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The map label for this gene is trmE [H]

Identifier: 218708090

GI number: 218708090

Start: 852

End: 2255

Strand: Reverse

Name: trmE [H]

Synonym: VS_0002

Alternate gene names: 218708090

Gene position: 2255-852 (Counterclockwise)

Preceding gene: 218708091

Following gene: 218708089

Centisome position: 0.07

GC content: 48.22

Gene sequence:

>1404_bases
GTGAATGCCCTCTTTGGCTTTCACATGCTAGCAGGCACACCTATGACTACAGATACGATTGTTGCTCAAGCGACCGCGCC
AGGCCGTGGTGGCGTCGGTATTATCCGCGTTTCCGGCCCATTGGCGACTCAGGTTGCCCTAGAAGTAACAGGAAAAACAC
TTAAGCCTCGCTACGCTGAGTACCTGCCGTTTAAATCTCACGATGGCATCGAACTCGACCAAGGTATCGCGCTTTTCTTC
CCTAACCCACATTCGTTTACTGGCGAAGACGTGTTAGAGCTGCAAGGCCACGGCGGTCCGGTGGTGATGGATATGCTAAT
CAAACGAATCCTCGCGATCTCAGGCGTGCGTGCTGCGCGTCCGGGTGAGTTTTCAGAGCGTGCCTTCTTAAACGACAAGA
TGGATTTAACTCAAGCCGAAGCGATTGCTGACCTGATTGACGCAAGCTCAGAAGAAGCGGCGAAATCTGCACTACAATCC
CTGCAAGGTGAATTCTCAAAGCGTATTAATACACTGGTAGATTCCCTGATTTACCTAAGAATCTACGTTGAAGCGGCTAT
CGACTTCCCTGAAGAAGAAATTGATTTCCTTGCTGACGGTAAAGTAAGTGCTGACTTACAGGCTATCATCGACAATCTCG
AAGCTGTGCGCCAAGAAGCCAATCAAGGTGCCATCATGCGTGAAGGTATGAAAGTCGTGATTGCAGGTCGCCCAAATGCG
GGTAAATCGAGCCTATTAAATGCATTGTCAGGCAAAGAATCTGCCATTGTGACTGATATTGCCGGCACTACGCGTGATGT
ACTGCGTGAACATATCCATATTGATGGCATGCCACTGCATATTATTGATACCGCAGGCCTACGTGAGGCCTCTGATGAAG
TAGAAAAGATAGGTATTGAACGTGCTTGGGAAGAAATAGCTCAGGCAGACCGAGTTTTATTTATGGTTGATGGCACAACA
ACAGACGCAACCGACCCGAAAGATATCTGGCCAGATTTCGTTGATCGTCTACCAGACAACATCGGAATGACGGTAATTCG
TAATAAAGCGGATCAAACCAGTGAAGAGTTAGGCATTTGTCATGTTAACGATCCAACCCTGATTCGCTTATCAGCCAAAA
CAGGCCAAGGTGTCGACGCTCTGCGTAGTCATCTAAAGGATTGTATGGGCTTTGCTGGTGGTCATGAAGGTGGCTTTATG
GCACGTCGTCGTCACTTAGATGCCCTAGAACGAGCATCAGAACACCTAGATATCGGCCAACAGCAGCTTGAAGGCTACAT
GGCGGGTGAGATTTTAGCGGAAGAGCTTCGAATTACTCAGCAGCACCTCAATGAGATCACGGGTGAGTTCAGTTCAGACG
ATCTTTTAGGTCGCATCTTCTCTTCATTCTGTATTGGTAAATAA

Upstream 100 bases:

>100_bases
ACATTCTAAGTAAGTTCGATAATAGAAAGAGATAAAGAAAGGCGACCAAAAGGTCGCCTTTTTGTTTTTAGCTGATTACA
ATTCAGCTAATTGATTAATC

Downstream 100 bases:

>100_bases
CCTTAAAAGGCCAGCTCATGCTGGCCTTCTTCTTTAGTAAGGACAGCTTAATGATCCACATTATTACAGGTAGCACCTTA
GGTGGTGCAGAGTACGTTGG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MNALFGFHMLAGTPMTTDTIVAQATAPGRGGVGIIRVSGPLATQVALEVTGKTLKPRYAEYLPFKSHDGIELDQGIALFF
PNPHSFTGEDVLELQGHGGPVVMDMLIKRILAISGVRAARPGEFSERAFLNDKMDLTQAEAIADLIDASSEEAAKSALQS
LQGEFSKRINTLVDSLIYLRIYVEAAIDFPEEEIDFLADGKVSADLQAIIDNLEAVRQEANQGAIMREGMKVVIAGRPNA
GKSSLLNALSGKESAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVEKIGIERAWEEIAQADRVLFMVDGTT
TDATDPKDIWPDFVDRLPDNIGMTVIRNKADQTSEELGICHVNDPTLIRLSAKTGQGVDALRSHLKDCMGFAGGHEGGFM
ARRRHLDALERASEHLDIGQQQLEGYMAGEILAEELRITQQHLNEITGEFSSDDLLGRIFSSFCIGK

Sequences:

>Translated_467_residues
MNALFGFHMLAGTPMTTDTIVAQATAPGRGGVGIIRVSGPLATQVALEVTGKTLKPRYAEYLPFKSHDGIELDQGIALFF
PNPHSFTGEDVLELQGHGGPVVMDMLIKRILAISGVRAARPGEFSERAFLNDKMDLTQAEAIADLIDASSEEAAKSALQS
LQGEFSKRINTLVDSLIYLRIYVEAAIDFPEEEIDFLADGKVSADLQAIIDNLEAVRQEANQGAIMREGMKVVIAGRPNA
GKSSLLNALSGKESAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVEKIGIERAWEEIAQADRVLFMVDGTT
TDATDPKDIWPDFVDRLPDNIGMTVIRNKADQTSEELGICHVNDPTLIRLSAKTGQGVDALRSHLKDCMGFAGGHEGGFM
ARRRHLDALERASEHLDIGQQQLEGYMAGEILAEELRITQQHLNEITGEFSSDDLLGRIFSSFCIGK
>Mature_467_residues
MNALFGFHMLAGTPMTTDTIVAQATAPGRGGVGIIRVSGPLATQVALEVTGKTLKPRYAEYLPFKSHDGIELDQGIALFF
PNPHSFTGEDVLELQGHGGPVVMDMLIKRILAISGVRAARPGEFSERAFLNDKMDLTQAEAIADLIDASSEEAAKSALQS
LQGEFSKRINTLVDSLIYLRIYVEAAIDFPEEEIDFLADGKVSADLQAIIDNLEAVRQEANQGAIMREGMKVVIAGRPNA
GKSSLLNALSGKESAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVEKIGIERAWEEIAQADRVLFMVDGTT
TDATDPKDIWPDFVDRLPDNIGMTVIRNKADQTSEELGICHVNDPTLIRLSAKTGQGVDALRSHLKDCMGFAGGHEGGFM
ARRRHLDALERASEHLDIGQQQLEGYMAGEILAEELRITQQHLNEITGEFSSDDLLGRIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=476, Percent_Identity=34.8739495798319, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI193082988, Length=508, Percent_Identity=33.0708661417323, Blast_Score=225, Evalue=9e-59,
Organism=Homo sapiens, GI193082993, Length=466, Percent_Identity=34.549356223176, Blast_Score=222, Evalue=5e-58,
Organism=Escherichia coli, GI2367268, Length=451, Percent_Identity=75.1662971175166, Blast_Score=691, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=92, Percent_Identity=39.1304347826087, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17507259, Length=459, Percent_Identity=28.322440087146, Blast_Score=162, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6323665, Length=495, Percent_Identity=31.9191919191919, Blast_Score=209, Evalue=5e-55,
Organism=Drosophila melanogaster, GI45550813, Length=480, Percent_Identity=33.3333333333333, Blast_Score=218, Evalue=6e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 50785; Mature: 50785

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALFGFHMLAGTPMTTDTIVAQATAPGRGGVGIIRVSGPLATQVALEVTGKTLKPRYAE
CCCCHHHHHHCCCCCCCHHEEEEECCCCCCCEEEEEECCCCHHHEEEEECCCCCCCCHHH
YLPFKSHDGIELDQGIALFFPNPHSFTGEDVLELQGHGGPVVMDMLIKRILAISGVRAAR
HCCCCCCCCCEECCCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCC
PGEFSERAFLNDKMDLTQAEAIADLIDASSEEAAKSALQSLQGEFSKRINTLVDSLIYLR
CCCCHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYVEAAIDFPEEEIDFLADGKVSADLQAIIDNLEAVRQEANQGAIMREGMKVVIAGRPNA
HHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCC
GKSSLLNALSGKESAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVEKIGIE
CHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHH
RAWEEIAQADRVLFMVDGTTTDATDPKDIWPDFVDRLPDNIGMTVIRNKADQTSEELGIC
HHHHHHHHCCCEEEEEECCCCCCCCCHHCCHHHHHHCCCCCCCCEEECCCCCCHHHCCEE
HVNDPTLIRLSAKTGQGVDALRSHLKDCMGFAGGHEGGFMARRRHLDALERASEHLDIGQ
ECCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCH
QQLEGYMAGEILAEELRITQQHLNEITGEFSSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNALFGFHMLAGTPMTTDTIVAQATAPGRGGVGIIRVSGPLATQVALEVTGKTLKPRYAE
CCCCHHHHHHCCCCCCCHHEEEEECCCCCCCEEEEEECCCCHHHEEEEECCCCCCCCHHH
YLPFKSHDGIELDQGIALFFPNPHSFTGEDVLELQGHGGPVVMDMLIKRILAISGVRAAR
HCCCCCCCCCEECCCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCC
PGEFSERAFLNDKMDLTQAEAIADLIDASSEEAAKSALQSLQGEFSKRINTLVDSLIYLR
CCCCHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYVEAAIDFPEEEIDFLADGKVSADLQAIIDNLEAVRQEANQGAIMREGMKVVIAGRPNA
HHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCC
GKSSLLNALSGKESAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVEKIGIE
CHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHH
RAWEEIAQADRVLFMVDGTTTDATDPKDIWPDFVDRLPDNIGMTVIRNKADQTSEELGIC
HHHHHHHHCCCEEEEEECCCCCCCCCHHCCHHHHHHCCCCCCCCEEECCCCCCHHHCCEE
HVNDPTLIRLSAKTGQGVDALRSHLKDCMGFAGGHEGGFMARRRHLDALERASEHLDIGQ
ECCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCH
QQLEGYMAGEILAEELRITQQHLNEITGEFSSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA