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The map label for this gene is cysH

Identifier: 218708354

GI number: 218708354

Start: 293825

End: 294601

Strand: Direct

Name: cysH

Synonym: VS_0301

Alternate gene names: 218708354

Gene position: 293825-294601 (Clockwise)

Preceding gene: 218708353

Following gene: 218708356

Centisome position: 8.91

GC content: 42.86

Gene sequence:

>777_bases
ATGCATAATTCTGTCGCTTCAAAACTGAAGTTAGCAGAGCTACTCGCATTGACTAAGACGGAGCAGATACTTCGTCTTGG
ACAAATTAATGCTGAGTTAGAACAGCTAACCGCATTAGAAAGAGTGAAGTGGGCTCTAGAAAACTTAGAAGGAACACATG
TGGTGTCTTCTAGTTTCGGAATCCAAGCAGCATTGATGCTGCACTTAGTGACTCAAGCCAAATCCGATATTCCAGTTATT
CTGACAGACACCGGGTACCTATTCCCAGAAACGTATCGCTTTATAGATGAGTTAAGTCAGAAGTTGACTCTAAACCTTCA
AGTCTTTCGCGCACAACAGAGCTCTAATTGGCAAGAAGCGCAATATGGCAAACTTTGGGATCAAGGTATAGAAGGGATAG
AGAAGTACAACAAGCTTAATAAAGTTGAACCGATGAGAAGAGCGCTGGATGAACTAGAGGCTGGCACTTGGTTTTCAGGG
TTGCGAAGAGAGCAATCTCAATCGCGCGCAAACTTGCCTATCTTATCTATCCAAAATGGTGTGTTTAAGTTCTTGCCAGT
AATTGATTGGACAAATAAAGATGTTCATTATTACTTAGAAGAGCATGGTCTCAGTTACCACCCACTTCGCGAGCAGGGGT
ACCTTTCTGTTGGAGATACTCATACGACTAAGAAATGGGAACCGGGTATGACTGAAGAAGAAACCCGGTTCAATGGTCTA
AAACGAGAATGTGGTCTCCATGAAGACGATGGAGAGCAATATGGTTCTGGGATTTAG

Upstream 100 bases:

>100_bases
GGGCGTTGGGCTACGGAACGAACGGATAATGAAGGGTTCGGTGATTTTACAATCCGAGCTGGCATCATCGAAGAGGTGAT
CATTTCAAAGAGGGATCTGC

Downstream 100 bases:

>100_bases
ACTCATTGCCATTTAAAAAGCTGCCTCAAGGCAGCTTTTTTAGTTTCTAGGGGATGAAATTAAAGCGATTGTGGATAAGT
CTGTGGTTATTATGTAGGTA

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MHNSVASKLKLAELLALTKTEQILRLGQINAELEQLTALERVKWALENLEGTHVVSSSFGIQAALMLHLVTQAKSDIPVI
LTDTGYLFPETYRFIDELSQKLTLNLQVFRAQQSSNWQEAQYGKLWDQGIEGIEKYNKLNKVEPMRRALDELEAGTWFSG
LRREQSQSRANLPILSIQNGVFKFLPVIDWTNKDVHYYLEEHGLSYHPLREQGYLSVGDTHTTKKWEPGMTEEETRFNGL
KRECGLHEDDGEQYGSGI

Sequences:

>Translated_258_residues
MHNSVASKLKLAELLALTKTEQILRLGQINAELEQLTALERVKWALENLEGTHVVSSSFGIQAALMLHLVTQAKSDIPVI
LTDTGYLFPETYRFIDELSQKLTLNLQVFRAQQSSNWQEAQYGKLWDQGIEGIEKYNKLNKVEPMRRALDELEAGTWFSG
LRREQSQSRANLPILSIQNGVFKFLPVIDWTNKDVHYYLEEHGLSYHPLREQGYLSVGDTHTTKKWEPGMTEEETRFNGL
KRECGLHEDDGEQYGSGI
>Mature_258_residues
MHNSVASKLKLAELLALTKTEQILRLGQINAELEQLTALERVKWALENLEGTHVVSSSFGIQAALMLHLVTQAKSDIPVI
LTDTGYLFPETYRFIDELSQKLTLNLQVFRAQQSSNWQEAQYGKLWDQGIEGIEKYNKLNKVEPMRRALDELEAGTWFSG
LRREQSQSRANLPILSIQNGVFKFLPVIDWTNKDVHYYLEEHGLSYHPLREQGYLSVGDTHTTKKWEPGMTEEETRFNGL
KRECGLHEDDGEQYGSGI

Specific function: Reduction of activated sulfate into sulfite

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily

Homologues:

Organism=Escherichia coli, GI1789121, Length=242, Percent_Identity=71.4876033057851, Blast_Score=366, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6325425, Length=233, Percent_Identity=30.0429184549356, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSH_VIBSL (B7VI86)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002415975.1
- GeneID:   7159952
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0301
- HOGENOM:   HBG758022
- OMA:   TNFRPYE
- ProtClustDB:   PRK02090
- GO:   GO:0005737
- HAMAP:   MF_00063
- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR011800
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00434
- TIGRFAMs:   TIGR02057

Pfam domain/function: PF01507 PAPS_reduct

EC number: =1.8.4.8

Molecular weight: Translated: 29523; Mature: 29523

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNSVASKLKLAELLALTKTEQILRLGQINAELEQLTALERVKWALENLEGTHVVSSSFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
IQAALMLHLVTQAKSDIPVILTDTGYLFPETYRFIDELSQKLTLNLQVFRAQQSSNWQEA
HHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHH
QYGKLWDQGIEGIEKYNKLNKVEPMRRALDELEAGTWFSGLRREQSQSRANLPILSIQNG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCC
VFKFLPVIDWTNKDVHYYLEEHGLSYHPLREQGYLSVGDTHTTKKWEPGMTEEETRFNGL
HHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHH
KRECGLHEDDGEQYGSGI
HHHCCCCCCCHHHCCCCC
>Mature Secondary Structure
MHNSVASKLKLAELLALTKTEQILRLGQINAELEQLTALERVKWALENLEGTHVVSSSFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
IQAALMLHLVTQAKSDIPVILTDTGYLFPETYRFIDELSQKLTLNLQVFRAQQSSNWQEA
HHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHH
QYGKLWDQGIEGIEKYNKLNKVEPMRRALDELEAGTWFSGLRREQSQSRANLPILSIQNG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCC
VFKFLPVIDWTNKDVHYYLEEHGLSYHPLREQGYLSVGDTHTTKKWEPGMTEEETRFNGL
HHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHH
KRECGLHEDDGEQYGSGI
HHHCCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA