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The map label for this gene is dnaN [H]

Identifier: 218708099

GI number: 218708099

Start: 9001

End: 10101

Strand: Direct

Name: dnaN [H]

Synonym: VS_0011

Alternate gene names: 218708099

Gene position: 9001-10101 (Clockwise)

Preceding gene: 218708098

Following gene: 218708100

Centisome position: 0.27

GC content: 45.78

Gene sequence:

>1101_bases
ATGAAATTTACCATTGAACGTAGTCACCTGATTAAGCCATTACAACAAGTATCTGGCGCACTCGGTGGCAGGCCAACGCT
TCCAATTCTAGGAAATCTACTGATTAAAGTAGAAGATAACGTGTTGTCGATGACAGCAACGGATCTAGAAGTTGAACTGA
TCAGCCGTGTAACGCTTGAAGGTGATTTCGAAGCGGGCAGCATTACTGTACCTTCACGTAAATTCCTAGATATCTGTCGT
GGCTTGCCAGACAGCTCAGTGATCACTGTGGTATTGGATGGCGATCGTATTCAGGTTCGTTCTGGTCGTAGCCGCTTCTC
ATTAGCAACATTACCTGCGGCAGATTTTCCAAACATTGAAGACTGGAGCAGTGAAGTTGAAGTGTCAGTGACACAAGCTG
AGCTACGCGGTCTTATCGAGAAAACCCAATTCTCAATGGCGAACCAAGATGTTCGTTATTACCTTAACGGTATGTTGTTT
GAGATTGAAGGTTCAATCCTGCGAAGTGTCGCAACTGATGGTCACCGTATGGCGGTATCTCAAGCACAATTGGGTGCCGA
TTTTGCTCAGAAGCAGATCATTGTTCCTCGTAAAGGTGTGCAAGAGTTAGTGAAGCTACTGGATGCACCAGAGCAACCGG
TAACGCTGCAGATTGGTAATTCTAATGTGCGTGCTGAAGTGAACAACTATGTCTTCACTTCTAAGCTGGTTGATGGTCGT
TTCCCTGATTATCGCCGCGTAATGCCGCAAAATACTACCAAGACACTTGAAGCTGGTTGTGATGAATTACGTTCAGCATT
TTCTCGTGCTGCGATTCTGTCGAACGAAAAATTCCGTGGTGTTCGCGTTAATCTTGTCGATAGCGAGATGCGCATTACCG
CGAACAACCCAGAACAAGAAGAAGCGGAAGAAGTGTTAGATGTTAACTTCGACGGTGACGCGCTAGAGATTGGCTTCAAC
GTAAGCTACGTATTGGATGTTCTGAACACACTACGTTGTGAACAAGTTCGCATCTCAATGTCAGACGCCAATGCGAGTGC
TCTTATCGAGAACGCACAAGACGACAGCGCGATGTACGTTGTTATGCCTATTCGCTTATAA

Upstream 100 bases:

>100_bases
TCGTACCCTTTCTTCTTAATACACAGTATTCTTAACCTTAATAAGCAGAATCTTGGCCACGTACTATTACGCAGTGCCAT
TTAGACCGTAAGAGCAAGAT

Downstream 100 bases:

>100_bases
GCGTTGTTTGTGATGTTTACTGATCAACAACGTTTACCTATCGAGTCGTGATTGAAGATTAATATGAAGACGTTATGCCT
TTATCGCGCTTAATCGTTAA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKFTIERSHLIKPLQQVSGALGGRPTLPILGNLLIKVEDNVLSMTATDLEVELISRVTLEGDFEAGSITVPSRKFLDICR
GLPDSSVITVVLDGDRIQVRSGRSRFSLATLPAADFPNIEDWSSEVEVSVTQAELRGLIEKTQFSMANQDVRYYLNGMLF
EIEGSILRSVATDGHRMAVSQAQLGADFAQKQIIVPRKGVQELVKLLDAPEQPVTLQIGNSNVRAEVNNYVFTSKLVDGR
FPDYRRVMPQNTTKTLEAGCDELRSAFSRAAILSNEKFRGVRVNLVDSEMRITANNPEQEEAEEVLDVNFDGDALEIGFN
VSYVLDVLNTLRCEQVRISMSDANASALIENAQDDSAMYVVMPIRL

Sequences:

>Translated_366_residues
MKFTIERSHLIKPLQQVSGALGGRPTLPILGNLLIKVEDNVLSMTATDLEVELISRVTLEGDFEAGSITVPSRKFLDICR
GLPDSSVITVVLDGDRIQVRSGRSRFSLATLPAADFPNIEDWSSEVEVSVTQAELRGLIEKTQFSMANQDVRYYLNGMLF
EIEGSILRSVATDGHRMAVSQAQLGADFAQKQIIVPRKGVQELVKLLDAPEQPVTLQIGNSNVRAEVNNYVFTSKLVDGR
FPDYRRVMPQNTTKTLEAGCDELRSAFSRAAILSNEKFRGVRVNLVDSEMRITANNPEQEEAEEVLDVNFDGDALEIGFN
VSYVLDVLNTLRCEQVRISMSDANASALIENAQDDSAMYVVMPIRL
>Mature_366_residues
MKFTIERSHLIKPLQQVSGALGGRPTLPILGNLLIKVEDNVLSMTATDLEVELISRVTLEGDFEAGSITVPSRKFLDICR
GLPDSSVITVVLDGDRIQVRSGRSRFSLATLPAADFPNIEDWSSEVEVSVTQAELRGLIEKTQFSMANQDVRYYLNGMLF
EIEGSILRSVATDGHRMAVSQAQLGADFAQKQIIVPRKGVQELVKLLDAPEQPVTLQIGNSNVRAEVNNYVFTSKLVDGR
FPDYRRVMPQNTTKTLEAGCDELRSAFSRAAILSNEKFRGVRVNLVDSEMRITANNPEQEEAEEVLDVNFDGDALEIGFN
VSYVLDVLNTLRCEQVRISMSDANASALIENAQDDSAMYVVMPIRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=366, Percent_Identity=64.7540983606557, Blast_Score=511, Evalue=1e-146,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40515; Mature: 40515

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTIERSHLIKPLQQVSGALGGRPTLPILGNLLIKVEDNVLSMTATDLEVELISRVTLE
CEEEECHHHHHHHHHHHHHHCCCCCCHHHHCCEEEEEECCEEEEEECCHHEEEHHEEEEC
GDFEAGSITVPSRKFLDICRGLPDSSVITVVLDGDRIQVRSGRSRFSLATLPAADFPNIE
CCCCCCCEECCCHHHHHHHCCCCCCCEEEEEECCCEEEEECCCCEEEEEECCCCCCCCCC
DWSSEVEVSVTQAELRGLIEKTQFSMANQDVRYYLNGMLFEIEGSILRSVATDGHRMAVS
CCCCCEEEEEEHHHHHHHHHHHHHHHCCCHHHHHHCCEEEEECHHHHHHHHCCCCCEEHH
QAQLGADFAQKQIIVPRKGVQELVKLLDAPEQPVTLQIGNSNVRAEVNNYVFTSKLVDGR
HHHHCHHHHHCEEECCHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCEEEEEHHHCCC
FPDYRRVMPQNTTKTLEAGCDELRSAFSRAAILSNEKFRGVRVNLVDSEMRITANNPEQE
CCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEECCCCCHH
EAEEVLDVNFDGDALEIGFNVSYVLDVLNTLRCEQVRISMSDANASALIENAQDDSAMYV
HHHHHEECCCCCCEEEECCCHHHHHHHHHHHCEEEEEEEECCCCHHHHHCCCCCCCEEEE
VMPIRL
EEEECC
>Mature Secondary Structure
MKFTIERSHLIKPLQQVSGALGGRPTLPILGNLLIKVEDNVLSMTATDLEVELISRVTLE
CEEEECHHHHHHHHHHHHHHCCCCCCHHHHCCEEEEEECCEEEEEECCHHEEEHHEEEEC
GDFEAGSITVPSRKFLDICRGLPDSSVITVVLDGDRIQVRSGRSRFSLATLPAADFPNIE
CCCCCCCEECCCHHHHHHHCCCCCCCEEEEEECCCEEEEECCCCEEEEEECCCCCCCCCC
DWSSEVEVSVTQAELRGLIEKTQFSMANQDVRYYLNGMLFEIEGSILRSVATDGHRMAVS
CCCCCEEEEEEHHHHHHHHHHHHHHHCCCHHHHHHCCEEEEECHHHHHHHHCCCCCEEHH
QAQLGADFAQKQIIVPRKGVQELVKLLDAPEQPVTLQIGNSNVRAEVNNYVFTSKLVDGR
HHHHCHHHHHCEEECCHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCEEEEEHHHCCC
FPDYRRVMPQNTTKTLEAGCDELRSAFSRAAILSNEKFRGVRVNLVDSEMRITANNPEQE
CCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEECCCCCHH
EAEEVLDVNFDGDALEIGFNVSYVLDVLNTLRCEQVRISMSDANASALIENAQDDSAMYV
HHHHHEECCCCCCEEEECCCHHHHHHHHHHHCEEEEEEEECCCCHHHHHCCCCCCCEEEE
VMPIRL
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]