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The map label for this gene is metG [H]

Identifier: 218709003

GI number: 218709003

Start: 1046063

End: 1048123

Strand: Reverse

Name: metG [H]

Synonym: VS_1009

Alternate gene names: 218709003

Gene position: 1048123-1046063 (Counterclockwise)

Preceding gene: 218709007

Following gene: 218709002

Centisome position: 31.77

GC content: 45.27

Gene sequence:

>2061_bases
ATGGCAACTGATCCAAGACAACTTTTGGTAACTTGTGCGCTTCCGTACGCTAACGGCTCTATTCACCTTGGCCATATGCT
TGAGCATATCCAAGCTGATATCTGGGTTCGATACCAGCGTCTACGTGGCAACACTGTAAACTTCATCTGTGCTGACGATG
CTCACGGCACGCCAATTATGCTTAAAGCTCAACAGATGGGTATCACACCAGAAGAGATGATCGCTGCTGTTAGTGAAGAG
CATCAAAAAGACTTCGCTGGCTTTGATATCAGCTTTGATAACTACCACAGCACACATAGTGAAGAGAACCGTGAACTGGC
TTCTCATATCTACCTTGAGCTTAAAAAGAACGGTTTCATCTCTAGCCGTACTATTTCTCAGCTTTTCGATCCTGAGAAAG
AGATGTTTCTTCCAGACCGTTTTGTAAAAGGTACTTGCCCTAAGTGTAAGTCAGAAGACCAATACGGTGATAACTGTGAT
AACTGTGGTGAGACATACAGCCCAACTGAACTGATTAATCCAAAATCAGCAGTTTCTGGCGCAACTCCAGTAATGAAAGA
CTCAGAACACTTCTTCTTCGATCTACCTCAGTTCGAAAGCATGCTTAAAGAGTGGACTCGTTCTGGATCTCTACAAAATG
AAACTGCAAACAAAATGCAGGAATGGTTTGAGTCTGGCCTGCAACAGTGGGATATCTCACGTGATGCGCCTTACTTCGGC
TTCGAAATCCCAGGCGAAAAAGACAAGTTCTTCTACGTATGGTTAGATGCGCCAGTTGGCTACATGGCTTCTTTCAAGAA
CCTATGTGACAAGCGTGACGATCTAAACTTCGACGAATACTGGAAGAAAGATAGCACAACTGAGCTTTACCACTTCATCG
GTAAAGACATTGTGTACTTCCACTCTCTATTCTGGCCGGCAATGCTAGAAGGCGCTGGTTTCCGTAAGCCAAACAACGTA
TTCGTACACGGCTACGTAACGGTGAACGGTGCGAAGATGTCTAAGTCGAAAGGCACATTCATCAAAGCAAGCACGTACTT
AAACCACCTAGACCCTGAGTGTCTTCGCTACTACTACGCTGCGAAACTAAACAGCCGTATCGATGACCTTGACCTTAACC
TTGAAGACTTCACTCAACGTGTAAACGCTGATGTAGTAAACAAGATTGTTAACCTAGCGTCTCGTAACGCTGGCTTCATC
ACTAAGCGTTTCGAAGGCAAGCTTTCTGCTGAATTTGCAGAACCTGAGCTATACAACGAATTCGTTGCTGCTGCAGAGCG
TATCGGTCAGCTATACGAAACTCGTGAGTTCAGCCGCGCTATCCGTGAAATTACTGCACTAGCTGATAAAGCTAACCAGT
ACATCGACGAAAAAGCACCTTGGGTTCTTGCAAAAGAAGAAGGTAAAGAGAAAGAGCTTCAAGAAGTTTCTTCTGTCGGT
ATTAACCTATTCCGCGTGCTAATGGCTTACCTTAAACCCGTTATGCCAGAGCTTGCGGCTCGCACTGAGGCTTTCCTAAA
CGAAGAGCTTACGTGGGAAGCGATTGCTACTCCGCTAACTGATCACGAAATCACTAAGTTCAAGGCGTTGTTTAGCCGTA
TTGATCCGAAGAAAGTAGAAGCAATGATCGAGTCTTCTAAAGAAGATGCAGCCGCTGAAGCAGCAGCAAAAGAAAAAGCA
GAAGCTGAAAAAGAGCAAGCAAGCCAAACTGAGCTAGACAAAGAGCCAATCGCAGACGAGATTGAATTCGATGCCTTTTC
AGCAGTCGATATGCGTATTGCTCGTATTATCTCATGTGAAGAAGTACCAAAAGCGAACAAGCTACTGAAGTTCCAACTGG
ACATCGGTGGTGAAACTCGCCAAGTATTCTCTGGTATCAAGTCAGCGTACAAACCTGAAGAGCTAGAAGGCAAACTAACT
GTGATGGTAGCAAACCTTAAACCTCGTAAGATGAAGTTTGGTATGTCTGAAGGCATGATCCTAGCTGCTGGCCCAGGTGG
CAGTGACTTGTGGATCCTTGAACCACACGAAGGTGCTCAACCTGGCATGCGTGTAATGTAG

Upstream 100 bases:

>100_bases
AACTAGGGGAATCCTTTGTCTGATAACTTGGCGTATCACACGTTATAAAGTAGTATTACCTCTCAAAATTTATACCTATC
AAAAAAGCGAATTATTAAGT

Downstream 100 bases:

>100_bases
CTCTGGCTTCAGTCAGATAACAAGCACTCATAGCGAAATCCCTAATGCACTTTTAACTTGAATGAGTTGATAAGCATTGG
GGATTTTTTATATCTACCGA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 686; Mature: 685

Protein sequence:

>686_residues
MATDPRQLLVTCALPYANGSIHLGHMLEHIQADIWVRYQRLRGNTVNFICADDAHGTPIMLKAQQMGITPEEMIAAVSEE
HQKDFAGFDISFDNYHSTHSEENRELASHIYLELKKNGFISSRTISQLFDPEKEMFLPDRFVKGTCPKCKSEDQYGDNCD
NCGETYSPTELINPKSAVSGATPVMKDSEHFFFDLPQFESMLKEWTRSGSLQNETANKMQEWFESGLQQWDISRDAPYFG
FEIPGEKDKFFYVWLDAPVGYMASFKNLCDKRDDLNFDEYWKKDSTTELYHFIGKDIVYFHSLFWPAMLEGAGFRKPNNV
FVHGYVTVNGAKMSKSKGTFIKASTYLNHLDPECLRYYYAAKLNSRIDDLDLNLEDFTQRVNADVVNKIVNLASRNAGFI
TKRFEGKLSAEFAEPELYNEFVAAAERIGQLYETREFSRAIREITALADKANQYIDEKAPWVLAKEEGKEKELQEVSSVG
INLFRVLMAYLKPVMPELAARTEAFLNEELTWEAIATPLTDHEITKFKALFSRIDPKKVEAMIESSKEDAAAEAAAKEKA
EAEKEQASQTELDKEPIADEIEFDAFSAVDMRIARIISCEEVPKANKLLKFQLDIGGETRQVFSGIKSAYKPEELEGKLT
VMVANLKPRKMKFGMSEGMILAAGPGGSDLWILEPHEGAQPGMRVM

Sequences:

>Translated_686_residues
MATDPRQLLVTCALPYANGSIHLGHMLEHIQADIWVRYQRLRGNTVNFICADDAHGTPIMLKAQQMGITPEEMIAAVSEE
HQKDFAGFDISFDNYHSTHSEENRELASHIYLELKKNGFISSRTISQLFDPEKEMFLPDRFVKGTCPKCKSEDQYGDNCD
NCGETYSPTELINPKSAVSGATPVMKDSEHFFFDLPQFESMLKEWTRSGSLQNETANKMQEWFESGLQQWDISRDAPYFG
FEIPGEKDKFFYVWLDAPVGYMASFKNLCDKRDDLNFDEYWKKDSTTELYHFIGKDIVYFHSLFWPAMLEGAGFRKPNNV
FVHGYVTVNGAKMSKSKGTFIKASTYLNHLDPECLRYYYAAKLNSRIDDLDLNLEDFTQRVNADVVNKIVNLASRNAGFI
TKRFEGKLSAEFAEPELYNEFVAAAERIGQLYETREFSRAIREITALADKANQYIDEKAPWVLAKEEGKEKELQEVSSVG
INLFRVLMAYLKPVMPELAARTEAFLNEELTWEAIATPLTDHEITKFKALFSRIDPKKVEAMIESSKEDAAAEAAAKEKA
EAEKEQASQTELDKEPIADEIEFDAFSAVDMRIARIISCEEVPKANKLLKFQLDIGGETRQVFSGIKSAYKPEELEGKLT
VMVANLKPRKMKFGMSEGMILAAGPGGSDLWILEPHEGAQPGMRVM
>Mature_685_residues
ATDPRQLLVTCALPYANGSIHLGHMLEHIQADIWVRYQRLRGNTVNFICADDAHGTPIMLKAQQMGITPEEMIAAVSEEH
QKDFAGFDISFDNYHSTHSEENRELASHIYLELKKNGFISSRTISQLFDPEKEMFLPDRFVKGTCPKCKSEDQYGDNCDN
CGETYSPTELINPKSAVSGATPVMKDSEHFFFDLPQFESMLKEWTRSGSLQNETANKMQEWFESGLQQWDISRDAPYFGF
EIPGEKDKFFYVWLDAPVGYMASFKNLCDKRDDLNFDEYWKKDSTTELYHFIGKDIVYFHSLFWPAMLEGAGFRKPNNVF
VHGYVTVNGAKMSKSKGTFIKASTYLNHLDPECLRYYYAAKLNSRIDDLDLNLEDFTQRVNADVVNKIVNLASRNAGFIT
KRFEGKLSAEFAEPELYNEFVAAAERIGQLYETREFSRAIREITALADKANQYIDEKAPWVLAKEEGKEKELQEVSSVGI
NLFRVLMAYLKPVMPELAARTEAFLNEELTWEAIATPLTDHEITKFKALFSRIDPKKVEAMIESSKEDAAAEAAAKEKAE
AEKEQASQTELDKEPIADEIEFDAFSAVDMRIARIISCEEVPKANKLLKFQLDIGGETRQVFSGIKSAYKPEELEGKLTV
MVANLKPRKMKFGMSEGMILAAGPGGSDLWILEPHEGAQPGMRVM

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=593, Percent_Identity=29.5109612141653, Blast_Score=273, Evalue=5e-73,
Organism=Homo sapiens, GI24308436, Length=528, Percent_Identity=26.7045454545455, Blast_Score=180, Evalue=4e-45,
Organism=Homo sapiens, GI4507947, Length=148, Percent_Identity=32.4324324324324, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788432, Length=687, Percent_Identity=70.0145560407569, Blast_Score=1042, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=588, Percent_Identity=29.9319727891156, Blast_Score=271, Evalue=7e-73,
Organism=Caenorhabditis elegans, GI212645096, Length=518, Percent_Identity=25.2895752895753, Blast_Score=157, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71992781, Length=413, Percent_Identity=24.6973365617433, Blast_Score=114, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321704, Length=555, Percent_Identity=29.3693693693694, Blast_Score=263, Evalue=8e-71,
Organism=Saccharomyces cerevisiae, GI6321610, Length=551, Percent_Identity=22.6860254083485, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24655311, Length=591, Percent_Identity=29.6108291032149, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI21358483, Length=516, Percent_Identity=24.8062015503876, Blast_Score=145, Evalue=1e-34,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 77854; Mature: 77723

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATDPRQLLVTCALPYANGSIHLGHMLEHIQADIWVRYQRLRGNTVNFICADDAHGTPIM
CCCCHHEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEE
LKAQQMGITPEEMIAAVSEEHQKDFAGFDISFDNYHSTHSEENRELASHIYLELKKNGFI
EEHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHEEECCCCCC
SSRTISQLFDPEKEMFLPDRFVKGTCPKCKSEDQYGDNCDNCGETYSPTELINPKSAVSG
CHHHHHHHHCCCHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHCCC
ATPVMKDSEHFFFDLPQFESMLKEWTRSGSLQNETANKMQEWFESGLQQWDISRDAPYFG
CCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FEIPGEKDKFFYVWLDAPVGYMASFKNLCDKRDDLNFDEYWKKDSTTELYHFIGKDIVYF
EECCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHH
HSLFWPAMLEGAGFRKPNNVFVHGYVTVNGAKMSKSKGTFIKASTYLNHLDPECLRYYYA
HHHHHHHHHCCCCCCCCCCEEEEEEEEEECCEECCCCCCEEEEHHHHHCCCHHHHHHHHH
AKLNSRIDDLDLNLEDFTQRVNADVVNKIVNLASRNAGFITKRFEGKLSAEFAEPELYNE
HHHCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHH
FVAAAERIGQLYETREFSRAIREITALADKANQYIDEKAPWVLAKEEGKEKELQEVSSVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHH
INLFRVLMAYLKPVMPELAARTEAFLNEELTWEAIATPLTDHEITKFKALFSRIDPKKVE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHCCHHHHH
AMIESSKEDAAAEAAAKEKAEAEKEQASQTELDKEPIADEIEFDAFSAVDMRIARIISCE
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EVPKANKLLKFQLDIGGETRQVFSGIKSAYKPEELEGKLTVMVANLKPRKMKFGMSEGMI
HCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHCCCCCCEE
LAAGPGGSDLWILEPHEGAQPGMRVM
EEECCCCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
ATDPRQLLVTCALPYANGSIHLGHMLEHIQADIWVRYQRLRGNTVNFICADDAHGTPIM
CCCHHEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEE
LKAQQMGITPEEMIAAVSEEHQKDFAGFDISFDNYHSTHSEENRELASHIYLELKKNGFI
EEHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHEEECCCCCC
SSRTISQLFDPEKEMFLPDRFVKGTCPKCKSEDQYGDNCDNCGETYSPTELINPKSAVSG
CHHHHHHHHCCCHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHCCC
ATPVMKDSEHFFFDLPQFESMLKEWTRSGSLQNETANKMQEWFESGLQQWDISRDAPYFG
CCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FEIPGEKDKFFYVWLDAPVGYMASFKNLCDKRDDLNFDEYWKKDSTTELYHFIGKDIVYF
EECCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHH
HSLFWPAMLEGAGFRKPNNVFVHGYVTVNGAKMSKSKGTFIKASTYLNHLDPECLRYYYA
HHHHHHHHHCCCCCCCCCCEEEEEEEEEECCEECCCCCCEEEEHHHHHCCCHHHHHHHHH
AKLNSRIDDLDLNLEDFTQRVNADVVNKIVNLASRNAGFITKRFEGKLSAEFAEPELYNE
HHHCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHH
FVAAAERIGQLYETREFSRAIREITALADKANQYIDEKAPWVLAKEEGKEKELQEVSSVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHH
INLFRVLMAYLKPVMPELAARTEAFLNEELTWEAIATPLTDHEITKFKALFSRIDPKKVE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHCCHHHHH
AMIESSKEDAAAEAAAKEKAEAEKEQASQTELDKEPIADEIEFDAFSAVDMRIARIISCE
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EVPKANKLLKFQLDIGGETRQVFSGIKSAYKPEELEGKLTVMVANLKPRKMKFGMSEGMI
HCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHCCCCCCEE
LAAGPGGSDLWILEPHEGAQPGMRVM
EEECCCCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA