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The map label for this gene is rlmN

Identifier: 218708638

GI number: 218708638

Start: 638185

End: 639327

Strand: Direct

Name: rlmN

Synonym: VS_0616

Alternate gene names: 218708638

Gene position: 638185-639327 (Clockwise)

Preceding gene: 218708637

Following gene: 218708640

Centisome position: 19.34

GC content: 44.09

Gene sequence:

>1143_bases
ATGACCACAGCTAAAGTCAATCTACTCGATTTTGATCGTAAAGGTCTTCGTAAATTTTTCACAGAAGAACTGAATGAGAA
AGCGTTTCGAGCAGAGCAAGTGATGAAGTGGATTTATCACTTCGGTGTCGATGACTTCGAACAAATGAACAACATCAACA
AAAAGTTACGTGAGAAACTGCTGCATCGCTGTGAGATTGTTGCACCTATCGTTTCTGAAGCTCAGCACTCTGCGGATGGC
ACCATTAAATGGGCGATGAGCGTTGGTGACCAAGACGTTGAGACTGTATACATCCCAGATGGTGATCGTGCGACGCTATG
TGTATCTTCGCAAGTAGGTTGTGCGCTTGAATGTAAATTCTGCTCTACGGCTCAACAAGGCTTCAACCGTAACCTGAAAG
TTTCAGAGATCGTTGGTCAAATCTGGCGTGCTGCACGTGAAATCGGTTTAGAGAAAGAAACGGGTCGTCGTCCAATTACT
AACGTCGTAATGATGGGTATGGGTGAACCTCTATTGAACATGAAGAACCTAATTCCATCATTGGAGCTGATGCTTGATGA
TCTAGGTTTCTCACTGTCTAAGCGTCGTGTAACAGTATCAACATCTGGTGTTGTTTCTGGTCTTGATCAGATGACAGACA
ACATCGATGTAGCTCTGGCGATTTCTCTACACGCACCAAACGATGCACTACGTAGCCAAATCATGCCAATCAACGATCGT
TGGGATATCCAAGACTTCCTAGCATCGGTTCGTCGCTACATTGCTTCTTCAAATGCTAACCGCGGCAAAGTAACGGTTGA
GTACGTTCTGTTGGATCATGTGAATGATGATATGGACCACGCTCGTGAACTTGCTGAGCTAATGAAAGATACGCCTTGTA
AGATCAACTTGATTCCATTTAACCCTTATCCGGGTTCGCCTTATAAGAAGCCAAGCAACTCTCGTATTGATCGCTTCCAA
AAAACGCTGATGGAATACAACTACACAGTTACGGTTCGTAAAACTCGTGGTGATGATATTGATGCCGCATGTGGTCAGCT
GGTTGGTGATGTTATTGATAGAACCAAGCGTACTAAAATGCTGAAAGCCGCCTCTGAAGCTAACTTGATCGCAGGTGATG
TGATTCAAGTAAAAGCGGTTTAA

Upstream 100 bases:

>100_bases
GAATGTCTTAGTAAACACAAGGTTTTACAGTGCTTGTTGAAGTTGCGTAAAGGCTGTACAATTCGCGCCCTTAATTATTG
TTCCGTCATTGAGAGGCATC

Downstream 100 bases:

>100_bases
ATACCATTTAGATCTAAACAAGCCAGAAGGATTCCTTCTGGCTTGTTGCTTTTCTGGAAGGTGAATTTCCAATAATTGGA
TTTTTTCCCCATGCTTTCTT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MTTAKVNLLDFDRKGLRKFFTEELNEKAFRAEQVMKWIYHFGVDDFEQMNNINKKLREKLLHRCEIVAPIVSEAQHSADG
TIKWAMSVGDQDVETVYIPDGDRATLCVSSQVGCALECKFCSTAQQGFNRNLKVSEIVGQIWRAAREIGLEKETGRRPIT
NVVMMGMGEPLLNMKNLIPSLELMLDDLGFSLSKRRVTVSTSGVVSGLDQMTDNIDVALAISLHAPNDALRSQIMPINDR
WDIQDFLASVRRYIASSNANRGKVTVEYVLLDHVNDDMDHARELAELMKDTPCKINLIPFNPYPGSPYKKPSNSRIDRFQ
KTLMEYNYTVTVRKTRGDDIDAACGQLVGDVIDRTKRTKMLKAASEANLIAGDVIQVKAV

Sequences:

>Translated_380_residues
MTTAKVNLLDFDRKGLRKFFTEELNEKAFRAEQVMKWIYHFGVDDFEQMNNINKKLREKLLHRCEIVAPIVSEAQHSADG
TIKWAMSVGDQDVETVYIPDGDRATLCVSSQVGCALECKFCSTAQQGFNRNLKVSEIVGQIWRAAREIGLEKETGRRPIT
NVVMMGMGEPLLNMKNLIPSLELMLDDLGFSLSKRRVTVSTSGVVSGLDQMTDNIDVALAISLHAPNDALRSQIMPINDR
WDIQDFLASVRRYIASSNANRGKVTVEYVLLDHVNDDMDHARELAELMKDTPCKINLIPFNPYPGSPYKKPSNSRIDRFQ
KTLMEYNYTVTVRKTRGDDIDAACGQLVGDVIDRTKRTKMLKAASEANLIAGDVIQVKAV
>Mature_379_residues
TTAKVNLLDFDRKGLRKFFTEELNEKAFRAEQVMKWIYHFGVDDFEQMNNINKKLREKLLHRCEIVAPIVSEAQHSADGT
IKWAMSVGDQDVETVYIPDGDRATLCVSSQVGCALECKFCSTAQQGFNRNLKVSEIVGQIWRAAREIGLEKETGRRPITN
VVMMGMGEPLLNMKNLIPSLELMLDDLGFSLSKRRVTVSTSGVVSGLDQMTDNIDVALAISLHAPNDALRSQIMPINDRW
DIQDFLASVRRYIASSNANRGKVTVEYVLLDHVNDDMDHARELAELMKDTPCKINLIPFNPYPGSPYKKPSNSRIDRFQK
TLMEYNYTVTVRKTRGDDIDAACGQLVGDVIDRTKRTKMLKAASEANLIAGDVIQVKAV

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=377, Percent_Identity=67.6392572944297, Blast_Score=546, Evalue=1e-156,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_VIBSL (B7VJT5)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416259.1
- ProteinModelPortal:   B7VJT5
- SMR:   B7VJT5
- GeneID:   7160236
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0616
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK11194
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 42723; Mature: 42591

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAKVNLLDFDRKGLRKFFTEELNEKAFRAEQVMKWIYHFGVDDFEQMNNINKKLREKL
CCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LHRCEIVAPIVSEAQHSADGTIKWAMSVGDQDVETVYIPDGDRATLCVSSQVGCALECKF
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEECCCCCEEEEEECCCCCEEEEHH
CSTAQQGFNRNLKVSEIVGQIWRAAREIGLEKETGRRPITNVVMMGMGEPLLNMKNLIPS
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHCCCCHHHHHHHHHHH
LELMLDDLGFSLSKRRVTVSTSGVVSGLDQMTDNIDVALAISLHAPNDALRSQIMPINDR
HHHHHHHHCCCCCCCEEEEECCHHHHHHHHHCCCCEEEEEEEEECCCHHHHHCCCCCCCC
WDIQDFLASVRRYIASSNANRGKVTVEYVLLDHVNDDMDHARELAELMKDTPCKINLIPF
CCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEC
NPYPGSPYKKPSNSRIDRFQKTLMEYNYTVTVRKTRGDDIDAACGQLVGDVIDRTKRTKM
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LKAASEANLIAGDVIQVKAV
HHHHCCCCEEECCEEEEEEC
>Mature Secondary Structure 
TTAKVNLLDFDRKGLRKFFTEELNEKAFRAEQVMKWIYHFGVDDFEQMNNINKKLREKL
CCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LHRCEIVAPIVSEAQHSADGTIKWAMSVGDQDVETVYIPDGDRATLCVSSQVGCALECKF
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEECCCCCEEEEEECCCCCEEEEHH
CSTAQQGFNRNLKVSEIVGQIWRAAREIGLEKETGRRPITNVVMMGMGEPLLNMKNLIPS
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHCCCCHHHHHHHHHHH
LELMLDDLGFSLSKRRVTVSTSGVVSGLDQMTDNIDVALAISLHAPNDALRSQIMPINDR
HHHHHHHHCCCCCCCEEEEECCHHHHHHHHHCCCCEEEEEEEEECCCHHHHHCCCCCCCC
WDIQDFLASVRRYIASSNANRGKVTVEYVLLDHVNDDMDHARELAELMKDTPCKINLIPF
CCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEC
NPYPGSPYKKPSNSRIDRFQKTLMEYNYTVTVRKTRGDDIDAACGQLVGDVIDRTKRTKM
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LKAASEANLIAGDVIQVKAV
HHHHCCCCEEECCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA