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The map label for this gene is kefF [H]

Identifier: 218704983

GI number: 218704983

Start: 1794936

End: 1795508

Strand: Reverse

Name: kefF [H]

Synonym: ECUMN_1767

Alternate gene names: 218704983

Gene position: 1795508-1794936 (Counterclockwise)

Preceding gene: 218704984

Following gene: 218704982

Centisome position: 34.52

GC content: 51.31

Gene sequence:

>573_bases
ATGAAAAATATCCTTGTTATTTCAGGTCATCCTGAGCTTACCCATTCCGTCGCCAATGCCACTATTCTTGATGAAGTGGA
GACCGCCCTTCCTGATGTTGAGATTCGTCGTCTGGACAGGCTCTATCCGGATGGCAAATTCAACATCGCGGCGGAGCAGG
AAAGCCTGCTCAGGGCCGATGTGATTGTCTGGCAGTTCCCTTTTTCCTGGTATGGGCTGCCAGGATTAATGAAAAAATGG
CTGGACGAGGTCTTTGTTCACGGCTTCGCGCATGGCTCAACGGCGAAACTGGGCGGTAAAAAGCTGATCCTCTCCTTTAC
AACGGGAGCGCCAGAGGAGCTCTATACCGTCGACAGTTTCTTTGGCCATACCATTGAAGAGTATCTTATTCCGTTCGAAA
CCACGGCAAGGCTGTGCAATCTTGAGCTGCTGGCGCCGGTTTATACCTGCGGTATCAGCTATGCCGACCGGGATGCCGAC
AAAATCGCGCAGCAAAAAACGCTTGCCCGGGAACACGCTTCAAGGCTTGTCGAACTGCTCAATTCCGTCGTGAATAATCC
AGAGGGAGAATAA

Upstream 100 bases:

>100_bases
GTTTTTGCCTAAAAGTGAATGAGCCTTTACTGCTCTAAAAAAATAATGTTGTCACTCTATACTGGATTTAATATTCACCA
AAGATGTTTAACTGATTGAA

Downstream 100 bases:

>100_bases
CAATGCAGTATACCCGGCTCGGTAAAAGTGACTTACTTGTTTCCCGCATCTGCATGGGATGCATGGGGTTTGGCGATCCG
TCGACGGGTCAGCATAGCTG

Product: putative NAD(P)H oxidoreductase

Products: NAD(P); reduced acceptor [C]

Alternate protein names: NA

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MKNILVISGHPELTHSVANATILDEVETALPDVEIRRLDRLYPDGKFNIAAEQESLLRADVIVWQFPFSWYGLPGLMKKW
LDEVFVHGFAHGSTAKLGGKKLILSFTTGAPEELYTVDSFFGHTIEEYLIPFETTARLCNLELLAPVYTCGISYADRDAD
KIAQQKTLAREHASRLVELLNSVVNNPEGE

Sequences:

>Translated_190_residues
MKNILVISGHPELTHSVANATILDEVETALPDVEIRRLDRLYPDGKFNIAAEQESLLRADVIVWQFPFSWYGLPGLMKKW
LDEVFVHGFAHGSTAKLGGKKLILSFTTGAPEELYTVDSFFGHTIEEYLIPFETTARLCNLELLAPVYTCGISYADRDAD
KIAQQKTLAREHASRLVELLNSVVNNPEGE
>Mature_190_residues
MKNILVISGHPELTHSVANATILDEVETALPDVEIRRLDRLYPDGKFNIAAEQESLLRADVIVWQFPFSWYGLPGLMKKW
LDEVFVHGFAHGSTAKLGGKKLILSFTTGAPEELYTVDSFFGHTIEEYLIPFETTARLCNLELLAPVYTCGISYADRDAD
KIAQQKTLAREHASRLVELLNSVVNNPEGE

Specific function: Required for full activity of kefC [H]

COG id: COG2249

COG function: function code R; Putative NADPH-quinone reductase (modulator of drug activity B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD(P)H dehydrogenase (quinone) family. KefF subfamily [H]

Homologues:

Organism=Homo sapiens, GI4505415, Length=118, Percent_Identity=32.2033898305085, Blast_Score=71, Evalue=4e-13,
Organism=Homo sapiens, GI156564357, Length=135, Percent_Identity=31.8518518518519, Blast_Score=68, Evalue=5e-12,
Organism=Escherichia coli, GI1786231, Length=177, Percent_Identity=42.3728813559322, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1789750, Length=146, Percent_Identity=37.6712328767123, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1789406, Length=202, Percent_Identity=29.2079207920792, Blast_Score=80, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 1.6.99.- [C]

Molecular weight: Translated: 21261; Mature: 21261

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNILVISGHPELTHSVANATILDEVETALPDVEIRRLDRLYPDGKFNIAAEQESLLRAD
CCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHH
VIVWQFPFSWYGLPGLMKKWLDEVFVHGFAHGSTAKLGGKKLILSFTTGAPEELYTVDSF
HEEEECCCHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
FGHTIEEYLIPFETTARLCNLELLAPVYTCGISYADRDADKIAQQKTLAREHASRLVELL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
NSVVNNPEGE
HHHHCCCCCC
>Mature Secondary Structure
MKNILVISGHPELTHSVANATILDEVETALPDVEIRRLDRLYPDGKFNIAAEQESLLRAD
CCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHH
VIVWQFPFSWYGLPGLMKKWLDEVFVHGFAHGSTAKLGGKKLILSFTTGAPEELYTVDSF
HEEEECCCHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
FGHTIEEYLIPFETTARLCNLELLAPVYTCGISYADRDADKIAQQKTLAREHASRLVELL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
NSVVNNPEGE
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; acceptor [C]

Specific reaction: NAD(P)H2 + acceptor = NAD(P) + reduced acceptor [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA