Click here to switch to the map view.

The map label for this gene is ptsP [H]

Identifier: 218708558

GI number: 218708558

Start: 542198

End: 544447

Strand: Direct

Name: ptsP [H]

Synonym: VS_0524

Alternate gene names: 218708558

Gene position: 542198-544447 (Clockwise)

Preceding gene: 218708557

Following gene: 218708559

Centisome position: 16.43

GC content: 45.24

Gene sequence:

>2250_bases
ATGCTCAGCCAACTAAGGGAAATAGTTGAACACGTATCAAGAGTTGAAGATGTGTCGACGGCTCTTGATATTTTGGTTAA
AGAGACATGCAGCGCGATGCAGACGGAATGTTGCACCGTGTATTTGGCAAATAATGATATGCAGCGTCTTGAGTTGATGG
CAACTCAAGGCCTGATCTTCAAAGGCAATAGTATTCACATTGGTTTTGACGAAGGTTTGGTTGGCCTTGTTAAAAGAAGT
GCTGAACCTATTAACCTTGCACAGGCGTCTGCTCACCCTGCTTATAAGTTTTTTCCCGAGCTTGGAGAAAAGGTTTATCA
CTCTTTTCTAGGCACTCCGATTATCTATCGCAAGCAAGTGCTTGGCGTATTGGTCATTCAACAGAAGTCCCCCCGTTTAT
TCAGTGAGATGGAAGAATCCTTCCTTGTTACACTCTCAGCACAACTTGCGGTTATTGTGGCGCATGCCCAAACTCAAGGC
CATTGGCTGTTAGAGCAACAGAAGCTACCTGCGACCAAAGGTATTGCAGCTTCATCGGGTGTGGCGATTGGTGACTTATG
GTGGGACAACACTCAGCCTGAACTCACCGATGTTTACCCTGCTTCGACGCTCAATGTTGAAAGGGAGCATGAGCTGTTGG
CAGTCGCTGTCGAAAATGCTCTCAATGATTTCAAGCGAATGCGAAAGCGTTTAGACAGTGAAATCAATAAAGACGCGTTG
GCGATCTTTGATCTGTTTACTCACTTACTCAACGATCCGATGTTACGTAAGGATCTCAAGAGCCAAATTCAGAAAGGCGA
CAAAGCCGATTGGGCATTGAGACAGGTTGTTGAGAGTTACTCTAACCGTTTTGCTCGTATGTCAGATGTGTACCTTCGGG
AGAGAGCGCAAGACGTCCGAGAGCTTGGTCAAAGGCTGCTCTATTTCCTCCACAATTCTGAACATGAACTTCGCACGTTA
GATAAACCGATTATTTTGGTGGTTCGTGAGTTAACGGCCTCTGTATTAGCGTCTATTCCTAAAGAAAAACTATTAGCGGT
TATTTCCTTGGAAGGGGCGGCGAACTCGCACGCAGCGATTTTATCTCGAGCTTTGGGTATCCCTTCTGTTATGGGGGTTA
ACCTTAATCTTTCGCAAGCTAATGGTAAGCAAGCGATTGTTGACGGGTATAGCGGCGAGATCTTTATTGAGCCGACTAAA
AACCTGCTCAAAGAGTATCGGGGGCTGATTCTAGAAGAGAGTGAGCTCTCCTCTATGGTCAATCGAGAACTGTACTTACC
GGCAAAAACGAAAGACGACAAGCAAGTCGAGATTCTGCTTAATGCGGGTTTGAGTGCAGACACTAACATCGCTATCAACC
AAGGGGTTGATGGGGTTGGTCTGTATCGAACCGAAATTTCTTTCTTGTTGCAGCAAAGGTTTCCGTCAGAGGACGAACAA
TTCAAACAGTATCGTTCTGTGCTTGCGAGTTACCCTGAGAAGCAAGTGGTGATGCGAACCTTGGATATTGGTGGAGATAA
GGCGTTACCTTACTTCCCAATAGAAGAAGATAATCCATTCCTTGGTTGGCGTGGTATTCGTTTTACCCTTGATCACCCCG
ACATCTTTATTATTCAGTTACGTGCGATGCTACGTGCGAGCTGTGAAAGCCATAACTTGAGTATCTTGCTGCCGATGATT
TCTGGTGCGCAGGAGCTGGATGACGCGTTCCAACTGATTGAACAAGCGTATGACGAAGTGCATGAGCTTGATAACCGAGT
GCGTATGCCGCGTATCGGTATCATGATTGAAGTGCCATCCATGCTTTACCTTCTTCCGCTTATTGCGGATAAAGTTGATT
TCGTTTCGGTGGGCACCAATGACTTAACTCAATATTTATTGGCGGTTGACCGGAACAATTCCCGTGTCTCTGATGTCTAT
GAATCCATGCACCCTGCAGTGATCATGGCATTGAAACATATCCATGATACTTGTAAGCAATATCAATTGCCGGTGTGTAT
ATGTGGAGAGCTTGCGGGTGATCCAATGGGTGCATTGCTCTTGATTGGGTTAGGGTATGAGACGTTAAGTATGAACACCT
CAAATGTCGCAAGGACTAAGTATTTGATTCGTCAATCTAAGCTTAGTGAACTGCAAGATTTGGCAAATGAGGCGCTATCA
AAACCGTATGGAAGTGACATCTATAGTATGATGCTCAACTATTTCGAAGAGCGTGAATTTACAGGCTTCATTCGAGCGGG
TAAAAAATAG

Upstream 100 bases:

>100_bases
GAGATGTTTACCGTCGAGCGATGAAAGAATTCGCATCTTTAGCAATGCCGTTTAAAGAGCGAAAAACAAAAGGAAAGCGC
AAATTGCGTAGAGGTTAAGC

Downstream 100 bases:

>100_bases
GATTTAACGTGTCATATGAACTGACAGGCTTGCTAGCAGGCCTTGGTGCTATTGTTGGTGTTTTAGCTGGCTTGCTAGGC
ATTGGTGGTGGTTTGCTGGT

Product: fused phosphoenolpyruvate-protein phosphotransferase PtsP/GAF domain

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 749; Mature: 749

Protein sequence:

>749_residues
MLSQLREIVEHVSRVEDVSTALDILVKETCSAMQTECCTVYLANNDMQRLELMATQGLIFKGNSIHIGFDEGLVGLVKRS
AEPINLAQASAHPAYKFFPELGEKVYHSFLGTPIIYRKQVLGVLVIQQKSPRLFSEMEESFLVTLSAQLAVIVAHAQTQG
HWLLEQQKLPATKGIAASSGVAIGDLWWDNTQPELTDVYPASTLNVEREHELLAVAVENALNDFKRMRKRLDSEINKDAL
AIFDLFTHLLNDPMLRKDLKSQIQKGDKADWALRQVVESYSNRFARMSDVYLRERAQDVRELGQRLLYFLHNSEHELRTL
DKPIILVVRELTASVLASIPKEKLLAVISLEGAANSHAAILSRALGIPSVMGVNLNLSQANGKQAIVDGYSGEIFIEPTK
NLLKEYRGLILEESELSSMVNRELYLPAKTKDDKQVEILLNAGLSADTNIAINQGVDGVGLYRTEISFLLQQRFPSEDEQ
FKQYRSVLASYPEKQVVMRTLDIGGDKALPYFPIEEDNPFLGWRGIRFTLDHPDIFIIQLRAMLRASCESHNLSILLPMI
SGAQELDDAFQLIEQAYDEVHELDNRVRMPRIGIMIEVPSMLYLLPLIADKVDFVSVGTNDLTQYLLAVDRNNSRVSDVY
ESMHPAVIMALKHIHDTCKQYQLPVCICGELAGDPMGALLLIGLGYETLSMNTSNVARTKYLIRQSKLSELQDLANEALS
KPYGSDIYSMMLNYFEEREFTGFIRAGKK

Sequences:

>Translated_749_residues
MLSQLREIVEHVSRVEDVSTALDILVKETCSAMQTECCTVYLANNDMQRLELMATQGLIFKGNSIHIGFDEGLVGLVKRS
AEPINLAQASAHPAYKFFPELGEKVYHSFLGTPIIYRKQVLGVLVIQQKSPRLFSEMEESFLVTLSAQLAVIVAHAQTQG
HWLLEQQKLPATKGIAASSGVAIGDLWWDNTQPELTDVYPASTLNVEREHELLAVAVENALNDFKRMRKRLDSEINKDAL
AIFDLFTHLLNDPMLRKDLKSQIQKGDKADWALRQVVESYSNRFARMSDVYLRERAQDVRELGQRLLYFLHNSEHELRTL
DKPIILVVRELTASVLASIPKEKLLAVISLEGAANSHAAILSRALGIPSVMGVNLNLSQANGKQAIVDGYSGEIFIEPTK
NLLKEYRGLILEESELSSMVNRELYLPAKTKDDKQVEILLNAGLSADTNIAINQGVDGVGLYRTEISFLLQQRFPSEDEQ
FKQYRSVLASYPEKQVVMRTLDIGGDKALPYFPIEEDNPFLGWRGIRFTLDHPDIFIIQLRAMLRASCESHNLSILLPMI
SGAQELDDAFQLIEQAYDEVHELDNRVRMPRIGIMIEVPSMLYLLPLIADKVDFVSVGTNDLTQYLLAVDRNNSRVSDVY
ESMHPAVIMALKHIHDTCKQYQLPVCICGELAGDPMGALLLIGLGYETLSMNTSNVARTKYLIRQSKLSELQDLANEALS
KPYGSDIYSMMLNYFEEREFTGFIRAGKK
>Mature_749_residues
MLSQLREIVEHVSRVEDVSTALDILVKETCSAMQTECCTVYLANNDMQRLELMATQGLIFKGNSIHIGFDEGLVGLVKRS
AEPINLAQASAHPAYKFFPELGEKVYHSFLGTPIIYRKQVLGVLVIQQKSPRLFSEMEESFLVTLSAQLAVIVAHAQTQG
HWLLEQQKLPATKGIAASSGVAIGDLWWDNTQPELTDVYPASTLNVEREHELLAVAVENALNDFKRMRKRLDSEINKDAL
AIFDLFTHLLNDPMLRKDLKSQIQKGDKADWALRQVVESYSNRFARMSDVYLRERAQDVRELGQRLLYFLHNSEHELRTL
DKPIILVVRELTASVLASIPKEKLLAVISLEGAANSHAAILSRALGIPSVMGVNLNLSQANGKQAIVDGYSGEIFIEPTK
NLLKEYRGLILEESELSSMVNRELYLPAKTKDDKQVEILLNAGLSADTNIAINQGVDGVGLYRTEISFLLQQRFPSEDEQ
FKQYRSVLASYPEKQVVMRTLDIGGDKALPYFPIEEDNPFLGWRGIRFTLDHPDIFIIQLRAMLRASCESHNLSILLPMI
SGAQELDDAFQLIEQAYDEVHELDNRVRMPRIGIMIEVPSMLYLLPLIADKVDFVSVGTNDLTQYLLAVDRNNSRVSDVY
ESMHPAVIMALKHIHDTCKQYQLPVCICGELAGDPMGALLLIGLGYETLSMNTSNVARTKYLIRQSKLSELQDLANEALS
KPYGSDIYSMMLNYFEEREFTGFIRAGKK

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=749, Percent_Identity=48.5981308411215, Blast_Score=732, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=578, Percent_Identity=34.9480968858132, Blast_Score=312, Evalue=5e-86,
Organism=Escherichia coli, GI48994992, Length=488, Percent_Identity=32.3770491803279, Blast_Score=246, Evalue=4e-66,
Organism=Escherichia coli, GI1788726, Length=579, Percent_Identity=31.6062176165803, Blast_Score=246, Evalue=4e-66,
Organism=Escherichia coli, GI1787994, Length=457, Percent_Identity=25.164113785558, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI226510935, Length=177, Percent_Identity=29.9435028248588, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 84240; Mature: 84240

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00063 ALDOKETO_REDUCTASE_3 ; PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSQLREIVEHVSRVEDVSTALDILVKETCSAMQTECCTVYLANNDMQRLELMATQGLIF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCEEE
KGNSIHIGFDEGLVGLVKRSAEPINLAQASAHPAYKFFPELGEKVYHSFLGTPIIYRKQV
ECCEEEEECCCHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
LGVLVIQQKSPRLFSEMEESFLVTLSAQLAVIVAHAQTQGHWLLEQQKLPATKGIAASSG
HHEEEECCCCCHHHHHHHHHHEEEEECCEEEEEEECCCCCCEEEECCCCCCCCCCCCCCC
VAIGDLWWDNTQPELTDVYPASTLNVEREHELLAVAVENALNDFKRMRKRLDSEINKDAL
CEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
AIFDLFTHLLNDPMLRKDLKSQIQKGDKADWALRQVVESYSNRFARMSDVYLRERAQDVR
HHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGQRLLYFLHNSEHELRTLDKPIILVVRELTASVLASIPKEKLLAVISLEGAANSHAAI
HHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCCCHHHH
LSRALGIPSVMGVNLNLSQANGKQAIVDGYSGEIFIEPTKNLLKEYRGLILEESELSSMV
HHHHHCCCCEEECEEEEECCCCCEEEEECCCCEEEECCHHHHHHHHHCCEECHHHHHHHH
NRELYLPAKTKDDKQVEILLNAGLSADTNIAINQGVDGVGLYRTEISFLLQQRFPSEDEQ
CCEEECCCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCHHH
FKQYRSVLASYPEKQVVMRTLDIGGDKALPYFPIEEDNPFLGWRGIRFTLDHPDIFIIQL
HHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECEEEEECCCCEEHHHH
RAMLRASCESHNLSILLPMISGAQELDDAFQLIEQAYDEVHELDNRVRMPRIGIMIEVPS
HHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHH
MLYLLPLIADKVDFVSVGTNDLTQYLLAVDRNNSRVSDVYESMHPAVIMALKHIHDTCKQ
HHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YQLPVCICGELAGDPMGALLLIGLGYETLSMNTSNVARTKYLIRQSKLSELQDLANEALS
CCCCEEEECCCCCCCCCCHHEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
KPYGSDIYSMMLNYFEEREFTGFIRAGKK
CCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLSQLREIVEHVSRVEDVSTALDILVKETCSAMQTECCTVYLANNDMQRLELMATQGLIF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCEEE
KGNSIHIGFDEGLVGLVKRSAEPINLAQASAHPAYKFFPELGEKVYHSFLGTPIIYRKQV
ECCEEEEECCCHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
LGVLVIQQKSPRLFSEMEESFLVTLSAQLAVIVAHAQTQGHWLLEQQKLPATKGIAASSG
HHEEEECCCCCHHHHHHHHHHEEEEECCEEEEEEECCCCCCEEEECCCCCCCCCCCCCCC
VAIGDLWWDNTQPELTDVYPASTLNVEREHELLAVAVENALNDFKRMRKRLDSEINKDAL
CEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
AIFDLFTHLLNDPMLRKDLKSQIQKGDKADWALRQVVESYSNRFARMSDVYLRERAQDVR
HHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGQRLLYFLHNSEHELRTLDKPIILVVRELTASVLASIPKEKLLAVISLEGAANSHAAI
HHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCCCHHHH
LSRALGIPSVMGVNLNLSQANGKQAIVDGYSGEIFIEPTKNLLKEYRGLILEESELSSMV
HHHHHCCCCEEECEEEEECCCCCEEEEECCCCEEEECCHHHHHHHHHCCEECHHHHHHHH
NRELYLPAKTKDDKQVEILLNAGLSADTNIAINQGVDGVGLYRTEISFLLQQRFPSEDEQ
CCEEECCCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCHHH
FKQYRSVLASYPEKQVVMRTLDIGGDKALPYFPIEEDNPFLGWRGIRFTLDHPDIFIIQL
HHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECEEEEECCCCEEHHHH
RAMLRASCESHNLSILLPMISGAQELDDAFQLIEQAYDEVHELDNRVRMPRIGIMIEVPS
HHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHH
MLYLLPLIADKVDFVSVGTNDLTQYLLAVDRNNSRVSDVYESMHPAVIMALKHIHDTCKQ
HHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YQLPVCICGELAGDPMGALLLIGLGYETLSMNTSNVARTKYLIRQSKLSELQDLANEALS
CCCCEEEECCCCCCCCCCHHEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
KPYGSDIYSMMLNYFEEREFTGFIRAGKK
CCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]