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The map label for this gene is glnG [H]

Identifier: 218708182

GI number: 218708182

Start: 111162

End: 112565

Strand: Reverse

Name: glnG [H]

Synonym: VS_0118

Alternate gene names: 218708182

Gene position: 112565-111162 (Counterclockwise)

Preceding gene: 218708183

Following gene: 218708181

Centisome position: 3.41

GC content: 48.93

Gene sequence:

>1404_bases
ATGAGTAAGGGATACGTTTGGGTCGTCGATGACGACAGCTCTATCCGCTGGGTAGTAGAGAAAACACTTTCATCTGCGGA
TATAAAGTGTGAAACATTTGCTGATGCAGAGAGCGTATTACTTGCTCTTGAGCGCGAGACGCCAGATGTATTGGTATCTG
ATATTCGCATGCCTGGCATTGACGGTATTGAGCTTTTGCATCAGGTTCATCAACGCTCTCCCGACCTGCCAGTGATCATT
ATGACCGCGCACTCAGACCTTGATGCGGCGGTTAATGCTTATCAAAAAGGCGCTTTTGAGTATCTGCCTAAGCCGTTCGA
TATCGATGAGACACTGACTTTAGTAGAGCGAGCAATCGCCCACAGCCAAGAGCAAAAGCGAGAACAAGCCAGCGAAGCAG
TAGAAGACACCAACGCGCCAGAAATCATTGGCGAAGCGCCTGCGATGCAGGAAGTCTTCCGCGCCATTGGTCGTTTGTCT
CGGTCATCCATTTCCGTGCTAATCAATGGTGAATCAGGTACAGGTAAAGAACTAGTCGCACACGCCTTACATCGTCACAG
CCCAAGAGCGAAAAAGCCCTTTATCGCCCTGAACATGGCAGCAATTCCCAAAGATCTCATTGAATCAGAGCTGTTTGGCC
ATGAGAAAGGCGCGTTTACTGGAGCCAACAGCGTTCGCCAAGGACGCTTCGAGCAAGCCAACGGGGGTACTCTGTTTCTT
GATGAGATTGGCGACATGCCACTCGATATTCAGACTCGACTACTGCGCGTGCTGTCTGATGGCCAGTTTTATCGCGTAGG
TGGACATTCTGCGGTCAAGGTCGATGTTCGTATCGTTGCCGCAACCCACCAAGATCTTGAGCGCTTAGTACATGACGGTG
GTTTCCGTGAAGACCTTTTCCATCGACTCAACGTTATCCGAATCCATATTCCGGCACTGCGCGAACGTAAACAGGACATT
GAAAAGCTGACTCACCACTTCCTAGCATCAGCCGCTGAAGAGCTTGGTGTAGAAGTGAAGACACTACACCCAGAGACCAT
TCTGAAGCTCAATCAGCTCAATTGGCCGGGAAACGTGCGTCAGCTTGAGAACATTTGTCGTTGGTTAACAGTAATGGCAA
GTGGTAGTGAAATACTTCCTTCGGATCTGCCTCCTGAGTTGTTGGAAGAGAAGGTTGTGATCAGCGAAGGTACTGATGGT
AATTGGCAACAACTGCTGGCTAATTGGGCCAAGAGTGCGCTTGATTCCGGCGAAAAAGAGCTGCTGACTTATGCTCTACC
CGAGTTTGAACGTATATTATTAGAGGCCGCACTCAACCATACTAATGGCCATAAACAAGATGCAGCGAAAGTTTTAGGAT
GGGGAAGAAATACCCTGACTCGTAAACTCAAAGAGTTGTACTGA

Upstream 100 bases:

>100_bases
GTCTCCCACTGGAAACAACGTTTCACTGGCAACACTGAATACCGAACAATAAAAACAACAATGATTATCACAATAGATTT
GGCTGCAAAGGATTTGAATT

Downstream 100 bases:

>100_bases
TCTTTATATGCCTCTATATACATATTTTTTAAAGCTAAAAAAGTGTCGCTTGCTTTGAATAGTATCGGTACAATTACAGT
ACACTATCTATCCAAACTGT

Product: nitrogen regulation protein NR(I)

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSKGYVWVVDDDSSIRWVVEKTLSSADIKCETFADAESVLLALERETPDVLVSDIRMPGIDGIELLHQVHQRSPDLPVII
MTAHSDLDAAVNAYQKGAFEYLPKPFDIDETLTLVERAIAHSQEQKREQASEAVEDTNAPEIIGEAPAMQEVFRAIGRLS
RSSISVLINGESGTGKELVAHALHRHSPRAKKPFIALNMAAIPKDLIESELFGHEKGAFTGANSVRQGRFEQANGGTLFL
DEIGDMPLDIQTRLLRVLSDGQFYRVGGHSAVKVDVRIVAATHQDLERLVHDGGFREDLFHRLNVIRIHIPALRERKQDI
EKLTHHFLASAAEELGVEVKTLHPETILKLNQLNWPGNVRQLENICRWLTVMASGSEILPSDLPPELLEEKVVISEGTDG
NWQQLLANWAKSALDSGEKELLTYALPEFERILLEAALNHTNGHKQDAAKVLGWGRNTLTRKLKELY

Sequences:

>Translated_467_residues
MSKGYVWVVDDDSSIRWVVEKTLSSADIKCETFADAESVLLALERETPDVLVSDIRMPGIDGIELLHQVHQRSPDLPVII
MTAHSDLDAAVNAYQKGAFEYLPKPFDIDETLTLVERAIAHSQEQKREQASEAVEDTNAPEIIGEAPAMQEVFRAIGRLS
RSSISVLINGESGTGKELVAHALHRHSPRAKKPFIALNMAAIPKDLIESELFGHEKGAFTGANSVRQGRFEQANGGTLFL
DEIGDMPLDIQTRLLRVLSDGQFYRVGGHSAVKVDVRIVAATHQDLERLVHDGGFREDLFHRLNVIRIHIPALRERKQDI
EKLTHHFLASAAEELGVEVKTLHPETILKLNQLNWPGNVRQLENICRWLTVMASGSEILPSDLPPELLEEKVVISEGTDG
NWQQLLANWAKSALDSGEKELLTYALPEFERILLEAALNHTNGHKQDAAKVLGWGRNTLTRKLKELY
>Mature_466_residues
SKGYVWVVDDDSSIRWVVEKTLSSADIKCETFADAESVLLALERETPDVLVSDIRMPGIDGIELLHQVHQRSPDLPVIIM
TAHSDLDAAVNAYQKGAFEYLPKPFDIDETLTLVERAIAHSQEQKREQASEAVEDTNAPEIIGEAPAMQEVFRAIGRLSR
SSISVLINGESGTGKELVAHALHRHSPRAKKPFIALNMAAIPKDLIESELFGHEKGAFTGANSVRQGRFEQANGGTLFLD
EIGDMPLDIQTRLLRVLSDGQFYRVGGHSAVKVDVRIVAATHQDLERLVHDGGFREDLFHRLNVIRIHIPALRERKQDIE
KLTHHFLASAAEELGVEVKTLHPETILKLNQLNWPGNVRQLENICRWLTVMASGSEILPSDLPPELLEEKVVISEGTDGN
WQQLLANWAKSALDSGEKELLTYALPEFERILLEAALNHTNGHKQDAAKVLGWGRNTLTRKLKELY

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=470, Percent_Identity=71.7021276595745, Blast_Score=687, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=462, Percent_Identity=40.9090909090909, Blast_Score=347, Evalue=9e-97,
Organism=Escherichia coli, GI1790437, Length=458, Percent_Identity=39.0829694323144, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1788905, Length=462, Percent_Identity=37.8787878787879, Blast_Score=298, Evalue=6e-82,
Organism=Escherichia coli, GI1789233, Length=349, Percent_Identity=42.6934097421203, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI87082117, Length=350, Percent_Identity=38.8571428571429, Blast_Score=244, Evalue=9e-66,
Organism=Escherichia coli, GI1789087, Length=341, Percent_Identity=40.7624633431085, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1786524, Length=343, Percent_Identity=42.5655976676385, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1787583, Length=292, Percent_Identity=43.4931506849315, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI87082152, Length=252, Percent_Identity=46.8253968253968, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI87081872, Length=340, Percent_Identity=37.3529411764706, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI87081858, Length=306, Percent_Identity=31.6993464052288, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1789828, Length=224, Percent_Identity=37.0535714285714, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1789809, Length=192, Percent_Identity=28.125, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52077; Mature: 51946

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKGYVWVVDDDSSIRWVVEKTLSSADIKCETFADAESVLLALERETPDVLVSDIRMPGI
CCCCEEEEEECCCCCHHHHHHHHCCCCCEEHHHCCHHHHHHHHCCCCCHHHHHHCCCCCC
DGIELLHQVHQRSPDLPVIIMTAHSDLDAAVNAYQKGAFEYLPKPFDIDETLTLVERAIA
HHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHH
HSQEQKREQASEAVEDTNAPEIIGEAPAMQEVFRAIGRLSRSSISVLINGESGTGKELVA
HHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
HALHRHSPRAKKPFIALNMAAIPKDLIESELFGHEKGAFTGANSVRQGRFEQANGGTLFL
HHHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCEEEE
DEIGDMPLDIQTRLLRVLSDGQFYRVGGHSAVKVDVRIVAATHQDLERLVHDGGFREDLF
HHHCCCCCHHHHHHHHHHCCCCEEEECCCCEEEEEEEEEEHHHHHHHHHHHCCCCHHHHH
HRLNVIRIHIPALRERKQDIEKLTHHFLASAAEELGVEVKTLHPETILKLNQLNWPGNVR
HHHCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHCCCCCCCHH
QLENICRWLTVMASGSEILPSDLPPELLEEKVVISEGTDGNWQQLLANWAKSALDSGEKE
HHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCHHH
LLTYALPEFERILLEAALNHTNGHKQDAAKVLGWGRNTLTRKLKELY
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHC
>Mature Secondary Structure 
SKGYVWVVDDDSSIRWVVEKTLSSADIKCETFADAESVLLALERETPDVLVSDIRMPGI
CCCEEEEEECCCCCHHHHHHHHCCCCCEEHHHCCHHHHHHHHCCCCCHHHHHHCCCCCC
DGIELLHQVHQRSPDLPVIIMTAHSDLDAAVNAYQKGAFEYLPKPFDIDETLTLVERAIA
HHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHH
HSQEQKREQASEAVEDTNAPEIIGEAPAMQEVFRAIGRLSRSSISVLINGESGTGKELVA
HHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
HALHRHSPRAKKPFIALNMAAIPKDLIESELFGHEKGAFTGANSVRQGRFEQANGGTLFL
HHHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCEEEE
DEIGDMPLDIQTRLLRVLSDGQFYRVGGHSAVKVDVRIVAATHQDLERLVHDGGFREDLF
HHHCCCCCHHHHHHHHHHCCCCEEEECCCCEEEEEEEEEEHHHHHHHHHHHCCCCHHHHH
HRLNVIRIHIPALRERKQDIEKLTHHFLASAAEELGVEVKTLHPETILKLNQLNWPGNVR
HHHCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHCCCCCCCHH
QLENICRWLTVMASGSEILPSDLPPELLEEKVVISEGTDGNWQQLLANWAKSALDSGEKE
HHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCHHH
LLTYALPEFERILLEAALNHTNGHKQDAAKVLGWGRNTLTRKLKELY
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]