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The map label for this gene is cysI

Identifier: 218708353

GI number: 218708353

Start: 292105

End: 293832

Strand: Direct

Name: cysI

Synonym: VS_0300

Alternate gene names: 218708353

Gene position: 292105-293832 (Clockwise)

Preceding gene: 218708352

Following gene: 218708354

Centisome position: 8.85

GC content: 45.25

Gene sequence:

>1728_bases
ATGAGCAAGCAAGTAATAGAGCAAGAAGTGCTAGGTCAAGTACTGGGACCTTTGGCTGACAATGAACGTCTGAAGCGTGA
AAGTAAAAACCTTCGCGGTACGATTGAACAAGATCTTGAAGACCGTATCACTGGTGGTTTTACCGCAGATAACTTTCAAC
TGATCCGTTTCCACGGTATGTATCAACAAGACGACCGTGATATTCGTAATGAACGTACCAAGCAAAAGCTAGAACCTTTA
CATAACGTAATGCTTCGTGCGCGTATGCCTGGCGGCATCATCACTCCTAAGCAGTGGCTCGCGATTGATAAATTCGCAGA
TGAAAGCACCTCTTATGGTTCTATCCGTCTTACAACTCGTCAAACCTTCCAGTTCCATGGTGTGTTGAAGCCGAACATTA
AGTTAATGCACCAAACACTAAACAGTATTGGTATTGATTCCATTGCGACCGCTGGTGATGTAAACCGAAATGTTTTGTGT
ACCACAAACCCGGTTGAGTCCGAGCTACACCAAGAAGCTTATGAGTGGGCGAAAAAGATCAGTGAACACCTATTACCTAA
GACTCGTGCTTATGCTGAGATCTGGTTAGATGGTGAAAAGCTAGCAACAACGGATGAAGAACCTATCCTAGGTAGTAACT
ACTTACCGCGTAAGTTCAAGACGACGGTTGTAATTCCTCCGCAAAATGACGTAGATGTTCATGCTAACGATCTTAACTTT
ATCGCGATTGCTAAAGACGGAAAGCTGGTGGGCTTTAACGTATTAGTGGGCGGTGGTCTTGCAATGACGCACGGCGATAC
TTCTACTTATGCACGTAAAGCTGACGACTTTGGTTTTGTGCCATTAGAGAAAACGTTAGATGTAGCGGCAGCTGTTGTGA
CGACACAACGTGACTGGGGTAACCGTTCGAACCGTAAGAACGCAAAAACCAAATACACACTAGACCGTGTTGGTATTGAT
GTATTCAAAGCAGAAGTAGAAAAACGTGCAGGCGTTGAGTTTGCTGAAAGTCGTCCTTATGAGTTTACTGGCCGTGGCGA
CCGTATCGGTTGGGCGGAAGGTATTGATGGTAAGCACCACTTAGCGTTATTCATCGAAAATGGTCGTTTACTTGATTTTC
CGGGTAAAGCGCTGAAAACAGGTGTTGCTGAAATAGCGAAGATCCACAAAGGTGATTTCCGCATGACAGCGAACCAAAAT
CTCATTGTTGCAGGTGTACTTAAGAGCCAAAAGGCACAAATTGAAAAGCTGGCACGTCAATACGGTCTGATGGATGATGC
CGTTTCAGAACAGCGCAAGAACTCAATGGCGTGTGTGGCATTCCCAACATGTCCGTTAGCAATGGCAGAAGCCGAACGTT
TTCTTCCTGAGTTTGTAACGGATGTTGAAGACATTCTGAAGAAACACGGATTACCAGAAGAAGATAACATCATCCTTCGT
ATTACAGGCTGTCCAAACGGCTGTGGTCGTGCAATGTTGGCTGAACTTGGTTTAGTCGGCAAGGCTCCAGGGCGTTACAA
CATGCACTTAGGTGGCAACAAAGCCGGAACTCGTATTCCTAAGATGTATAAAGAGAACATCACGTCAGCTCAGATTTTAG
AAGAGATTGATTCGCTGGTGGGGCGTTGGGCTACGGAACGAACGGATAATGAAGGGTTCGGTGATTTTACAATCCGAGCT
GGCATCATCGAAGAGGTGATCATTTCAAAGAGGGATCTGCATGCATAA

Upstream 100 bases:

>100_bases
TTACGATAGCGGAAAAACATGGCAATCAGAGCCGAGAACAAGCTGAACAATATATTAATGATTTACGTAAAGCGAAACGT
TACCAAAGGGATGTGTACTA

Downstream 100 bases:

>100_bases
TTCTGTCGCTTCAAAACTGAAGTTAGCAGAGCTACTCGCATTGACTAAGACGGAGCAGATACTTCGTCTTGGACAAATTA
ATGCTGAGTTAGAACAGCTA

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP

Number of amino acids: Translated: 575; Mature: 574

Protein sequence:

>575_residues
MSKQVIEQEVLGQVLGPLADNERLKRESKNLRGTIEQDLEDRITGGFTADNFQLIRFHGMYQQDDRDIRNERTKQKLEPL
HNVMLRARMPGGIITPKQWLAIDKFADESTSYGSIRLTTRQTFQFHGVLKPNIKLMHQTLNSIGIDSIATAGDVNRNVLC
TTNPVESELHQEAYEWAKKISEHLLPKTRAYAEIWLDGEKLATTDEEPILGSNYLPRKFKTTVVIPPQNDVDVHANDLNF
IAIAKDGKLVGFNVLVGGGLAMTHGDTSTYARKADDFGFVPLEKTLDVAAAVVTTQRDWGNRSNRKNAKTKYTLDRVGID
VFKAEVEKRAGVEFAESRPYEFTGRGDRIGWAEGIDGKHHLALFIENGRLLDFPGKALKTGVAEIAKIHKGDFRMTANQN
LIVAGVLKSQKAQIEKLARQYGLMDDAVSEQRKNSMACVAFPTCPLAMAEAERFLPEFVTDVEDILKKHGLPEEDNIILR
ITGCPNGCGRAMLAELGLVGKAPGRYNMHLGGNKAGTRIPKMYKENITSAQILEEIDSLVGRWATERTDNEGFGDFTIRA
GIIEEVIISKRDLHA

Sequences:

>Translated_575_residues
MSKQVIEQEVLGQVLGPLADNERLKRESKNLRGTIEQDLEDRITGGFTADNFQLIRFHGMYQQDDRDIRNERTKQKLEPL
HNVMLRARMPGGIITPKQWLAIDKFADESTSYGSIRLTTRQTFQFHGVLKPNIKLMHQTLNSIGIDSIATAGDVNRNVLC
TTNPVESELHQEAYEWAKKISEHLLPKTRAYAEIWLDGEKLATTDEEPILGSNYLPRKFKTTVVIPPQNDVDVHANDLNF
IAIAKDGKLVGFNVLVGGGLAMTHGDTSTYARKADDFGFVPLEKTLDVAAAVVTTQRDWGNRSNRKNAKTKYTLDRVGID
VFKAEVEKRAGVEFAESRPYEFTGRGDRIGWAEGIDGKHHLALFIENGRLLDFPGKALKTGVAEIAKIHKGDFRMTANQN
LIVAGVLKSQKAQIEKLARQYGLMDDAVSEQRKNSMACVAFPTCPLAMAEAERFLPEFVTDVEDILKKHGLPEEDNIILR
ITGCPNGCGRAMLAELGLVGKAPGRYNMHLGGNKAGTRIPKMYKENITSAQILEEIDSLVGRWATERTDNEGFGDFTIRA
GIIEEVIISKRDLHA
>Mature_574_residues
SKQVIEQEVLGQVLGPLADNERLKRESKNLRGTIEQDLEDRITGGFTADNFQLIRFHGMYQQDDRDIRNERTKQKLEPLH
NVMLRARMPGGIITPKQWLAIDKFADESTSYGSIRLTTRQTFQFHGVLKPNIKLMHQTLNSIGIDSIATAGDVNRNVLCT
TNPVESELHQEAYEWAKKISEHLLPKTRAYAEIWLDGEKLATTDEEPILGSNYLPRKFKTTVVIPPQNDVDVHANDLNFI
AIAKDGKLVGFNVLVGGGLAMTHGDTSTYARKADDFGFVPLEKTLDVAAAVVTTQRDWGNRSNRKNAKTKYTLDRVGIDV
FKAEVEKRAGVEFAESRPYEFTGRGDRIGWAEGIDGKHHLALFIENGRLLDFPGKALKTGVAEIAKIHKGDFRMTANQNL
IVAGVLKSQKAQIEKLARQYGLMDDAVSEQRKNSMACVAFPTCPLAMAEAERFLPEFVTDVEDILKKHGLPEEDNIILRI
TGCPNGCGRAMLAELGLVGKAPGRYNMHLGGNKAGTRIPKMYKENITSAQILEEIDSLVGRWATERTDNEGFGDFTIRAG
IIEEVIISKRDLHA

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family

Homologues:

Organism=Escherichia coli, GI1789122, Length=560, Percent_Identity=71.7857142857143, Blast_Score=870, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322597, Length=578, Percent_Identity=44.8096885813149, Blast_Score=507, Evalue=1e-144,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): CYSI_VIBSL (B7VI85)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002415974.1
- GeneID:   7159951
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0300
- HOGENOM:   HBG626671
- OMA:   VGDEHIV
- ProtClustDB:   PRK13504
- HAMAP:   MF_01540
- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- PRINTS:   PR00397
- TIGRFAMs:   TIGR02041

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; SSF55124 NiR_SiRalpha_1/3

EC number: =1.8.1.2

Molecular weight: Translated: 64123; Mature: 63992

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQVIEQEVLGQVLGPLADNERLKRESKNLRGTIEQDLEDRITGGFTADNFQLIRFHGM
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEEECC
YQQDDRDIRNERTKQKLEPLHNVMLRARMPGGIITPKQWLAIDKFADESTSYGSIRLTTR
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEE
QTFQFHGVLKPNIKLMHQTLNSIGIDSIATAGDVNRNVLCTTNPVESELHQEAYEWAKKI
EEEEEECCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHH
SEHLLPKTRAYAEIWLDGEKLATTDEEPILGSNYLPRKFKTTVVIPPQNDVDVHANDLNF
HHHHCCCHHCEEEEEECCCEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCEE
IAIAKDGKLVGFNVLVGGGLAMTHGDTSTYARKADDFGFVPLEKTLDVAAAVVTTQRDWG
EEEECCCCEEEEEEEEECCEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHEECCCCC
NRSNRKNAKTKYTLDRVGIDVFKAEVEKRAGVEFAESRPYEFTGRGDRIGWAEGIDGKHH
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHCCCEEECCCCCCCCCCCCCCCCCE
LALFIENGRLLDFPGKALKTGVAEIAKIHKGDFRMTANQNLIVAGVLKSQKAQIEKLARQ
EEEEEECCEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHH
YGLMDDAVSEQRKNSMACVAFPTCPLAMAEAERFLPEFVTDVEDILKKHGLPEEDNIILR
HCCCHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
ITGCPNGCGRAMLAELGLVGKAPGRYNMHLGGNKAGTRIPKMYKENITSAQILEEIDSLV
EECCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHH
GRWATERTDNEGFGDFTIRAGIIEEVIISKRDLHA
HHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKQVIEQEVLGQVLGPLADNERLKRESKNLRGTIEQDLEDRITGGFTADNFQLIRFHGM
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEEECC
YQQDDRDIRNERTKQKLEPLHNVMLRARMPGGIITPKQWLAIDKFADESTSYGSIRLTTR
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEE
QTFQFHGVLKPNIKLMHQTLNSIGIDSIATAGDVNRNVLCTTNPVESELHQEAYEWAKKI
EEEEEECCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHH
SEHLLPKTRAYAEIWLDGEKLATTDEEPILGSNYLPRKFKTTVVIPPQNDVDVHANDLNF
HHHHCCCHHCEEEEEECCCEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCEE
IAIAKDGKLVGFNVLVGGGLAMTHGDTSTYARKADDFGFVPLEKTLDVAAAVVTTQRDWG
EEEECCCCEEEEEEEEECCEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHEECCCCC
NRSNRKNAKTKYTLDRVGIDVFKAEVEKRAGVEFAESRPYEFTGRGDRIGWAEGIDGKHH
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHCCCEEECCCCCCCCCCCCCCCCCE
LALFIENGRLLDFPGKALKTGVAEIAKIHKGDFRMTANQNLIVAGVLKSQKAQIEKLARQ
EEEEEECCEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHH
YGLMDDAVSEQRKNSMACVAFPTCPLAMAEAERFLPEFVTDVEDILKKHGLPEEDNIILR
HCCCHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
ITGCPNGCGRAMLAELGLVGKAPGRYNMHLGGNKAGTRIPKMYKENITSAQILEEIDSLV
EECCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHH
GRWATERTDNEGFGDFTIRAGIIEEVIISKRDLHA
HHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA