Click here to switch to the map view.

The map label for this gene is mleN [H]

Identifier: 218708950

GI number: 218708950

Start: 982196

End: 983632

Strand: Reverse

Name: mleN [H]

Synonym: VS_0954

Alternate gene names: 218708950

Gene position: 983632-982196 (Counterclockwise)

Preceding gene: 218708952

Following gene: 218708946

Centisome position: 29.81

GC content: 46.14

Gene sequence:

>1437_bases
ATGAAGCAGAGTAAAACTCGCCTACCGAACCTATTGCAGGTATTCATCGCGTTAGGATTATTTCTATCCCTTGCTTTTTC
CTTTACTGCAAAGCTTGACCTTCCAATTCAACTTGCCTTGTATATTGGCTGGTTCATTATCATGGTTCTCGGTATTCGCC
TTGGCCACGAATACAAAGACTTAGAAAAAGCGGCACTCAAAGGAATATCCAATGGCTTAGGCGCGGTTTTAATACTTTTA
GCCGTTGGCGCTCTTGTTGGTACTTGGATCTCAGGCGGGATCGTACCTACTATCATTTACTATGGTCTGAAAGCTATCCA
CCCTTCTATCTTCCTTTTAGCGACCATGATTATCTGTTCTCTAACCGCATTGGCAACTGGTACTTCTTGGGGCGCTGCGG
GTACAGCAGGTATCGCGATGATGGGCATTGGCCAAGGTCTAGGTGTTCCAGCACCGATTACTGCAGGTGCAGTGCTGTCA
GGTTGTTACTTCGGCGACAAGATGTCTCCACTTTCTGATTCAGTGATATTGGCTTCTTCAATGTCTGGTGTTGAAGTGGT
TGAGCACATCAAGGGCATGCTGCCAGTTGCGTTAATCAGCTATGTGATTACTGGCATCATGTTTACTGCGTTTGGTTTTC
ACTACGCAGGTAACGTTGACATGAGCCAAGTAGATTCTGTCATTAAAGCAATGGAAGTTCAGTTCTACATTACGCCTTAC
TCATTTGTCCCAGTACTGATCGTGCTTGGTCTGTTGGCTTTCCGCATGCCTTCATTCCCGGTAATCAGCTTTGGTTCTCT
GCTAGGTATTATCTGGGCAGTCATGATCCAAGATATCGATTTCCTAACGGCATTCAATACTGCCTGGGCACCATTCTCTA
TCTCCTCTGGCGTAGAGTTTATTGACTCGATTCTTAACCGTGGCGGCATGTCTTCAATGCTTGGTTCGGTAGCGGTGATT
GTGTTTGGTTTAGGTTTTGGTGGCTTACTGGATAAAGTGGGCGTATTAGAGACGATTGCTAAGGTGTTCGAGCGCCGAGT
AAATAGCGCTGGTTCTCTAGCAACAAGCACGATCGGCACGGCCTTCATGGGTAACGTATTCGGTTCTGCAATGTACGTAT
CGTTAATCCTAACGCCAAAAATCTGTGCGAAAAACTACGACCGTTTAGGTTACCAACGTAAAAATCTATCTCGTAACGCT
GAGTTCGGTGGCACGTTAACTTCAGGCATGGTTCCTTGGAGTGACAACGGCATCTACATGGCGAGTATTCTTGGTGTCGC
AACGCTCTCTTACGCTCCGTTCATGTGGTTGAGCTTCATCTGTATCCTCGTGACTATCGTAACGTCTTACATGGGTTGGT
TCGTTGATAAATGTGAACCAACAGCACCAGCGCTTGAAACTGAAGAAGCCACTGAACTGAGCAAGCAACAAGCTTAA

Upstream 100 bases:

>100_bases
GCGTATTTTTTCGATATAATAAACTAATTACTGTAGTGTCCGTTTTGCACCACACACCATAACGAAGCACTATAAATGCT
CGAAATCACAAGAGGCTTTT

Downstream 100 bases:

>100_bases
GCTAATGTTTTGATACTTTAATGCTTTAGTGCGTAAGCACAAAAATGTTGATGATATAAATGCCAAAAGAGCGCTCTAGA
GCGCTCTTTTTTGATCTACT

Product: putative Na+/H+ antiporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MKQSKTRLPNLLQVFIALGLFLSLAFSFTAKLDLPIQLALYIGWFIIMVLGIRLGHEYKDLEKAALKGISNGLGAVLILL
AVGALVGTWISGGIVPTIIYYGLKAIHPSIFLLATMIICSLTALATGTSWGAAGTAGIAMMGIGQGLGVPAPITAGAVLS
GCYFGDKMSPLSDSVILASSMSGVEVVEHIKGMLPVALISYVITGIMFTAFGFHYAGNVDMSQVDSVIKAMEVQFYITPY
SFVPVLIVLGLLAFRMPSFPVISFGSLLGIIWAVMIQDIDFLTAFNTAWAPFSISSGVEFIDSILNRGGMSSMLGSVAVI
VFGLGFGGLLDKVGVLETIAKVFERRVNSAGSLATSTIGTAFMGNVFGSAMYVSLILTPKICAKNYDRLGYQRKNLSRNA
EFGGTLTSGMVPWSDNGIYMASILGVATLSYAPFMWLSFICILVTIVTSYMGWFVDKCEPTAPALETEEATELSKQQA

Sequences:

>Translated_478_residues
MKQSKTRLPNLLQVFIALGLFLSLAFSFTAKLDLPIQLALYIGWFIIMVLGIRLGHEYKDLEKAALKGISNGLGAVLILL
AVGALVGTWISGGIVPTIIYYGLKAIHPSIFLLATMIICSLTALATGTSWGAAGTAGIAMMGIGQGLGVPAPITAGAVLS
GCYFGDKMSPLSDSVILASSMSGVEVVEHIKGMLPVALISYVITGIMFTAFGFHYAGNVDMSQVDSVIKAMEVQFYITPY
SFVPVLIVLGLLAFRMPSFPVISFGSLLGIIWAVMIQDIDFLTAFNTAWAPFSISSGVEFIDSILNRGGMSSMLGSVAVI
VFGLGFGGLLDKVGVLETIAKVFERRVNSAGSLATSTIGTAFMGNVFGSAMYVSLILTPKICAKNYDRLGYQRKNLSRNA
EFGGTLTSGMVPWSDNGIYMASILGVATLSYAPFMWLSFICILVTIVTSYMGWFVDKCEPTAPALETEEATELSKQQA
>Mature_478_residues
MKQSKTRLPNLLQVFIALGLFLSLAFSFTAKLDLPIQLALYIGWFIIMVLGIRLGHEYKDLEKAALKGISNGLGAVLILL
AVGALVGTWISGGIVPTIIYYGLKAIHPSIFLLATMIICSLTALATGTSWGAAGTAGIAMMGIGQGLGVPAPITAGAVLS
GCYFGDKMSPLSDSVILASSMSGVEVVEHIKGMLPVALISYVITGIMFTAFGFHYAGNVDMSQVDSVIKAMEVQFYITPY
SFVPVLIVLGLLAFRMPSFPVISFGSLLGIIWAVMIQDIDFLTAFNTAWAPFSISSGVEFIDSILNRGGMSSMLGSVAVI
VFGLGFGGLLDKVGVLETIAKVFERRVNSAGSLATSTIGTAFMGNVFGSAMYVSLILTPKICAKNYDRLGYQRKNLSRNA
EFGGTLTSGMVPWSDNGIYMASILGVATLSYAPFMWLSFICILVTIVTSYMGWFVDKCEPTAPALETEEATELSKQQA

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 50862; Mature: 50862

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSKTRLPNLLQVFIALGLFLSLAFSFTAKLDLPIQLALYIGWFIIMVLGIRLGHEYKD
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
LEKAALKGISNGLGAVLILLAVGALVGTWISGGIVPTIIYYGLKAIHPSIFLLATMIICS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTALATGTSWGAAGTAGIAMMGIGQGLGVPAPITAGAVLSGCYFGDKMSPLSDSVILASS
HHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEECCC
MSGVEVVEHIKGMLPVALISYVITGIMFTAFGFHYAGNVDMSQVDSVIKAMEVQFYITPY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEECCH
SFVPVLIVLGLLAFRMPSFPVISFGSLLGIIWAVMIQDIDFLTAFNTAWAPFSISSGVEF
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHH
IDSILNRGGMSSMLGSVAVIVFGLGFGGLLDKVGVLETIAKVFERRVNSAGSLATSTIGT
HHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AFMGNVFGSAMYVSLILTPKICAKNYDRLGYQRKNLSRNAEFGGTLTSGMVPWSDNGIYM
HHHHHHHHHHHHHHHHHCHHHHHCCHHHHCHHHHCCCCCCCCCCCHHCCCCCCCCCCCHH
ASILGVATLSYAPFMWLSFICILVTIVTSYMGWFVDKCEPTAPALETEEATELSKQQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MKQSKTRLPNLLQVFIALGLFLSLAFSFTAKLDLPIQLALYIGWFIIMVLGIRLGHEYKD
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
LEKAALKGISNGLGAVLILLAVGALVGTWISGGIVPTIIYYGLKAIHPSIFLLATMIICS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTALATGTSWGAAGTAGIAMMGIGQGLGVPAPITAGAVLSGCYFGDKMSPLSDSVILASS
HHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEECCC
MSGVEVVEHIKGMLPVALISYVITGIMFTAFGFHYAGNVDMSQVDSVIKAMEVQFYITPY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEECCH
SFVPVLIVLGLLAFRMPSFPVISFGSLLGIIWAVMIQDIDFLTAFNTAWAPFSISSGVEF
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHH
IDSILNRGGMSSMLGSVAVIVFGLGFGGLLDKVGVLETIAKVFERRVNSAGSLATSTIGT
HHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AFMGNVFGSAMYVSLILTPKICAKNYDRLGYQRKNLSRNAEFGGTLTSGMVPWSDNGIYM
HHHHHHHHHHHHHHHHHCHHHHHCCHHHHCHHHHCCCCCCCCCCCHHCCCCCCCCCCCHH
ASILGVATLSYAPFMWLSFICILVTIVTSYMGWFVDKCEPTAPALETEEATELSKQQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]