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The map label for this gene is glnA [H]

Identifier: 218708185

GI number: 218708185

Start: 114730

End: 116139

Strand: Reverse

Name: glnA [H]

Synonym: VS_0121

Alternate gene names: 218708185

Gene position: 116139-114730 (Counterclockwise)

Preceding gene: 218708186

Following gene: 218708184

Centisome position: 3.52

GC content: 46.17

Gene sequence:

>1410_bases
ATGTCAGTAGAAAATGTACTATCCCTGATCCAAGAGAACGAAGTTAAATTTATCGACTTACGTTTTACTGATACAAAAGG
TAAAGAGCAGCACATCTCTATTCCTTCTCACCAAGTTGATGCAGACTTCTTCGAAGAAGGCAAAATGTTTGACGGCTCTT
CAGTAGCTGGTTGGAAAGGCATTAATGAATCTGACATGGTAATGATGCCAGACGCAGCTTCTGCTGTACTGGACCCATTC
ACAGAAGATGCAACGCTAAACATCCGTTGTGACATCCTTGAGCCTGCAACAATGCAAGGCTACGACCGTGACCCACGCTC
TATCGCTAAACGTTCTGAAGAATACCTACGTTCTACGGGTATCGCAGACACAGTTCTAGTGGGTCCAGAGCCAGAATTCT
TCCTATTCGATGACGTTAAGTTCTCAAACGACATGTCTGGTTCTTTCTTCAAAATTGATGATGTAGAAGCAGCTTGGAAC
ACAGGCTCTGACATCGAAGGCGGTAACAAAGGTCACCGTCCTGGCGTTAAAGGCGGTTACTTCCCAGTAGCTCCTGTCGA
TTCATCTCAAGATATCCGTTCAGCAATGTGTCTAGTAATGGAAGAGATGGGCCTAGTTGTTGAAGCTCACCACCACGAAG
TTGCAACTGCGGGTCAAAACGAAATCGCAACTCGCTTCAACACGCTAACAACAAAAGCGGATGAGACTCAAATCTACAAG
TACGTTGTACACAACGTTGCACACGCATTTGGCAAAACAGCGACATTCATGCCTAAGCCACTAGTTGGTGACAACGGTTC
TGGCATGCACGTTCACATGTCATTGAACAAAGATGGACAAAACTTGTTTGCTGGTGATAAGTACGGCGGCCTATCTGAAA
TGGCTCTTTACTACATCGGTGGTGTAATCAAGCACGCTCGCGCAATCAACGCATTTGCTAACCCATCAACGAACTCATAC
AAGCGTCTTGTACCTGGTTTCGAAGCACCTGTAATGCTTGCTTACTCAGCACGTAACCGTTCTGCTTCTATCCGCATCCC
AGTAGTACCAAGCCCGAAAGCACGTCGTATCGAGCTACGTTTTGGTGACCCAGCAGCTAACCCATACCTATGCTACTCAG
CAATGCTTATGGCTGGCCTTGACGGTATTAAGAACAAGATCCACCCTGGCGAAGCTATGGATAAAGATCTATACGACCTT
CCTGCAGAAGAAGCAGCTGAAATCCCAACAGTTGCAGAATCTCTACAAGGTGCTCTAGCAGCACTTAACGAAGACCGTGA
GTTCCTAACTGCTGGTGGCGTATTCTCTGACGACTTCATCGATTCTTACATCACACTGAAATCTGACGACGTACAGCGCG
TGAACATGGCTACACACCCACTTGAGTTTGAACTGTACTACTCAGTTTAA

Upstream 100 bases:

>100_bases
GATAAATCAGCATCGATTAATGCACGTATAGACATTAATCAATGCTGGTTTCAACCGAATCGAGCTAATACCGAATTAAT
AACACTGGAGGTTATCCAAG

Downstream 100 bases:

>100_bases
TCCTTAGTCGGTTAGCATGTAATTCTTACTGCAGTAAAAGCTATTGCAACATGTAAGAAGCACTAAAAAATATTAGGCTC
GCCTCGCAGGCGGGCCTTTT

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSVENVLSLIQENEVKFIDLRFTDTKGKEQHISIPSHQVDADFFEEGKMFDGSSVAGWKGINESDMVMMPDAASAVLDPF
TEDATLNIRCDILEPATMQGYDRDPRSIAKRSEEYLRSTGIADTVLVGPEPEFFLFDDVKFSNDMSGSFFKIDDVEAAWN
TGSDIEGGNKGHRPGVKGGYFPVAPVDSSQDIRSAMCLVMEEMGLVVEAHHHEVATAGQNEIATRFNTLTTKADETQIYK
YVVHNVAHAFGKTATFMPKPLVGDNGSGMHVHMSLNKDGQNLFAGDKYGGLSEMALYYIGGVIKHARAINAFANPSTNSY
KRLVPGFEAPVMLAYSARNRSASIRIPVVPSPKARRIELRFGDPAANPYLCYSAMLMAGLDGIKNKIHPGEAMDKDLYDL
PAEEAAEIPTVAESLQGALAALNEDREFLTAGGVFSDDFIDSYITLKSDDVQRVNMATHPLEFELYYSV

Sequences:

>Translated_469_residues
MSVENVLSLIQENEVKFIDLRFTDTKGKEQHISIPSHQVDADFFEEGKMFDGSSVAGWKGINESDMVMMPDAASAVLDPF
TEDATLNIRCDILEPATMQGYDRDPRSIAKRSEEYLRSTGIADTVLVGPEPEFFLFDDVKFSNDMSGSFFKIDDVEAAWN
TGSDIEGGNKGHRPGVKGGYFPVAPVDSSQDIRSAMCLVMEEMGLVVEAHHHEVATAGQNEIATRFNTLTTKADETQIYK
YVVHNVAHAFGKTATFMPKPLVGDNGSGMHVHMSLNKDGQNLFAGDKYGGLSEMALYYIGGVIKHARAINAFANPSTNSY
KRLVPGFEAPVMLAYSARNRSASIRIPVVPSPKARRIELRFGDPAANPYLCYSAMLMAGLDGIKNKIHPGEAMDKDLYDL
PAEEAAEIPTVAESLQGALAALNEDREFLTAGGVFSDDFIDSYITLKSDDVQRVNMATHPLEFELYYSV
>Mature_468_residues
SVENVLSLIQENEVKFIDLRFTDTKGKEQHISIPSHQVDADFFEEGKMFDGSSVAGWKGINESDMVMMPDAASAVLDPFT
EDATLNIRCDILEPATMQGYDRDPRSIAKRSEEYLRSTGIADTVLVGPEPEFFLFDDVKFSNDMSGSFFKIDDVEAAWNT
GSDIEGGNKGHRPGVKGGYFPVAPVDSSQDIRSAMCLVMEEMGLVVEAHHHEVATAGQNEIATRFNTLTTKADETQIYKY
VVHNVAHAFGKTATFMPKPLVGDNGSGMHVHMSLNKDGQNLFAGDKYGGLSEMALYYIGGVIKHARAINAFANPSTNSYK
RLVPGFEAPVMLAYSARNRSASIRIPVVPSPKARRIELRFGDPAANPYLCYSAMLMAGLDGIKNKIHPGEAMDKDLYDLP
AEEAAEIPTVAESLQGALAALNEDREFLTAGGVFSDDFIDSYITLKSDDVQRVNMATHPLEFELYYSV

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=503, Percent_Identity=23.0616302186879, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1790301, Length=469, Percent_Identity=78.8912579957356, Blast_Score=796, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=289, Percent_Identity=28.3737024221453, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51488; Mature: 51357

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVENVLSLIQENEVKFIDLRFTDTKGKEQHISIPSHQVDADFFEEGKMFDGSSVAGWKG
CCHHHHHHHHHCCCEEEEEEEEECCCCCCCEECCCCCCCCCHHHHCCCCCCCCCCCCCCC
INESDMVMMPDAASAVLDPFTEDATLNIRCDILEPATMQGYDRDPRSIAKRSEEYLRSTG
CCCCCEEECCCHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
IADTVLVGPEPEFFLFDDVKFSNDMSGSFFKIDDVEAAWNTGSDIEGGNKGHRPGVKGGY
CCCEEEECCCCCEEEEECCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCC
FPVAPVDSSQDIRSAMCLVMEEMGLVVEAHHHEVATAGQNEIATRFNTLTTKADETQIYK
EECCCCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHCCCHHHHHHHHHHCCCCCHHHHHH
YVVHNVAHAFGKTATFMPKPLVGDNGSGMHVHMSLNKDGQNLFAGDKYGGLSEMALYYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHHH
GVIKHARAINAFANPSTNSYKRLVPGFEAPVMLAYSARNRSASIRIPVVPSPKARRIELR
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEECCCCCCCEEEEEE
FGDPAANPYLCYSAMLMAGLDGIKNKIHPGEAMDKDLYDLPAEEAAEIPTVAESLQGALA
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHCCHHHHHCCHHHHHHHHHHHH
ALNEDREFLTAGGVFSDDFIDSYITLKSDDVQRVNMATHPLEFELYYSV
HHCCCCCEEEECCCCCCCHHCCEEEECCCCCEEEECCCCCEEEEEEEEC
>Mature Secondary Structure 
SVENVLSLIQENEVKFIDLRFTDTKGKEQHISIPSHQVDADFFEEGKMFDGSSVAGWKG
CHHHHHHHHHCCCEEEEEEEEECCCCCCCEECCCCCCCCCHHHHCCCCCCCCCCCCCCC
INESDMVMMPDAASAVLDPFTEDATLNIRCDILEPATMQGYDRDPRSIAKRSEEYLRSTG
CCCCCEEECCCHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
IADTVLVGPEPEFFLFDDVKFSNDMSGSFFKIDDVEAAWNTGSDIEGGNKGHRPGVKGGY
CCCEEEECCCCCEEEEECCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCC
FPVAPVDSSQDIRSAMCLVMEEMGLVVEAHHHEVATAGQNEIATRFNTLTTKADETQIYK
EECCCCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHCCCHHHHHHHHHHCCCCCHHHHHH
YVVHNVAHAFGKTATFMPKPLVGDNGSGMHVHMSLNKDGQNLFAGDKYGGLSEMALYYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHHH
GVIKHARAINAFANPSTNSYKRLVPGFEAPVMLAYSARNRSASIRIPVVPSPKARRIELR
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEECCCCCCCEEEEEE
FGDPAANPYLCYSAMLMAGLDGIKNKIHPGEAMDKDLYDLPAEEAAEIPTVAESLQGALA
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHCCHHHHHCCHHHHHHHHHHHH
ALNEDREFLTAGGVFSDDFIDSYITLKSDDVQRVNMATHPLEFELYYSV
HHCCCCCEEEECCCCCCCHHCCEEEECCCCCEEEECCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]