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The map label for this gene is yjeA

Identifier: 218708311

GI number: 218708311

Start: 245537

End: 246508

Strand: Direct

Name: yjeA

Synonym: VS_0249

Alternate gene names: 218708311

Gene position: 245537-246508 (Clockwise)

Preceding gene: 218708306

Following gene: 218708317

Centisome position: 7.44

GC content: 47.22

Gene sequence:

>972_bases
ATGCACTCTACATGGCAACCGGCCGCAACCATTAAGCAGTTAAAGCAACGTGCTGATATCCTTAATCAAATTCGTCAGTT
CTTTGTTGAGCGAAACGTGATGGAAGTGGATACGCCTGCGATGAGCCACGCCACGGTGACGGATGTGCATTTACATACCT
TCAAAACTGAATTCGTAGGGCCAGGTTATGCGCACGGCCAGCCACTATTCTTTATGACTAGCCCAGAGTTTCATATGAAA
CGCTTGTTGGCTGCCGGAAGCGGTTGTATTTACCAAATTTGTAAATCTTTTCGCAATGAAGAAAATGGCCGTTATCACAA
CCCTGAGTTCACTATGTTGGAGTGGTATCGCGTTGGTTTTGATCATCATGATCTGATGGATGAGATGGATCTGCTGTTAC
AGCAGGTACTTAAATCAGGCACAGCAGAGCGAATGACTTACCAACAAGCCTTTATTGATGTGTTAGGTGTTTGTCCGCTG
GAAGATTCGATGGATACGCTAAAGCAAGCAGCTGCGAAACTAGGGCTCAGCGACATAGCTGACCCCGAGCAAGATCGCGA
TACGTTATTGCAGCTTCTTTTCAGTATTGGGGTCGAGGCGAAAATAGGCCAGCAGGTGCCTGCGTTTGTGTATGACTTCC
CAGCATCGCAAGCAGCGTTAGCTAAGATCAATCCAAACGATTCACGAGTCGCCGATCGCTTTGAGGTGTATTTCAAAGGA
ATTGAGTTAGCAAACGGCTTCCACGAGTTAGATAAACCGCAAGAACAACTTAAGCGCTTTGAAGATGATAATACCAAACG
TATTGAGATGGGCTTATCACCTCAACCGATTGATCATCACTTGATTGAGGCGTTAAAAGCGGGCTTACCAGATTGTGCGG
GTGTTGCTTTGGGTATCGACCGTCTGATCATGCTGGCGCTGGGCTACGACCATATCGATGATGTGACGGCTTTCCCGTTC
CCGCGCTCTTAG

Upstream 100 bases:

>100_bases
AACAACATTTCGCTTTCAAGAATAGAAAGATAGAAGCTTACAAAAGCGATGAAAGGTGGTAATTTCCGTCGTCTAGAATT
CAGTAATATGGAACTCAATA

Downstream 100 bases:

>100_bases
CTCTCTATTCAGTTAGTCCTACTTACCGTTTGGCTAACTGACCAAAATACCAGTACCCACTACGGCAACAATGGCACCTA
TCCAAGCGTAGACGTTCGGT

Product: lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MHSTWQPAATIKQLKQRADILNQIRQFFVERNVMEVDTPAMSHATVTDVHLHTFKTEFVGPGYAHGQPLFFMTSPEFHMK
RLLAAGSGCIYQICKSFRNEENGRYHNPEFTMLEWYRVGFDHHDLMDEMDLLLQQVLKSGTAERMTYQQAFIDVLGVCPL
EDSMDTLKQAAAKLGLSDIADPEQDRDTLLQLLFSIGVEAKIGQQVPAFVYDFPASQAALAKINPNDSRVADRFEVYFKG
IELANGFHELDKPQEQLKRFEDDNTKRIEMGLSPQPIDHHLIEALKAGLPDCAGVALGIDRLIMLALGYDHIDDVTAFPF
PRS

Sequences:

>Translated_323_residues
MHSTWQPAATIKQLKQRADILNQIRQFFVERNVMEVDTPAMSHATVTDVHLHTFKTEFVGPGYAHGQPLFFMTSPEFHMK
RLLAAGSGCIYQICKSFRNEENGRYHNPEFTMLEWYRVGFDHHDLMDEMDLLLQQVLKSGTAERMTYQQAFIDVLGVCPL
EDSMDTLKQAAAKLGLSDIADPEQDRDTLLQLLFSIGVEAKIGQQVPAFVYDFPASQAALAKINPNDSRVADRFEVYFKG
IELANGFHELDKPQEQLKRFEDDNTKRIEMGLSPQPIDHHLIEALKAGLPDCAGVALGIDRLIMLALGYDHIDDVTAFPF
PRS
>Mature_323_residues
MHSTWQPAATIKQLKQRADILNQIRQFFVERNVMEVDTPAMSHATVTDVHLHTFKTEFVGPGYAHGQPLFFMTSPEFHMK
RLLAAGSGCIYQICKSFRNEENGRYHNPEFTMLEWYRVGFDHHDLMDEMDLLLQQVLKSGTAERMTYQQAFIDVLGVCPL
EDSMDTLKQAAAKLGLSDIADPEQDRDTLLQLLFSIGVEAKIGQQVPAFVYDFPASQAALAKINPNDSRVADRFEVYFKG
IELANGFHELDKPQEQLKRFEDDNTKRIEMGLSPQPIDHHLIEALKAGLPDCAGVALGIDRLIMLALGYDHIDDVTAFPF
PRS

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI194272210, Length=342, Percent_Identity=27.4853801169591, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI5031815, Length=342, Percent_Identity=27.4853801169591, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI87082379, Length=321, Percent_Identity=60.7476635514019, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1790571, Length=336, Percent_Identity=30.3571428571429, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1789256, Length=341, Percent_Identity=31.0850439882698, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71994340, Length=341, Percent_Identity=25.8064516129032, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17535927, Length=341, Percent_Identity=25.8064516129032, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17535925, Length=341, Percent_Identity=25.8064516129032, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320242, Length=344, Percent_Identity=26.453488372093, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324256, Length=345, Percent_Identity=29.2753623188406, Blast_Score=105, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24640851, Length=336, Percent_Identity=25.8928571428571, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24640849, Length=336, Percent_Identity=25.8928571428571, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YJEA_VIBSL (B7VHR8)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002415932.1
- GeneID:   7159909
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0249
- HOGENOM:   HBG631383
- OMA:   MPEASGI
- ProtClustDB:   PRK09350
- GO:   GO:0005737
- HAMAP:   MF_00174
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- PANTHER:   PTHR22594
- TIGRFAMs:   TIGR00462

Pfam domain/function: PF00152 tRNA-synt_2

EC number: 6.1.1.6

Molecular weight: Translated: 36556; Mature: 36556

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSTWQPAATIKQLKQRADILNQIRQFFVERNVMEVDTPAMSHATVTDVHLHTFKTEFVG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHCC
PGYAHGQPLFFMTSPEFHMKRLLAAGSGCIYQICKSFRNEENGRYHNPEFTMLEWYRVGF
CCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEECCCCHHHHHHHHCC
DHHDLMDEMDLLLQQVLKSGTAERMTYQQAFIDVLGVCPLEDSMDTLKQAAAKLGLSDIA
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCC
DPEQDRDTLLQLLFSIGVEAKIGQQVPAFVYDFPASQAALAKINPNDSRVADRFEVYFKG
CCCCHHHHHHHHHHHHCCCHHCCCCCCHHHEECCCCCCEEEEECCCHHHHHHHHHHHHHH
IELANGFHELDKPQEQLKRFEDDNTKRIEMGLSPQPIDHHLIEALKAGLPDCAGVALGID
HHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHH
RLIMLALGYDHIDDVTAFPFPRS
HHHHHHHCCCHHCCCCCCCCCCC
>Mature Secondary Structure
MHSTWQPAATIKQLKQRADILNQIRQFFVERNVMEVDTPAMSHATVTDVHLHTFKTEFVG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHCC
PGYAHGQPLFFMTSPEFHMKRLLAAGSGCIYQICKSFRNEENGRYHNPEFTMLEWYRVGF
CCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEECCCCHHHHHHHHCC
DHHDLMDEMDLLLQQVLKSGTAERMTYQQAFIDVLGVCPLEDSMDTLKQAAAKLGLSDIA
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCC
DPEQDRDTLLQLLFSIGVEAKIGQQVPAFVYDFPASQAALAKINPNDSRVADRFEVYFKG
CCCCHHHHHHHHHHHHCCCHHCCCCCCHHHEECCCCCCEEEEECCCHHHHHHHHHHHHHH
IELANGFHELDKPQEQLKRFEDDNTKRIEMGLSPQPIDHHLIEALKAGLPDCAGVALGID
HHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHH
RLIMLALGYDHIDDVTAFPFPRS
HHHHHHHCCCHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA