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The map label for this gene is spsK [H]

Identifier: 218708292

GI number: 218708292

Start: 224208

End: 225068

Strand: Direct

Name: spsK [H]

Synonym: VS_0230

Alternate gene names: 218708292

Gene position: 224208-225068 (Clockwise)

Preceding gene: 218708291

Following gene: 218708293

Centisome position: 6.8

GC content: 39.14

Gene sequence:

>861_bases
ATGAAAGTTGTTATTACAGGAAAAGGCGGTCAGCTTGCTTACGAGTTAGAGCAACTTGCTCCACAAGGCGTTGAAGTTAT
TAGTGTTGGAATTAATGAATTTGATATTACCAATGAGATATTGGTTACGGAGTTTCTAATCAACACTAAACCTGACTTAG
TCATTAATGCTGCTGCTTATACAGCTGTAGATAAGGCAGAAGAAGATATAGATGCTGCTTATGCCGTGAATGAGCTAGGT
ACTAAATACTTAGCTAACGCGTGTAAGCAAATCAACGCACGTATTTTACATGTGTCGACTGATTTTGTATTTGATGGTAC
TAGTAGTGCTCCTTATCAAACTAGTTCTGAGCCTAACCCTATTAATGTTTATGGTGCTTCAAAACTTGCTGGAGAATTAG
TCTTACAAGAAATATTGCCTGAAGCTAGTGTGATTGTACGCTCTGCTTGGGTGTATTCTATTAACGGCAATAATTTCGTT
AAAAGTATGCTGTGCTTGATGCAAGAAAAACAACAACTTGGCATTATTTATGATCAAGTGGGCACACCAACTTGGGCTAA
AGGCCTCGCGCAATGGCTATGGACTATAGCTGATAGACCTGAAGTGACAGGGATTCACCATTGGACTGATGCTGGTGTTG
CTTCTTGGTATGACTTTGCGATCGCTATTCAAGAGTTAGGTGTTGAGAAAGGGCTATTAAAAGAGGCAATCCCTATTTTT
CCTATCTCAACTTCAGAATACCCAACTTTGGCTAAACGTCCCGAATTTTCAGTGATTGATAAATTTAGTGCAGAAGAAGT
CTCTGGTATTAAAACAACACACTGGCGAAAGCAGTTATCAGAAATGTTGGACAGTTTGTAA

Upstream 100 bases:

>100_bases
GGCCATTAGTTGATGGTGAACTACCTCAATTATCTGCAAAAGACGCAGATGCTTTCTCATTTAAAGATGCTCCAAAATTT
GATTAATGGGTAAATCTCAT

Downstream 100 bases:

>100_bases
TTATATAGTTGCTGTATATTCTAAAGCATGAGATAGAAATACTATTCTAGTATAGGAGGGTATGTGAAGTATAGTAAACC
ACAAGTTGCGGTGTTGTTAG

Product: putative dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKVVITGKGGQLAYELEQLAPQGVEVISVGINEFDITNEILVTEFLINTKPDLVINAAAYTAVDKAEEDIDAAYAVNELG
TKYLANACKQINARILHVSTDFVFDGTSSAPYQTSSEPNPINVYGASKLAGELVLQEILPEASVIVRSAWVYSINGNNFV
KSMLCLMQEKQQLGIIYDQVGTPTWAKGLAQWLWTIADRPEVTGIHHWTDAGVASWYDFAIAIQELGVEKGLLKEAIPIF
PISTSEYPTLAKRPEFSVIDKFSAEEVSGIKTTHWRKQLSEMLDSL

Sequences:

>Translated_286_residues
MKVVITGKGGQLAYELEQLAPQGVEVISVGINEFDITNEILVTEFLINTKPDLVINAAAYTAVDKAEEDIDAAYAVNELG
TKYLANACKQINARILHVSTDFVFDGTSSAPYQTSSEPNPINVYGASKLAGELVLQEILPEASVIVRSAWVYSINGNNFV
KSMLCLMQEKQQLGIIYDQVGTPTWAKGLAQWLWTIADRPEVTGIHHWTDAGVASWYDFAIAIQELGVEKGLLKEAIPIF
PISTSEYPTLAKRPEFSVIDKFSAEEVSGIKTTHWRKQLSEMLDSL
>Mature_286_residues
MKVVITGKGGQLAYELEQLAPQGVEVISVGINEFDITNEILVTEFLINTKPDLVINAAAYTAVDKAEEDIDAAYAVNELG
TKYLANACKQINARILHVSTDFVFDGTSSAPYQTSSEPNPINVYGASKLAGELVLQEILPEASVIVRSAWVYSINGNNFV
KSMLCLMQEKQQLGIIYDQVGTPTWAKGLAQWLWTIADRPEVTGIHHWTDAGVASWYDFAIAIQELGVEKGLLKEAIPIF
PISTSEYPTLAKRPEFSVIDKFSAEEVSGIKTTHWRKQLSEMLDSL

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=302, Percent_Identity=25.1655629139073, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI11034825, Length=302, Percent_Identity=25.1655629139073, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788352, Length=295, Percent_Identity=34.5762711864407, Blast_Score=181, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 31485; Mature: 31485

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVITGKGGQLAYELEQLAPQGVEVISVGINEFDITNEILVTEFLINTKPDLVINAAAY
CEEEEECCCCCHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEHHHH
TAVDKAEEDIDAAYAVNELGTKYLANACKQINARILHVSTDFVFDGTSSAPYQTSSEPNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECEEEECCCCCCCCCCCCCCC
INVYGASKLAGELVLQEILPEASVIVRSAWVYSINGNNFVKSMLCLMQEKQQLGIIYDQV
EEEEEHHHHHHHHHHHHHCCHHHHEEEEEEEEEECCCHHHHHHHHHHHHHHHCCEEEECC
GTPTWAKGLAQWLWTIADRPEVTGIHHWTDAGVASWYDFAIAIQELGVEKGLLKEAIPIF
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCEE
PISTSEYPTLAKRPEFSVIDKFSAEEVSGIKTTHWRKQLSEMLDSL
ECCCCCCCCCCCCCCCHHHHCCCHHHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVVITGKGGQLAYELEQLAPQGVEVISVGINEFDITNEILVTEFLINTKPDLVINAAAY
CEEEEECCCCCHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEHHHH
TAVDKAEEDIDAAYAVNELGTKYLANACKQINARILHVSTDFVFDGTSSAPYQTSSEPNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECEEEECCCCCCCCCCCCCCC
INVYGASKLAGELVLQEILPEASVIVRSAWVYSINGNNFVKSMLCLMQEKQQLGIIYDQV
EEEEEHHHHHHHHHHHHHCCHHHHEEEEEEEEEECCCHHHHHHHHHHHHHHHCCEEEECC
GTPTWAKGLAQWLWTIADRPEVTGIHHWTDAGVASWYDFAIAIQELGVEKGLLKEAIPIF
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCEE
PISTSEYPTLAKRPEFSVIDKFSAEEVSGIKTTHWRKQLSEMLDSL
ECCCCCCCCCCCCCCCHHHHCCCHHHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]