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The map label for this gene is cysJ [H]

Identifier: 218708352

GI number: 218708352

Start: 290219

End: 292105

Strand: Direct

Name: cysJ [H]

Synonym: VS_0299

Alternate gene names: 218708352

Gene position: 290219-292105 (Clockwise)

Preceding gene: 218708351

Following gene: 218708353

Centisome position: 8.8

GC content: 45.26

Gene sequence:

>1887_bases
ATGATTAGGGATGTCGTGACCATGTCTTTAAATAAGAAAGAGTCTTCACAAAATAATAATGCACAGTCTGGGGCTAATGA
GTTACCTGGGCTAGCAGCACCACTTAATGACCAACAATTGGGTCATCTTCAGCAAACTGTTTCTGAATTATCTTCTCAAC
AACTGGCATGGGTCAGTGGTTACCTTTGGGGTGTGAGCCAAACTCAGCCTGTGGGTGCCGCCGCGCCAATCGCTCAAGCA
GCTGCTGCAGTAGCCGCAAAACCTGCGGGTAAGCTCAGCATTATCTTCGCATCCCAAACCGGTAATGCGAAAGGTGTCGC
TGAATCGCTTGAAGCAGAAGCGAAAGCCTTAGGGATTGCTGTCGAGCTTTTCGATGCAAGTGATTATAAAGGTAAGAACC
TAGCCAAAGAGACACACGTCATTTTTGTGGCTTCAACCAATGGTGAGGGCGAAGCCCCTGATAACGCCATTGAGTTGCAT
GAATTCCTTCAATCGAAGAAAGCGCCAAAATTATCGAATCTACAATACGGTGTGATCGGTTTAGGTGACTCAAGCTATGA
GTTCTTCTGCCAAACGGCTAAAGACTTCGATAACTTCCTCGCTAAGCTTGGTGCTAAATCGTTTGTCGACCGTCTTGATT
GTGATGTTGATTATGAAGAGTCAGCAACGGCATGGCGCGCTAAAGCATTAGAGCAAGTAAAAGAGACGCTATCGACAGGC
ACTGAAGCCGATGTAGTTCAGTTGCCCGTCGGTCAAGCGGCTGCCGGTCATTCGCAATACACCAAACAAAATCCATACAC
CGCGACCCTATTAACGAGCCAAAAGATCACCGGTCGTGATTCGGGCAAAGATGTTCGTCATATCGAGATTGATCTTGATG
AGTCGGGTATTACTTACCAACCGGGTGATGCGCTAGGTGTATGGTTTGAAAACAGTTCAGAACTCGCAAATCAGATTCTT
TCTAAGGTTGGGCTGTCTGGTATCGAGAGTGTCGATGTCGATGGTGACAACTTGTCTGTGCATAGCGCACTCGTCAGCAA
ATTTGAGATTACATCTTCAAACCCTCAGCTAGTGACTAAGTTTGCTGAGCTTTCGGGCAGTAAGAAGTTAATTAAGCTGG
TGGAAGATAAAGACAAGCTTCGTGAATACGCGGGTAATACTCAAGTTGTCGATGTTTTAGCTGAGAAGAAGACCAAGCTA
TCGGCTGATGAATTGATTGGCCTACTACGTAAGTTAACTCCACGCCTCTATTCTATTGCGTCAAGCCAAGCAGAAGTAGA
TGAAGAAGTTCACTTGACGGTTGGTCTGGTTGAATACCAAAAGGGCGATGAATCTCGTCTGGGTGGCGCTTCAAGTTTCT
TAGCTCAACGCCTTGAAGAAGGTGGTGAAGTGAAGGTGTTTGTAGAGAACAACAATAACTTCAAACTGCCGCAAGACGAC
AACACACCAATCATCATGGTTGGCCCGGGTACAGGTATCGCGCCTTTCCGTAGTTTTGTTCAAGAGCGTGAAAACAATGA
CGCTGAAGGTAAAAGCTGGTTGTTCTTTGGTGACCGTACCTTTACTCAAGATTTCTTATATCAAGTTGAATGGCAGAGGT
ATCTTAAATCTGGTGCATTAACCAAGCTTGATGTTGCGTTTAGTCGTGATCAAAAAGAAAAGGTTTATGTTCAAGACCGA
TTAATTGAACAGGCAGAGCAGGTTTGGCAATGGCTTCAAGAGGGCGCGTACCTCTATGTATGTGGCGATGCAACTCGAAT
GGCAAAAGATGTTCATGAAGCGTTGGTTACGATAGCGGAAAAACATGGCAATCAGAGCCGAGAACAAGCTGAACAATATA
TTAATGATTTACGTAAAGCGAAACGTTACCAAAGGGATGTGTACTAA

Upstream 100 bases:

>100_bases
CAATTGACGTTTCAATCGGTTTTTACTTCCAAAAAGTTATAAAACATACTTTTCTATTCTTTCTTGTTATTAAAAGGAGT
GGTTAATCTATCGTCACGCA

Downstream 100 bases:

>100_bases
TGAGCAAGCAAGTAATAGAGCAAGAAGTGCTAGGTCAAGTACTGGGACCTTTGGCTGACAATGAACGTCTGAAGCGTGAA
AGTAAAAACCTTCGCGGTAC

Product: sulfite reductase [NADPH] flavoprotein subunit alpha

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 628; Mature: 628

Protein sequence:

>628_residues
MIRDVVTMSLNKKESSQNNNAQSGANELPGLAAPLNDQQLGHLQQTVSELSSQQLAWVSGYLWGVSQTQPVGAAAPIAQA
AAAVAAKPAGKLSIIFASQTGNAKGVAESLEAEAKALGIAVELFDASDYKGKNLAKETHVIFVASTNGEGEAPDNAIELH
EFLQSKKAPKLSNLQYGVIGLGDSSYEFFCQTAKDFDNFLAKLGAKSFVDRLDCDVDYEESATAWRAKALEQVKETLSTG
TEADVVQLPVGQAAAGHSQYTKQNPYTATLLTSQKITGRDSGKDVRHIEIDLDESGITYQPGDALGVWFENSSELANQIL
SKVGLSGIESVDVDGDNLSVHSALVSKFEITSSNPQLVTKFAELSGSKKLIKLVEDKDKLREYAGNTQVVDVLAEKKTKL
SADELIGLLRKLTPRLYSIASSQAEVDEEVHLTVGLVEYQKGDESRLGGASSFLAQRLEEGGEVKVFVENNNNFKLPQDD
NTPIIMVGPGTGIAPFRSFVQERENNDAEGKSWLFFGDRTFTQDFLYQVEWQRYLKSGALTKLDVAFSRDQKEKVYVQDR
LIEQAEQVWQWLQEGAYLYVCGDATRMAKDVHEALVTIAEKHGNQSREQAEQYINDLRKAKRYQRDVY

Sequences:

>Translated_628_residues
MIRDVVTMSLNKKESSQNNNAQSGANELPGLAAPLNDQQLGHLQQTVSELSSQQLAWVSGYLWGVSQTQPVGAAAPIAQA
AAAVAAKPAGKLSIIFASQTGNAKGVAESLEAEAKALGIAVELFDASDYKGKNLAKETHVIFVASTNGEGEAPDNAIELH
EFLQSKKAPKLSNLQYGVIGLGDSSYEFFCQTAKDFDNFLAKLGAKSFVDRLDCDVDYEESATAWRAKALEQVKETLSTG
TEADVVQLPVGQAAAGHSQYTKQNPYTATLLTSQKITGRDSGKDVRHIEIDLDESGITYQPGDALGVWFENSSELANQIL
SKVGLSGIESVDVDGDNLSVHSALVSKFEITSSNPQLVTKFAELSGSKKLIKLVEDKDKLREYAGNTQVVDVLAEKKTKL
SADELIGLLRKLTPRLYSIASSQAEVDEEVHLTVGLVEYQKGDESRLGGASSFLAQRLEEGGEVKVFVENNNNFKLPQDD
NTPIIMVGPGTGIAPFRSFVQERENNDAEGKSWLFFGDRTFTQDFLYQVEWQRYLKSGALTKLDVAFSRDQKEKVYVQDR
LIEQAEQVWQWLQEGAYLYVCGDATRMAKDVHEALVTIAEKHGNQSREQAEQYINDLRKAKRYQRDVY
>Mature_628_residues
MIRDVVTMSLNKKESSQNNNAQSGANELPGLAAPLNDQQLGHLQQTVSELSSQQLAWVSGYLWGVSQTQPVGAAAPIAQA
AAAVAAKPAGKLSIIFASQTGNAKGVAESLEAEAKALGIAVELFDASDYKGKNLAKETHVIFVASTNGEGEAPDNAIELH
EFLQSKKAPKLSNLQYGVIGLGDSSYEFFCQTAKDFDNFLAKLGAKSFVDRLDCDVDYEESATAWRAKALEQVKETLSTG
TEADVVQLPVGQAAAGHSQYTKQNPYTATLLTSQKITGRDSGKDVRHIEIDLDESGITYQPGDALGVWFENSSELANQIL
SKVGLSGIESVDVDGDNLSVHSALVSKFEITSSNPQLVTKFAELSGSKKLIKLVEDKDKLREYAGNTQVVDVLAEKKTKL
SADELIGLLRKLTPRLYSIASSQAEVDEEVHLTVGLVEYQKGDESRLGGASSFLAQRLEEGGEVKVFVENNNNFKLPQDD
NTPIIMVGPGTGIAPFRSFVQERENNDAEGKSWLFFGDRTFTQDFLYQVEWQRYLKSGALTKLDVAFSRDQKEKVYVQDR
LIEQAEQVWQWLQEGAYLYVCGDATRMAKDVHEALVTIAEKHGNQSREQAEQYINDLRKAKRYQRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI7657393, Length=604, Percent_Identity=31.2913907284768, Blast_Score=246, Evalue=7e-65,
Organism=Homo sapiens, GI127139033, Length=609, Percent_Identity=30.8702791461412, Blast_Score=242, Evalue=7e-64,
Organism=Homo sapiens, GI221316709, Length=604, Percent_Identity=31.2913907284768, Blast_Score=241, Evalue=2e-63,
Organism=Homo sapiens, GI221316705, Length=613, Percent_Identity=30.8319738988581, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI221316707, Length=529, Percent_Identity=32.3251417769376, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI24041029, Length=598, Percent_Identity=31.438127090301, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI10835173, Length=648, Percent_Identity=27.9320987654321, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI40254422, Length=647, Percent_Identity=27.5115919629057, Blast_Score=169, Evalue=1e-41,
Organism=Homo sapiens, GI169790956, Length=417, Percent_Identity=30.4556354916067, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI169790958, Length=417, Percent_Identity=30.4556354916067, Blast_Score=161, Evalue=2e-39,
Organism=Escherichia coli, GI1789123, Length=601, Percent_Identity=51.9134775374376, Blast_Score=625, Evalue=1e-180,
Organism=Escherichia coli, GI1789154, Length=125, Percent_Identity=32.8, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17554134, Length=590, Percent_Identity=30.8474576271186, Blast_Score=249, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI17566446, Length=589, Percent_Identity=26.9949066213922, Blast_Score=164, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI17531441, Length=415, Percent_Identity=25.5421686746988, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI32563863, Length=125, Percent_Identity=35.2, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6321832, Length=632, Percent_Identity=29.4303797468354, Blast_Score=213, Evalue=9e-56,
Organism=Saccharomyces cerevisiae, GI6321143, Length=385, Percent_Identity=30.6493506493506, Blast_Score=147, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6325305, Length=632, Percent_Identity=24.8417721518987, Blast_Score=140, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6322597, Length=152, Percent_Identity=30.2631578947368, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17137192, Length=600, Percent_Identity=31.1666666666667, Blast_Score=238, Evalue=8e-63,
Organism=Drosophila melanogaster, GI24582192, Length=513, Percent_Identity=31.5789473684211, Blast_Score=210, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24660907, Length=597, Percent_Identity=28.3082077051926, Blast_Score=189, Evalue=6e-48,
Organism=Drosophila melanogaster, GI24660903, Length=597, Percent_Identity=28.3082077051926, Blast_Score=189, Evalue=6e-48,
Organism=Drosophila melanogaster, GI78706872, Length=536, Percent_Identity=29.1044776119403, Blast_Score=175, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24583543, Length=536, Percent_Identity=29.1044776119403, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI78706876, Length=536, Percent_Identity=29.1044776119403, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24647438, Length=479, Percent_Identity=24.2171189979123, Blast_Score=91, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 68887; Mature: 68887

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRDVVTMSLNKKESSQNNNAQSGANELPGLAAPLNDQQLGHLQQTVSELSSQQLAWVSG
CCCCEEEEECCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
YLWGVSQTQPVGAAAPIAQAAAAVAAKPAGKLSIIFASQTGNAKGVAESLEAEAKALGIA
HHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHEEE
VELFDASDYKGKNLAKETHVIFVASTNGEGEAPDNAIELHEFLQSKKAPKLSNLQYGVIG
EEEECCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEE
LGDSSYEFFCQTAKDFDNFLAKLGAKSFVDRLDCDVDYEESATAWRAKALEQVKETLSTG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCC
TEADVVQLPVGQAAAGHSQYTKQNPYTATLLTSQKITGRDSGKDVRHIEIDLDESGITYQ
CCCCEEEECCCCCCCCCCHHCCCCCEEEEEEECCCCCCCCCCCCEEEEEEEECCCCCEEC
PGDALGVWFENSSELANQILSKVGLSGIESVDVDGDNLSVHSALVSKFEITSSNPQLVTK
CCCEEEEEECCHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHEECCCCHHHHHH
FAELSGSKKLIKLVEDKDKLREYAGNTQVVDVLAEKKTKLSADELIGLLRKLTPRLYSIA
HHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SSQAEVDEEVHLTVGLVEYQKGDESRLGGASSFLAQRLEEGGEVKVFVENNNNFKLPQDD
CCHHHCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCEECCCCC
NTPIIMVGPGTGIAPFRSFVQERENNDAEGKSWLFFGDRTFTQDFLYQVEWQRYLKSGAL
CCCEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCC
TKLDVAFSRDQKEKVYVQDRLIEQAEQVWQWLQEGAYLYVCGDATRMAKDVHEALVTIAE
EEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHH
KHGNQSREQAEQYINDLRKAKRYQRDVY
HCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIRDVVTMSLNKKESSQNNNAQSGANELPGLAAPLNDQQLGHLQQTVSELSSQQLAWVSG
CCCCEEEEECCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
YLWGVSQTQPVGAAAPIAQAAAAVAAKPAGKLSIIFASQTGNAKGVAESLEAEAKALGIA
HHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHEEE
VELFDASDYKGKNLAKETHVIFVASTNGEGEAPDNAIELHEFLQSKKAPKLSNLQYGVIG
EEEECCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEE
LGDSSYEFFCQTAKDFDNFLAKLGAKSFVDRLDCDVDYEESATAWRAKALEQVKETLSTG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCC
TEADVVQLPVGQAAAGHSQYTKQNPYTATLLTSQKITGRDSGKDVRHIEIDLDESGITYQ
CCCCEEEECCCCCCCCCCHHCCCCCEEEEEEECCCCCCCCCCCCEEEEEEEECCCCCEEC
PGDALGVWFENSSELANQILSKVGLSGIESVDVDGDNLSVHSALVSKFEITSSNPQLVTK
CCCEEEEEECCHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHEECCCCHHHHHH
FAELSGSKKLIKLVEDKDKLREYAGNTQVVDVLAEKKTKLSADELIGLLRKLTPRLYSIA
HHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SSQAEVDEEVHLTVGLVEYQKGDESRLGGASSFLAQRLEEGGEVKVFVENNNNFKLPQDD
CCHHHCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCEECCCCC
NTPIIMVGPGTGIAPFRSFVQERENNDAEGKSWLFFGDRTFTQDFLYQVEWQRYLKSGAL
CCCEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCC
TKLDVAFSRDQKEKVYVQDRLIEQAEQVWQWLQEGAYLYVCGDATRMAKDVHEALVTIAE
EEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHH
KHGNQSREQAEQYINDLRKAKRYQRDVY
HCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]