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The map label for this gene is epsE [H]

Identifier: 218708200

GI number: 218708200

Start: 131320

End: 132822

Strand: Direct

Name: epsE [H]

Synonym: VS_0136

Alternate gene names: 218708200

Gene position: 131320-132822 (Clockwise)

Preceding gene: 218708199

Following gene: 218708201

Centisome position: 3.98

GC content: 47.7

Gene sequence:

>1503_bases
ATGGCTGAATTGGTAGGGGCGGCACGTACTTATCAGCGCTTGCCGTTTAGCTTTGCGAATCGCTACAAGATGGTGTTGGA
ATATCAACACCCGGAGCGCGCGCCGGTACTTTATTATGTTGAACCTCTGAAATCGGCGGCGATCATTGAAGTGAGCCGTG
TTGTGAAAAATGGCTTCACGCCACAAGCGATCAGCGCAGACGAATTTGATAAAAAACTGACGGATGCGTACCAGCGTGAC
TCGTCGGAAGCTCGTCAGTTGATGGAAGACATTGGTGCCGACAATGACGACTTCTTCTCTCTAGCAGAAGAGCTACCACA
AGATGAAGACTTGCTCGAATCTGAAGACGATGCGCCAATCATCAAGCTGATTAATGCGATGTTGGGTGAGGCGATCAAAG
AAGGTGCTTCGGATATTCATATCGAAACCTTTGAGAAATCACTGTCTATCCGTTTCCGTATTGATGGCGTGTTACGTGAT
GTTCTTGCGCCAAGCCGCAAATTGGCCCCGCTGTTGGTTTCGCGTGTCAAGGTTATGGCTAAGCTGGATATTGCGGAAAA
ACGCGTGCCACAAGATGGTCGTATTTCTCTGCGTATTGGTGGTCGAGCGGTCGACGTGCGTGTGTCTACCATGCCCTCTT
CACATGGTGAACGTGTAGTAATGCGTCTGTTGGACAAAAACGCCACTCGCCTAGATCTACACAGTTTGGGTATGACGGCA
GAGAACCACGAGAACTTCCGTAAACTTATTCAGCGCCCACACGGTATTATCTTGGTTACTGGTCCTACGGGTTCTGGTAA
ATCAACGACCTTGTACGCTGGCCTGCAAGAACTCAACAGTAACGAACGCAACATCCTTACCGTTGAAGATCCAATCGAAT
TTGATATTGATGGTATTGGTCAAACACAGGTAAACCCTAAGGTTGATATGACCTTTGCGCGCGGCTTACGTGCGATTCTT
CGTCAAGATCCCGATGTGGTAATGATTGGTGAGATCCGTGATTTAGAGACTGCTGAGATCGCTGTTCAAGCTTCTTTGAC
GGGTCACTTAGTCATGTCGACTCTTCACACCAATACGGCAATCGGTGCGATCACGCGTCTGCGTGATATGGGCATTGAAC
CTTTCTTGATCTCTTCTTCTCTGCTGGGTGTTTTGGCTCAGCGCTTGGTTCGTACTCTGTGTAACGACTGTAAAGAACCT
TACGAAGCAGATAAAGAACAGAAAAAGCTGTTTGGTATGAAGAAGAAAGAGAGCCTCACACTTTACCATGCAAAAGGTTG
TGAAGAGTGTGGCCATAAAGGTTATCGAGGTCGTACCGGTATTCATGAGCTACTGATGATTGACGATTCGGTACAAGAGC
TGATTCACAGTGAAGCTGGAGAACAGGCAATTGAGAAAGCGATTCGTGGCACAACCCCAAGTATTCGAGATGATGGCTTG
AGCAAAGTTCTGAAAGGGGTAACGTCCTTAGAAGAAGTGATGCGAGTGACTAAGGAAGTCTAG

Upstream 100 bases:

>100_bases
TGATGGCTGACGATAATATCCCTGTGTTACCTAAATTTGGTGAAAGCATGAATCATCCTGCTGAAATCCAAGCCTTCATC
GATCAAATGGAAGCAGAATA

Downstream 100 bases:

>100_bases
TATGGCGGCATTTGAATACAAAGCGCTGGATGCCAAAGGTAAGAGCAAAAAGGGTTCGATTGAAGCGGATAATGCTCGTC
AGGCTCGCCAACGTTTAAAA

Product: type II secretory pathway ATPase EpsE

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MAELVGAARTYQRLPFSFANRYKMVLEYQHPERAPVLYYVEPLKSAAIIEVSRVVKNGFTPQAISADEFDKKLTDAYQRD
SSEARQLMEDIGADNDDFFSLAEELPQDEDLLESEDDAPIIKLINAMLGEAIKEGASDIHIETFEKSLSIRFRIDGVLRD
VLAPSRKLAPLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTA
ENHENFRKLIQRPHGIILVTGPTGSGKSTTLYAGLQELNSNERNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAIL
RQDPDVVMIGEIRDLETAEIAVQASLTGHLVMSTLHTNTAIGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCNDCKEP
YEADKEQKKLFGMKKKESLTLYHAKGCEECGHKGYRGRTGIHELLMIDDSVQELIHSEAGEQAIEKAIRGTTPSIRDDGL
SKVLKGVTSLEEVMRVTKEV

Sequences:

>Translated_500_residues
MAELVGAARTYQRLPFSFANRYKMVLEYQHPERAPVLYYVEPLKSAAIIEVSRVVKNGFTPQAISADEFDKKLTDAYQRD
SSEARQLMEDIGADNDDFFSLAEELPQDEDLLESEDDAPIIKLINAMLGEAIKEGASDIHIETFEKSLSIRFRIDGVLRD
VLAPSRKLAPLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTA
ENHENFRKLIQRPHGIILVTGPTGSGKSTTLYAGLQELNSNERNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAIL
RQDPDVVMIGEIRDLETAEIAVQASLTGHLVMSTLHTNTAIGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCNDCKEP
YEADKEQKKLFGMKKKESLTLYHAKGCEECGHKGYRGRTGIHELLMIDDSVQELIHSEAGEQAIEKAIRGTTPSIRDDGL
SKVLKGVTSLEEVMRVTKEV
>Mature_499_residues
AELVGAARTYQRLPFSFANRYKMVLEYQHPERAPVLYYVEPLKSAAIIEVSRVVKNGFTPQAISADEFDKKLTDAYQRDS
SEARQLMEDIGADNDDFFSLAEELPQDEDLLESEDDAPIIKLINAMLGEAIKEGASDIHIETFEKSLSIRFRIDGVLRDV
LAPSRKLAPLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTAE
NHENFRKLIQRPHGIILVTGPTGSGKSTTLYAGLQELNSNERNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAILR
QDPDVVMIGEIRDLETAEIAVQASLTGHLVMSTLHTNTAIGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCNDCKEPY
EADKEQKKLFGMKKKESLTLYHAKGCEECGHKGYRGRTGIHELLMIDDSVQELIHSEAGEQAIEKAIRGTTPSIRDDGLS
KVLKGVTSLEEVMRVTKEV

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=488, Percent_Identity=54.9180327868852, Blast_Score=543, Evalue=1e-155,
Organism=Escherichia coli, GI1786296, Length=383, Percent_Identity=43.6031331592689, Blast_Score=318, Evalue=4e-88,
Organism=Escherichia coli, GI87082188, Length=169, Percent_Identity=43.7869822485207, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 55614; Mature: 55482

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELVGAARTYQRLPFSFANRYKMVLEYQHPERAPVLYYVEPLKSAAIIEVSRVVKNGFT
CCCHHHHHHHHHHCCHHHHHHEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHCCCC
PQAISADEFDKKLTDAYQRDSSEARQLMEDIGADNDDFFSLAEELPQDEDLLESEDDAPI
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHCCCCCCHH
IKLINAMLGEAIKEGASDIHIETFEKSLSIRFRIDGVLRDVLAPSRKLAPLLVSRVKVMA
HHHHHHHHHHHHHCCCCCCCCEEHHCCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHH
KLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTA
HHHHHHHCCCCCCEEEEEECCEEEEEEEEECCCCCHHHHHHHHHHCCCCEEEHHHHCCCC
ENHENFRKLIQRPHGIILVTGPTGSGKSTTLYAGLQELNSNERNILTVEDPIEFDIDGIG
CCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCEECCCCCC
QTQVNPKVDMTFARGLRAILRQDPDVVMIGEIRDLETAEIAVQASLTGHLVMSTLHTNTA
CCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEHHHHHHHHHHHHHHHH
IGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCNDCKEPYEADKEQKKLFGMKKKESLT
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHCCCCCCCEE
LYHAKGCEECGHKGYRGRTGIHELLMIDDSVQELIHSEAGEQAIEKAIRGTTPSIRDDGL
EEECCCHHHHCCCCCCCCCCHHHHHEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
SKVLKGVTSLEEVMRVTKEV
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AELVGAARTYQRLPFSFANRYKMVLEYQHPERAPVLYYVEPLKSAAIIEVSRVVKNGFT
CCHHHHHHHHHHCCHHHHHHEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHCCCC
PQAISADEFDKKLTDAYQRDSSEARQLMEDIGADNDDFFSLAEELPQDEDLLESEDDAPI
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHCCCCCCHH
IKLINAMLGEAIKEGASDIHIETFEKSLSIRFRIDGVLRDVLAPSRKLAPLLVSRVKVMA
HHHHHHHHHHHHHCCCCCCCCEEHHCCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHH
KLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTA
HHHHHHHCCCCCCEEEEEECCEEEEEEEEECCCCCHHHHHHHHHHCCCCEEEHHHHCCCC
ENHENFRKLIQRPHGIILVTGPTGSGKSTTLYAGLQELNSNERNILTVEDPIEFDIDGIG
CCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCEECCCCCC
QTQVNPKVDMTFARGLRAILRQDPDVVMIGEIRDLETAEIAVQASLTGHLVMSTLHTNTA
CCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEHHHHHHHHHHHHHHHH
IGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCNDCKEPYEADKEQKKLFGMKKKESLT
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHCCCCCCCEE
LYHAKGCEECGHKGYRGRTGIHELLMIDDSVQELIHSEAGEQAIEKAIRGTTPSIRDDGL
EEECCCHHHHCCCCCCCCCCHHHHHEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
SKVLKGVTSLEEVMRVTKEV
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]