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The map label for this gene is fadI [H]

Identifier: 218708864

GI number: 218708864

Start: 884502

End: 885809

Strand: Direct

Name: fadI [H]

Synonym: VS_0864

Alternate gene names: 218708864

Gene position: 884502-885809 (Clockwise)

Preceding gene: 218708859

Following gene: 218708865

Centisome position: 26.81

GC content: 48.93

Gene sequence:

>1308_bases
ATGGGCAAACAGGAAGTCAAAACGCGTTCTGGAGAACGTGTTGCCGTTGTCGCTGGACTACGAACCCCATTCGCTCGTCA
GAGCACAGAATTTAGCCAAGTGCCTGCGGTTGACCTAGGCAAAATGGTGGTGAGCGAAATGCTTGCAAGAACTGATGTCG
ATCCTGCGCTTATCGAACAAGTCGTGTTCGGCCAAGTAGTGCAAATGCCAGAAGCACCGAACATTGCACGTGAAATCGTG
CTGGGTACAGGTATGGACATTAATACTGATGCCTACAGTGTGACGCGAGCATGTGCGACTAGCTTCCAAGCTGCAGTCAA
CGTGACCGAGAGCATTATGGCTGGCACGATTGATGTTGGTATTGCGGGCGGCGCGGATTCTTCTTCTGTATTGCCAATCG
GTGTTTCGAAAAAGTTAGCGGCGAACTTATTAGCACTGAGTAAAACGAAAACCATAGGTCAAAAACTGAAGATTCTTAAA
ACATTTTCAGTCAAAGATTTGATGCCAGTACCACCAGCAGTCGCAGAATACTCGACAGGTTTGTCTATGGGACAAACTGC
TGAGCAGATGGCTAAGACACATGGTATTACTCGCGAAGCTCAAGATGCACTTGCTCACCGTTCTCACTCTTTGGCTTCTC
AAGCATGGAAAGAAGGCAAGATTAAAGACGAAGTGATGACGGCATTCCCCGCGCCTTATAAAAAGTACCTAGCAGAAGAC
AACAACATTCGCCACGACTCGACGGTTGAAGGTTACGCTAAATTGCGCCCTGCATTCGACAGACAATACGGCAGTGTAAC
GGCAGCCAATGCCACGCCTCTTACCGATGGCGGTGCCGCAGTGATGTTGATGCGCGAAGGAAAAGCGAAAGAGCTAGGCT
TAGAAGTGCTTGGTTACATTCGTGGTTACGCGTTCTCAGCGATTGGTGTTGAAACGGATATGCTGATGGGGCCGACGTAC
GCGACCTCTCAAGTATTGAAGAACACAGGACTAGAATTATCAGATCTAACACTTATCGAGATGCACGAAGCGTTTGCAGC
TCAAGTTCTGGCTAACGTCAAAATGTTCGCAAGCGATGAATTTGCTCAGAAGAATCTTGGCCGTGACAAAGCGATCGGTG
AGATTGATATGGAGAAGTTCAACGTGTTGGGTAGCTCAATTGCTTACGGACACCCGTTTGCAGCGACTGGCGCGCGTATG
ATGACTCAGACACTGCGTGAACTTAAACGTCGTGGTGGTGGCCTAGCACTGAATACTGCTTGTGCGGCTGGTGGCTTAGG
TGCAGCAATGATCTTGGAGGTGGAATAA

Upstream 100 bases:

>100_bases
GTGGGATACTCGTATCACGTTTATTTAAACGCCTGTTTGATTTAATTAACAACTTGTTTACAATACTTCAGGTCAGACCT
CTTAACTTTAAGGAGAAACC

Downstream 100 bases:

>100_bases
TGTCTATGACTCTTGATGTGAAAAAAGAAAAGAAAGCAGTCAAAAAAACGGATTCAAAGCCAGTGACTGTATCTAAAAGT
GATAAGACGGTTTCAAACGA

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MGKQEVKTRSGERVAVVAGLRTPFARQSTEFSQVPAVDLGKMVVSEMLARTDVDPALIEQVVFGQVVQMPEAPNIAREIV
LGTGMDINTDAYSVTRACATSFQAAVNVTESIMAGTIDVGIAGGADSSSVLPIGVSKKLAANLLALSKTKTIGQKLKILK
TFSVKDLMPVPPAVAEYSTGLSMGQTAEQMAKTHGITREAQDALAHRSHSLASQAWKEGKIKDEVMTAFPAPYKKYLAED
NNIRHDSTVEGYAKLRPAFDRQYGSVTAANATPLTDGGAAVMLMREGKAKELGLEVLGYIRGYAFSAIGVETDMLMGPTY
ATSQVLKNTGLELSDLTLIEMHEAFAAQVLANVKMFASDEFAQKNLGRDKAIGEIDMEKFNVLGSSIAYGHPFAATGARM
MTQTLRELKRRGGGLALNTACAAGGLGAAMILEVE

Sequences:

>Translated_435_residues
MGKQEVKTRSGERVAVVAGLRTPFARQSTEFSQVPAVDLGKMVVSEMLARTDVDPALIEQVVFGQVVQMPEAPNIAREIV
LGTGMDINTDAYSVTRACATSFQAAVNVTESIMAGTIDVGIAGGADSSSVLPIGVSKKLAANLLALSKTKTIGQKLKILK
TFSVKDLMPVPPAVAEYSTGLSMGQTAEQMAKTHGITREAQDALAHRSHSLASQAWKEGKIKDEVMTAFPAPYKKYLAED
NNIRHDSTVEGYAKLRPAFDRQYGSVTAANATPLTDGGAAVMLMREGKAKELGLEVLGYIRGYAFSAIGVETDMLMGPTY
ATSQVLKNTGLELSDLTLIEMHEAFAAQVLANVKMFASDEFAQKNLGRDKAIGEIDMEKFNVLGSSIAYGHPFAATGARM
MTQTLRELKRRGGGLALNTACAAGGLGAAMILEVE
>Mature_434_residues
GKQEVKTRSGERVAVVAGLRTPFARQSTEFSQVPAVDLGKMVVSEMLARTDVDPALIEQVVFGQVVQMPEAPNIAREIVL
GTGMDINTDAYSVTRACATSFQAAVNVTESIMAGTIDVGIAGGADSSSVLPIGVSKKLAANLLALSKTKTIGQKLKILKT
FSVKDLMPVPPAVAEYSTGLSMGQTAEQMAKTHGITREAQDALAHRSHSLASQAWKEGKIKDEVMTAFPAPYKKYLAEDN
NIRHDSTVEGYAKLRPAFDRQYGSVTAANATPLTDGGAAVMLMREGKAKELGLEVLGYIRGYAFSAIGVETDMLMGPTYA
TSQVLKNTGLELSDLTLIEMHEAFAAQVLANVKMFASDEFAQKNLGRDKAIGEIDMEKFNVLGSSIAYGHPFAATGARMM
TQTLRELKRRGGGLALNTACAAGGLGAAMILEVE

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4504327, Length=420, Percent_Identity=41.1904761904762, Blast_Score=321, Evalue=6e-88,
Organism=Homo sapiens, GI4557237, Length=427, Percent_Identity=30.9133489461358, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI4501853, Length=430, Percent_Identity=30.4651162790698, Blast_Score=178, Evalue=7e-45,
Organism=Homo sapiens, GI167614485, Length=429, Percent_Identity=31.7016317016317, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI148539872, Length=435, Percent_Identity=30.5747126436782, Blast_Score=173, Evalue=3e-43,
Organism=Escherichia coli, GI1788683, Length=428, Percent_Identity=70.3271028037383, Blast_Score=608, Evalue=1e-175,
Organism=Escherichia coli, GI87082165, Length=423, Percent_Identity=33.3333333333333, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1788554, Length=428, Percent_Identity=33.6448598130841, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI48994986, Length=431, Percent_Identity=33.1786542923434, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1787663, Length=425, Percent_Identity=32, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17551802, Length=428, Percent_Identity=37.1495327102804, Blast_Score=277, Evalue=7e-75,
Organism=Caenorhabditis elegans, GI133906874, Length=428, Percent_Identity=32.4766355140187, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI25147385, Length=426, Percent_Identity=27.6995305164319, Blast_Score=148, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17535921, Length=428, Percent_Identity=28.5046728971963, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6322031, Length=451, Percent_Identity=31.0421286031042, Blast_Score=169, Evalue=9e-43,
Organism=Saccharomyces cerevisiae, GI6325229, Length=433, Percent_Identity=29.0993071593533, Blast_Score=164, Evalue=3e-41,
Organism=Drosophila melanogaster, GI17137578, Length=432, Percent_Identity=40.7407407407407, Blast_Score=318, Evalue=4e-87,
Organism=Drosophila melanogaster, GI24655093, Length=426, Percent_Identity=30.9859154929577, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI17648125, Length=428, Percent_Identity=28.5046728971963, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24640423, Length=428, Percent_Identity=27.3364485981308, Blast_Score=149, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012806
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 46260; Mature: 46129

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKQEVKTRSGERVAVVAGLRTPFARQSTEFSQVPAVDLGKMVVSEMLARTDVDPALIEQ
CCCHHHHHCCCCEEEEEEECCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCHHHHHH
VVFGQVVQMPEAPNIAREIVLGTGMDINTDAYSVTRACATSFQAAVNVTESIMAGTIDVG
HHHHHHHCCCCCCCHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
IAGGADSSSVLPIGVSKKLAANLLALSKTKTIGQKLKILKTFSVKDLMPVPPAVAEYSTG
EECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCC
LSMGQTAEQMAKTHGITREAQDALAHRSHSLASQAWKEGKIKDEVMTAFPAPYKKYLAED
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHCCC
NNIRHDSTVEGYAKLRPAFDRQYGSVTAANATPLTDGGAAVMLMREGKAKELGLEVLGYI
CCCCCCCCHHHHHHHCHHHHHCCCCEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHH
RGYAFSAIGVETDMLMGPTYATSQVLKNTGLELSDLTLIEMHEAFAAQVLANVKMFASDE
HHHHHHCCCCCHHHEECCCHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHCHH
FAQKNLGRDKAIGEIDMEKFNVLGSSIAYGHPFAATGARMMTQTLRELKRRGGGLALNTA
HHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHH
CAAGGLGAAMILEVE
HHCCCCCEEEEEEEC
>Mature Secondary Structure 
GKQEVKTRSGERVAVVAGLRTPFARQSTEFSQVPAVDLGKMVVSEMLARTDVDPALIEQ
CCHHHHHCCCCEEEEEEECCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCHHHHHH
VVFGQVVQMPEAPNIAREIVLGTGMDINTDAYSVTRACATSFQAAVNVTESIMAGTIDVG
HHHHHHHCCCCCCCHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
IAGGADSSSVLPIGVSKKLAANLLALSKTKTIGQKLKILKTFSVKDLMPVPPAVAEYSTG
EECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCC
LSMGQTAEQMAKTHGITREAQDALAHRSHSLASQAWKEGKIKDEVMTAFPAPYKKYLAED
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHCCC
NNIRHDSTVEGYAKLRPAFDRQYGSVTAANATPLTDGGAAVMLMREGKAKELGLEVLGYI
CCCCCCCCHHHHHHHCHHHHHCCCCEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHH
RGYAFSAIGVETDMLMGPTYATSQVLKNTGLELSDLTLIEMHEAFAAQVLANVKMFASDE
HHHHHHCCCCCHHHEECCCHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHCHH
FAQKNLGRDKAIGEIDMEKFNVLGSSIAYGHPFAATGARMMTQTLRELKRRGGGLALNTA
HHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHH
CAAGGLGAAMILEVE
HHCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]