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The map label for this gene is ispG [H]

Identifier: 218708641

GI number: 218708641

Start: 640751

End: 641872

Strand: Direct

Name: ispG [H]

Synonym: VS_0619

Alternate gene names: 218708641

Gene position: 640751-641872 (Clockwise)

Preceding gene: 218708640

Following gene: 218708642

Centisome position: 19.42

GC content: 46.52

Gene sequence:

>1122_bases
ATGCAACACGAATCTCCTATTATTCGTCGCAAATCAACCCGTATTTATGTGGGTGATGTGCCGATCGGTGATGGTGCACC
AATTGCTGTGCAATCCATGACCAACACAAGAACAACAGATGTAGCCGCGACCGTTGCTCAAATTAGAGCTCTGGAAAAAG
TTGGCGCTGATATCGTTCGCGTATCTGTACCGACTATGGATGCAGCTGAAGCCTTTAAGCTAATCAAGCAGCAGGTCTCT
GTTCCTTTGGTTGCTGATATTCACTTCGACTACCGTATCGCACTTAAAGTGGCTGAATACGGCGTTGACTGTCTGCGTAT
CAACCCAGGTAACATCGGTAACGAAAGCCGCATTCGCTCTGTTGTGGATTGTGCTCGTGATATGAATATCCCGATTCGTA
TTGGTGTTAACGGTGGTTCTCTTGAGAAAGAGATTCAAGAGAAATACACAGAGCCTACAGCAGAAGCGCTTGTTGAATCT
GCAATGCGTCACGTCGACATCCTAGACCGTTTGAACTTTGATCAATTTAAAGTCAGCGTTAAGGCGTCTGATGTATTCCT
AGCCGTCGGTTCTTACCGTTTACTTGCTAAGCAGATTGATCAACCTCTTCACCTTGGCATTACCGAAGCGGGTGGTGCGC
GTGCAGGCTCTGTGAAGTCAGCGGTAGGTTTAGGCATGCTTCTTTCTGAAGGTATCGGCGATACGCTGCGTATCTCGCTA
GCGGCTGATCCTGTTGAGGAGATCAAAGTTGGCTTTGATATTCTTAAATCTTTGCGCATTCGCTCGCGTGGTATTAACTT
CATCGCATGCCCGAGCTGTTCTCGTCAAGAGTTCGATGTTATTAACACCGTTAATGCCCTTGAAGAGCGCCTAGAAGACG
TGATTACTCCAATGGATGTCTCTATCATCGGTTGTGTGGTTAACGGCCCTGGTGAAGCTGAAGTGTCTCACTTAGGCCTA
GCTGGTAGTGCACGTAAGAGTGCTTTCTACGAAGACGGTAAGCGTCAGAAAGAGCGCTTCGACAACGATGATCTTGTCGA
CAAACTTGAAGCTAAGATTCGTGCAAAAGCATCAGTGCTTGATAAAGCAAATCGCATTGATGTAGAAAACTTAGAAGATT
AA

Upstream 100 bases:

>100_bases
GCGCCAGAGGGCGTATCGATGACATTTGCAAGTGAACCTGTCGACCTTTCTGGGTATACTTCAGGCAAAGTAGCTAGAAT
AACCTTACCTTAGAGTTAAT

Downstream 100 bases:

>100_bases
TACAACGCGATGAGGTGTGATTGTCTATTCGCACTGATACCTGGTCGTGAGAAATTACGGAATAAATACTGTGGCTAAAA
ATATCCAAGCAATTCGAGGC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MQHESPIIRRKSTRIYVGDVPIGDGAPIAVQSMTNTRTTDVAATVAQIRALEKVGADIVRVSVPTMDAAEAFKLIKQQVS
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNESRIRSVVDCARDMNIPIRIGVNGGSLEKEIQEKYTEPTAEALVES
AMRHVDILDRLNFDQFKVSVKASDVFLAVGSYRLLAKQIDQPLHLGITEAGGARAGSVKSAVGLGMLLSEGIGDTLRISL
AADPVEEIKVGFDILKSLRIRSRGINFIACPSCSRQEFDVINTVNALEERLEDVITPMDVSIIGCVVNGPGEAEVSHLGL
AGSARKSAFYEDGKRQKERFDNDDLVDKLEAKIRAKASVLDKANRIDVENLED

Sequences:

>Translated_373_residues
MQHESPIIRRKSTRIYVGDVPIGDGAPIAVQSMTNTRTTDVAATVAQIRALEKVGADIVRVSVPTMDAAEAFKLIKQQVS
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNESRIRSVVDCARDMNIPIRIGVNGGSLEKEIQEKYTEPTAEALVES
AMRHVDILDRLNFDQFKVSVKASDVFLAVGSYRLLAKQIDQPLHLGITEAGGARAGSVKSAVGLGMLLSEGIGDTLRISL
AADPVEEIKVGFDILKSLRIRSRGINFIACPSCSRQEFDVINTVNALEERLEDVITPMDVSIIGCVVNGPGEAEVSHLGL
AGSARKSAFYEDGKRQKERFDNDDLVDKLEAKIRAKASVLDKANRIDVENLED
>Mature_373_residues
MQHESPIIRRKSTRIYVGDVPIGDGAPIAVQSMTNTRTTDVAATVAQIRALEKVGADIVRVSVPTMDAAEAFKLIKQQVS
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNESRIRSVVDCARDMNIPIRIGVNGGSLEKEIQEKYTEPTAEALVES
AMRHVDILDRLNFDQFKVSVKASDVFLAVGSYRLLAKQIDQPLHLGITEAGGARAGSVKSAVGLGMLLSEGIGDTLRISL
AADPVEEIKVGFDILKSLRIRSRGINFIACPSCSRQEFDVINTVNALEERLEDVITPMDVSIIGCVVNGPGEAEVSHLGL
AGSARKSAFYEDGKRQKERFDNDDLVDKLEAKIRAKASVLDKANRIDVENLED

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=372, Percent_Identity=84.9462365591398, Blast_Score=645, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 40608; Mature: 40608

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHESPIIRRKSTRIYVGDVPIGDGAPIAVQSMTNTRTTDVAATVAQIRALEKVGADIVR
CCCCCCCCEECCCEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEE
VSVPTMDAAEAFKLIKQQVSVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNESRIRS
EECCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHCCCEEEEECCCCCCCHHHHHH
VVDCARDMNIPIRIGVNGGSLEKEIQEKYTEPTAEALVESAMRHVDILDRLNFDQFKVSV
HHHHHHCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
KASDVFLAVGSYRLLAKQIDQPLHLGITEAGGARAGSVKSAVGLGMLLSEGIGDTLRISL
EECEEEEECCHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEE
AADPVEEIKVGFDILKSLRIRSRGINFIACPSCSRQEFDVINTVNALEERLEDVITPMDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
SIIGCVVNGPGEAEVSHLGLAGSARKSAFYEDGKRQKERFDNDDLVDKLEAKIRAKASVL
EEEEEEECCCCCCCHHHCCCCCCHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHH
DKANRIDVENLED
HCCCCCCCCCCCC
>Mature Secondary Structure
MQHESPIIRRKSTRIYVGDVPIGDGAPIAVQSMTNTRTTDVAATVAQIRALEKVGADIVR
CCCCCCCCEECCCEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEE
VSVPTMDAAEAFKLIKQQVSVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNESRIRS
EECCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHCCCEEEEECCCCCCCHHHHHH
VVDCARDMNIPIRIGVNGGSLEKEIQEKYTEPTAEALVESAMRHVDILDRLNFDQFKVSV
HHHHHHCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
KASDVFLAVGSYRLLAKQIDQPLHLGITEAGGARAGSVKSAVGLGMLLSEGIGDTLRISL
EECEEEEECCHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEE
AADPVEEIKVGFDILKSLRIRSRGINFIACPSCSRQEFDVINTVNALEERLEDVITPMDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
SIIGCVVNGPGEAEVSHLGLAGSARKSAFYEDGKRQKERFDNDDLVDKLEAKIRAKASVL
EEEEEEECCCCCCCHHHCCCCCCHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHH
DKANRIDVENLED
HCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]