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The map label for this gene is murD

Identifier: 218708484

GI number: 218708484

Start: 458580

End: 459896

Strand: Direct

Name: murD

Synonym: VS_0448

Alternate gene names: 218708484

Gene position: 458580-459896 (Clockwise)

Preceding gene: 218708483

Following gene: 218708485

Centisome position: 13.9

GC content: 46.55

Gene sequence:

>1317_bases
ATGGAACGTTGGCAAAATATTCAAAATGTAGTGGTTGTGGGGCTCGGTATTACCGGGCTCTCTGTCGTTAAACATCTCGT
AAAATATCAACCTCAGACTCATGTGAGAGTGATTGATACACGAGAACTACCACCAGGAAAGGAATCTTTGCCTGAATCGG
TAGAACTGCATTCTGGAAGTTGGAATAGCCAATGGTTAGCGGAAGCTGATTTAGTGGTTGCTAACCCAGGTATCGCCTTA
GCGACCTCTGAAATACAAGATGTTATTCAAGCAGGAACTCCGGTTGTTGGCGATATTGAACTGTTTGGTTGGGCGGTAAA
CAAACCCGCCGTGGCCATAACAGGCTCCAATGGCAAGAGTACGGTGACCGATCTAACGGGTGTGCTTGCTAAAGCTGCTG
GTCTTAACGTTGGCGTTGGTGGCAACATCGGTATTCCTGCCTTGGATCTTCTTGAGCTTGATGCCGATTTATATGTCCTT
GAGCTTTCAAGCTTTCAGCTAGAGACAACGTCCAGTCTAAACCTTGCAGCAGCGGCCTTTTTGAACCTGTCAGAAGATCA
TATGGATCGCTATCAAGGCATGGCCGATTACCGTGACGCTAAATTGAGAATTTTTAATAACGCTCAGTACGCGATTGTAA
ACCGCGAAGACAAAGAGACTTATCCTGACCACAGTATGCCATTAGTGACATTCGGACTCGACGACCAAGAGTTTGGTGTG
GCGACGATTGATGGTACTGAATGGCTAACGGACAACGGCAAGCCTGTACTTCCTAGCCAAGATTTAACATTGGTTGGACG
TCATAATGTGGCGAATGCTTTAGTCTCGTTAGCGTTATTGAAGCAAGTCGGTATTGATTACAACAAAAGCCTTGAAGCTC
TGAAAGCCTACAATGGTTTAACGCATCGCTGCCAAGTAGTGGCTAACAAACGCGAAATCAAATGGGTTAACGACTCGAAA
GCGACCAACGTAGCCAGTACATTAGCGGCTCTGTCGGGGTTAGAGTACCAAGGTACTTTGTATCTCTTAGTCGGAGGTGT
GGGCAAAGGCGCTGACTTTAGCGAGCTTAAGCCTGTGTTGGCGCAACTAGACCGCGTACAACTGTGTTGCTTCGGTGAAG
ATGCTGCGCAATTTATGCCATTACATCCATCAGCTAAGACGTTTGATACCATGCGTGACATCATCGAGAGTATCTCTGCA
CAATTGGTGTCGGGTGACATGGTGATGTTGTCTCCTGCATGTGCAAGCTTCGATCAATTTAATAACTTCATGGCGAGAGG
TGATGCGTTCACTGAACTTGCTCATGAGTATGCCTAA

Upstream 100 bases:

>100_bases
GTATTAGTACTGGTTGGCCTAGCAACGCTGAAAGTTCGTTAATCCTATGTGAGCCTCTTAATTAGAGGCTCTTTAAAACT
ATTAAGAGCATCTTGTTTAA

Downstream 100 bases:

>100_bases
CGTGTTGAAGGACTAACATTCAGTGCAAAGAGTGAAAGAGATTAATCAATCTATTTGGCAATGGCTTAACCGTGCCACAC
CAGAGGCGCTCTACGATCGT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MERWQNIQNVVVVGLGITGLSVVKHLVKYQPQTHVRVIDTRELPPGKESLPESVELHSGSWNSQWLAEADLVVANPGIAL
ATSEIQDVIQAGTPVVGDIELFGWAVNKPAVAITGSNGKSTVTDLTGVLAKAAGLNVGVGGNIGIPALDLLELDADLYVL
ELSSFQLETTSSLNLAAAAFLNLSEDHMDRYQGMADYRDAKLRIFNNAQYAIVNREDKETYPDHSMPLVTFGLDDQEFGV
ATIDGTEWLTDNGKPVLPSQDLTLVGRHNVANALVSLALLKQVGIDYNKSLEALKAYNGLTHRCQVVANKREIKWVNDSK
ATNVASTLAALSGLEYQGTLYLLVGGVGKGADFSELKPVLAQLDRVQLCCFGEDAAQFMPLHPSAKTFDTMRDIIESISA
QLVSGDMVMLSPACASFDQFNNFMARGDAFTELAHEYA

Sequences:

>Translated_438_residues
MERWQNIQNVVVVGLGITGLSVVKHLVKYQPQTHVRVIDTRELPPGKESLPESVELHSGSWNSQWLAEADLVVANPGIAL
ATSEIQDVIQAGTPVVGDIELFGWAVNKPAVAITGSNGKSTVTDLTGVLAKAAGLNVGVGGNIGIPALDLLELDADLYVL
ELSSFQLETTSSLNLAAAAFLNLSEDHMDRYQGMADYRDAKLRIFNNAQYAIVNREDKETYPDHSMPLVTFGLDDQEFGV
ATIDGTEWLTDNGKPVLPSQDLTLVGRHNVANALVSLALLKQVGIDYNKSLEALKAYNGLTHRCQVVANKREIKWVNDSK
ATNVASTLAALSGLEYQGTLYLLVGGVGKGADFSELKPVLAQLDRVQLCCFGEDAAQFMPLHPSAKTFDTMRDIIESISA
QLVSGDMVMLSPACASFDQFNNFMARGDAFTELAHEYA
>Mature_438_residues
MERWQNIQNVVVVGLGITGLSVVKHLVKYQPQTHVRVIDTRELPPGKESLPESVELHSGSWNSQWLAEADLVVANPGIAL
ATSEIQDVIQAGTPVVGDIELFGWAVNKPAVAITGSNGKSTVTDLTGVLAKAAGLNVGVGGNIGIPALDLLELDADLYVL
ELSSFQLETTSSLNLAAAAFLNLSEDHMDRYQGMADYRDAKLRIFNNAQYAIVNREDKETYPDHSMPLVTFGLDDQEFGV
ATIDGTEWLTDNGKPVLPSQDLTLVGRHNVANALVSLALLKQVGIDYNKSLEALKAYNGLTHRCQVVANKREIKWVNDSK
ATNVASTLAALSGLEYQGTLYLLVGGVGKGADFSELKPVLAQLDRVQLCCFGEDAAQFMPLHPSAKTFDTMRDIIESISA
QLVSGDMVMLSPACASFDQFNNFMARGDAFTELAHEYA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=433, Percent_Identity=51.9630484988453, Blast_Score=398, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_VIBSL (B7VJ01)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416105.1
- ProteinModelPortal:   B7VJ01
- GeneID:   7160083
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0448
- HOGENOM:   HBG750024
- OMA:   FQVGRHR
- ProtClustDB:   PRK04663
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 47336; Mature: 47336

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERWQNIQNVVVVGLGITGLSVVKHLVKYQPQTHVRVIDTRELPPGKESLPESVELHSGS
CCHHHCCCEEEEEEECCHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHCCCCEEECCCC
WNSQWLAEADLVVANPGIALATSEIQDVIQAGTPVVGDIELFGWAVNKPAVAITGSNGKS
CCCCEEEECCEEEECCCCEEEHHHHHHHHHCCCCCEECCEEEEEEECCCEEEEECCCCCC
TVTDLTGVLAKAAGLNVGVGGNIGIPALDLLELDADLYVLELSSFQLETTSSLNLAAAAF
HHHHHHHHHHHHCCCEECCCCCCCCCCCEEEECCCCEEEEEECCEEEECCCCCHHHHHHH
LNLSEDHMDRYQGMADYRDAKLRIFNNAQYAIVNREDKETYPDHSMPLVTFGLDDQEFGV
HCCCHHHHHHHCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCEEEECCCCCCCCE
ATIDGTEWLTDNGKPVLPSQDLTLVGRHNVANALVSLALLKQVGIDYNKSLEALKAYNGL
EEECCCEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
THRCQVVANKREIKWVNDSKATNVASTLAALSGLEYQGTLYLLVGGVGKGADFSELKPVL
CCEEEEEECCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHH
AQLDRVQLCCFGEDAAQFMPLHPSAKTFDTMRDIIESISAQLVSGDMVMLSPACASFDQF
HHCCCEEEEEECCCHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHH
NNFMARGDAFTELAHEYA
HHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MERWQNIQNVVVVGLGITGLSVVKHLVKYQPQTHVRVIDTRELPPGKESLPESVELHSGS
CCHHHCCCEEEEEEECCHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHCCCCEEECCCC
WNSQWLAEADLVVANPGIALATSEIQDVIQAGTPVVGDIELFGWAVNKPAVAITGSNGKS
CCCCEEEECCEEEECCCCEEEHHHHHHHHHCCCCCEECCEEEEEEECCCEEEEECCCCCC
TVTDLTGVLAKAAGLNVGVGGNIGIPALDLLELDADLYVLELSSFQLETTSSLNLAAAAF
HHHHHHHHHHHHCCCEECCCCCCCCCCCEEEECCCCEEEEEECCEEEECCCCCHHHHHHH
LNLSEDHMDRYQGMADYRDAKLRIFNNAQYAIVNREDKETYPDHSMPLVTFGLDDQEFGV
HCCCHHHHHHHCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCEEEECCCCCCCCE
ATIDGTEWLTDNGKPVLPSQDLTLVGRHNVANALVSLALLKQVGIDYNKSLEALKAYNGL
EEECCCEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
THRCQVVANKREIKWVNDSKATNVASTLAALSGLEYQGTLYLLVGGVGKGADFSELKPVL
CCEEEEEECCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHH
AQLDRVQLCCFGEDAAQFMPLHPSAKTFDTMRDIIESISAQLVSGDMVMLSPACASFDQF
HHCCCEEEEEECCCHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHH
NNFMARGDAFTELAHEYA
HHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA