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The map label for this gene is parE [H]

Identifier: 218708460

GI number: 218708460

Start: 432188

End: 434068

Strand: Direct

Name: parE [H]

Synonym: VS_0423

Alternate gene names: 218708460

Gene position: 432188-434068 (Clockwise)

Preceding gene: 218708459

Following gene: 218708461

Centisome position: 13.1

GC content: 47.95

Gene sequence:

>1881_bases
ATGACTGAACAATATAATGCAAAAGACCTCGAGGTACTTGAAGGTCTCGACCCCGTGCGACATCGCCCGGGAATGTATAC
CGAGACAGAAAGACCAAACCACCTTGCCCAAGAAGTCATTGATAACTCGGTTGATGAAGCACTAGCGGGACACGCTAAGA
AGATTAAAGTCGTGTTGCATGCTGACCAATCGTTAGAAGTGACGGATGATGGCCGTGGTATGCCGGTTGATATCCACCCT
GAAAAAGGGATCTCAGGTGTTGAGCTGATTTTAACTAAGCTCCACTCTGGCGGTAAGTTCTCGAACAATAACTACAAGTT
TTCAGGTGGTTTGCACGGGGTAGGTATCTCGGTGGTAAACGCGTTGTCAAAACGTGTTGAAGTGACGGTTCGCCGTGACG
GTCAGGTACATGAAATTGCCCTTGAAGGTGGTCATGCCGTAACGGATCTTACTGTTACAGGTACTTGTGGTCATCGCAAT
ACAGGTACGACGGTACATTTTTGGCCAGATCCAAAATATTTCGATAGCTCTAAATTTTCGACACTTCGTCTAATTAACAA
CCTACGTGCAAAAGCAGTTCTTTGCCCAGGTTTAGAAATTACCTTTGTCGACAAGGTTGGCGGTGAAGAACATAAATGGT
TCTATGAAGATGGCCTAAAAGACTACCTTGCCGAAGGCGTGAAAGGTTACACCTTACTTCCTGAAGAACCGTATGTTGGC
GAATTCGTAGCTGAAACAGAAATGGCAAACTGGGCGATCATTTGGCAGCCAGAAGGCGGTGATATGATCACCGAAAGTTA
CGTGAACTTAGTACCGACTAAACAAGGTGGTACACACGTAAACGGTCTTCGCCAAGGTCTGCTTGATGCAATGCGTGAGT
TCTGTGAATTCCGCAACTTGCTACCACGTGGGGTTAAGCTAACGGGCGACGATATTTTCGATCGCTGTTCGTACGTACTC
TCGGTGAAGATGCAAGATCCGCAGTTTGCTGGTCAAACAAAAGAGCGCTTGTCTTCTCGTCAAACCGCTGCGTTTGTTTC
TGGTGTGGTAAAAGATGCGTTTAGCTTGTGGTTGAACGAAAAGCCACAATTGGCAGAACAGTTAGCAGAAGCTTGTATTG
CGAATGCTCACCGCCGTATGCGCGCAAGCAAAAAGGTTGTGCGTAAGAAAATCGCGTCAGGCCCAGCACTGCCGGGTAAG
CTAACCGACTGTTCGGTTCAAGACTTAAGCCGTACCGAAATCTTCTTCGTGGAAGGGGACTCGGCGGGTGGTTCTGCTAA
ACAAGCTCGTGATCGTGAGTTCCAAGCGGTGATGCCACTTCGAGGTAAGATCCTAAATACATGGGAAGTGTCAGCAGACC
AAGTATTGGCTTCGCAAGAAGTGCACGACATTTCGGTGGCTCTGGGTATCGACCCGGACAACGATGATTTATCAGGCCTG
CGTTACGGTAAGATCTGTATCCTTGCCGATGCGGACTCGGATGGTCTTCATATCGCGACACTGCTATGTGCATTGTTTAC
CCGCCATTTCCATGCATTGGTTGAAGCGGGTCATATCTATGTGGCAATGCCTCCTCTGTATCGTATCGATTGCGGTAAAG
AAGTGTTCTACGCACTCGATGACGCAGAGAAAGACGGTGTGCTTGAGCGACTATCGCAGAAGAAAGCCAAGATCAACGTG
CAACGATTCAAAGGTCTGGGTGAAATGAACCCACTTCAGTTGCGTGAAACCACCATGGATCCAAACACCCGTCGCCTTGT
TCAATTAACGATTGATGACAACGAAGCGACCAACGAGATGATGGACATGCTGCTTGGTAAAAAACGTGCAGATGATCGCC
GTACATGGCTACAGACTAACGGTGATATGGCCGAGGTATAA

Upstream 100 bases:

>100_bases
TCGCTCGTTCGCTCTACTGTGACGGTTCATAGTAATGATTTCGCTTTAGTCAAACTAGAGCCAGCGAACTGACGCAAACT
TTTTGAGTAAACTCCGTATT

Downstream 100 bases:

>100_bases
TGGATGTCTAACGAAATTACATATGATGGCGTTGAACAGCTGCCAATGCGCAAGTTCACCGAAGATGCCTACTTAAATTA
CTCAATGTACGTGATCATGG

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 626; Mature: 625

Protein sequence:

>626_residues
MTEQYNAKDLEVLEGLDPVRHRPGMYTETERPNHLAQEVIDNSVDEALAGHAKKIKVVLHADQSLEVTDDGRGMPVDIHP
EKGISGVELILTKLHSGGKFSNNNYKFSGGLHGVGISVVNALSKRVEVTVRRDGQVHEIALEGGHAVTDLTVTGTCGHRN
TGTTVHFWPDPKYFDSSKFSTLRLINNLRAKAVLCPGLEITFVDKVGGEEHKWFYEDGLKDYLAEGVKGYTLLPEEPYVG
EFVAETEMANWAIIWQPEGGDMITESYVNLVPTKQGGTHVNGLRQGLLDAMREFCEFRNLLPRGVKLTGDDIFDRCSYVL
SVKMQDPQFAGQTKERLSSRQTAAFVSGVVKDAFSLWLNEKPQLAEQLAEACIANAHRRMRASKKVVRKKIASGPALPGK
LTDCSVQDLSRTEIFFVEGDSAGGSAKQARDREFQAVMPLRGKILNTWEVSADQVLASQEVHDISVALGIDPDNDDLSGL
RYGKICILADADSDGLHIATLLCALFTRHFHALVEAGHIYVAMPPLYRIDCGKEVFYALDDAEKDGVLERLSQKKAKINV
QRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDNEATNEMMDMLLGKKRADDRRTWLQTNGDMAEV

Sequences:

>Translated_626_residues
MTEQYNAKDLEVLEGLDPVRHRPGMYTETERPNHLAQEVIDNSVDEALAGHAKKIKVVLHADQSLEVTDDGRGMPVDIHP
EKGISGVELILTKLHSGGKFSNNNYKFSGGLHGVGISVVNALSKRVEVTVRRDGQVHEIALEGGHAVTDLTVTGTCGHRN
TGTTVHFWPDPKYFDSSKFSTLRLINNLRAKAVLCPGLEITFVDKVGGEEHKWFYEDGLKDYLAEGVKGYTLLPEEPYVG
EFVAETEMANWAIIWQPEGGDMITESYVNLVPTKQGGTHVNGLRQGLLDAMREFCEFRNLLPRGVKLTGDDIFDRCSYVL
SVKMQDPQFAGQTKERLSSRQTAAFVSGVVKDAFSLWLNEKPQLAEQLAEACIANAHRRMRASKKVVRKKIASGPALPGK
LTDCSVQDLSRTEIFFVEGDSAGGSAKQARDREFQAVMPLRGKILNTWEVSADQVLASQEVHDISVALGIDPDNDDLSGL
RYGKICILADADSDGLHIATLLCALFTRHFHALVEAGHIYVAMPPLYRIDCGKEVFYALDDAEKDGVLERLSQKKAKINV
QRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDNEATNEMMDMLLGKKRADDRRTWLQTNGDMAEV
>Mature_625_residues
TEQYNAKDLEVLEGLDPVRHRPGMYTETERPNHLAQEVIDNSVDEALAGHAKKIKVVLHADQSLEVTDDGRGMPVDIHPE
KGISGVELILTKLHSGGKFSNNNYKFSGGLHGVGISVVNALSKRVEVTVRRDGQVHEIALEGGHAVTDLTVTGTCGHRNT
GTTVHFWPDPKYFDSSKFSTLRLINNLRAKAVLCPGLEITFVDKVGGEEHKWFYEDGLKDYLAEGVKGYTLLPEEPYVGE
FVAETEMANWAIIWQPEGGDMITESYVNLVPTKQGGTHVNGLRQGLLDAMREFCEFRNLLPRGVKLTGDDIFDRCSYVLS
VKMQDPQFAGQTKERLSSRQTAAFVSGVVKDAFSLWLNEKPQLAEQLAEACIANAHRRMRASKKVVRKKIASGPALPGKL
TDCSVQDLSRTEIFFVEGDSAGGSAKQARDREFQAVMPLRGKILNTWEVSADQVLASQEVHDISVALGIDPDNDDLSGLR
YGKICILADADSDGLHIATLLCALFTRHFHALVEAGHIYVAMPPLYRIDCGKEVFYALDDAEKDGVLERLSQKKAKINVQ
RFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDNEATNEMMDMLLGKKRADDRRTWLQTNGDMAEV

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=658, Percent_Identity=23.8601823708207, Blast_Score=123, Evalue=6e-28,
Organism=Homo sapiens, GI19913408, Length=630, Percent_Identity=23.4920634920635, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1789408, Length=628, Percent_Identity=75.6369426751592, Blast_Score=1019, Evalue=0.0,
Organism=Escherichia coli, GI48994957, Length=558, Percent_Identity=41.0394265232975, Blast_Score=389, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI212645845, Length=598, Percent_Identity=23.9130434782609, Blast_Score=115, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17535065, Length=628, Percent_Identity=21.4968152866242, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI212645657, Length=254, Percent_Identity=27.1653543307087, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=629, Percent_Identity=24.1653418124006, Blast_Score=137, Evalue=4e-33,
Organism=Drosophila melanogaster, GI17136538, Length=625, Percent_Identity=22.56, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 69462; Mature: 69331

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQYNAKDLEVLEGLDPVRHRPGMYTETERPNHLAQEVIDNSVDEALAGHAKKIKVVLH
CCCCCCCCHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHHCHHHHHHCCCCEEEEEEEE
ADQSLEVTDDGRGMPVDIHPEKGISGVELILTKLHSGGKFSNNNYKFSGGLHGVGISVVN
CCCCEEECCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHH
ALSKRVEVTVRRDGQVHEIALEGGHAVTDLTVTGTCGHRNTGTTVHFWPDPKYFDSSKFS
HHHHHEEEEEECCCCEEEEEEECCCEEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCHH
TLRLINNLRAKAVLCPGLEITFVDKVGGEEHKWFYEDGLKDYLAEGVKGYTLLPEEPYVG
HHHHHHHCCCCEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCC
EFVAETEMANWAIIWQPEGGDMITESYVNLVPTKQGGTHVNGLRQGLLDAMREFCEFRNL
HHHHHHCCCCEEEEEECCCCCCHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
LPRGVKLTGDDIFDRCSYVLSVKMQDPQFAGQTKERLSSRQTAAFVSGVVKDAFSLWLNE
CCCCCEECCHHHHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KPQLAEQLAEACIANAHRRMRASKKVVRKKIASGPALPGKLTDCSVQDLSRTEIFFVEGD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECC
SAGGSAKQARDREFQAVMPLRGKILNTWEVSADQVLASQEVHDISVALGIDPDNDDLSGL
CCCCCHHHHHCCCHHEECCCCCCEEEEEECCHHHHHHHCCCEEEEEEEECCCCCCCCCCE
RYGKICILADADSDGLHIATLLCALFTRHFHALVEAGHIYVAMPPLYRIDCGKEVFYALD
EECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCCCEEEEEEC
DAEKDGVLERLSQKKAKINVQRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDNEATNEM
CCCCCHHHHHHHHHHHHEEHHHHCCCCCCCCCEEEECCCCCCCEEEEEEEECCCHHHHHH
MDMLLGKKRADDRRTWLQTNGDMAEV
HHHHHCCCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
TEQYNAKDLEVLEGLDPVRHRPGMYTETERPNHLAQEVIDNSVDEALAGHAKKIKVVLH
CCCCCCCHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHHCHHHHHHCCCCEEEEEEEE
ADQSLEVTDDGRGMPVDIHPEKGISGVELILTKLHSGGKFSNNNYKFSGGLHGVGISVVN
CCCCEEECCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHH
ALSKRVEVTVRRDGQVHEIALEGGHAVTDLTVTGTCGHRNTGTTVHFWPDPKYFDSSKFS
HHHHHEEEEEECCCCEEEEEEECCCEEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCHH
TLRLINNLRAKAVLCPGLEITFVDKVGGEEHKWFYEDGLKDYLAEGVKGYTLLPEEPYVG
HHHHHHHCCCCEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCC
EFVAETEMANWAIIWQPEGGDMITESYVNLVPTKQGGTHVNGLRQGLLDAMREFCEFRNL
HHHHHHCCCCEEEEEECCCCCCHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
LPRGVKLTGDDIFDRCSYVLSVKMQDPQFAGQTKERLSSRQTAAFVSGVVKDAFSLWLNE
CCCCCEECCHHHHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KPQLAEQLAEACIANAHRRMRASKKVVRKKIASGPALPGKLTDCSVQDLSRTEIFFVEGD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECC
SAGGSAKQARDREFQAVMPLRGKILNTWEVSADQVLASQEVHDISVALGIDPDNDDLSGL
CCCCCHHHHHCCCHHEECCCCCCEEEEEECCHHHHHHHCCCEEEEEEEECCCCCCCCCCE
RYGKICILADADSDGLHIATLLCALFTRHFHALVEAGHIYVAMPPLYRIDCGKEVFYALD
EECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCCCEEEEEEC
DAEKDGVLERLSQKKAKINVQRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDNEATNEM
CCCCCHHHHHHHHHHHHEEHHHHCCCCCCCCCEEEECCCCCCCEEEEEEEECCCHHHHHH
MDMLLGKKRADDRRTWLQTNGDMAEV
HHHHHCCCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2170028; 8388096; 9278503; 8227000; 8980775 [H]