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The map label for this gene is ackA1 [H]

Identifier: 218708992

GI number: 218708992

Start: 1033918

End: 1035114

Strand: Reverse

Name: ackA1 [H]

Synonym: VS_0998

Alternate gene names: 218708992

Gene position: 1035114-1033918 (Counterclockwise)

Preceding gene: 218708996

Following gene: 218708991

Centisome position: 31.37

GC content: 46.7

Gene sequence:

>1197_bases
ATGTCTAAGCTAGTTTTAGTTTTAAACTGTGGTAGTTCTTCTCTTAAATTCGCTGTTGTTGATGCAGAATCAGGTGCAGA
GCACCTAACTGGTCTTGCTGAGTGTCTAGGTCTTCCAGAAGCTCGTATGAAATGGAAACTTGATGGCAAGCATGAAGCAC
AACTAGGCGCGGGCGCAGCTCACGTAGAAGCACTATCTTTCATGGTAGAAACTATTCTTGCTTCTAAGCCTGAGCTTAAA
GCTAACCTTGGCGCTATCGGTCACCGTATCGTACACGGCGGCGAGCAGTTCACTTCTTCTGCTCTTATCACTGATGAAGT
TCTTAAGGGTATTCAAGACGCTGCGACTTTTGCACCTCTTCACAACCCTGCTCACCTTATCGGTATCGAAGCGGCTCAAA
AGAACTTCCCTGGTCTACAAAACGTTGCTGTATTTGACACTGCGTTCCACCAAACAATGCCTTCTGAGTCTTACCTATAC
GCTCTACCGTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCATGCACGGTACTTCTCACCTATTCATCACTCG
TGAAGTTGCAGGCCTACTAAACAAGCCAGTTGAAGAAGTTAACATCATCAACTGTCACCTAGGTAACGGCGCTTCTGTAT
GTGCAATCAAGAACGGTCAATCTGTAGATACTTCTATGGGTCTTACTCCTCTTGAAGGTCTTGTAATGGGTACACGTTGT
GGTGACCTAGATCCTGCAATCATCTTCCACCTACACGACGCTCTTGGTTACTCTGTTGAAGAAATCAACAACATGCTAAC
TAAAGAATCTGGTCTTGCTGGTCTAACTGAAGTGACTTCTGACTGTCGTTTCGTTGAAGACAACTACGGCGAGAAAGAAG
AAGCAACTCGTGCAATGGACGTGTTCTGTCACCGTCTAGCTAAGTACGTAGCTGGTTACACTGCAACTCTAGAAGGTCGT
CTAGACGCAATCACTTTCACTGGCGGCATCGGTGAGAACTCTGGCCCGATCCGTGAAATGGTTCTTAACCGCCTAGGCAT
CTTCGGCATCGAAGTTGACGGTGAAGCTAACCTTAAAGCTCGTTTCGGCGGCGAAGGTACTATCACTACAGCTAACAGCC
GTATCCCTGCAATGGTTATCTCGACTAACGAAGAGCTAGTAATTGCTGAAGACACTGCGAAGCTAGCAGGTCTTTAA

Upstream 100 bases:

>100_bases
TGACCAAGGTTAGTACGCAAGCCCATGTGCTTCGACTATTCTTGAGATTAATTTTCATCCTTAACTGATTTCGATCAGAA
AAATAAACAGGTAGTCATTA

Downstream 100 bases:

>100_bases
TTGATTTTCCTGACTAGCTTCACTCGAAGCTAGTCAGTTTTTCATATCAAGAAAATGGGGCTCAACTTCTATTCCTACCC
CAATATGTTTATCGGTGGGA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase 1 [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSKLVLVLNCGSSSLKFAVVDAESGAEHLTGLAECLGLPEARMKWKLDGKHEAQLGAGAAHVEALSFMVETILASKPELK
ANLGAIGHRIVHGGEQFTSSALITDEVLKGIQDAATFAPLHNPAHLIGIEAAQKNFPGLQNVAVFDTAFHQTMPSESYLY
ALPYNLYKEHGIRRYGMHGTSHLFITREVAGLLNKPVEEVNIINCHLGNGASVCAIKNGQSVDTSMGLTPLEGLVMGTRC
GDLDPAIIFHLHDALGYSVEEINNMLTKESGLAGLTEVTSDCRFVEDNYGEKEEATRAMDVFCHRLAKYVAGYTATLEGR
LDAITFTGGIGENSGPIREMVLNRLGIFGIEVDGEANLKARFGGEGTITTANSRIPAMVISTNEELVIAEDTAKLAGL

Sequences:

>Translated_398_residues
MSKLVLVLNCGSSSLKFAVVDAESGAEHLTGLAECLGLPEARMKWKLDGKHEAQLGAGAAHVEALSFMVETILASKPELK
ANLGAIGHRIVHGGEQFTSSALITDEVLKGIQDAATFAPLHNPAHLIGIEAAQKNFPGLQNVAVFDTAFHQTMPSESYLY
ALPYNLYKEHGIRRYGMHGTSHLFITREVAGLLNKPVEEVNIINCHLGNGASVCAIKNGQSVDTSMGLTPLEGLVMGTRC
GDLDPAIIFHLHDALGYSVEEINNMLTKESGLAGLTEVTSDCRFVEDNYGEKEEATRAMDVFCHRLAKYVAGYTATLEGR
LDAITFTGGIGENSGPIREMVLNRLGIFGIEVDGEANLKARFGGEGTITTANSRIPAMVISTNEELVIAEDTAKLAGL
>Mature_397_residues
SKLVLVLNCGSSSLKFAVVDAESGAEHLTGLAECLGLPEARMKWKLDGKHEAQLGAGAAHVEALSFMVETILASKPELKA
NLGAIGHRIVHGGEQFTSSALITDEVLKGIQDAATFAPLHNPAHLIGIEAAQKNFPGLQNVAVFDTAFHQTMPSESYLYA
LPYNLYKEHGIRRYGMHGTSHLFITREVAGLLNKPVEEVNIINCHLGNGASVCAIKNGQSVDTSMGLTPLEGLVMGTRCG
DLDPAIIFHLHDALGYSVEEINNMLTKESGLAGLTEVTSDCRFVEDNYGEKEEATRAMDVFCHRLAKYVAGYTATLEGRL
DAITFTGGIGENSGPIREMVLNRLGIFGIEVDGEANLKARFGGEGTITTANSRIPAMVISTNEELVIAEDTAKLAGL

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=397, Percent_Identity=72.7959697732997, Blast_Score=603, Evalue=1e-174,
Organism=Escherichia coli, GI145693184, Length=395, Percent_Identity=45.8227848101266, Blast_Score=345, Evalue=3e-96,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 42579; Mature: 42448

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLVLVLNCGSSSLKFAVVDAESGAEHLTGLAECLGLPEARMKWKLDGKHEAQLGAGAA
CCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHEEEEECCCCCHHHCCCHH
HVEALSFMVETILASKPELKANLGAIGHRIVHGGEQFTSSALITDEVLKGIQDAATFAPL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
HNPAHLIGIEAAQKNFPGLQNVAVFDTAFHQTMPSESYLYALPYNLYKEHGIRRYGMHGT
CCCCEEEEEEHHHCCCCCCCCEEEEHHHHHHCCCCCCEEEECCHHHHHHCCCEEECCCCC
SHLFITREVAGLLNKPVEEVNIINCHLGNGASVCAIKNGQSVDTSMGLTPLEGLVMGTRC
CEEEEHHHHHHHHHCCHHHEEEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHEEEECCC
GDLDPAIIFHLHDALGYSVEEINNMLTKESGLAGLTEVTSDCRFVEDNYGEKEEATRAMD
CCCCCCCEEEEHHHHCCCHHHHHHHHHHCCCCCHHHHHHHCCEEECCCCCCHHHHHHHHH
VFCHRLAKYVAGYTATLEGRLDAITFTGGIGENSGPIREMVLNRLGIFGIEVDGEANLKA
HHHHHHHHHHCCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHCCEEEEEECCCCCEEE
RFGGEGTITTANSRIPAMVISTNEELVIAEDTAKLAGL
EECCCEEEEECCCCCCEEEEECCCCEEEEECCHHHCCC
>Mature Secondary Structure 
SKLVLVLNCGSSSLKFAVVDAESGAEHLTGLAECLGLPEARMKWKLDGKHEAQLGAGAA
CCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHEEEEECCCCCHHHCCCHH
HVEALSFMVETILASKPELKANLGAIGHRIVHGGEQFTSSALITDEVLKGIQDAATFAPL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
HNPAHLIGIEAAQKNFPGLQNVAVFDTAFHQTMPSESYLYALPYNLYKEHGIRRYGMHGT
CCCCEEEEEEHHHCCCCCCCCEEEEHHHHHHCCCCCCEEEECCHHHHHHCCCEEECCCCC
SHLFITREVAGLLNKPVEEVNIINCHLGNGASVCAIKNGQSVDTSMGLTPLEGLVMGTRC
CEEEEHHHHHHHHHCCHHHEEEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHEEEECCC
GDLDPAIIFHLHDALGYSVEEINNMLTKESGLAGLTEVTSDCRFVEDNYGEKEEATRAMD
CCCCCCCEEEEHHHHCCCHHHHHHHHHHCCCCCHHHHHHHCCEEECCCCCCHHHHHHHHH
VFCHRLAKYVAGYTATLEGRLDAITFTGGIGENSGPIREMVLNRLGIFGIEVDGEANLKA
HHHHHHHHHHCCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHCCEEEEEECCCCCEEE
RFGGEGTITTANSRIPAMVISTNEELVIAEDTAKLAGL
EECCCEEEEECCCCCCEEEEECCCCEEEEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA