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The map label for this gene is pyrE [H]

Identifier: 218708241

GI number: 218708241

Start: 175378

End: 176019

Strand: Direct

Name: pyrE [H]

Synonym: VS_0178

Alternate gene names: 218708241

Gene position: 175378-176019 (Clockwise)

Preceding gene: 218708240

Following gene: 218708245

Centisome position: 5.32

GC content: 45.64

Gene sequence:

>642_bases
ATGAAAGCATATCAACGTGAATTTATTGAATTTGCACTAGAGAAAGAAGTACTTAAGTTTGGTGAGTTTACTTTAAAGTC
TGGCCGTAAGAGCCCTTACTTCTTCAATGCTGGATTGTTTAATACAGGTCGTGACCTAGCACGTTTAGGTCGCTTCTACG
CAGCAGCATTGGCAGATTCAGGTATTGAGTTCGATGTACTATTTGGCCCTGCATACAAAGGCATCCCAATCGCAACAACA
ACAGCGGTTGCACTAGCGGATCACCATGATGTGGACACGCCTTACTGCTTCAACCGTAAAGAAGCGAAAAACCACGGTGA
AGGTGGCAACCTAGTGGGTAGCGAACTTGAAGGTCGCATCATGTTAGTTGATGACGTGATCACTGCTGGTACTGCGATTC
GTGAGTCGATGGAAATCATCCAAGCAAACGGCGCTGATTTAGCGGGTGTGCTTGTTGCTATCGACCGTCAAGAGAAGGGC
AAAGGTGAGTTGTCTGCGATTCAAGAAGTAGAGCGTGACTTCGGTTGTGCGATTATCTCAATCGTTAGCCTGACTGATTT
AGTGACTTTTCTTGAAGAGAAAGGCACAGACGCAGCACATCTTGAGTCAGTAAAAGCGTACCGAGCTCAATACGGAATCT
AA

Upstream 100 bases:

>100_bases
TTTCGCAAGCTTGTTGCCTAGCGGAAAACACGACTACTTAGAAGATACTAGGAAGAGTTTAAGTTAATTAAGTTTTATTG
AATAAACGTAGAGGAAAAAC

Downstream 100 bases:

>100_bases
TTCGTAAACTGAAGAAGTTGAACCTAATGGTTTAGTTCCCTCTGTTTAGAGTTTAAAAAAAGAACAATGCCCGAGAGATG
AAACTCTTTCGGGCATTTTT

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKAYQREFIEFALEKEVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLARLGRFYAAALADSGIEFDVLFGPAYKGIPIATT
TAVALADHHDVDTPYCFNRKEAKNHGEGGNLVGSELEGRIMLVDDVITAGTAIRESMEIIQANGADLAGVLVAIDRQEKG
KGELSAIQEVERDFGCAIISIVSLTDLVTFLEEKGTDAAHLESVKAYRAQYGI

Sequences:

>Translated_213_residues
MKAYQREFIEFALEKEVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLARLGRFYAAALADSGIEFDVLFGPAYKGIPIATT
TAVALADHHDVDTPYCFNRKEAKNHGEGGNLVGSELEGRIMLVDDVITAGTAIRESMEIIQANGADLAGVLVAIDRQEKG
KGELSAIQEVERDFGCAIISIVSLTDLVTFLEEKGTDAAHLESVKAYRAQYGI
>Mature_213_residues
MKAYQREFIEFALEKEVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLARLGRFYAAALADSGIEFDVLFGPAYKGIPIATT
TAVALADHHDVDTPYCFNRKEAKNHGEGGNLVGSELEGRIMLVDDVITAGTAIRESMEIIQANGADLAGVLVAIDRQEKG
KGELSAIQEVERDFGCAIISIVSLTDLVTFLEEKGTDAAHLESVKAYRAQYGI

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=195, Percent_Identity=28.2051282051282, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1790073, Length=213, Percent_Identity=80.2816901408451, Blast_Score=365, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17508631, Length=163, Percent_Identity=28.2208588957055, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17554964, Length=195, Percent_Identity=25.6410256410256, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323530, Length=220, Percent_Identity=49.0909090909091, Blast_Score=203, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6323927, Length=221, Percent_Identity=45.2488687782805, Blast_Score=190, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 23220; Mature: 23220

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAYQREFIEFALEKEVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLARLGRFYAAALADS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCC
GIEFDVLFGPAYKGIPIATTTAVALADHHDVDTPYCFNRKEAKNHGEGGNLVGSELEGRI
CCEEEEEECCCCCCCCCHHHHHEEECCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCE
MLVDDVITAGTAIRESMEIIQANGADLAGVLVAIDRQEKGKGELSAIQEVERDFGCAIIS
EEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
IVSLTDLVTFLEEKGTDAAHLESVKAYRAQYGI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKAYQREFIEFALEKEVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLARLGRFYAAALADS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCC
GIEFDVLFGPAYKGIPIATTTAVALADHHDVDTPYCFNRKEAKNHGEGGNLVGSELEGRI
CCEEEEEECCCCCCCCCHHHHHEEECCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCE
MLVDDVITAGTAIRESMEIIQANGADLAGVLVAIDRQEKGKGELSAIQEVERDFGCAIIS
EEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
IVSLTDLVTFLEEKGTDAAHLESVKAYRAQYGI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]