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The map label for this gene is carA [H]

Identifier: 218708497

GI number: 218708497

Start: 474714

End: 475868

Strand: Direct

Name: carA [H]

Synonym: VS_0461

Alternate gene names: 218708497

Gene position: 474714-475868 (Clockwise)

Preceding gene: 218708496

Following gene: 218708498

Centisome position: 14.39

GC content: 46.41

Gene sequence:

>1155_bases
TTGAAGAAGTCAGCACTACTCGTCCTAGAAGATGGGACAGTATTTCACGGTGAAGCCATTGGCGCTGTAGGTTCTGCAGT
TGGTGAAGTCGTTTTTAATACCTCGATGACGGGGTACCAAGAAATCCTCACTGATCCTTCCTATTCTCAACAAATCGTTA
CCCTTACTTACCCTCACATTGGCAATACCGGAACCAATTCCGAAGACGAAGAATCTTCTTCAATCCACGCTCAAGGCCTT
GTGATTCGCGATCTCCCTCTAATCGCTTCTAACTTCCGTAATGAACAATCCCTTTCTGATTACCTTAAGTCGCAAAACGT
TGTTGGTATCGCTGATATCGATACTCGTAAGCTGACGCGTATTCTTCGTGAAAAAGGCGCACAGAACGGTTGTATCGTAG
CTGGCTCTTCAGTAAATGATGGAAATTTAGACGAAGCTTTGGCTCTAGCTAAAGCAAAAGAATTCCCTGGCCTGAAAGGT
ATGGACCTTGCGAAAGAAGTTACAACAAAAGAAGCGTATCAATGGAAACAAGGTTCGTGGACGCTTACGGGTGGACTTCC
TGAAGCGAAAGCAGACTCAGAATTGCCATACCACGTTGTTGCTTATGACTTCGGTGCAAAACGAAACATCCTACGAATGC
TCGTTGACCGCGGCTGCCGCCTAACGGTTGTTCCTGCTGAGACTTCAGCTGAAGAAGTACTTGCTCTGAACCCAGACGGT
GTTTTCCTTTCAAATGGCCCTGGTGACCCAGAACCATGTACTTACGCAATTGAAGCGACAAAAGTGTTCCTAGAAAAAGG
CTTACCAATCTTCGGTATCTGTCTAGGTCACCAGATTCTTGCTCTTGCGTCAGGCGCTAAGACAGTGAAGATGAAGTTTG
GTCACCACGGTGCAAACCACCCAGTGAAAGATTTAGATCGTGATGTTGTGATGATTACTTCGCAAAACCACGGCTTTGCT
GCTGACGAAGAAACCCTTCCAGAGACACTACGTGCAACGCACAAATCACTATTTGATGGTTCTCTACAAGGTATTCACCG
TACAGACAAACCAGCATTCAGCTTCCAAGGTCACCCTGAAGCGAGCCCAGGTCCAGAAGACGCAGCGCCGTTATTCGACC
ACTTTATTGAACTAATCAAACAGCACACAGCGTAA

Upstream 100 bases:

>100_bases
AATTTGTCTAATTTCCATAAACACGCAGTTTTTGGTGTTGCAGGAAGGTAGATTTGCAAATTAAATCAATTTTAATGCAT
TTTTATTTCTGGAGGTTGTC

Downstream 100 bases:

>100_bases
TTCGGAGTAGTAGACAATGCCAAAACGTACTGACATTAAAAGTATTCTTATTCTAGGTGCTGGCCCGATTGTTATCGGCC
AAGCATGTGAGTTCGATTAC

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MKKSALLVLEDGTVFHGEAIGAVGSAVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHIGNTGTNSEDEESSSIHAQGL
VIRDLPLIASNFRNEQSLSDYLKSQNVVGIADIDTRKLTRILREKGAQNGCIVAGSSVNDGNLDEALALAKAKEFPGLKG
MDLAKEVTTKEAYQWKQGSWTLTGGLPEAKADSELPYHVVAYDFGAKRNILRMLVDRGCRLTVVPAETSAEEVLALNPDG
VFLSNGPGDPEPCTYAIEATKVFLEKGLPIFGICLGHQILALASGAKTVKMKFGHHGANHPVKDLDRDVVMITSQNHGFA
ADEETLPETLRATHKSLFDGSLQGIHRTDKPAFSFQGHPEASPGPEDAAPLFDHFIELIKQHTA

Sequences:

>Translated_384_residues
MKKSALLVLEDGTVFHGEAIGAVGSAVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHIGNTGTNSEDEESSSIHAQGL
VIRDLPLIASNFRNEQSLSDYLKSQNVVGIADIDTRKLTRILREKGAQNGCIVAGSSVNDGNLDEALALAKAKEFPGLKG
MDLAKEVTTKEAYQWKQGSWTLTGGLPEAKADSELPYHVVAYDFGAKRNILRMLVDRGCRLTVVPAETSAEEVLALNPDG
VFLSNGPGDPEPCTYAIEATKVFLEKGLPIFGICLGHQILALASGAKTVKMKFGHHGANHPVKDLDRDVVMITSQNHGFA
ADEETLPETLRATHKSLFDGSLQGIHRTDKPAFSFQGHPEASPGPEDAAPLFDHFIELIKQHTA
>Mature_384_residues
MKKSALLVLEDGTVFHGEAIGAVGSAVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHIGNTGTNSEDEESSSIHAQGL
VIRDLPLIASNFRNEQSLSDYLKSQNVVGIADIDTRKLTRILREKGAQNGCIVAGSSVNDGNLDEALALAKAKEFPGLKG
MDLAKEVTTKEAYQWKQGSWTLTGGLPEAKADSELPYHVVAYDFGAKRNILRMLVDRGCRLTVVPAETSAEEVLALNPDG
VFLSNGPGDPEPCTYAIEATKVFLEKGLPIFGICLGHQILALASGAKTVKMKFGHHGANHPVKDLDRDVVMITSQNHGFA
ADEETLPETLRATHKSLFDGSLQGIHRTDKPAFSFQGHPEASPGPEDAAPLFDHFIELIKQHTA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=391, Percent_Identity=39.386189258312, Blast_Score=241, Evalue=7e-64,
Organism=Homo sapiens, GI169790915, Length=395, Percent_Identity=35.6962025316456, Blast_Score=215, Evalue=6e-56,
Organism=Homo sapiens, GI21361331, Length=395, Percent_Identity=35.6962025316456, Blast_Score=215, Evalue=7e-56,
Organism=Escherichia coli, GI1786215, Length=382, Percent_Identity=81.413612565445, Blast_Score=659, Evalue=0.0,
Organism=Caenorhabditis elegans, GI193204318, Length=390, Percent_Identity=37.9487179487179, Blast_Score=234, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6324878, Length=396, Percent_Identity=39.3939393939394, Blast_Score=239, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6322331, Length=395, Percent_Identity=36.4556962025316, Blast_Score=225, Evalue=9e-60,
Organism=Drosophila melanogaster, GI45555749, Length=394, Percent_Identity=39.8477157360406, Blast_Score=238, Evalue=4e-63,
Organism=Drosophila melanogaster, GI24642586, Length=394, Percent_Identity=39.8477157360406, Blast_Score=238, Evalue=5e-63,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41389; Mature: 41389

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSALLVLEDGTVFHGEAIGAVGSAVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHI
CCCCEEEEEECCCEEECHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEEECCCC
GNTGTNSEDEESSSIHAQGLVIRDLPLIASNFRNEQSLSDYLKSQNVVGIADIDTRKLTR
CCCCCCCCCCCCCCEEECCEEEECCHHHHHHCCCHHHHHHHHHCCCEEEEECCCHHHHHH
ILREKGAQNGCIVAGSSVNDGNLDEALALAKAKEFPGLKGMDLAKEVTTKEAYQWKQGSW
HHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCCE
TLTGGLPEAKADSELPYHVVAYDFGAKRNILRMLVDRGCRLTVVPAETSAEEVLALNPDG
EEECCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCC
VFLSNGPGDPEPCTYAIEATKVFLEKGLPIFGICLGHQILALASGAKTVKMKFGHHGANH
EEECCCCCCCCCEEEEHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCC
PVKDLDRDVVMITSQNHGFAADEETLPETLRATHKSLFDGSLQGIHRTDKPAFSFQGHPE
CHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCC
ASPGPEDAAPLFDHFIELIKQHTA
CCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKSALLVLEDGTVFHGEAIGAVGSAVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHI
CCCCEEEEEECCCEEECHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEEECCCC
GNTGTNSEDEESSSIHAQGLVIRDLPLIASNFRNEQSLSDYLKSQNVVGIADIDTRKLTR
CCCCCCCCCCCCCCEEECCEEEECCHHHHHHCCCHHHHHHHHHCCCEEEEECCCHHHHHH
ILREKGAQNGCIVAGSSVNDGNLDEALALAKAKEFPGLKGMDLAKEVTTKEAYQWKQGSW
HHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCCE
TLTGGLPEAKADSELPYHVVAYDFGAKRNILRMLVDRGCRLTVVPAETSAEEVLALNPDG
EEECCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCC
VFLSNGPGDPEPCTYAIEATKVFLEKGLPIFGICLGHQILALASGAKTVKMKFGHHGANH
EEECCCCCCCCCEEEEHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCC
PVKDLDRDVVMITSQNHGFAADEETLPETLRATHKSLFDGSLQGIHRTDKPAFSFQGHPE
CHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCC
ASPGPEDAAPLFDHFIELIKQHTA
CCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA