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The map label for this gene is topB [H]

Identifier: 218708938

GI number: 218708938

Start: 968110

End: 970101

Strand: Direct

Name: topB [H]

Synonym: VS_0940

Alternate gene names: 218708938

Gene position: 968110-970101 (Clockwise)

Preceding gene: 218708937

Following gene: 218708941

Centisome position: 29.34

GC content: 48.59

Gene sequence:

>1992_bases
ATGTCTCGTCTTATTATTGCTGAAAAACCAAGCCTCGGCCGTGCGATCGCCGCTGCATTGCCTAATCCACAGAAGAAAGA
CCAAGGGTTTATCAAATGTGGCAATGGGGATGTGGTGACTTGGTGTATTGGACACTTATTGGAGCAGGTTGAGCCAGACG
CTTATGACGACCGTTATAAGAAGTGGAACTTAGCCGATCTTCCTATCGTGCCGGAGCAATGGCAGCTAAGACCGCGTAAG
ACGTCAAGCAAACAGCTCACGCTGATCCGAAAGCTATTGAAGGACGCGACCCAAATTGTCCATGCGGGAGACCCGGATAG
AGAAGGGCAGTTGCTGGTTGATGAAGTGATTGATTATTGCAAGGTGTCTAAGGCTAAGAAAGAGTCGATGGACAGGCTGC
TGATCAGCGATTTGAACTTACCAGCGGTAAAGCGTGCGCTCTCTCAGATGCGGAGTAACCGCGATTTTATTCCACTTTCT
ATTTCTGCATTGGCGCGCTCTAGAGCCGATTGGCTGTATGGCATGAACATGACTCGCGCGTATACCTTGCTTGGTCAAAA
GGCGGGTTACCAAGGTGTGTTGTCGGTAGGGCGAGTGCAAACGCCTGTACTTGGTTTGGTCGTAAGGCGTGATGAAGAGA
TCGAAAACTTCATTCCGAAAGATTACTTCACTCTGCATGCGCTGATCCCTTACCAAAACAACGGCCAGAGCTTTGATATT
CGTGCGCGTTGGAAGCCAAGCGAAGCATGTAAACCTTGGCAAGATGAAGAAGGCCGCGTGCTCAATCGAAAGCTGGTTGA
GAATGTCGCTAACCGAATTGCTAATCAGCCTGCAACGGTGACGGAATCAGAGCAAAAGCAAAGCAAACAAGCGGCACCAC
TCCCTTATTCGCTGTCAGCTCTGCAGATTGATGCGTCTAAGCGTTTTGGTATGAGCGCTCAACAGGTACTCGACACTTGT
CAGTCATTGTATGAGAAACACAAACTCATCACTTACCCACGTTCTGATAGCCGCTATCTACCTAAAGATCATTATTCGCA
ACGAGAGTCAGTCGTGGATGCTATCGCCAATAATGCGAAAGAGCTGCAAAGTGGTGCGCAAGGCGCGGATCTTTCTCTTA
AATCCAAAGCATGGAACGACAGTAAGGTCGATGCTCACCACGCGATAATCCCAACGCCGAAGAAATCAGCGGTGAATGGC
CTATCTGCCAATGAGATGAAAATCTATCAGCAAATCGCCCGCCAATATCTGATGCAGTTCTACCCACCGGCTGTTTTTGC
GGATGCTAAGTTGGTTTTTGATATCGCTGGTGGTGTGTTCATCGCGAAAGGGCGTCAGCTTATCAACCCTGGTTGGAAGG
TGTTGATGGGAAAAACGGACACCGAAGAGAAAGGCGATGGCACAGATACGGTTCCTCCATTGGATAAAGGAACGGTGCTG
ACGTGTCGTGAAGGTATTATTGGCGATAAGAAAACCGAGCCACCAAAACACTTTACCGAAGCGACCTTGTTACAAGCTAT
GACCGGTATTGCGCGCTTTGTGGCAAACAAAGACCTTAAAGCTATTTTGAAAGAGACCGATGGCCTTGGAACAGAGGCAA
CTCGTGCGGGCATTCTAGATACTTTGTTCAAAAGACAGCTACTAACTCGACAAGGTAAAAGCATTCATAGCAGCCCTGCG
GGGAGAGGTTTGATTAATGCCTTGCCTGAGGACTCGACCTTTCCCGATATGACCGCTCACTGGGAGCATCAGTTACAAGG
TATGGCTGAACGAAACCAAGCGTATCAGCCCTTCATGCAAGAATTAGAAAGCAAGATCGACGGCTTGATGGGCAAGGTAA
AAACGGGTGAAGTTCCTGAATCCCTGCGCCATCTTCCTAAAGTCGAAAGACCTGCCTTTAAACGCCGCAAAGGTGGTGGT
GCGAAGTCTTATGCCAACAAAGGTACGTATGCCAAAAAAGGGACGTATGCTAAGAAAGGCACTAAAAGCTAG

Upstream 100 bases:

>100_bases
GAATTGTTGAATGTTAACTGTATAAACAACCAGTTTTCCTTGATTTCTAGGGGCCACATTAGATAATGTGGCCCTAATTG
TTTGTAGCATCTGGAAAAAC

Downstream 100 bases:

>100_bases
GAACTGAGAGCAAACCGCTGTTTATTGGCGATACTGGAATTAACGCCCAACCTTGCGTCCGCAGCGCTTAAACAGCTGTT
GCCAATAGTCGACATGGCGC

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 663; Mature: 662

Protein sequence:

>663_residues
MSRLIIAEKPSLGRAIAAALPNPQKKDQGFIKCGNGDVVTWCIGHLLEQVEPDAYDDRYKKWNLADLPIVPEQWQLRPRK
TSSKQLTLIRKLLKDATQIVHAGDPDREGQLLVDEVIDYCKVSKAKKESMDRLLISDLNLPAVKRALSQMRSNRDFIPLS
ISALARSRADWLYGMNMTRAYTLLGQKAGYQGVLSVGRVQTPVLGLVVRRDEEIENFIPKDYFTLHALIPYQNNGQSFDI
RARWKPSEACKPWQDEEGRVLNRKLVENVANRIANQPATVTESEQKQSKQAAPLPYSLSALQIDASKRFGMSAQQVLDTC
QSLYEKHKLITYPRSDSRYLPKDHYSQRESVVDAIANNAKELQSGAQGADLSLKSKAWNDSKVDAHHAIIPTPKKSAVNG
LSANEMKIYQQIARQYLMQFYPPAVFADAKLVFDIAGGVFIAKGRQLINPGWKVLMGKTDTEEKGDGTDTVPPLDKGTVL
TCREGIIGDKKTEPPKHFTEATLLQAMTGIARFVANKDLKAILKETDGLGTEATRAGILDTLFKRQLLTRQGKSIHSSPA
GRGLINALPEDSTFPDMTAHWEHQLQGMAERNQAYQPFMQELESKIDGLMGKVKTGEVPESLRHLPKVERPAFKRRKGGG
AKSYANKGTYAKKGTYAKKGTKS

Sequences:

>Translated_663_residues
MSRLIIAEKPSLGRAIAAALPNPQKKDQGFIKCGNGDVVTWCIGHLLEQVEPDAYDDRYKKWNLADLPIVPEQWQLRPRK
TSSKQLTLIRKLLKDATQIVHAGDPDREGQLLVDEVIDYCKVSKAKKESMDRLLISDLNLPAVKRALSQMRSNRDFIPLS
ISALARSRADWLYGMNMTRAYTLLGQKAGYQGVLSVGRVQTPVLGLVVRRDEEIENFIPKDYFTLHALIPYQNNGQSFDI
RARWKPSEACKPWQDEEGRVLNRKLVENVANRIANQPATVTESEQKQSKQAAPLPYSLSALQIDASKRFGMSAQQVLDTC
QSLYEKHKLITYPRSDSRYLPKDHYSQRESVVDAIANNAKELQSGAQGADLSLKSKAWNDSKVDAHHAIIPTPKKSAVNG
LSANEMKIYQQIARQYLMQFYPPAVFADAKLVFDIAGGVFIAKGRQLINPGWKVLMGKTDTEEKGDGTDTVPPLDKGTVL
TCREGIIGDKKTEPPKHFTEATLLQAMTGIARFVANKDLKAILKETDGLGTEATRAGILDTLFKRQLLTRQGKSIHSSPA
GRGLINALPEDSTFPDMTAHWEHQLQGMAERNQAYQPFMQELESKIDGLMGKVKTGEVPESLRHLPKVERPAFKRRKGGG
AKSYANKGTYAKKGTYAKKGTKS
>Mature_662_residues
SRLIIAEKPSLGRAIAAALPNPQKKDQGFIKCGNGDVVTWCIGHLLEQVEPDAYDDRYKKWNLADLPIVPEQWQLRPRKT
SSKQLTLIRKLLKDATQIVHAGDPDREGQLLVDEVIDYCKVSKAKKESMDRLLISDLNLPAVKRALSQMRSNRDFIPLSI
SALARSRADWLYGMNMTRAYTLLGQKAGYQGVLSVGRVQTPVLGLVVRRDEEIENFIPKDYFTLHALIPYQNNGQSFDIR
ARWKPSEACKPWQDEEGRVLNRKLVENVANRIANQPATVTESEQKQSKQAAPLPYSLSALQIDASKRFGMSAQQVLDTCQ
SLYEKHKLITYPRSDSRYLPKDHYSQRESVVDAIANNAKELQSGAQGADLSLKSKAWNDSKVDAHHAIIPTPKKSAVNGL
SANEMKIYQQIARQYLMQFYPPAVFADAKLVFDIAGGVFIAKGRQLINPGWKVLMGKTDTEEKGDGTDTVPPLDKGTVLT
CREGIIGDKKTEPPKHFTEATLLQAMTGIARFVANKDLKAILKETDGLGTEATRAGILDTLFKRQLLTRQGKSIHSSPAG
RGLINALPEDSTFPDMTAHWEHQLQGMAERNQAYQPFMQELESKIDGLMGKVKTGEVPESLRHLPKVERPAFKRRKGGGA
KSYANKGTYAKKGTYAKKGTKS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=626, Percent_Identity=22.5239616613419, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI10835218, Length=570, Percent_Identity=22.9824561403509, Blast_Score=96, Evalue=1e-19,
Organism=Escherichia coli, GI1788061, Length=616, Percent_Identity=59.4155844155844, Blast_Score=764, Evalue=0.0,
Organism=Escherichia coli, GI1787529, Length=566, Percent_Identity=23.6749116607774, Blast_Score=111, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32563869, Length=623, Percent_Identity=24.2375601926164, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17555378, Length=670, Percent_Identity=23.5820895522388, Blast_Score=99, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6323263, Length=393, Percent_Identity=23.9185750636132, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24585251, Length=587, Percent_Identity=23.3390119250426, Blast_Score=92, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 73891; Mature: 73759

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLIIAEKPSLGRAIAAALPNPQKKDQGFIKCGNGDVVTWCIGHLLEQVEPDAYDDRYK
CCCEEEECCCCHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCHHC
KWNLADLPIVPEQWQLRPRKTSSKQLTLIRKLLKDATQIVHAGDPDREGQLLVDEVIDYC
CCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
KVSKAKKESMDRLLISDLNLPAVKRALSQMRSNRDFIPLSISALARSRADWLYGMNMTRA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCHHHH
YTLLGQKAGYQGVLSVGRVQTPVLGLVVRRDEEIENFIPKDYFTLHALIPYQNNGQSFDI
HHHHHHCCCCHHHHHHCCHHCCHHHHHHHCCHHHHHCCCCCEEEEEEEEEECCCCCEEEE
RARWKPSEACKPWQDEEGRVLNRKLVENVANRIANQPATVTESEQKQSKQAAPLPYSLSA
EECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCE
LQIDASKRFGMSAQQVLDTCQSLYEKHKLITYPRSDSRYLPKDHYSQRESVVDAIANNAK
EEECCHHHCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCHH
ELQSGAQGADLSLKSKAWNDSKVDAHHAIIPTPKKSAVNGLSANEMKIYQQIARQYLMQF
HHHCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
YPPAVFADAKLVFDIAGGVFIAKGRQLINPGWKVLMGKTDTEEKGDGTDTVPPLDKGTVL
CCCHHHHCCEEEEECCCCEEEECCCHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEE
TCREGIIGDKKTEPPKHFTEATLLQAMTGIARFVANKDLKAILKETDGLGTEATRAGILD
EECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHH
TLFKRQLLTRQGKSIHSSPAGRGLINALPEDSTFPDMTAHWEHQLQGMAERNQAYQPFMQ
HHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ELESKIDGLMGKVKTGEVPESLRHLPKVERPAFKRRKGGGAKSYANKGTYAKKGTYAKKG
HHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCHHHHHCCCCCCHHCCCCCCCCCCCCCCCC
TKS
CCC
>Mature Secondary Structure 
SRLIIAEKPSLGRAIAAALPNPQKKDQGFIKCGNGDVVTWCIGHLLEQVEPDAYDDRYK
CCEEEECCCCHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCHHC
KWNLADLPIVPEQWQLRPRKTSSKQLTLIRKLLKDATQIVHAGDPDREGQLLVDEVIDYC
CCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
KVSKAKKESMDRLLISDLNLPAVKRALSQMRSNRDFIPLSISALARSRADWLYGMNMTRA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCHHHH
YTLLGQKAGYQGVLSVGRVQTPVLGLVVRRDEEIENFIPKDYFTLHALIPYQNNGQSFDI
HHHHHHCCCCHHHHHHCCHHCCHHHHHHHCCHHHHHCCCCCEEEEEEEEEECCCCCEEEE
RARWKPSEACKPWQDEEGRVLNRKLVENVANRIANQPATVTESEQKQSKQAAPLPYSLSA
EECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCE
LQIDASKRFGMSAQQVLDTCQSLYEKHKLITYPRSDSRYLPKDHYSQRESVVDAIANNAK
EEECCHHHCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCHH
ELQSGAQGADLSLKSKAWNDSKVDAHHAIIPTPKKSAVNGLSANEMKIYQQIARQYLMQF
HHHCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
YPPAVFADAKLVFDIAGGVFIAKGRQLINPGWKVLMGKTDTEEKGDGTDTVPPLDKGTVL
CCCHHHHCCEEEEECCCCEEEECCCHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEE
TCREGIIGDKKTEPPKHFTEATLLQAMTGIARFVANKDLKAILKETDGLGTEATRAGILD
EECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHH
TLFKRQLLTRQGKSIHSSPAGRGLINALPEDSTFPDMTAHWEHQLQGMAERNQAYQPFMQ
HHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ELESKIDGLMGKVKTGEVPESLRHLPKVERPAFKRRKGGGAKSYANKGTYAKKGTYAKKG
HHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCHHHHHCCCCCCHHCCCCCCCCCCCCCCCC
TKS
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]