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The map label for this gene is epsF [H]

Identifier: 218708201

GI number: 218708201

Start: 132824

End: 134044

Strand: Direct

Name: epsF [H]

Synonym: VS_0137

Alternate gene names: 218708201

Gene position: 132824-134044 (Clockwise)

Preceding gene: 218708200

Following gene: 218708202

Centisome position: 4.03

GC content: 47.5

Gene sequence:

>1221_bases
ATGGCGGCATTTGAATACAAAGCGCTGGATGCCAAAGGTAAGAGCAAAAAGGGTTCGATTGAAGCGGATAATGCTCGTCA
GGCTCGCCAACGTTTAAAAGAACAAGGTTTAATGCCGGTTGAGATGACCGAAGCTAAGGCGAAAAATGCGAAAGGTACTC
AGCCTTCGACCAGCTTTAAGCGTGGTATCAGTACGCCAGATCTTGCGTTGATTACTCGTCAAATATCCACGCTCGTTCAA
TCAGGCATGCCGCTAGAAGAGTGCTTGAAAGCGGTCGCTGAGCAGTCTGAAAAGCCACGTATTCGAACTATGTTATTGGC
AGTCCGATCTAAAGTGACAGAAGGTTACTCGCTAGCGGATAGCTTGTCTGATTATCCACACATCTTCGATGAACTGTTTA
GGGCCATGGTTGCGGCGGGTGAGAAGTCTGGTCACTTAGATGCGGTATTGGAGCGACTGGCCGATTACGCAGAAAATCGT
CAGAAGATGCGCTCTAAGCTGCTACAAGCGATGATCTACCCTGTGGTGCTGGTGGTGTTTGCGGTGACGATTGTTTCGTT
CCTGTTGGCAACCGTGGTACCTAAGATCGTCGAGCCTATTATCCAAATGGGCCAAGAGCTTCCTCAGTCGACACAATTTT
TATTGGCATCGAGTGAATTTATCCAGAATTGGGGCATCCAATTACTGTTGTTGATCATTGGTGTGATTGTGTTGATTAAG
ACCGCGCTCAAAAAGCCGGGTGTTCGCATGAGCTGGGATCGCAAACTATTGAGTATTCCGCTGATCGGCAAGATTGCGAA
AGGGATCAATACCTCTCGTTTTGCACGAACACTCTCAATTTGTACCTCGAGTGCGATTCCAATCCTTGAAGGGATGAAGG
TCGCGGTTGATGTGATGTCGAATCAACATGTGAAACAACAAGTATTACAGGCTTCAGACAGTGTTAGAGAAGGCGCAAGC
CTACGTAAAGCGTTGGATCAAACCAAACTCTTTCCACCGATGATGCTGCATATGATTGCCAGTGGTGAGCAGAGTGGTCA
GTTGGAACAGATGCTGACAAGAGCGGCAGACAACCAAGACCAAAGCTTTGAGTCGACGGTCAATATCGCGTTAGGCATTT
TCACCCCAGCGCTTATCGCGTTGATGGCCGGTTTAGTGCTGTTTATCGTGATGGCGACACTGATGCCGATGCTTGAAATG
AACAATTTAATGAGTGGATAA

Upstream 100 bases:

>100_bases
CGTGGCACAACCCCAAGTATTCGAGATGATGGCTTGAGCAAAGTTCTGAAAGGGGTAACGTCCTTAGAAGAAGTGATGCG
AGTGACTAAGGAAGTCTAGT

Downstream 100 bases:

>100_bases
CGTACTGCTCATCAGACGTTAGTTTTTGGATTATCGAGAAGAAGGGCATTATTACCTTCAACTCGCTATCTGTAATTTGG
AGAAAATAATGAAAAATAAA

Product: type II secretory pathway subunit EpsF

Products: NA

Alternate protein names: Cholera toxin secretion protein epsF [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MAAFEYKALDAKGKSKKGSIEADNARQARQRLKEQGLMPVEMTEAKAKNAKGTQPSTSFKRGISTPDLALITRQISTLVQ
SGMPLEECLKAVAEQSEKPRIRTMLLAVRSKVTEGYSLADSLSDYPHIFDELFRAMVAAGEKSGHLDAVLERLADYAENR
QKMRSKLLQAMIYPVVLVVFAVTIVSFLLATVVPKIVEPIIQMGQELPQSTQFLLASSEFIQNWGIQLLLLIIGVIVLIK
TALKKPGVRMSWDRKLLSIPLIGKIAKGINTSRFARTLSICTSSAIPILEGMKVAVDVMSNQHVKQQVLQASDSVREGAS
LRKALDQTKLFPPMMLHMIASGEQSGQLEQMLTRAADNQDQSFESTVNIALGIFTPALIALMAGLVLFIVMATLMPMLEM
NNLMSG

Sequences:

>Translated_406_residues
MAAFEYKALDAKGKSKKGSIEADNARQARQRLKEQGLMPVEMTEAKAKNAKGTQPSTSFKRGISTPDLALITRQISTLVQ
SGMPLEECLKAVAEQSEKPRIRTMLLAVRSKVTEGYSLADSLSDYPHIFDELFRAMVAAGEKSGHLDAVLERLADYAENR
QKMRSKLLQAMIYPVVLVVFAVTIVSFLLATVVPKIVEPIIQMGQELPQSTQFLLASSEFIQNWGIQLLLLIIGVIVLIK
TALKKPGVRMSWDRKLLSIPLIGKIAKGINTSRFARTLSICTSSAIPILEGMKVAVDVMSNQHVKQQVLQASDSVREGAS
LRKALDQTKLFPPMMLHMIASGEQSGQLEQMLTRAADNQDQSFESTVNIALGIFTPALIALMAGLVLFIVMATLMPMLEM
NNLMSG
>Mature_405_residues
AAFEYKALDAKGKSKKGSIEADNARQARQRLKEQGLMPVEMTEAKAKNAKGTQPSTSFKRGISTPDLALITRQISTLVQS
GMPLEECLKAVAEQSEKPRIRTMLLAVRSKVTEGYSLADSLSDYPHIFDELFRAMVAAGEKSGHLDAVLERLADYAENRQ
KMRSKLLQAMIYPVVLVVFAVTIVSFLLATVVPKIVEPIIQMGQELPQSTQFLLASSEFIQNWGIQLLLLIIGVIVLIKT
ALKKPGVRMSWDRKLLSIPLIGKIAKGINTSRFARTLSICTSSAIPILEGMKVAVDVMSNQHVKQQVLQASDSVREGASL
RKALDQTKLFPPMMLHMIASGEQSGQLEQMLTRAADNQDQSFESTVNIALGIFTPALIALMAGLVLFIVMATLMPMLEMN
NLMSG

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=402, Percent_Identity=43.2835820895522, Blast_Score=342, Evalue=2e-95,
Organism=Escherichia coli, GI1786295, Length=405, Percent_Identity=22.962962962963, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 44546; Mature: 44415

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAFEYKALDAKGKSKKGSIEADNARQARQRLKEQGLMPVEMTEAKAKNAKGTQPSTSFK
CCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHH
RGISTPDLALITRQISTLVQSGMPLEECLKAVAEQSEKPRIRTMLLAVRSKVTEGYSLAD
HCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHH
SLSDYPHIFDELFRAMVAAGEKSGHLDAVLERLADYAENRQKMRSKLLQAMIYPVVLVVF
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVTIVSFLLATVVPKIVEPIIQMGQELPQSTQFLLASSEFIQNWGIQLLLLIIGVIVLIK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TALKKPGVRMSWDRKLLSIPLIGKIAKGINTSRFARTLSICTSSAIPILEGMKVAVDVMS
HHHHCCCCCCCCCHHHHHCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
NQHVKQQVLQASDSVREGASLRKALDQTKLFPPMMLHMIASGEQSGQLEQMLTRAADNQD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
QSFESTVNIALGIFTPALIALMAGLVLFIVMATLMPMLEMNNLMSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AAFEYKALDAKGKSKKGSIEADNARQARQRLKEQGLMPVEMTEAKAKNAKGTQPSTSFK
CCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHH
RGISTPDLALITRQISTLVQSGMPLEECLKAVAEQSEKPRIRTMLLAVRSKVTEGYSLAD
HCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHH
SLSDYPHIFDELFRAMVAAGEKSGHLDAVLERLADYAENRQKMRSKLLQAMIYPVVLVVF
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVTIVSFLLATVVPKIVEPIIQMGQELPQSTQFLLASSEFIQNWGIQLLLLIIGVIVLIK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TALKKPGVRMSWDRKLLSIPLIGKIAKGINTSRFARTLSICTSSAIPILEGMKVAVDVMS
HHHHCCCCCCCCCHHHHHCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
NQHVKQQVLQASDSVREGASLRKALDQTKLFPPMMLHMIASGEQSGQLEQMLTRAADNQD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
QSFESTVNIALGIFTPALIALMAGLVLFIVMATLMPMLEMNNLMSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]