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The map label for this gene is hisS [H]

Identifier: 218708642

GI number: 218708642

Start: 641943

End: 643211

Strand: Direct

Name: hisS [H]

Synonym: VS_0620

Alternate gene names: 218708642

Gene position: 641943-643211 (Clockwise)

Preceding gene: 218708641

Following gene: 218708643

Centisome position: 19.46

GC content: 47.68

Gene sequence:

>1269_bases
GTGGCTAAAAATATCCAAGCAATTCGAGGCATGAACGACTGCCTTCCAACTCAATCACCACTGTGGCAGAAAGTAGAAAG
CGCAGTTAAAAGTGTGGTGAGCGCATACGGTTACAACGAAGTACGTATGCCTATCGTTGAAGAGACGAACCTATTTAGCC
GTGCGGTTGGTGAAGAGACTGATGTTGTTTCTAAAGAGATGTACACCTTTGATGACCGCAATGGTGACAGCCTAACGCTG
CGTCCAGAAGGAACAGCAGGTTGTGTACGTTCATGTATTCAGAACAGCCTTATTAACCGTGATGAACAGCGCCTATGGTA
CATGGGCCCAATGTTCCGTCATGAGCGTCCTCAAAAAGGTCGTTACCGTCAATTCCACCAATGTGGCGTTGAAGTGTTTG
GCCTAGACGGTCCAGACGTTGACGCAGAACTTATTATGATGACAGCACGTCTATGGCGTGAACTAGGTATCGATAAGCAC
GTTCGTCTAGAACTGAACTCAATCGGTTCTCAAGAAGATCGCGTAAGCTACCGTACTGCGTTAGTGGCGTTCCTTGAGCA
ACACATCGATGTGCTAGATGAAGACTGCAAACGTCGCATGCACACTAACCCTCTACGTGTTCTGGATACTAAGAACCCGG
ATGTTCAAGCGATCTTAGGTGACGCCCCTCGCCTATCTGAATATCTAGGTGAAGAATCGAAGCAACATTTTGCTGGTTTG
TGTGAACTTCTTGACGCTGTTGGTATCGAATACCAAGTTAATGAGCGTCTAGTACGCGGCCTAGATTACTACAACCGCAC
CGTTTTTGAGTGGATCACAGACAGCCTTGGTGCCCAAGGTACAGTATGTGGTGGTGGCCGTTACGATGGTCTTGTTGAAC
AACTCGGCGGCAAAGCAACCAATGCTGTTGGCTTCGCTATGGGTCTAGAGCGTCTAGTCCTTATGATGGAAACGCTAGAG
CTTACAGAAGTTCGCCGTAGTGTTGACGTATACATGGTTGCTGCTGGCGAAGGTACAATGATCGCGGGCATGCAGTTAGC
GAATCAATTACGCGACACCGTTGAAGGTGTACGTGTGATGAACCACTTCGGTGGTGGTAACTTCAAGAAGCAATTCAAAC
GTGCTGACAAAGTAGGTGCTGTTGTGGCGTTGGTACTTGGTGAGAACGAAGTTGCTGACAATACAGTTGTGCTAAAAGAT
TTGGTTGGCGGCGAGCAACAAACCGTGTCTCAAACGGAAGTTGCAGAAAAAGTTGCTGCGTTAATCTAA

Upstream 100 bases:

>100_bases
CGCATTGATGTAGAAAACTTAGAAGATTAATACAACGCGATGAGGTGTGATTGTCTATTCGCACTGATACCTGGTCGTGA
GAAATTACGGAATAAATACT

Downstream 100 bases:

>100_bases
GCCATTGAGCAAACGCTCATCATGAATATCAACGTCAGCACTATGCTGGCGTTTAAAGAATTAAAGAGGACAGGAAGTGG
AACTTTACGATAGCGAAGAG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MAKNIQAIRGMNDCLPTQSPLWQKVESAVKSVVSAYGYNEVRMPIVEETNLFSRAVGEETDVVSKEMYTFDDRNGDSLTL
RPEGTAGCVRSCIQNSLINRDEQRLWYMGPMFRHERPQKGRYRQFHQCGVEVFGLDGPDVDAELIMMTARLWRELGIDKH
VRLELNSIGSQEDRVSYRTALVAFLEQHIDVLDEDCKRRMHTNPLRVLDTKNPDVQAILGDAPRLSEYLGEESKQHFAGL
CELLDAVGIEYQVNERLVRGLDYYNRTVFEWITDSLGAQGTVCGGGRYDGLVEQLGGKATNAVGFAMGLERLVLMMETLE
LTEVRRSVDVYMVAAGEGTMIAGMQLANQLRDTVEGVRVMNHFGGGNFKKQFKRADKVGAVVALVLGENEVADNTVVLKD
LVGGEQQTVSQTEVAEKVAALI

Sequences:

>Translated_422_residues
MAKNIQAIRGMNDCLPTQSPLWQKVESAVKSVVSAYGYNEVRMPIVEETNLFSRAVGEETDVVSKEMYTFDDRNGDSLTL
RPEGTAGCVRSCIQNSLINRDEQRLWYMGPMFRHERPQKGRYRQFHQCGVEVFGLDGPDVDAELIMMTARLWRELGIDKH
VRLELNSIGSQEDRVSYRTALVAFLEQHIDVLDEDCKRRMHTNPLRVLDTKNPDVQAILGDAPRLSEYLGEESKQHFAGL
CELLDAVGIEYQVNERLVRGLDYYNRTVFEWITDSLGAQGTVCGGGRYDGLVEQLGGKATNAVGFAMGLERLVLMMETLE
LTEVRRSVDVYMVAAGEGTMIAGMQLANQLRDTVEGVRVMNHFGGGNFKKQFKRADKVGAVVALVLGENEVADNTVVLKD
LVGGEQQTVSQTEVAEKVAALI
>Mature_421_residues
AKNIQAIRGMNDCLPTQSPLWQKVESAVKSVVSAYGYNEVRMPIVEETNLFSRAVGEETDVVSKEMYTFDDRNGDSLTLR
PEGTAGCVRSCIQNSLINRDEQRLWYMGPMFRHERPQKGRYRQFHQCGVEVFGLDGPDVDAELIMMTARLWRELGIDKHV
RLELNSIGSQEDRVSYRTALVAFLEQHIDVLDEDCKRRMHTNPLRVLDTKNPDVQAILGDAPRLSEYLGEESKQHFAGLC
ELLDAVGIEYQVNERLVRGLDYYNRTVFEWITDSLGAQGTVCGGGRYDGLVEQLGGKATNAVGFAMGLERLVLMMETLEL
TEVRRSVDVYMVAAGEGTMIAGMQLANQLRDTVEGVRVMNHFGGGNFKKQFKRADKVGAVVALVLGENEVADNTVVLKDL
VGGEQQTVSQTEVAEKVAALI

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=468, Percent_Identity=24.1452991452991, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1788861, Length=423, Percent_Identity=68.7943262411348, Blast_Score=614, Evalue=1e-177,
Organism=Caenorhabditis elegans, GI71993686, Length=468, Percent_Identity=24.7863247863248, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71993693, Length=468, Percent_Identity=24.7863247863248, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325290, Length=460, Percent_Identity=23.0434782608696, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24643061, Length=482, Percent_Identity=24.896265560166, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24643059, Length=501, Percent_Identity=23.9520958083832, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 47092; Mature: 46961

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNIQAIRGMNDCLPTQSPLWQKVESAVKSVVSAYGYNEVRMPIVEETNLFSRAVGEET
CCCCHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHCCCHH
DVVSKEMYTFDDRNGDSLTLRPEGTAGCVRSCIQNSLINRDEQRLWYMGPMFRHERPQKG
HHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHEEEECCHHHCCCCCCC
RYRQFHQCGVEVFGLDGPDVDAELIMMTARLWRELGIDKHVRLELNSIGSQEDRVSYRTA
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCHHHHHHHHH
LVAFLEQHIDVLDEDCKRRMHTNPLRVLDTKNPDVQAILGDAPRLSEYLGEESKQHFAGL
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCHHHHHHHCCHHHHHHHHH
CELLDAVGIEYQVNERLVRGLDYYNRTVFEWITDSLGAQGTVCGGGRYDGLVEQLGGKAT
HHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHCCCHH
NAVGFAMGLERLVLMMETLELTEVRRSVDVYMVAAGEGTMIAGMQLANQLRDTVEGVRVM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
NHFGGGNFKKQFKRADKVGAVVALVLGENEVADNTVVLKDLVGGEQQTVSQTEVAEKVAA
HHCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCEEHHHHHCCCHHHHHHHHHHHHHHH
LI
HC
>Mature Secondary Structure 
AKNIQAIRGMNDCLPTQSPLWQKVESAVKSVVSAYGYNEVRMPIVEETNLFSRAVGEET
CCCHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHCCCHH
DVVSKEMYTFDDRNGDSLTLRPEGTAGCVRSCIQNSLINRDEQRLWYMGPMFRHERPQKG
HHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHEEEECCHHHCCCCCCC
RYRQFHQCGVEVFGLDGPDVDAELIMMTARLWRELGIDKHVRLELNSIGSQEDRVSYRTA
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCHHHHHHHHH
LVAFLEQHIDVLDEDCKRRMHTNPLRVLDTKNPDVQAILGDAPRLSEYLGEESKQHFAGL
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCHHHHHHHCCHHHHHHHHH
CELLDAVGIEYQVNERLVRGLDYYNRTVFEWITDSLGAQGTVCGGGRYDGLVEQLGGKAT
HHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHCCCHH
NAVGFAMGLERLVLMMETLELTEVRRSVDVYMVAAGEGTMIAGMQLANQLRDTVEGVRVM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
NHFGGGNFKKQFKRADKVGAVVALVLGENEVADNTVVLKDLVGGEQQTVSQTEVAEKVAA
HHCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCEEHHHHHCCCHHHHHHHHHHHHHHH
LI
HC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA