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The map label for this gene is rep [H]

Identifier: 218708138

GI number: 218708138

Start: 58199

End: 60217

Strand: Direct

Name: rep [H]

Synonym: VS_0066

Alternate gene names: 218708138

Gene position: 58199-60217 (Clockwise)

Preceding gene: 218708133

Following gene: 218708140

Centisome position: 1.76

GC content: 46.11

Gene sequence:

>2019_bases
ATGAAACTGAACCCAAGACAAGATGAAGCCGTGAAATATGTTTCGGGCCCCTGTTTAGTATTAGCAGGCGCTGGATCGGG
TAAAACACGTGTTATCACGAATAAGATTGCTTACTTGGTTCAAGAGTGTGGTTACAAGGCACGCAATATTGCAGCGGTAA
CTTTTACCAATAAAGCGGCACGCGAGATGAAAGAACGTGTGGGGCAAACTCTGGGTAAAGGTGAATCGAAAGGCCTGATC
GTTTCGACTTTTCATACCATGGGCTTAACTATCATTCGTCGTGAATACAAAGCGCTGGGTCTGAAAGCGGGCTTCTCTCT
ATTTGATGACCAAGACCAGTTGGCGCTATTAAAAGAGCTAACAGAGAGACAGATCGATGGTGATAAGGATCTGCTGCGTG
CTTTAATGAGCACCATTTCGAATTGGAAGAATGACATGCTGACGCCAGATCAGGCGAAAGCTCGAGCTCAAGGCGAACAA
GAGCAGTTGTTTGCATTCTGTTTTGAGATGTACCAGAAACAGATGAAGGCATACAACGCGCTCGATTTTGATGATCTGAT
TGCGATGCCAGTATTACTTCTGAAAACCAATCAAGAAGTGCGTGAGCGCTGGCAGTCTCGTATTCGCTATTTACTTGTCG
ATGAGTATCAAGATACCAACACCAGCCAATATGAATTGGTGCGTCTGCTTGTGGGAGAGCGTGGTCGTTTGACGGTCGTA
GGTGACGATGACCAATCTATTTACTCATGGCGTGGTGCAAAACCGCAAAACTTGGTGCTGTTAGGTGAGGATTACCCGAA
CTTACGCTTGATCAAACTAGAGCAGAACTACCGCTCAACGAGTCGAATTTTGCGTGCAGCGAATATCTTGATCGCTAATA
ACCCACACGTTTATGAAAAATCACTGTTCTCTGAGATCCCGGACGGTGAAAAGCTCAAGGTACTGAATGCTAAGAATGAA
GAGCATGAAGCAGAGCGTATTACAGGTGAGATCATCGCTCACAAGTTTTTAAACCGCACTGAATATAAAGATTACGCAGT
GCTGTACCGTGGTAACCACCAATCTCGCTTGATTGAAAAAGCGTTGATGCAGAACCGTGTGCCTTACAAAATTTCTGGCG
GTACGTCTTTCTTCGCTAGAGCTGAGATTAAAGACATCATGGCTTACCTCCGCGTGTTGGTGAACCCTGATGATGACAAC
GCGTTTCTACGTATTGTAAACACACCACGTCGTGAGATTGGCCCGGTGACGCTTGAGAAGTTAGGCAGCTACGCCAACAT
GCGTGGCAAGAGCTTGTTTGAAGCGAGCTTTGAGATGGGTTTAGAGCAAACTTTGACTGGTCGTGGTTTAGAAAACCTGC
GCCGTTTCAGCGATTGGATTGTTCGTATTTCTGATAATGCTGAACGTGGCAATACCGTTGAGGCTGTTCGCTCTTTGGTT
CGTGATATTAACTATGAAGATTGGTTGTACGAAACCTCGGCAAGCCCGAAGGCTGCAGAGATGAGAATGAAGAACGTTTC
TGATCTTTATAGTTGGATCGTTGCTGACCTTGAGGGTGACAACTACGACAAAGAAGAGAAGACCCTACGAGAAGTGGTTC
AACGTTTAACGCTACGTGACATGATGGAGCGTGGTGAAGATGATGACGATGCAGACCAAGTTCAATTAATGACATTGCAC
GCATCGAAAGGCCTAGAGTTTCCGTATGTGTTCCTGATGGGAGCCGAAGAGGGTATCTTGCCACACCAAACCAGTGTCGA
TGAAGGTAACGTAGAAGAAGAACGTCGTCTTATGTATGTAGGGATTACTCGCGCGCAGAAAGAGCTGACCTTCACTAAAT
GTCGTGAACGTCGTCAGTATGGCGAATTGATAAAGCCAACACAAAGCCGATTCCTTGATGAACTGCCGCATGAAGATGTG
GAGTGGGAAAGCGTGAAGAAGCCTCAATCCGCAGAAGAACGAATGGAGAAAGGACAGGCACACATTGCCAACATTCGTGC
GATGTTCAATAAGAAGTGA

Upstream 100 bases:

>100_bases
ATATAAAATTCTTGCATAAGAGAGCGTAGCATTCGAGGTGTTGCAGCATTATTATAGGCGCGCAATTGAATTCGCCCCGA
TAGACCAAATTGAGAACCAA

Downstream 100 bases:

>100_bases
TGTTTGGCTCTAAATCGAGACCAATAAAAAGGTGACCATGAGGTCACCTTTTTTGAATTTGGTATTTTTAATTACAGATC
GGTAAATTACAGACCGGCAA

Product: ATP-dependent DNA helicase rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 672; Mature: 672

Protein sequence:

>672_residues
MKLNPRQDEAVKYVSGPCLVLAGAGSGKTRVITNKIAYLVQECGYKARNIAAVTFTNKAAREMKERVGQTLGKGESKGLI
VSTFHTMGLTIIRREYKALGLKAGFSLFDDQDQLALLKELTERQIDGDKDLLRALMSTISNWKNDMLTPDQAKARAQGEQ
EQLFAFCFEMYQKQMKAYNALDFDDLIAMPVLLLKTNQEVRERWQSRIRYLLVDEYQDTNTSQYELVRLLVGERGRLTVV
GDDDQSIYSWRGAKPQNLVLLGEDYPNLRLIKLEQNYRSTSRILRAANILIANNPHVYEKSLFSEIPDGEKLKVLNAKNE
EHEAERITGEIIAHKFLNRTEYKDYAVLYRGNHQSRLIEKALMQNRVPYKISGGTSFFARAEIKDIMAYLRVLVNPDDDN
AFLRIVNTPRREIGPVTLEKLGSYANMRGKSLFEASFEMGLEQTLTGRGLENLRRFSDWIVRISDNAERGNTVEAVRSLV
RDINYEDWLYETSASPKAAEMRMKNVSDLYSWIVADLEGDNYDKEEKTLREVVQRLTLRDMMERGEDDDDADQVQLMTLH
ASKGLEFPYVFLMGAEEGILPHQTSVDEGNVEEERRLMYVGITRAQKELTFTKCRERRQYGELIKPTQSRFLDELPHEDV
EWESVKKPQSAEERMEKGQAHIANIRAMFNKK

Sequences:

>Translated_672_residues
MKLNPRQDEAVKYVSGPCLVLAGAGSGKTRVITNKIAYLVQECGYKARNIAAVTFTNKAAREMKERVGQTLGKGESKGLI
VSTFHTMGLTIIRREYKALGLKAGFSLFDDQDQLALLKELTERQIDGDKDLLRALMSTISNWKNDMLTPDQAKARAQGEQ
EQLFAFCFEMYQKQMKAYNALDFDDLIAMPVLLLKTNQEVRERWQSRIRYLLVDEYQDTNTSQYELVRLLVGERGRLTVV
GDDDQSIYSWRGAKPQNLVLLGEDYPNLRLIKLEQNYRSTSRILRAANILIANNPHVYEKSLFSEIPDGEKLKVLNAKNE
EHEAERITGEIIAHKFLNRTEYKDYAVLYRGNHQSRLIEKALMQNRVPYKISGGTSFFARAEIKDIMAYLRVLVNPDDDN
AFLRIVNTPRREIGPVTLEKLGSYANMRGKSLFEASFEMGLEQTLTGRGLENLRRFSDWIVRISDNAERGNTVEAVRSLV
RDINYEDWLYETSASPKAAEMRMKNVSDLYSWIVADLEGDNYDKEEKTLREVVQRLTLRDMMERGEDDDDADQVQLMTLH
ASKGLEFPYVFLMGAEEGILPHQTSVDEGNVEEERRLMYVGITRAQKELTFTKCRERRQYGELIKPTQSRFLDELPHEDV
EWESVKKPQSAEERMEKGQAHIANIRAMFNKK
>Mature_672_residues
MKLNPRQDEAVKYVSGPCLVLAGAGSGKTRVITNKIAYLVQECGYKARNIAAVTFTNKAAREMKERVGQTLGKGESKGLI
VSTFHTMGLTIIRREYKALGLKAGFSLFDDQDQLALLKELTERQIDGDKDLLRALMSTISNWKNDMLTPDQAKARAQGEQ
EQLFAFCFEMYQKQMKAYNALDFDDLIAMPVLLLKTNQEVRERWQSRIRYLLVDEYQDTNTSQYELVRLLVGERGRLTVV
GDDDQSIYSWRGAKPQNLVLLGEDYPNLRLIKLEQNYRSTSRILRAANILIANNPHVYEKSLFSEIPDGEKLKVLNAKNE
EHEAERITGEIIAHKFLNRTEYKDYAVLYRGNHQSRLIEKALMQNRVPYKISGGTSFFARAEIKDIMAYLRVLVNPDDDN
AFLRIVNTPRREIGPVTLEKLGSYANMRGKSLFEASFEMGLEQTLTGRGLENLRRFSDWIVRISDNAERGNTVEAVRSLV
RDINYEDWLYETSASPKAAEMRMKNVSDLYSWIVADLEGDNYDKEEKTLREVVQRLTLRDMMERGEDDDDADQVQLMTLH
ASKGLEFPYVFLMGAEEGILPHQTSVDEGNVEEERRLMYVGITRAQKELTFTKCRERRQYGELIKPTQSRFLDELPHEDV
EWESVKKPQSAEERMEKGQAHIANIRAMFNKK

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=672, Percent_Identity=70.6845238095238, Blast_Score=993, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=644, Percent_Identity=40.2173913043478, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI1787196, Length=351, Percent_Identity=29.3447293447293, Blast_Score=105, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6322369, Length=708, Percent_Identity=28.2485875706215, Blast_Score=211, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77306; Mature: 77306

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNPRQDEAVKYVSGPCLVLAGAGSGKTRVITNKIAYLVQECGYKARNIAAVTFTNKAA
CCCCCCCHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHCEEEEEECCHHH
REMKERVGQTLGKGESKGLIVSTFHTMGLTIIRREYKALGLKAGFSLFDDQDQLALLKEL
HHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
TERQIDGDKDLLRALMSTISNWKNDMLTPDQAKARAQGEQEQLFAFCFEMYQKQMKAYNA
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
LDFDDLIAMPVLLLKTNQEVRERWQSRIRYLLVDEYQDTNTSQYELVRLLVGERGRLTVV
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCCCEEEE
GDDDQSIYSWRGAKPQNLVLLGEDYPNLRLIKLEQNYRSTSRILRAANILIANNPHVYEK
ECCCCHHHHCCCCCCCEEEEECCCCCCEEEEEEECCHHHHHHHHHHHHEEEECCCCHHHH
SLFSEIPDGEKLKVLNAKNEEHEAERITGEIIAHKFLNRTEYKDYAVLYRGNHQSRLIEK
HHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
ALMQNRVPYKISGGTSFFARAEIKDIMAYLRVLVNPDDDNAFLRIVNTPRREIGPVTLEK
HHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHCCCHHHHH
LGSYANMRGKSLFEASFEMGLEQTLTGRGLENLRRFSDWIVRISDNAERGNTVEAVRSLV
HCCHHHCCCHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHEEEECCCCCCCCHHHHHHHHH
RDINYEDWLYETSASPKAAEMRMKNVSDLYSWIVADLEGDNYDKEEKTLREVVQRLTLRD
HHCCCHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
MMERGEDDDDADQVQLMTLHASKGLEFPYVFLMGAEEGILPHQTSVDEGNVEEERRLMYV
HHHCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHEEEEE
GITRAQKELTFTKCRERRQYGELIKPTQSRFLDELPHEDVEWESVKKPQSAEERMEKGQA
EEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHCHH
HIANIRAMFNKK
HHHHHHHHHCCC
>Mature Secondary Structure
MKLNPRQDEAVKYVSGPCLVLAGAGSGKTRVITNKIAYLVQECGYKARNIAAVTFTNKAA
CCCCCCCHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHCEEEEEECCHHH
REMKERVGQTLGKGESKGLIVSTFHTMGLTIIRREYKALGLKAGFSLFDDQDQLALLKEL
HHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
TERQIDGDKDLLRALMSTISNWKNDMLTPDQAKARAQGEQEQLFAFCFEMYQKQMKAYNA
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
LDFDDLIAMPVLLLKTNQEVRERWQSRIRYLLVDEYQDTNTSQYELVRLLVGERGRLTVV
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCCCEEEE
GDDDQSIYSWRGAKPQNLVLLGEDYPNLRLIKLEQNYRSTSRILRAANILIANNPHVYEK
ECCCCHHHHCCCCCCCEEEEECCCCCCEEEEEEECCHHHHHHHHHHHHEEEECCCCHHHH
SLFSEIPDGEKLKVLNAKNEEHEAERITGEIIAHKFLNRTEYKDYAVLYRGNHQSRLIEK
HHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
ALMQNRVPYKISGGTSFFARAEIKDIMAYLRVLVNPDDDNAFLRIVNTPRREIGPVTLEK
HHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHCCCHHHHH
LGSYANMRGKSLFEASFEMGLEQTLTGRGLENLRRFSDWIVRISDNAERGNTVEAVRSLV
HCCHHHCCCHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHEEEECCCCCCCCHHHHHHHHH
RDINYEDWLYETSASPKAAEMRMKNVSDLYSWIVADLEGDNYDKEEKTLREVVQRLTLRD
HHCCCHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
MMERGEDDDDADQVQLMTLHASKGLEFPYVFLMGAEEGILPHQTSVDEGNVEEERRLMYV
HHHCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHEEEEE
GITRAQKELTFTKCRERRQYGELIKPTQSRFLDELPHEDVEWESVKKPQSAEERMEKGQA
EEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHCHH
HIANIRAMFNKK
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]