAAATCAAGCAAATGGCTGAGAAATACGGT
TTCGATATCTCTGAGCCAGCTCAAACTGCTCAAGAAGCTATTCAGTGGACTTACTTCGGCTACCTAGCTGCTGTTAAATC
TCAAAACGGTGCTGCTATGTCTCTAGGTCGTACTTCGACTTTCCTAGACATCTACATCGAGCGTGATATCGCTGCTGGCA
AAATCACAGAAGACCAAGCACAAGAGATGATCGACCACTTCGTAATGAAGCTGCGTATGGTTCGTTTCCTACGTACTCCT
GAGTACGATGAGCTATTCTCTGGCGACCCAATCTGGGCTACAGAATCTATGGGTGGTATGGGTGTTGATGGTCGTACGCT
AGTAACGCGTTCGAACTTCCGTTTCCTTAACTCTCTATACACTATGGGTCCTTCTCCAGAGCCAAACATCACTGTTCTTT
GGTCTGAGCAACTCCCTGACGGCTTCAAGCGTTTCTGTGCGAAGGTATCTATCGATACTTCTTCTATCCAGTACGAAAAT
GATGACCTAATGCGTCCTGACCTTGGTTCTGATGATTACGCAATCGCTTGTTGTGTATCACCAATGATCGTTGGTAAGCA
AATGCAGTTCTTCGGCGCTCGTGCTAACCTTGCAAAAACTATGCTTTACGCAATCAACGGCGGCGTTGATGAGAAGCTTA
AAATGCAAGTTGGCCCAGTTGGCGACAAGATCACTGACGAAGTACTTAACTACGATGACGTAATGGGTCGCCTAGACACA
TTCATGGATTGGTTAGCTAAGCAATACGTGACAGCTCTAAACAGCATTCACTTCATGCACGACAAGTACAGCTACGAAGC
GTCTCTAATGGCTCTTCATGACCGTGACGTTCGTCGTACTATGGCTTGTGGTATCGCTGGTCTGTCTGTTGCAGCTGACT
CACTGTCTGCAATCAAATTCGCGACTGTTAAACCAATCCGCGACGAAGATGGCATTGCAACTGACTTCGAAATCGAAGGC
GATTACCCTAAATACGGTAACAACGACTCTCGTGTAGATGACATTGCTTGTGAACTAGTTTCTACGTTCATGAACAAGAT
CCGTAAGCTTAAGACTTACCGTGATTCAATCCCTACACAGTCAGTTCTTACTATCACGTCTAACGTGGTATACGGTAAGA
AAACAGGTAACACTCCAGACGGTCGTCGTGCTGGCGCTCCTTTCGCTCCTGGTGCAAACCCAATGCACGGTCGTGATGAG
AAAGGCGCTGTAGCTTCACTAACGTCTGTAGGTAAACTACCGTTTGCTGATGCTCAAGATGGTATCTCTTACACTTTCTC
TATCGTGCCAAACGCACTAGGTAAAGAAGAAGATAGCCAACGTGCTAACCTTGCAGGCCTAATGGATGGTTACTTCCACC
ACGAAGCTGGCATTGAAGGTGGTCAACACCTTAACGTGAACGTTCTTAACCGTGAAACTCTAGAAGACGCAGTTAAGCAC
CCTGAGAAATACCCTCAGCTAACGATTCGTGTTTCTGGTTACGCTGTACGCTTTAACTCTCTGACTACAGAGCAGCAAGC
TGACGTAATCGCACGTACATTTACTGAGTCTCTATAA

Upstream 100 bases:

>100_bases
CATATATATTGTTATTAATAAGAGTGTACTACCCTTACGAGGGGCGCTGGCTCAACAGTGTTAATGTACGTATTTTTTCT
ACTAAAAAGGTAGGTATGTC

Downstream 100 bases:

>100_bases
GCTCACGCTCAATAGACAGTAAAAACTATTAGCCTCGCCAATGTGCGGGGCTTTTTTATATCTACTCTAAATCTCATCAA
TACCCCCTCCCCCAAGAAAG

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 1 [H]

Number of amino acids: Translated: 758; Mature: 757

Protein sequence:

>758_residues
MAEQFAKAWEDFAAGEWQSEVNVRDFIQKNYTPYEGDESFLVSEGTEATNKLWSSVMEGIKQENATKAPVDFDTSVISTI
TAHDAGYIEKDLETIVGLQTEKPLKRAIIPNGGVRMVEGSCKAYGETLDPMVSKIYSEYRKTHNAGVFDIYTPDILKCRK
SGVLTGLPDAYGRGRIIGDYRRVALYGINFLMKDKAAQFASLQERFENGEDLAATMQLREEISEQHRALGQIKQMAEKYG
FDISEPAQTAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSTFLDIYIERDIAAGKITEDQAQEMIDHFVMKLRMVRFLRTP
EYDELFSGDPIWATESMGGMGVDGRTLVTRSNFRFLNSLYTMGPSPEPNITVLWSEQLPDGFKRFCAKVSIDTSSIQYEN
DDLMRPDLGSDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPVGDKITDEVLNYDDVMGRLDT
FMDWLAKQYVTALNSIHFMHDKYSYEASLMALHDRDVRRTMACGIAGLSVAADSLSAIKFATVKPIRDEDGIATDFEIEG
DYPKYGNNDSRVDDIACELVSTFMNKIRKLKTYRDSIPTQSVLTITSNVVYGKKTGNTPDGRRAGAPFAPGANPMHGRDE
KGAVASLTSVGKLPFADAQDGISYTFSIVPNALGKEEDSQRANLAGLMDGYFHHEAGIEGGQHLNVNVLNRETLEDAVKH
PEKYPQLTIRVSGYAVRFNSLTTEQQADVIARTFTESL

Sequences:

>Translated_758_residues
MAEQFAKAWEDFAAGEWQSEVNVRDFIQKNYTPYEGDESFLVSEGTEATNKLWSSVMEGIKQENATKAPVDFDTSVISTI
TAHDAGYIEKDLETIVGLQTEKPLKRAIIPNGGVRMVEGSCKAYGETLDPMVSKIYSEYRKTHNAGVFDIYTPDILKCRK
SGVLTGLPDAYGRGRIIGDYRRVALYGINFLMKDKAAQFASLQERFENGEDLAATMQLREEISEQHRALGQIKQMAEKYG
FDISEPAQTAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSTFLDIYIERDIAAGKITEDQAQEMIDHFVMKLRMVRFLRTP
EYDELFSGDPIWATESMGGMGVDGRTLVTRSNFRFLNSLYTMGPSPEPNITVLWSEQLPDGFKRFCAKVSIDTSSIQYEN
DDLMRPDLGSDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPVGDKITDEVLNYDDVMGRLDT
FMDWLAKQYVTALNSIHFMHDKYSYEASLMALHDRDVRRTMACGIAGLSVAADSLSAIKFATVKPIRDEDGIATDFEIEG
DYPKYGNNDSRVDDIACELVSTFMNKIRKLKTYRDSIPTQSVLTITSNVVYGKKTGNTPDGRRAGAPFAPGANPMHGRDE
KGAVASLTSVGKLPFADAQDGISYTFSIVPNALGKEEDSQRANLAGLMDGYFHHEAGIEGGQHLNVNVLNRETLEDAVKH
PEKYPQLTIRVSGYAVRFNSLTTEQQADVIARTFTESL
>Mature_757_residues
AEQFAKAWEDFAAGEWQSEVNVRDFIQKNYTPYEGDESFLVSEGTEATNKLWSSVMEGIKQENATKAPVDFDTSVISTIT
AHDAGYIEKDLETIVGLQTEKPLKRAIIPNGGVRMVEGSCKAYGETLDPMVSKIYSEYRKTHNAGVFDIYTPDILKCRKS
GVLTGLPDAYGRGRIIGDYRRVALYGINFLMKDKAAQFASLQERFENGEDLAATMQLREEISEQHRALGQIKQMAEKYGF
DISEPAQTAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSTFLDIYIERDIAAGKITEDQAQEMIDHFVMKLRMVRFLRTPE
YDELFSGDPIWATESMGGMGVDGRTLVTRSNFRFLNSLYTMGPSPEPNITVLWSEQLPDGFKRFCAKVSIDTSSIQYEND
DLMRPDLGSDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPVGDKITDEVLNYDDVMGRLDTF
MDWLAKQYVTALNSIHFMHDKYSYEASLMALHDRDVRRTMACGIAGLSVAADSLSAIKFATVKPIRDEDGIATDFEIEGD
YPKYGNNDSRVDDIACELVSTFMNKIRKLKTYRDSIPTQSVLTITSNVVYGKKTGNTPDGRRAGAPFAPGANPMHGRDEK
GAVASLTSVGKLPFADAQDGISYTFSIVPNALGKEEDSQRANLAGLMDGYFHHEAGIEGGQHLNVNVLNRETLEDAVKHP
EKYPQLTIRVSGYAVRFNSLTTEQQADVIARTFTESL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=758, Percent_Identity=80.7387862796834, Blast_Score=1310, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=754, Percent_Identity=72.9442970822281, Blast_Score=1196, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=624, Percent_Identity=28.3653846153846, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1790388, Length=632, Percent_Identity=24.8417721518987, Blast_Score=137, Evalue=3e-33,
Organism=Escherichia coli, GI1788933, Length=64, Percent_Identity=90.625, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 84243; Mature: 84112

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQFAKAWEDFAAGEWQSEVNVRDFIQKNYTPYEGDESFLVSEGTEATNKLWSSVMEGI
CCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHH
KQENATKAPVDFDTSVISTITAHDAGYIEKDLETIVGLQTEKPLKRAIIPNGGVRMVEGS
HHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEECC
CKAYGETLDPMVSKIYSEYRKTHNAGVFDIYTPDILKCRKSGVLTGLPDAYGRGRIIGDY
HHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCCCCCCCCCCCCEEHHH
RRVALYGINFLMKDKAAQFASLQERFENGEDLAATMQLREEISEQHRALGQIKQMAEKYG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FDISEPAQTAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSTFLDIYIERDIAAGKITEDQA
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEECCCCCCCCCHHHH
QEMIDHFVMKLRMVRFLRTPEYDELFSGDPIWATESMGGMGVDGRTLVTRSNFRFLNSLY
HHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEECCCCCCCCCCCEEEEECCHHHHHHHH
TMGPSPEPNITVLWSEQLPDGFKRFCAKVSIDTSSIQYENDDLMRPDLGSDDYAIACCVS
HCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEEEEHH
PMIVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPVGDKITDEVLNYDDVMGRLDT
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHCHHHHHHHHHH
FMDWLAKQYVTALNSIHFMHDKYSYEASLMALHDRDVRRTMACGIAGLSVAADSLSAIKF
HHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
ATVKPIRDEDGIATDFEIEGDYPKYGNNDSRVDDIACELVSTFMNKIRKLKTYRDSIPTQ
EEECCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SVLTITSNVVYGKKTGNTPDGRRAGAPFAPGANPMHGRDEKGAVASLTSVGKLPFADAQD
CEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC
GISYTFSIVPNALGKEEDSQRANLAGLMDGYFHHEAGIEGGQHLNVNVLNRETLEDAVKH
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHC
PEKYPQLTIRVSGYAVRFNSLTTEQQADVIARTFTESL
CCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEQFAKAWEDFAAGEWQSEVNVRDFIQKNYTPYEGDESFLVSEGTEATNKLWSSVMEGI
CHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHH
KQENATKAPVDFDTSVISTITAHDAGYIEKDLETIVGLQTEKPLKRAIIPNGGVRMVEGS
HHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEECC
CKAYGETLDPMVSKIYSEYRKTHNAGVFDIYTPDILKCRKSGVLTGLPDAYGRGRIIGDY
HHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCCCCCCCCCCCCEEHHH
RRVALYGINFLMKDKAAQFASLQERFENGEDLAATMQLREEISEQHRALGQIKQMAEKYG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FDISEPAQTAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSTFLDIYIERDIAAGKITEDQA
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEECCCCCCCCCHHHH
QEMIDHFVMKLRMVRFLRTPEYDELFSGDPIWATESMGGMGVDGRTLVTRSNFRFLNSLY
HHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEECCCCCCCCCCCEEEEECCHHHHHHHH
TMGPSPEPNITVLWSEQLPDGFKRFCAKVSIDTSSIQYENDDLMRPDLGSDDYAIACCVS
HCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEEEEHH
PMIVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPVGDKITDEVLNYDDVMGRLDT
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHCHHHHHHHHHH
FMDWLAKQYVTALNSIHFMHDKYSYEASLMALHDRDVRRTMACGIAGLSVAADSLSAIKF
HHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
ATVKPIRDEDGIATDFEIEGDYPKYGNNDSRVDDIACELVSTFMNKIRKLKTYRDSIPTQ
EEECCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SVLTITSNVVYGKKTGNTPDGRRAGAPFAPGANPMHGRDEKGAVASLTSVGKLPFADAQD
CEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC
GISYTFSIVPNALGKEEDSQRANLAGLMDGYFHHEAGIEGGQHLNVNVLNRETLEDAVKH
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHC
PEKYPQLTIRVSGYAVRFNSLTTEQQADVIARTFTESL
CCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3053170; 8905232; 9278503; 2651404; 1310545; 9629924; 10504733 [H]