Click here to switch to the map view.

The map label for this gene is recF

Identifier: 218708100

GI number: 218708100

Start: 10176

End: 11255

Strand: Direct

Name: recF

Synonym: VS_0012

Alternate gene names: 218708100

Gene position: 10176-11255 (Clockwise)

Preceding gene: 218708099

Following gene: 218708101

Centisome position: 0.31

GC content: 45.37

Gene sequence:

>1080_bases
ATGCCTTTATCGCGCTTAATCGTTAAGCAGTTTAGAAATATAGAAGCCTGTGACATTCAACCGTCATCAGGCTTTAACTT
TCTTATAGGGGCTAACGGGAGCGGAAAAACCAGTGTCCTTGAAGCGGTATATCTGCTTGGACATGGTCGCTCATTTAAGA
GTTCACTCTCCGGCCGTATCATACAAAACGAGTGCAGTGAGCTGTTTGTACATGGCCGTTTTATGACCTCGGATCAATTT
GAGCTGCCAATTGGCATTAATAAGCAGCGCGATGGCACAACAGAGGTTAAAATAAGCGGTCAAACTGGGCAAAAATTGGC
TCAGTTAGCTCAGGTTTTACCTTTGCAGTTGATTCACCCTGAAGGGTTTGATTTACTAACCGATGGACCTAAGCATCGCC
GCGCATTTATTGATTGGGGAGTATTCCACAGTGAGTCCGGCTTTTATGATGCATGGGGCAGGGTAAAGCGCCTCAATAAG
CAGCGAAACGCCTTATTGAAAACGGCAACACACTATCGAGAACTGAGCTATTGGGACCAAGAATTGGCCCGTTTAGCGGA
AAGTATCAGCCAGTGGCGTGCCACTTACGTTGAACAGCTTAAAGAAGTCGCCGAAGAAATATGTGCGACCTTCCTTCCTG
AGTTTGAGATAAAGATTAACTATTATCGTGGTTGGGACAAAGACACCCCATACGCCGAGATTTTAGAAAAGAATTTTGAA
AGGGATCAGCAGCTTGGTTACACCTTTAGTGGGCCAAACAAAGCGGATCTGAAGATAAAAGTGAACGGCACTCCAGTGGA
AGATGTCTTGTCACGAGGTCAATTGAAGTTGATGGTGTGCGCGCTGCGAGTAGCACAAGGGCAACACCTCACTCAAATGA
CAGGTAAGCAGTGCATCTATTTGATAGATGACTTCGCTTCCGAATTAGATAGCCAACGCCGAGCACGTCTTGCTGAGTGT
TTAAAGGCGACCCAGGCTCAAGTTTTTGTAAGCTCTATTACCGCTGATCAGATTGCAGATATGCATGACGAAAATAGCAG
GATGTTTCATGTGGAACATGGCAAAATAGAGCAAGGATAA

Upstream 100 bases:

>100_bases
ACGTTGTTATGCCTATTCGCTTATAAGCGTTGTTTGTGATGTTTACTGATCAACAACGTTTACCTATCGAGTCGTGATTG
AAGATTAATATGAAGACGTT

Downstream 100 bases:

>100_bases
TTAGTCAGAGAGTAACTATGTCAGATAATTACGATTCATCGAGTATTAAAGTACTGAAGGGTCTGGATGCGGTACGTAAG
CGTCCTGGAATGTACATTGG

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MPLSRLIVKQFRNIEACDIQPSSGFNFLIGANGSGKTSVLEAVYLLGHGRSFKSSLSGRIIQNECSELFVHGRFMTSDQF
ELPIGINKQRDGTTEVKISGQTGQKLAQLAQVLPLQLIHPEGFDLLTDGPKHRRAFIDWGVFHSESGFYDAWGRVKRLNK
QRNALLKTATHYRELSYWDQELARLAESISQWRATYVEQLKEVAEEICATFLPEFEIKINYYRGWDKDTPYAEILEKNFE
RDQQLGYTFSGPNKADLKIKVNGTPVEDVLSRGQLKLMVCALRVAQGQHLTQMTGKQCIYLIDDFASELDSQRRARLAEC
LKATQAQVFVSSITADQIADMHDENSRMFHVEHGKIEQG

Sequences:

>Translated_359_residues
MPLSRLIVKQFRNIEACDIQPSSGFNFLIGANGSGKTSVLEAVYLLGHGRSFKSSLSGRIIQNECSELFVHGRFMTSDQF
ELPIGINKQRDGTTEVKISGQTGQKLAQLAQVLPLQLIHPEGFDLLTDGPKHRRAFIDWGVFHSESGFYDAWGRVKRLNK
QRNALLKTATHYRELSYWDQELARLAESISQWRATYVEQLKEVAEEICATFLPEFEIKINYYRGWDKDTPYAEILEKNFE
RDQQLGYTFSGPNKADLKIKVNGTPVEDVLSRGQLKLMVCALRVAQGQHLTQMTGKQCIYLIDDFASELDSQRRARLAEC
LKATQAQVFVSSITADQIADMHDENSRMFHVEHGKIEQG
>Mature_358_residues
PLSRLIVKQFRNIEACDIQPSSGFNFLIGANGSGKTSVLEAVYLLGHGRSFKSSLSGRIIQNECSELFVHGRFMTSDQFE
LPIGINKQRDGTTEVKISGQTGQKLAQLAQVLPLQLIHPEGFDLLTDGPKHRRAFIDWGVFHSESGFYDAWGRVKRLNKQ
RNALLKTATHYRELSYWDQELARLAESISQWRATYVEQLKEVAEEICATFLPEFEIKINYYRGWDKDTPYAEILEKNFER
DQQLGYTFSGPNKADLKIKVNGTPVEDVLSRGQLKLMVCALRVAQGQHLTQMTGKQCIYLIDDFASELDSQRRARLAECL
KATQAQVFVSSITADQIADMHDENSRMFHVEHGKIEQG

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=356, Percent_Identity=57.5842696629214, Blast_Score=441, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_VIBSL (B7VGI6)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002415721.1
- GeneID:   7159710
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0012
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 40768; Mature: 40637

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSRLIVKQFRNIEACDIQPSSGFNFLIGANGSGKTSVLEAVYLLGHGRSFKSSLSGRI
CCHHHHHHHHHCCCCEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHCCCH
IQNECSELFVHGRFMTSDQFELPIGINKQRDGTTEVKISGQTGQKLAQLAQVLPLQLIHP
HHHHHHHHHHCCEEECCCCEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCEEECC
EGFDLLTDGPKHRRAFIDWGVFHSESGFYDAWGRVKRLNKQRNALLKTATHYRELSYWDQ
CCCCCCCCCHHHHEEEEEECEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELARLAESISQWRATYVEQLKEVAEEICATFLPEFEIKINYYRGWDKDTPYAEILEKNFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHH
RDQQLGYTFSGPNKADLKIKVNGTPVEDVLSRGQLKLMVCALRVAQGQHLTQMTGKQCIY
HHHHCCCEECCCCCCEEEEEECCCCHHHHHCCCCHHHHHHHHHHHCCCHHHHHCCCCEEH
LIDDFASELDSQRRARLAECLKATQAQVFVSSITADQIADMHDENSRMFHVEHGKIEQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
PLSRLIVKQFRNIEACDIQPSSGFNFLIGANGSGKTSVLEAVYLLGHGRSFKSSLSGRI
CHHHHHHHHHCCCCEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHCCCH
IQNECSELFVHGRFMTSDQFELPIGINKQRDGTTEVKISGQTGQKLAQLAQVLPLQLIHP
HHHHHHHHHHCCEEECCCCEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCEEECC
EGFDLLTDGPKHRRAFIDWGVFHSESGFYDAWGRVKRLNKQRNALLKTATHYRELSYWDQ
CCCCCCCCCHHHHEEEEEECEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELARLAESISQWRATYVEQLKEVAEEICATFLPEFEIKINYYRGWDKDTPYAEILEKNFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHH
RDQQLGYTFSGPNKADLKIKVNGTPVEDVLSRGQLKLMVCALRVAQGQHLTQMTGKQCIY
HHHHCCCEECCCCCCEEEEEECCCCHHHHHCCCCHHHHHHHHHHHCCCHHHHHCCCCEEH
LIDDFASELDSQRRARLAECLKATQAQVFVSSITADQIADMHDENSRMFHVEHGKIEQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA