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The map label for this gene is mlaA [H]

Identifier: 218708859

GI number: 218708859

Start: 878630

End: 879415

Strand: Direct

Name: mlaA [H]

Synonym: VS_0859

Alternate gene names: 218708859

Gene position: 878630-879415 (Clockwise)

Preceding gene: 218708858

Following gene: 218708864

Centisome position: 26.63

GC content: 43.13

Gene sequence:

>786_bases
ATGTCTATCAGTGTTTTAAGACTTTCGAGTTTACTCTTCATCGCAAGCTTAACGGTAGGTTGTTCGAGCGTACCAGATGA
AAGCAATGGCGGTGATAATTTCGAAACCTCTGAATATGTCGAAGAGTCCCATCCGAACGACCCTTTTGAAGGTTTCAACC
GAGCGATGTGGGATATCAACTACGAGTATCTCGACCCCTATTTGGTTCGACCTGTTTCTCTTGCCTATGTTGACTATACC
CCTGTACCAATTCGCTCTGGTATTTCCAATTTTTTAGCCAACTTAGATGAGCCATCAAGTATGCTCAATAATCTCATTAT
GGGTAATGGTGGGAAAGCGCTCGATCATTTCAATCGTTTTTGGATTAACTCTACCTTTGGTCTTCTTGGTCTGATTGATA
TTGCTAGCGAAGCGGGGATCACCAAATATGACGAAAAGTCGTTTTCTGATGCGATTGGTCATTATGGGGTAGGGAATGGA
CCGTATTTTATGCTGCCGGGATATGGCCCTGTGACGACCCGACAAGTAACAGAGCAAGTGGATAGCTTATATGTACCTTT
GTCTCTGTTTACCTTTTGGGCAAAGTTAGGGAAGTGGGCCTTTGAAGGTATGGAAACACGTGCTCAGTTGGCCTCGCAAG
AAGCCTTATTAGATGACTCTCCAGATCCATATGCTTTGACTCGTGATATTTACATCCAACGTCAAGATTTTAAAGCTGAG
ATCGAGCCAGAAGAGGTTGATCTTGAGGAAGAAGATTTCATTGATGAGTATCTCGAAGATTACTAG

Upstream 100 bases:

>100_bases
GTTAGCTATTCCGATTTATTAAAAAACGGTATAGACTTACGCAACCAATTGAGGCCAGCGATTGCTGGCCTTTTTTATTA
TTCCTTATGGAAAAGGTGAT

Downstream 100 bases:

>100_bases
AGACAAAGCTTAGATAAGCAGATAAGAGAAAGGCTCGATGTTAATTCAACATCGAGCCTTTTTAATGCTTTCTACTTTTC
TACCTTTCGGTAAGAAGGTA

Product: lipoprotein vacJ precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSISVLRLSSLLFIASLTVGCSSVPDESNGGDNFETSEYVEESHPNDPFEGFNRAMWDINYEYLDPYLVRPVSLAYVDYT
PVPIRSGISNFLANLDEPSSMLNNLIMGNGGKALDHFNRFWINSTFGLLGLIDIASEAGITKYDEKSFSDAIGHYGVGNG
PYFMLPGYGPVTTRQVTEQVDSLYVPLSLFTFWAKLGKWAFEGMETRAQLASQEALLDDSPDPYALTRDIYIQRQDFKAE
IEPEEVDLEEEDFIDEYLEDY

Sequences:

>Translated_261_residues
MSISVLRLSSLLFIASLTVGCSSVPDESNGGDNFETSEYVEESHPNDPFEGFNRAMWDINYEYLDPYLVRPVSLAYVDYT
PVPIRSGISNFLANLDEPSSMLNNLIMGNGGKALDHFNRFWINSTFGLLGLIDIASEAGITKYDEKSFSDAIGHYGVGNG
PYFMLPGYGPVTTRQVTEQVDSLYVPLSLFTFWAKLGKWAFEGMETRAQLASQEALLDDSPDPYALTRDIYIQRQDFKAE
IEPEEVDLEEEDFIDEYLEDY
>Mature_260_residues
SISVLRLSSLLFIASLTVGCSSVPDESNGGDNFETSEYVEESHPNDPFEGFNRAMWDINYEYLDPYLVRPVSLAYVDYTP
VPIRSGISNFLANLDEPSSMLNNLIMGNGGKALDHFNRFWINSTFGLLGLIDIASEAGITKYDEKSFSDAIGHYGVGNGP
YFMLPGYGPVTTRQVTEQVDSLYVPLSLFTFWAKLGKWAFEGMETRAQLASQEALLDDSPDPYALTRDIYIQRQDFKAEI
EPEEVDLEEEDFIDEYLEDY

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=246, Percent_Identity=42.2764227642276, Blast_Score=198, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 29396; Mature: 29265

Theoretical pI: Translated: 3.86; Mature: 3.86

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISVLRLSSLLFIASLTVGCSSVPDESNGGDNFETSEYVEESHPNDPFEGFNRAMWDIN
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCC
YEYLDPYLVRPVSLAYVDYTPVPIRSGISNFLANLDEPSSMLNNLIMGNGGKALDHFNRF
HHHCCHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHH
WINSTFGLLGLIDIASEAGITKYDEKSFSDAIGHYGVGNGPYFMLPGYGPVTTRQVTEQV
HHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHH
DSLYVPLSLFTFWAKLGKWAFEGMETRAQLASQEALLDDSPDPYALTRDIYIQRQDFKAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHEEEEEECCCCCC
IEPEEVDLEEEDFIDEYLEDY
CCHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SISVLRLSSLLFIASLTVGCSSVPDESNGGDNFETSEYVEESHPNDPFEGFNRAMWDIN
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCC
YEYLDPYLVRPVSLAYVDYTPVPIRSGISNFLANLDEPSSMLNNLIMGNGGKALDHFNRF
HHHCCHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHH
WINSTFGLLGLIDIASEAGITKYDEKSFSDAIGHYGVGNGPYFMLPGYGPVTTRQVTEQV
HHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHH
DSLYVPLSLFTFWAKLGKWAFEGMETRAQLASQEALLDDSPDPYALTRDIYIQRQDFKAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHEEEEEECCCCCC
IEPEEVDLEEEDFIDEYLEDY
CCHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]