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The map label for this gene is epsM [H]

Identifier: 218708208

GI number: 218708208

Start: 138689

End: 139171

Strand: Direct

Name: epsM [H]

Synonym: VS_0144

Alternate gene names: 218708208

Gene position: 138689-139171 (Clockwise)

Preceding gene: 218708207

Following gene: 218708209

Centisome position: 4.2

GC content: 43.69

Gene sequence:

>483_bases
ATGAGAAATATAATTGAACCACTCCAAGCGTGGTGGACTTCAATAAGTCAAAGAGAGCAACGATTAGTCGTTGGTTGCTC
AATTTTATTGGTAGTAGGTATCATTTATTGGGGGCTAATACAGCCCCTCAGTCAGCGAGCTGAACTGGCACAGAGTCGCA
TTCAAAGTGAAAAGCAACTACTGGCTTGGGTTACCAATAAAGCCAATGAGGTGGTTGAACTAAGAGGCAATGGTGGCATT
AGTGCTAGTCAGCCTCTGAATCAATCCGTGCCCGCATCTATGCGTCGCTTCAAGATTGAATTGATACGAGTGCAACCACG
TGGCGAGATGCTGCAAGTGTGGATTAAACCTTTGCCATTTAATCAATTTGTTGATTGGCTGACATACCTGAAAGAAAAGC
AGGGTGTTGAGGTTGAGTTTATGGATATTGATCGCTCTGATAGCCTTGGGGTTATTGACGTCAACCGACTACAGTTTAAA
TGA

Upstream 100 bases:

>100_bases
AAGTTTTCCGTTGAACAAGGGCCATTGAACCGTAATGGCGATGCCGTATTTGGCAGTTTTACCCTTAAACCCCGTCAGTA
ACTGCGCAAGGAGATCAGTG

Downstream 100 bases:

>100_bases
GGTTAATGTGAAACGCGGTTTATCTTTTAAGTATGGTTTGTTATTTAGCAGCATCTTTATCGTTTTTTTCTCGGTAAGTT
TGTTGCTGCACTTGCCGGCT

Product: general secretion pathway protein M

Products: NA

Alternate protein names: Cholera toxin secretion protein epsM [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MRNIIEPLQAWWTSISQREQRLVVGCSILLVVGIIYWGLIQPLSQRAELAQSRIQSEKQLLAWVTNKANEVVELRGNGGI
SASQPLNQSVPASMRRFKIELIRVQPRGEMLQVWIKPLPFNQFVDWLTYLKEKQGVEVEFMDIDRSDSLGVIDVNRLQFK

Sequences:

>Translated_160_residues
MRNIIEPLQAWWTSISQREQRLVVGCSILLVVGIIYWGLIQPLSQRAELAQSRIQSEKQLLAWVTNKANEVVELRGNGGI
SASQPLNQSVPASMRRFKIELIRVQPRGEMLQVWIKPLPFNQFVDWLTYLKEKQGVEVEFMDIDRSDSLGVIDVNRLQFK
>Mature_160_residues
MRNIIEPLQAWWTSISQREQRLVVGCSILLVVGIIYWGLIQPLSQRAELAQSRIQSEKQLLAWVTNKANEVVELRGNGGI
SASQPLNQSVPASMRRFKIELIRVQPRGEMLQVWIKPLPFNQFVDWLTYLKEKQGVEVEFMDIDRSDSLGVIDVNRLQFK

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG3149

COG function: function code U; Type II secretory pathway, component PulM

Gene ontology:

Cell location: Cell inner membrane (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP M family [H]

Homologues:

Organism=Escherichia coli, GI1789339, Length=145, Percent_Identity=28.9655172413793, Blast_Score=70, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007690
- InterPro:   IPR023229 [H]

Pfam domain/function: PF04612 GspM [H]

EC number: NA

Molecular weight: Translated: 18461; Mature: 18461

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNIIEPLQAWWTSISQREQRLVVGCSILLVVGIIYWGLIQPLSQRAELAQSRIQSEKQL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAWVTNKANEVVELRGNGGISASQPLNQSVPASMRRFKIELIRVQPRGEMLQVWIKPLPF
HHHHHCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHEEEEEEECCCCCEEHHHHCCCCH
NQFVDWLTYLKEKQGVEVEFMDIDRSDSLGVIDVNRLQFK
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHCEECC
>Mature Secondary Structure
MRNIIEPLQAWWTSISQREQRLVVGCSILLVVGIIYWGLIQPLSQRAELAQSRIQSEKQL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAWVTNKANEVVELRGNGGISASQPLNQSVPASMRRFKIELIRVQPRGEMLQVWIKPLPF
HHHHHCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHEEEEEEECCCCCEEHHHHCCCCH
NQFVDWLTYLKEKQGVEVEFMDIDRSDSLGVIDVNRLQFK
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8406031; 10952301 [H]