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The map label for this gene is dusC [H]

Identifier: 218708996

GI number: 218708996

Start: 1038947

End: 1039903

Strand: Reverse

Name: dusC [H]

Synonym: VS_1002

Alternate gene names: 218708996

Gene position: 1039903-1038947 (Counterclockwise)

Preceding gene: 218708997

Following gene: 218708992

Centisome position: 31.52

GC content: 44.31

Gene sequence:

>957_bases
ATGCGAGTAATACTTGGCCCTATGGAGGGCGTTTTAGACCATCTAATGCGTGAAATTCTCACAGAGATCAATGATTACGA
TCTCTGTGTGACGGAATTTGTGCGCGTAGTTGATCAACTTCTTCCTCCGCATGTTTTTCACCGTATTTGCCCTGAATTGC
ATCAAGGCTCTCAAACCATGGCTGGTGTTCCAATCCATCTCCAGCTTCTAGGACAACACCCAAATTGGATGGCCGAGAAT
GCTTTTCAAGCTGCTAGTTTAGGCGCTAAAGGCATTGATCTTAATTTTGGTTGCCCTGCAAAAGCGGTAAATAAAAGTAA
TGGTGGTGCGTCACTGCTAAAAGAACCTGAACTCATCTATCAAGTGGTGAAGTCTTGTCGAGATGCGGTGCCAGCACACA
TTCCCGTATCTGCGAAAATTCGATTGGGTTGGGAACGCCCGGAAGAGTGCTTTGAAATTGTCGACGCTATCGAACAAGCA
AAGGCAGACGAACTCACTGTGCATGCAAGAACCAAAGTTGGCGGCTATAAAGCCAGCGAAATTAAATGGGATTACATCAA
TCAAATAAGAGAAAAAACGTCTCTACCTTTGATTGCGAATGGGGAAATCTGGAACTATCAAGATGGTCAAGATTGCATAG
AAGCCACTGGCGTCGATTCATTGATGGTTTGCCGTGGCGCTTTCAACATTCCAAATCTTGGCAACGTCGTTAAGCACAAC
CACCCAAAAATGCCGTGGAATAAAGTCATCGAACTTTTGCTACGCTATTCGGAGTTTCAAATAAAAGGGGACAAAGGCAT
GTACTACCCCAATAGAGTGAAGCAGTGGTTTGTTTATCTAAGTAAAGGCTACCCTGAAGCGAACGAACTATTTAAAGAAA
TTCGTACTTTCAAGAAAGCGCCACCTATTGTGGAGCGCCTTCAGCGTTATCAAAAGGAACATCGCCAAACTGTGTGA

Upstream 100 bases:

>100_bases
ACTGTTACGTCAGATTCACCTAGCTCATAGTGACAAGCTTTTAACTTGCCCGTACAATCACGCGTCTTAATTTTCGATCC
ACTCAGTTTCGGTAAAGCAA

Downstream 100 bases:

>100_bases
GTTAGTTCCCCTTATTCTCTAGCATAGAAGTTCAATAGCGTGGTTAAAATACGATGCGGTCTTTACCTGTCGTCTTAGCC
ACGTACAACTTTTCATCCGC

Product: tRNA-dihydrouridine synthase C

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MRVILGPMEGVLDHLMREILTEINDYDLCVTEFVRVVDQLLPPHVFHRICPELHQGSQTMAGVPIHLQLLGQHPNWMAEN
AFQAASLGAKGIDLNFGCPAKAVNKSNGGASLLKEPELIYQVVKSCRDAVPAHIPVSAKIRLGWERPEECFEIVDAIEQA
KADELTVHARTKVGGYKASEIKWDYINQIREKTSLPLIANGEIWNYQDGQDCIEATGVDSLMVCRGAFNIPNLGNVVKHN
HPKMPWNKVIELLLRYSEFQIKGDKGMYYPNRVKQWFVYLSKGYPEANELFKEIRTFKKAPPIVERLQRYQKEHRQTV

Sequences:

>Translated_318_residues
MRVILGPMEGVLDHLMREILTEINDYDLCVTEFVRVVDQLLPPHVFHRICPELHQGSQTMAGVPIHLQLLGQHPNWMAEN
AFQAASLGAKGIDLNFGCPAKAVNKSNGGASLLKEPELIYQVVKSCRDAVPAHIPVSAKIRLGWERPEECFEIVDAIEQA
KADELTVHARTKVGGYKASEIKWDYINQIREKTSLPLIANGEIWNYQDGQDCIEATGVDSLMVCRGAFNIPNLGNVVKHN
HPKMPWNKVIELLLRYSEFQIKGDKGMYYPNRVKQWFVYLSKGYPEANELFKEIRTFKKAPPIVERLQRYQKEHRQTV
>Mature_318_residues
MRVILGPMEGVLDHLMREILTEINDYDLCVTEFVRVVDQLLPPHVFHRICPELHQGSQTMAGVPIHLQLLGQHPNWMAEN
AFQAASLGAKGIDLNFGCPAKAVNKSNGGASLLKEPELIYQVVKSCRDAVPAHIPVSAKIRLGWERPEECFEIVDAIEQA
KADELTVHARTKVGGYKASEIKWDYINQIREKTSLPLIANGEIWNYQDGQDCIEATGVDSLMVCRGAFNIPNLGNVVKHN
HPKMPWNKVIELLLRYSEFQIKGDKGMYYPNRVKQWFVYLSKGYPEANELFKEIRTFKKAPPIVERLQRYQKEHRQTV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=165, Percent_Identity=32.7272727272727, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI40807366, Length=239, Percent_Identity=26.7782426778243, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI239788483, Length=228, Percent_Identity=24.1228070175439, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI239788462, Length=228, Percent_Identity=24.1228070175439, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI8923374, Length=187, Percent_Identity=30.4812834224599, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788462, Length=308, Percent_Identity=57.7922077922078, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1789660, Length=260, Percent_Identity=31.5384615384615, Blast_Score=115, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI25144369, Length=214, Percent_Identity=29.4392523364486, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=204, Percent_Identity=25.4901960784314, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17507177, Length=144, Percent_Identity=32.6388888888889, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17510279, Length=168, Percent_Identity=29.7619047619048, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=203, Percent_Identity=27.5862068965517, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI19921524, Length=147, Percent_Identity=34.6938775510204, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24580595, Length=171, Percent_Identity=29.2397660818713, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920448, Length=171, Percent_Identity=29.2397660818713, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24585320, Length=258, Percent_Identity=27.906976744186, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36212; Mature: 36212

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVILGPMEGVLDHLMREILTEINDYDLCVTEFVRVVDQLLPPHVFHRICPELHQGSQTM
CCEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
AGVPIHLQLLGQHPNWMAENAFQAASLGAKGIDLNFGCPAKAVNKSNGGASLLKEPELIY
CCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCHHHHCCHHHHH
QVVKSCRDAVPAHIPVSAKIRLGWERPEECFEIVDAIEQAKADELTVHARTKVGGYKASE
HHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCEECCC
IKWDYINQIREKTSLPLIANGEIWNYQDGQDCIEATGVDSLMVCRGAFNIPNLGNVVKHN
CCHHHHHHHHHHHCCCEEECCCEECCCCCHHHHHHCCCCHHHHHHCCCCCCCCCHHHHCC
HPKMPWNKVIELLLRYSEFQIKGDKGMYYPNRVKQWFVYLSKGYPEANELFKEIRTFKKA
CCCCCHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
PPIVERLQRYQKEHRQTV
CHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRVILGPMEGVLDHLMREILTEINDYDLCVTEFVRVVDQLLPPHVFHRICPELHQGSQTM
CCEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
AGVPIHLQLLGQHPNWMAENAFQAASLGAKGIDLNFGCPAKAVNKSNGGASLLKEPELIY
CCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCHHHHCCHHHHH
QVVKSCRDAVPAHIPVSAKIRLGWERPEECFEIVDAIEQAKADELTVHARTKVGGYKASE
HHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCEECCC
IKWDYINQIREKTSLPLIANGEIWNYQDGQDCIEATGVDSLMVCRGAFNIPNLGNVVKHN
CCHHHHHHHHHHHCCCEEECCCEECCCCCHHHHHHCCCCHHHHHHCCCCCCCCCHHHHCC
HPKMPWNKVIELLLRYSEFQIKGDKGMYYPNRVKQWFVYLSKGYPEANELFKEIRTFKKA
CCCCCHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
PPIVERLQRYQKEHRQTV
CHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]